Review
Chemistry, Applied
Petr Tuma, Frantisek Opekar, Pavel Dlouhy
Summary: This paper provides a comprehensive survey of using capillary and microchip electrophoresis with contactless conductivity detection for the analysis of drinking water, beverages, and food items. It highlights the analysis of food on microchips and modern trends such as dual-opposite injection. CE/(CD)-D-4 is characterized by its universality, analysis speed, simple sample preparation, and minimal sample consumption.
Review
Chemistry, Analytical
Theo Lienard-Mayor, Myriam Taverna, Stephanie Descroix, Thanh Duc Mai
Summary: This study provides a comprehensive overview of different strategies for combining droplet-based sample handling and preparation with electrophoretic separation. It focuses on three principal interfacing strategies, including droplets in immiscible phases, digital microfluidics with electrowetting-on-dielectric principle, and inkjet droplet generation. Different instrumental developments, pros and cons of designs, and application demonstrations of droplet-interfaced electrokinetic strategies are discussed.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Hongyu Nie, Zhihui Li, Xiaokun Wang, Rongmeng Gu, Hongyan Yuan, Yong Guo, Dan Xiao
Summary: In this study, a multi-input capacitively coupled contactless conductivity detector (MICD)-D-4 was proposed with high sensitivity, and further improved by combining it with differential detection to form (DFMICD)-D-4. The new detector showed improved performance compared to traditional single-input and double-input detectors, with a higher response-to-baseline ratio and a low limit of detection (0.7 nM). It can be applied to the detection of low-conductivity solutions and total dissolved solids (TDS) analysis, demonstrating its versatility and superiority over standard conductivity meters.
Article
Chemistry, Analytical
Thushara N. Samarasinghe, Yong Zeng, Carey K. Johnson
Summary: The separation of a set of model proteins was tested on a microchip electrophoresis analytical platform, with the best separation and resolution achieved on a silica-nanoparticle colloidal array chip.
JOURNAL OF SEPARATION SCIENCE
(2021)
Review
Biochemical Research Methods
Elahe Naghdi, Griffin E. Moran, Marika Ejby Reinau, Wim De Malsche, Christian Neusuess
Summary: This paper reviews the basic concepts and latest advances in the technology of microchip electrophoresis (ME) coupled to mass spectrometry (MS) and summarizes the recent applications of ME-MS in various fields. The benefits and limitations of ME-MS compared to alternative techniques are discussed.
Review
Chemistry, Analytical
Petr Tuma
Summary: This review article covers the historical development and current techniques of using capillary and chip electrophoresis with contactless conductivity detection for amino acid determination. It discusses theory of conductivity detection, optimization of background electrolyte, chiral separation, multidimensional separation, and the use of multidetection systems. Additionally, it highlights clinical applications and modern sample treatment techniques.
Review
Biochemical Research Methods
Zhao Meng, Junmin Bi, Qianqian Zhang, Hang Ren, Weidong Qin
Summary: The review discusses the applications of nanomaterials in capillary electrophoresis (CE) and microchip capillary electrophoresis (MCE) to enhance sensitivity of various detection techniques. It provides an overview of the types of nanomaterials used, detection mechanisms, analytical performance, and applications.
Review
Chemistry, Analytical
Petr Tuma
Summary: Contactless conductivity detection (C4D) is a universal detection technique that plays a significant role in the analysis of clinical samples. It is particularly sensitive to low molecular weight biogenic substances and can directly quantify them in their native form, making it widely applicable.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Saara Mikkonen, Leila Josefsson, Meeri E-L Makinen, Veronique Chotteau, Asa Emmer
Summary: CE and MCE methods were developed and applied for the analysis of amino acids in cell culture supernatant. The results showed that these methods were sensitive and the relative concentration changes agreed well with HPLC results. In addition, CE and MCE methods were faster and resulted in lower sample and reagent consumption.
