Review
Genetics & Heredity
Elena Di Nisio, Giuseppe Lupo, Valerio Licursi, Rodolfo Negri
Summary: This review article explores the role of histone lysine methylation changes in regulating the response to radiation-induced genotoxic damage in mammalian cells. It also discusses the effects of histone methyltransferases (HMTs) and histone demethylases (HDMs) on radio-sensitivity of different cell lines, and provides a bioinformatic analysis of mRNA levels of known HMTs and HDMs under different irradiation conditions.
FRONTIERS IN GENETICS
(2021)
Article
Biochemical Research Methods
Samuel J. Hendel, Matthew D. Shoulders
Summary: Directed evolution experiments are traditionally conducted using in vitro systems, bacteria, or yeast, but there is now a shift towards using mammalian cells as the setting for such experiments. This shift has the potential to expand the scope and functionality of evolved targets, but also presents new challenges and opportunities for high-impact research.
Article
Cell Biology
Alexandros Pailas, Konstantina Niaka, Chrysoula Zorzompokou, Petros Marangos
Summary: DNA damage can occur in cells leading to infertility or developmental abnormalities, and it is crucial to understand how oocytes respond to such damage.
Review
Biochemistry & Molecular Biology
Donald H. Atha, Vytas Reipa
Summary: This report describes several methods that could be considered for the production of reference materials for the quantitative measurement of DNA damage in mammalian cells. The focus is on potentially useful methods for assessing DNA damage, particularly DNA strand breaks, in mammalian cells. The advantages, limitations, and concerns with respect to reference material development are discussed. In conclusion, strategies for developing candidate DNA damage reference materials are outlined for adoption by research laboratories in various applications.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Jiangbin Wei, Qiwu Shi, Lidan Xiong, Guang Xin, Tao Yi, Yunqing Xiao, Wanxia Huang
Summary: The study investigated the biological effects of radiation from the SG-III prototype laser facility on HaCat cells and PC12 cells, finding that the damage to cells was dose-dependent and induced DNA damage and apoptotic necrosis. Transcriptome and protein analysis revealed pathways involving proliferation, transformation, necrosis, inflammation response, apoptosis, and DNA damage repair were activated, suggesting potential harm to biological safety.
SCIENTIFIC REPORTS
(2021)
Article
Genetics & Heredity
Yuejing Jiang, Xiaoji Cong, Shangwen Jiang, Ying Dong, Lei Zhao, Yi Zang, Minjia Tan, Jia Li
Summary: AMP-activated protein kinase (AMPK) plays regulatory roles in DNA repair through phosphorylation of various substrates and modulation of histone acetylation. Specifically, AMPK promotes apoptosis through phosphorylation of apoptosis-stimulating of p53 protein 2 (ASPP2), and regulates histone acetylation through phosphorylation of histone deacetylase 9 (HDAC9). Additionally, disrupting histone acetylation enhances the sensitivity of AMPK-deficient cells to irradiation.
GENOMICS PROTEOMICS & BIOINFORMATICS
(2022)
Article
Microbiology
Linda C. Horianopoulos, Christopher W. J. Lee, Kerstin Schmitt, Oliver Valerius, Guanggan Hu, Melissa Caza, Gerhard H. Braus, James W. Kronstad
Summary: This study identifies a nuclear J domain protein, Dnj4, in the fungal pathogen Cryptococcus neoformans, demonstrating its role as a histone chaperone important for maintaining genome integrity and responding to DNA damage. Dnj4 regulates iron homeostasis and plays a crucial role in DNA damage response in C. neoformans. Additionally, Dnj4 functions as a conserved histone chaperone disrupting endogenous histone chaperoning machinery, suggesting its potential in understanding biological processes across different organisms.
Review
Oncology
Takemichi Fukasawa, Atsushi Enomoto, Asako Yoshizaki-Ogawa, Shinichi Sato, Kiyoshi Miyagawa, Ayumi Yoshizaki
Summary: DNA is constantly damaged, and cells have evolved DNA damage response mechanisms to preserve genomic integrity. Kinases play a central role in this response by transmitting the DNA damage signal to various cellular processes. Recently, the role of STK38 in DNA damage signaling has emerged.
Article
Multidisciplinary Sciences
Santanu Mondal, Shu Wang, Yunan Zheng, Sudeshna Sen, Abhishek Chatterjee, Paul R. Thompson
Summary: The study presents a technology for site-specific incorporation of citrulline into proteins in mammalian cells. Incorporating citrulline into proteins can significantly reduce enzyme activity.
