Article
Chemistry, Analytical
Christopher Kenji Brenden, Hrishikesh Iyer, Yan Zhang, Sungho Kim, Weihua Shi, Yurii A. Vlasov
Summary: This paper introduces an implantable electrochemical sensor that integrates ultra-low flow silicon microfluidic channels for protection from foulants and in-situ calibration. The small device size allows integration into implantable sampling probes for monitoring chemical concentrations in biological tissues. Numerical analysis confirms near complete electrolysis in the thin-layer flow regime.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Review
Chemistry, Analytical
Sheng Yan, Dan Yuan
Summary: The development of microfluidic technologies, particularly microflow cytometry with 3D focusing capabilities, has shown great promise in single-cell analysis, offering a wide range of applications in biology and medicine.
Review
Nanoscience & Nanotechnology
Daniel Vera, Maria Garcia-Diaz, Nuria Torras, Mar Alvarez, Rosa Villa, Elena Martinez
Summary: Researchers have integrated hydrogels into microfluidic setups to develop tissue barrier-on-chips that accurately account for different cellular and acellular compartments, including the cellular components and functionality of in vivo tissues. These platforms have the potential to greatly improve the predictive capacities of in vitro systems in applications such as drug development or disease modeling, but face challenges in microfabrication.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biochemical Research Methods
Jorvani Cruz Villarreal, Rory Kruithoff, Ana Egatz-Gomez, Paul D. Coleman, Robert Ros, Todd R. Sandrin, Alexandra Ros
Summary: Understanding cell-to-cell variation at the molecular level is critical for research and this study presents a microfluidic platform combined with MALDI-TOF-MS for the detection and quantification of targeted proteins from small cell ensembles. The integrated on-chip approach allows for protein quantification from cell lysate and demonstrates promising potential for single-cell analysis.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2022)
Article
Materials Science, Multidisciplinary
Simon Raffy, Etienne Palleau, Brice Calvignac, Guillaume Brotons, Guillaume Lefebvre, Nicolas Rolley, Sebastien Teychene, Bernard Viguier, Sara Castano Cerezo, Gilles Truan, Laurence Ressier
Summary: The choice of materials in chip fabrication for microfluidics is crucial, and this study proposes a versatile approach using a combination of epoxy materials to fabricate microfluidic chips with various potentialities. The chips demonstrate good chemical resistance, optical transparency, biocompatibility, high pressure capability, and integration of high aspect ratio channels, making them promising for prototyping in a wide range of applications and fundamental studies.
ACS APPLIED POLYMER MATERIALS
(2021)
Review
Chemistry, Analytical
Alessio Meggiolaro, Valentina Moccia, Paola Brun, Matteo Pierno, Giampaolo Mistura, Valentina Zappulli, Davide Ferraro
Summary: Extracellular vesicles (EVs) are receiving considerable attention in the biological and medical fields due to their role as natural carriers of genetic information and their potential as diagnostic and prognostic biomarkers in various diseases. However, conventional methods for EV isolation have limitations, and microfluidic approaches have been proposed as a solution. This review summarizes the most important microfluidic-based devices for EV isolation and their advantages and disadvantages compared to existing technology.
Review
Polymer Science
Anthony Tony, Ildiko Badea, Chun Yang, Yuyi Liu, Garth Wells, Kemin Wang, Ruixue Yin, Hongbo Zhang, Wenjun Zhang
Summary: This paper provides a comprehensive review on the fabrication of PDMS microfluidic devices using additive manufacturing (AM) processes. Two approaches, direct printing and indirect printing, are classified. The focus of the study is on the printed mold approach, which involves casting PDMS materials with a printed mold. The paper also identifies knowledge gaps, proposes future work, and develops a novel classification of AM processes.
Article
Engineering, Biomedical
Naim Yagiz Demir, Ibrahim Bozyel, Baran Erman, Dincer Gokcen, Memed Duman
Summary: The aim of this study was to stimulate platelets without using chemical stimulants. Two mechanical platelet activation methods were proposed: shear-induced platelet activation using a microfluidic chip and ultrasound-assisted platelet activation using a piezo-based device. The results showed that the microfluidic chip with 8-nodes achieved the highest platelet activation rate (72.7%), while the ultrasound-assisted device achieved a 32.4% activation rate. These devices can be used to enhance the effect of PRP treatment and reduce adverse immune reactions.