BIOTECHNOLOGY JOURNAL
(2022)
Article
Chemistry, Analytical
Sille Stepanova, Vaclav Kasicka
Summary: This review provides an overview of recent advances and applications of capillary electrophoresis and microchip capillary electrophoresis methods in proteomics and peptidomics. It covers topics such as sample preparation, hyphenation with mass spectrometry, and multidimensional separations. New developments in both bottom-up and top-down approaches in proteomics are discussed, along with various applications in protein posttranslational modifications, biological and biochemical research, clinical peptidomics and proteomics, and food analysis.
JOURNAL OF SEPARATION SCIENCE
(2023)
Article
Food Science & Technology
Dan Li, Mingwu Zang, Shouwei Wang, Kaihua Zhang, Zheqi Zhang, Xiaoman Li, Jinchun Li, Wenping Guo
Summary: This study examined food fraud issues in rejected imported foods in China from 2009 to 2019, revealing that 30.35% of rejected imported foods involved food fraud. Processed foods accounted for the highest proportion of food fraud. The analysis showed that foods involved in fraud mainly originated from 112 countries and regions, with entry ports distributed in 24 provinces.
Review
Biotechnology & Applied Microbiology
Amadeo Sena-Torralba, Yulieth D. Banguera-Ordonez, Laia Mira-Pascual, Angel Maquieira, Sergi Morais
Summary: To decentralize health care, the development of point-of-care (PoC) assays has gained attention. The lateral flow immunoassay (LFIA) is seen as a promising bioanalytical method due to its low cost and single-step detection process. However, its limited sensitivity and inability to detect disease biomarkers at clinically relevant levels have limited its application. This review explores the potential of merging different electrokinetic phenomena into paper-based assays to enhance their analytical performance.
TRENDS IN BIOTECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Samyukta Sah, Sylvia R. Yun, David A. Gaul, Andro Botros, Eun Young Park, Olga Kim, Jaeyeon Kim, Facundo M. Fernandez
Summary: In this study, a targeted microchip CE-HRMS method was developed to analyze multiple metabolites in mouse serum samples, revealing metabolic alterations during the development and progression of high-grade serous carcinoma.
Article
Medical Laboratory Technology
Mehmet Senes, Medine Alpdemir, Gul Kirtil, Elmas Ogus, Emel Colak Samsum, Mehmet Fatih Alpdemir
Summary: This study investigates the effect of NULM and IVLE on SPEP. The results show significant interference in albumin and gamma fractions in NULM-added SPEP. Therefore, it is crucial to use NULM instead of IVLE solutions in laboratory tests and calculate fraction concentrations with total protein concentration for evaluating SPEP results.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Review
Biochemical Research Methods
Abdallah M. Zeid, Islam M. Mostafa, Baohua Lou, Guobao Xu
Summary: Pathogenic bacteria and viruses are the main causes of infectious diseases worldwide. Early diagnosis plays a critical role in controlling their spread and treating infections. The innovation of smart sensing platforms, including nanosensors, is essential for isolating patients and providing suitable treatment strategies. Nanosensors offer high sensitivity, specificity, portability, and fast analysis, and they can be used for pathogen detection in biological matrices. The integration of nanosensors with artificial intelligence enables precise detection of pathogenic bacteria and viruses in biological samples.