NATURE COMMUNICATIONS
(2021)
Review
Medicine, Research & Experimental
Pengfei Ji, Guokun Zhang, Yanan Guo, Haoyun Song, Xinyi Yuan, Xiaohui Hu, Zhao Guo, Peng Xia, Rong Shen, Degui Wang
Summary: DNA damage leads to increased genomic instability and various diseases. Post-translational modifications, including crotonylation, play significant roles in maintaining genomic stability and regulating biological processes and disease development.
Review
Biochemistry & Molecular Biology
Stefania Gonfloni, Carla Jodice, Bianca Gustavino, Elvia Valentini
Summary: Chemotherapy and radiotherapy are common strategies to fight cancer, but they may cause side effects such as premature ovarian insufficiency and infertility in women. Understanding the mechanisms underlying the loss of follicle reserve due to cancer treatments is crucial in developing clinical strategies to protect ovarian reserve. Recent studies have identified the involvement of p53 and CHK2 in the oocyte response to DNA stressors commonly used in cancer treatment. This review explores the molecular mechanisms of the DNA damage stress response and its crosstalk with signaling pathways related to primordial follicle activation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Biochemistry & Molecular Biology
Jianming Wang, Martina Muste Sadurni, Marco Saponaro
Summary: This article focuses on the regulation and repair processes of RNA polymerase II in response to DNA damage. RNA polymerase II is regulated by multi-layer mechanisms during transcription, and transcription-blocking lesions can cause transcriptional repression and require repair.
Article
Multidisciplinary Sciences
Olimpia Alessandra Buoninfante, Bas Pilzecker, Aldo Spanjaard, Daniel de Groot, Stefan Prekovic, Ji-Ying Song, Cor Lieftink, Matilda Ayidah, Colin E. J. Pritchard, Judith Vivie, Kathleen E. Mcgrath, Ivo J. Huijbers, Sjaak Philipsen, Marieke von Lindern, Wilbert Zwart, Roderick L. Beijersbergen, James Palish, Paul C. M. van den Berk, Heinz Jacobs
Summary: DNA damage poses a threat to genomic integrity and leads to stem cell failure. Cells use DNA damage tolerance (DDT), regulated by PCNA ubiquitination and REV1, to bypass genotoxic lesions during replication. While DDT is conserved in all domains of life, its relevance in mammals has been unclear. Our study demonstrates that inactivation of both PCNA ubiquitination and REV1 results in embryonic and adult lethality, as well as accumulation of DNA damage in hematopoietic stem and progenitor cells (HSPCs) which ultimately leads to their depletion. This highlights the crucial importance of DDT in the maintenance of stem cell compartments and mammalian life under unperturbed conditions.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Cell Biology
Kenya Bonitto, Kirthana Sarathy, Kaiser Atai, Mithun Mitra, Hilary A. Coller
Summary: This article discusses the epigenetic changes during cell entry into and exit from quiescence, examines the role of different histone marks during quiescence, and explores the possibility of a quiescence histone code.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Genetics & Heredity
John C. Schimenti, Rui Huang, Liangdao Li, Ryan James
Summary: The maintenance of tissue homeostasis by stem cells relies on genome maintenance and regulation of damage response. Different types of stem cells employ various strategies to cope with DNA damage and meet the demands of tissue maintenance.