IEEE TRANSACTIONS ON BIOMEDICAL ENGINEERING
(2023)
Article
Chemistry, Analytical
Chayakorn Petchakup, Paul Edward Hutchinson, Hui Min Tay, Sheng Yuan Leong, King Ho Holden Li, Han Wei Hou
Summary: This study presents a new method for studying lymphocytes based on impedance, which can quickly screen the responses of specific antigen-specific T lymphocytes and be used for the characterization of host immunity and the diagnosis of infectious diseases.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Pharmacology & Pharmacy
Benzion Amoyav, Yoel Goldstein, Eliana Steinberg, Ofra Benny
Summary: Microfluidics research has shown exponential growth in various applications, with the combination of 3D printing technology providing an efficient platform for particle production, reducing costs and time. The one-step fabrication process for a microfluidic chip for drug dissolution assays has advantages in terms of encapsulation efficiency and drug content compared to traditional batch formulations.
Article
Biochemical Research Methods
Tyler J. Allcroft, Jessica T. Duong, Per Sebastian Skardal, Michelle L. Kovarik
Summary: Single-cell measurements often show high levels of variation between cells, but there is limited understanding of the sources of this variation. In this study, oxidative stress levels in individual Dictyostelium discoideum cells were analyzed to investigate population-level variation and its sources. The results revealed that cell cycle position was a major contributor to heterogeneity in oxidative stress, with mitotic cells exhibiting higher levels of reactive oxygen species than interphase cells.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Review
Biochemical Research Methods
Carolus Vitalis, Tobias Wenzel
Summary: Microfluidic droplet screens are an innovative platform for high-throughput biotechnology, contributing to advancements in discovery, product optimization, and analysis. This review focuses on the emerging trends of interaction assays in microfluidic droplets, highlighting their unique suitability. These assays encompass various biological entities and demonstrate versatility and scope. Recent advancements have expanded the scale of bioanalysis and biotechnological product design. Additionally, the review discusses pioneering breakthroughs in extending droplet-based screens into new domains, such as cargo delivery within human bodies and the development of responsive droplet structures.
CURRENT OPINION IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Pedro Gonzalez-Losada, Martina Freisa, Claire Poujouly, Jean Gamby
Summary: This article presents the design, fabrication, and characterization of a microfluidic device and electronic system for multiplexed electrochemical measurements of synthetic miRNA strands. The results show that this system is as reliable as conventional methods, while reducing complexity and providing an integrated, multiplexed, and portable miRNA quantification system.
Article
Chemistry, Analytical
Diwakar M. Awate, Cicero C. Pola, Erica Shumaker, Carmen L. Gomes, Jaime J. Juarez
Summary: Despite the limitations of flow cytometers, a 3D-printed imaging platform (3DPIP) has been developed to accurately count particles and conduct fluorescence microscopy efficiently. The 3DPIP utilizes a custom particle counter code and machine vision-based algorithm to identify and count particles, showing comparable accuracy to commercial particle counters. Additionally, the platform can perform static experiments and flow-through experiments effectively, making it a promising tool for use in resource-limited clinical environments.
Article
Optics
Daniele Pirone, Massimiliano Maria Villone, Pasquale Memmolo, Zhe Wang, Volodymyr Tkachenko, Wen Xiao, Leiping Che, Lu Xin, Xiaoping Li, Feng Pan, Pietro Ferraro, Pier Luca Maffettone
Summary: In the application of digital holography (DH) for lab on chip, cells passing through the microscope's field of view can be detected and analyzed even if they are flowing at different depths. This is achieved by retrieving in-focus imaging of each cell through DH. The preferred configuration for DH in flow-cytometry mode involves the rotation of cells along the microfluidic channel, allowing for the retrieval of 3D refractive index maps of flowing cells. Hydrodynamic interactions were investigated and found to have a quantitative effect on cell rotation but not on cell deformation, with the effect being negligible in terms of tomogram recovery.
OPTICS AND LASERS IN ENGINEERING
(2022)
