Article
Biochemical Research Methods
Qian Kong, Mi Ke, Yicheng Weng, Yunqiu Qin, An He, Pengfei Li, Zongwei Cai, Ruijun Tian
Summary: Tyrosine phosphorylation (pTyr)-dependent signaling pathways are important in biological processes. In this study, a proximity proteomic strategy was used to analyze these signaling complexes and associated phosphorylation sites.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Food Science & Technology
Raquel Rodriguez-Vazquez, Daniel Mouzo, Carlos Zapata
Summary: Protein phosphorylation is a reversible post-translational modification that plays a major regulatory role in many cellular processes. However, analyzing phosphoproteins remains a challenging task in proteome research. This study compares 2-DE phosphoproteome profiles using Pro-Q Diamond phosphoprotein stain and HF-P dephosphorylation method. The results show that the HF-P method detects a significantly higher number of phosphoproteins than Pro-Q DPS, including low-abundant and low-phosphorylated ones. This is the first global assessment of meat phosphoproteome maps using HF-P, providing a new approach for 2-DE gel-based phosphoproteome analysis.
Article
Chemistry, Physical
Ya-Ping Li, Liu Yang, Hao-Dong Liu, Na Jiao, Mei-Yan Ni, Ning Hao, Hong-Yan Lu, Ping Zhang
Summary: In recent years, researchers have been focused on finding high-temperature two-dimensional (2D) superconductors at atmospheric pressure, following the discovery of three-dimensional (3D) high-temperature superconductors at ultrahigh pressure. In this study, the superconductivity of a hydrogenated monolayer phosphorus carbide was investigated using first-principles calculations. It was found that hydrogenation transforms the monolayer from a semiconductor to a metal, and the electron-phonon coupling mechanism contributes to the increased critical temperature of the material, with the Tc reaching 31.0K and even higher when a biaxial tensile strain is applied.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Immunology
Rosanna Herold, Lea Denzer, Walter Muranyi, Carolin Stump-Guthier, Hiroshi Ishikawa, Horst Schroten, Christian Schwerk
Summary: This study investigates the phosphoproteome of human epithelial choroid plexus cells during infection with Neisseria meningitidis and reveals that the capsule-deficient mutant has a stronger impact on the cells' phosphoproteome.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hoora Shaghaghi, Rachel Para, Cara Tran, Jesse Roman, Yemaiza Ojeda-Lassalle, Jianxin Sun, Freddy Romero, Ross Summer
Summary: Research has shown that mitochondrial respiratory dysfunction in lung epithelial cells during pulmonary fibrosis modeling experiments is influenced by different metabolic fuels. Results indicate a significant reduction in mitochondrial respiration when glucose is the primary fuel, while the addition of glutamine can protect mitochondrial respiration in lung epithelial cells, reducing DNA damage and cell death.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Agriculture, Dairy & Animal Science
X. W. Zhao, H. L. Zhu, Y. X. Qi, T. Wu, D. W. Huang, G. L. Cheng, Y. X. Yang, D. P. Bu, H. Hu, L. F. Meng
Summary: Using mass spectrometry, this study investigated the changes in the phosphoproteome profile in the small intestine of neonatal calves and found that protein phosphorylation plays a critical role in nutrient transport and immune response in these calves.
JOURNAL OF DAIRY SCIENCE
(2022)
Article
Chemistry, Analytical
Taran Driver, Nikhil Bachhawat, Ruediger Pipkorn, Leszek J. Frasinski, Jon P. Marangos, Marina Edelson-Averbukh, Vitali Averbukh
Summary: The study introduces a protein database search engine based on 2D-PC-MS method, which can accurately identify protein sequences by matching theoretical and experimentally detected correlating fragments, with high structural specificity. This search engine is not only suitable for peptide identification, but also for intact protein identification.
ANALYTICAL CHEMISTRY
(2021)
Article
Pathology
Jennifer Hinley, Rosalind Duke, Jessica Jinks, Jens Stahlschmidt, David Keene, Raimondo M. Cervellione, Imran Mushtaq, Paolo De Coppi, Massimo Garriboli, Jennifer Southgate
Summary: Bladder exstrophy (BEX) is a rare developmental abnormality that results in an open bladder plate. This study found that patient-derived BEX epithelial cells in vitro can form a barrier-forming epithelium under specific conditions. The expression of squamous KRT14 transcript is inversely related to barrier development, and transcriptional drivers of urothelial differentiation show reduced expression in squamous BEX cultures.