ANNUAL REVIEW OF GENETICS
(2022)
Article
Multidisciplinary Sciences
David Sitbon, Ekaterina Boyarchuk, Florent Dingli, Damarys Loew, Genevieve Almouzni
NATURE COMMUNICATIONS
(2020)
Article
Cell Biology
Brendan Evano, Sara Khalilian, Gilles Le Carrou, Genevieve Almouzni, Shahragim Tajbakhsh
Article
Multidisciplinary Sciences
Nikolaus Rajewsky, Genevieve Almouzni, Stanislaw A. Gorski, Stein Aerts, Ido Amit, Michela G. Bertero, Christoph Bock, Annelien L. Bredenoord, Giacomo Cavalli, Susanna Chiocca, Hans Clevers, Bart De Strooper, Angelika Eggert, Jan Ellenberg, Xose M. Fernandez, Marek Figlerowicz, Susan M. Gasser, Norbert Hubner, Jorgen Kjems, Juergen A. Knoblich, Grietje Krabbe, Peter Lichter, Sten Linnarsson, Jean-Christophe Marine, John C. Marioni, Marc A. Marti-Renom, Mihai G. Netea, Dorthe Nickel, Marcelo Nollmann, Halina R. Novak, Helen Parkinson, Stefano Piccolo, Ines Pinheiro, Ana Pombo, Christian Popp, Wolf Reik, Sergio Roman-Roman, Philip Rosenstiel, Joachim L. Schultze, Oliver Stegle, Amos Tanay, Giuseppe Testa, Dimitris Thanos, Fabian J. Theis, Maria-Elena Torres-Padilla, Alfonso Valencia, Celine Vallot, Alexander van Oudenaarden, Marie Vidal, Thierry Voet
Article
Biochemistry & Molecular Biology
Julia Torne, Dominique Ray-Gallet, Ekaterina Boyarchuk, Mickael Garnier, Patricia Le Baccon, Antoine Coulon, Guillermo A. Orsi, Genevieve Almouzni
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Song My Hoang, Nicole Kaminski, Ragini Bhargava, Jonathan Barroso-Gonzalez, Michelle L. Lynskey, Laura Garcia-Exposito, Justin L. Roncaioli, Anne R. Wondisford, Callen T. Wallace, Simon C. Watkins, Dominic I. James, Ian D. Waddell, Donald Ogilvie, Kate M. Smith, Felipe da Veiga Leprevost, Dattatreya Mellacharevu, Alexey I. Nesvizhskii, Jianfeng Li, Dominique Ray-Gallet, Robert W. Sobol, Genevieve Almouzni, Roderick J. O'Sullivan
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2020)
Article
Oncology
Roman M. Chabanon, Daphne Morel, Thomas Eychenne, Leo Colmet-Daage, Ilirjana Bajrami, Nicolas Dorvault, Marlene Garrido, Cornelia Meisenberg, Andrew Lamb, Carine Ngo, Suzanna R. Hopkins, Theodoros Roumeliotis, Samuel Jouny, Clemence Henon, Asuka Kawai-Kawachi, Clemence Astier, Asha Konde, Elaine Del Nery, Christophe Massard, Stephen J. Pettitt, Raphael Margueron, Jyoti S. Choudhary, Genevieve Almouzni, Jean-Charles Soria, Eric Deutsch, Jessica A. Downs, Christopher J. Lord, Sophie Postel-Vinay
Summary: Inactivation of PBRM1 is common in cancer, including ccRCC. Studies show that PARP and ATR inhibitors are synthetic lethal with PBRM1 deficiency, providing a basis for using these inhibitors in PBRM1-defective cancers.
Correction
Multidisciplinary Sciences
Nikolaus Rajewsky, Genevieve Almouzni, Stanislaw A. Gorski, Stein Aerts, Ido Amit, Michela G. Bertero, Christoph Bock, Annelien L. Bredenoord, Giacomo Cavalli, Susanna Chiocca, Hans Clevers, Bart De Strooper, Angelika Eggert, Jan Ellenberg, Xose M. Fernandez, Marek Figlerowicz, Susan M. Gasser, Norbert Hubner, Jorgen Kjems, Jurgen A. Knoblich, Grietje Krabbe, Peter Lichter, Sten Linnarsson, Jean-Christophe Marine, John C. Marioni, Marc A. Marti-Renom, Mihai G. Netea, Dorthe Nickel, Marcelo Nollmann, Halina R. Novak, Helen Parkinson, Stefano Piccolo, Ines Pinheiro, Ana Pombo, Christian Popp, Wolf Reik, Sergio Roman-Roman, Philip Rosenstiel, Joachim L. Schultze, Oliver Stegle, Amos Tanay, Giuseppe Testa, Dimitris Thanos, Fabian J. Theis, Maria-Elena Torres-Padilla, Alfonso Valencia, Celine Vallot, Alexander van Oudenaarden, Marie Vidal, Thierry Voet
Article
Biology
Daniel Jeffery, Alberto Gatto, Katrina Podsypanina, Charlene Renaud-Pageot, Rebeca Ponce Landete, Lorraine Bonneville, Marie Dumont, Daniele Fachinetti, Genevieve Almouzni
Summary: The study establishes an inducible and reversible CENP-A overexpression system, combined with a switch in p53 status in human cell lines, revealing p53 as a key determinant of how CENP-A impacts cell state, cell identity, and therapeutic response. The findings show that CENP-A overexpression promotes senescence and radiosensitivity when p53 is functional, while promoting epithelial-mesenchymal transition when p53 is inactivated.