Article
Biochemical Research Methods
Z. W. Zhong, R. G. Wu, Z. P. Wang, H. L. Tan
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2015)
Article
Nanoscience & Nanotechnology
Seyed Ali Mousavi Shaegh, Zhenfeng Wang, Sum Huan Ng, Ruige Wu, Huu Tuan Nguyen, Leon Cong Zhi Chan, Alicia Guek Geok Toh, Zhiping Wang
MICROFLUIDICS AND NANOFLUIDICS
(2015)
Article
Biochemical Research Methods
Ruige Wu, Y. P. Seah, Zhiping Wang
JOURNAL OF CHROMATOGRAPHY A
(2016)
Article
Biochemical Research Methods
M. Alberti, Y. Dancik, G. Sriram, B. Wu, Y. L. Teo, Z. Feng, M. Bigliardi-Qi, R. G. Wu, Z. P. Wang, P. L. Bigliardi
Article
Biochemical Research Methods
V. B. Varma, R. G. Wu, Z. P. Wang, R. V. Ramanujan
Article
Materials Science, Multidisciplinary
Gopu Sriram, Massimo Alberti, Yuri Dancik, Bo Wu, Ruige Wu, Zhaoxu Feng, Srinivas Ramasamy, Paul Lorenz Bigliardi, Mei Bigliardi-Qi, Zhiping Wang
Article
Biochemical Research Methods
Ruige Wu, Zhiping Wang, Wenfeng Zhao, William Shu-Biu Yeung, Ying Sing Fung
JOURNAL OF CHROMATOGRAPHY A
(2013)
Article
Multidisciplinary Sciences
Z. M. Wang, R. G. Wu, Z. P. Wang, R. V. Ramanujan
SCIENTIFIC REPORTS
(2016)
Article
Engineering, Electrical & Electronic
Weidong Zhou, Ruige Wu, Suhanya Duraiswamy, Wei Wang, Liang Zhu, Zhiping Wang
Summary: The study introduced a microfluidic cartridge for rapid isolation of Staphylococcus aureus in blood without the need for culture, followed by diagnosis through multiplex PCR. The cartridge design, utilizing magnetic beads and ultrasonication, successfully isolated the bacteria from large blood volumes, demonstrating its potential for efficient S. aureus detection.
JOURNAL OF MICROMECHANICS AND MICROENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Xiaoying Qi, Ruige Wu, Boon Keng Lok, Wai Tat Kerk, Steve Foo Khuen Lai, Wei Fan, Kanyi Pu
Summary: This study presents a novel biomolecule-interactive flexible light emitting capacitor (LEC) display capable of dynamic and quantitative visualization of biomolecules through naked-eye detectable electroluminescence. The BIO-LEC features a simple stimuli response principle, no labeling requirement in test conditions, effective sampling method through an integrated microfluidic chip, sensitive detection limit for clinically relevant levels of heparin concentrations, and high compliance with industrial manufacturing standards.
Review
Biotechnology & Applied Microbiology
Li Ling Tan, Nitin Loganathan, Sushama Agarwalla, Chun Yang, Weiyong Yuan, Jasmine Zeng, Ruige Wu, Wei Wang, Suhanya Duraiswamy
Summary: This paper introduces the digital polymerase chain reaction (dPCR) technology and its applications in molecular diagnostics. The dPCR technology has advantages of high sensitivity, precision, and specificity, and has been commercialized by several companies. The paper compares the working principles, technical aspects, usability, costs, and applications of dPCR devices from different companies, and discusses the future trends of dPCR devices in the next few years.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2023)
Article
Chemistry, Analytical
Wei Hua, Wei Wang, Weidong Zhou, Ruige Wu, Zhenfeng Wang
Summary: This paper studies modifications made to the Bosanquet equation in order to improve its fitting performance to experimental observations. By including factors such as the outflow of air, the increase in hydraulic resistance due to surface roughness, and the variation of advancing contact angle, the modified Bosanquet equation significantly improved the fitting quality to the experimental results.
Review
Chemistry, Analytical
Yu Fang, Ruige Wu, Joo Mong Lee, Ling Hou Melinda Chan, Kok Yen Jerry Chan
Summary: The field of assisted reproductive technology (ART) has been constantly improving, and microfluidic technology has the potential to play a transformative role in optimizing embryo development. Microfluidics can be integrated into various stages of embryo production, enabling miniaturization, integration, and automation. Progress in 3D printing technology, organ-on-a-chip, alternative materials, and integrated microfluidic operational units will drive the development of this technology. An ideal microfluidic system would incorporate oocyte processing, sperm processing, and embryo culture into a single compact device. This article reviews the recent applications of microfluidic technologies in IVF procedures and discusses the challenges and potentials for their application in ART.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Wei Hua, Weidong Zhou, Wei Wang, Zhenfeng Wang, Ruige Wu, Liang Zhu
Summary: This study examines the efficiency of capillary pump in circular, square, and rectangular channels through experimentally verified results and analytical analysis. It is revealed that the optimized equations show the most efficient fluid pumping in square shaped channels compared to rectangular and circular ones.
SN APPLIED SCIENCES
(2021)