AMERICAN JOURNAL OF PATHOLOGY
(2022)
Article
Multidisciplinary Sciences
Fernanda Perez-Verdugo, German Reig, Mauricio Cerda, Miguel L. Concha, Rodrigo Soto
Summary: By analyzing the changes in epithelial tissue morphology, we can globally fit the parameters of the vertex model and capture apical contractile pulses using perimeter activity and area plasticity.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2022)
Article
Biochemistry & Molecular Biology
Jing Chen, Qi Li, Yifan Hong, Xiazhu Zhou, Chengjun Yu, Xiaomao Tian, Jie Zhao, Chunlan Long, Lianju Shen, Shengde Wu, Guanghui Wei
Summary: The mechanism of bladder fibrosis in neurogenic bladder remains unclear. This study found that the NF-kappa B signaling pathway and inflammation are have elevated levels in bladder fibrosis induced by spinal cord injury. Experiments with a urinary epithelial cell line further confirmed the involvement of NF-kappa B signaling pathway and pyroptosis in bladder fibrosis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Analytical
Adlina Roslan, Diana Suhaiza Said, Nurshahira Sulaiman, Khairul Asri Mohd Ghani, Armania Nurdin
Summary: Through global proteomics analysis, we identified the highly expressed CD147 and caveolin-1 in bladder cancer cells, suggesting the potential of CD147 as a biomarker for bladder cancer and its role in improving drug sensitivity and treatment outcomes. The identification of membrane proteins associated with bladder cancer recurrence is crucial for understanding the mechanisms underlying drug resistance to chemotherapy.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Shalini Jaswal, Vijay Anand, Syed Azmal Ali, Manoj K. Jena, Sudarshan Kumar, Jai K. Kaushik, Ashok K. Mohanty
Summary: The study investigated the differential expression of proteins in in vitro grown buffalo MECs at different time points of differentiation. The results showed a heterogeneous expression of differentially expressed proteins (DEPs) at different time points, with most reaching peak expression at day 12. Bioinformatics analysis indicated the association of DEPs with 24 KEGG pathways, and the identification of new proteins involved in BuMECs differentiation.
Article
Environmental Sciences
Aurelie Lacouture, Yann Breton, Cindy Weidmann, Sarah-Maude Goulet, Lucas Germain, Martin Pelletier, Etienne Audet-Walsh
Summary: This study reveals the impact of estrogens and EDCs on mammary epithelial cell metabolism in monolayer and organoid cultures. It demonstrates that EDCs can alter bioenergetic fluxes and interfere with the regulation of estrogen target genes. The sensitivity of mammary organoids to specific EDCs can also be influenced by past pregnancies. Understanding the metabolic effects of EDCs is important for the development and function of the mammary gland.
ENVIRONMENT INTERNATIONAL
(2023)
Article
Biochemical Research Methods
Oliver Hoecker, Mike Knierman, Jens Meixner, Christian Neusuess
Summary: CE hyphenated to ESI-MS is a well-established technique for analyzing charged analytes, and a new nanoflow sheath liquid CE-MS interface has been developed in this study to improve analysis accuracy and robustness.
Article
Chemistry, Analytical
Luke A. Gallion, Yuli Wang, Angelo Massaro, Ming Yao, Brae Petersen, Quanzheng Zhang, Weigang Huang, Adam J. Carr, Qisheng Zhang, Nancy L. Allbritton
Summary: Capillary electrophoresis with fluorescence detection (CE-F) is a powerful method for measuring enzyme activation in single cells. However, the reporter substrates used in cellular enzymatic assays for CE-F may affect enzyme kinetics. To address this issue, a fix and click method was developed to attach a fluorophore to an enzyme activation reporter prior to analysis by CE-F, allowing for the investigation of sphingolipid signaling in single cells.
ANALYTICAL CHEMISTRY
(2022)