COMMUNICATIONS BIOLOGY
(2021)
Editorial Material
Genetics & Heredity
Ines Pinheiro, Maria-Elena Torres-Padilla, Genevieve Almouzni
Article
Oncology
Pierre Verrelle, Didier Meseure, Frederique Berger, Audrey Forest, Renaud Leclere, Andre Nicolas, Emilie Fortas, Xavier Sastre-Garau, Marick Lae, Sabah Boudjemaa, Rodrigue Mbagui, Valentin Calugaru, Dalila Labiod, Leanne De Koning, Genevieve Almouzni, Jean-Pierre Quivy
Summary: The subnuclear distribution of CENP-A serves as an independent predictive marker for local disease control and curability by chemoradiation therapy in locally advanced head and neck squamous cell cancer patients, providing a reliable marker for precision medicine. This study highlights the potential clinical applicability of CENP-A labeling as a cost-effective marker compatible with clinical time constraints during therapy.
Article
Biochemistry & Molecular Biology
Alberto Gatto, Audrey Forest, Jean-Pierre Quivy, Genevieve Almouzni
Summary: This study reveals the dual deposition mode of histone variants H3.1 and H3.3 in human cells during S phase, which ensures a stable marking with H3.3 flanked on both sides by H3.1. The H3.1/H3.3 boundaries correspond to the initiation zones of early origins, and the HIRA-dependent deposition of H3.3 plays a crucial role in preserving these boundaries and contributing to the chromatin-based definition of early replication zones.
Article
Clinical Neurology
Laure Gallay, Cecile Fermon, Lola Lessard, Michele Weiss-Gayet, Sebastien Viel, Nathalie Streichenberger, Armelle Corpet, Remi Mounier, Cyril Gitiaux, Guy Mouchiroud, Benedicte Chazaud
Summary: This study investigated the proliferative properties of muscle stem cells (MuSCs) in patients with dermatomyositis and the role of type I interferon (IFN-I) in this process. Results indicate that autocrine IFN-I signaling inhibits MuSC expansion, leading to muscle repair deficit.
Article
Biology
Olivier Binda, Franceline Juillard, Julia Novion Ducassou, Constance Kleijwegt, Genevieve Paris, Andreanne Didillon, Faouzi Baklouti, Armelle Corpet, Yohann Coute, Jocelyn Cote, Patrick Lomonte
Summary: Spinal muscular atrophy (SMA) is a genetic disease that leads to infant mortality, but recent advances in gene therapy have shown promise. In this study, researchers identified abnormal protein-protein interactions between SMN and key neuronal factors in cases of SMN1 mutation. They also discovered the involvement of SMN in the formation of membraneless organelles and localized translation in motor neurons.
LIFE SCIENCE ALLIANCE
(2022)
Article
Biology
Constance Kleijwegt, Florent Bressac, Coline Seurre, Wilhelm Bouchereau, Camille Cohen, Pascale Texier, Thomas Simonet, Laurent Schaeffer, Patrick Lomonte, Armelle Corpet
Summary: Promyelocytic leukemia nuclear bodies (PML NBs) play a crucial role in genome function by regulating the distribution of HIRA and histone dynamics. Additionally, they regulate the transcription of interferon-stimulated genes (ISGs) and H3.3 deposition mediated by HIRA during inflammatory response.
Article
Biology
Olivier Binda, Aime Boris Kimenyi Ishimwe, Maxime Galloy, Karine Jacquet, Armelle Corpet, Amelie Fradet-Turcotte, Jocelyn Cote, Patrick Lomonte
Summary: Spinal muscular atrophy, caused by depleted levels of SMN protein due to deletion or mutation of the SMN1 gene, is the leading genetic cause of infant mortality. This study shows that SMN also associates with H3K79me1 histone modification, making it the first protein to recognize both methylated arginine and lysine residues. Mutational analysis reveals that SMNTUDOR associates with H3 through an aromatic cage, and most SMNTUDOR mutants found in patients fail to associate with H3K79me1.
LIFE SCIENCE ALLIANCE
(2023)
