Journal
EMBO REPORTS
Volume 16, Issue 1, Pages 71-78Publisher
WILEY-BLACKWELL
DOI: 10.15252/embr.201439183
Keywords
C. elegans; nonsense-mediated decay; RNAi screen; smg genes
Categories
Funding
- MRC studentship
- Becas Chile
- MRC core funding
- Wellcome Trust [095518/Z/11/Z, 098410/Z/12/Z]
- Medical Research Council [MC_PC_U127584479, 1137709] Funding Source: researchfish
- Wellcome Trust [095518/Z/11/Z] Funding Source: Wellcome Trust
- MRC [MC_PC_U127584479] Funding Source: UKRI
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Nonsense-mediated mRNA decay (NMD) is a surveillance mechanism that degrades mRNAs harboring premature termination codons (PTCs). We have conducted a genome-wide RNAi screen in Caenorhabditis elegans that resulted in the identification of five novel NMD genes that are conserved throughout evolution. Two of their human homologs, GNL2 (ngp-1) and SEC13 (npp-20), are also required for NMD in human cells. We also show that the C. elegans gene noah-2, which is present in Drosophila melanogaster but absent in humans, is an NMD factor in fruit flies. Altogether, these data identify novel NMD factors that are conserved throughout evolution, highlighting the complexity of the NMD pathway and suggesting that yet uncovered novel factors may act to regulate this process.
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