Article
Chemistry, Analytical
Masaru Taniguchi, Tomiaki Minatani, Hitoshi Miyazaki, Hitoshi Tsuchihashi, Kei Zaitsu
Summary: A highly sensitive quantification method using LC/MS/MS was developed for simultaneous analysis of 12 plant toxins in human serum. The method demonstrated excellent linearity, accuracy, precision, and sensitivity in validation studies. Application of this method to model samples confirmed its practicality for prompt decision-making in accidental poisonings involving toxic plants.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Chemistry, Analytical
Xinliu Gao, Cindy Chen, Dong Geng, Kevin P. Bateman, Shuangping Shi, Eric J. Woolf, Yang Xu
Summary: Microsampling techniques have proven to be advantageous in the pharmaceutical industry as an alternative to traditional serum/plasma sampling due to reduced animal usage and access to otherwise inaccessible samples. While VAMS technology coupled with LC-MS has been extensively used for small molecule drugs, its potential for biological therapeutic development remains to be well-established.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemical Research Methods
Chao Zhong, Baichuan Yang, Weini Huang, Huan Huang, Shasha Zhang, Xi Yan, Qiaomei Lu, Zhuling Chen, Zian Lin
Summary: The immobilized trypsin capillary monolithic column demonstrated faster and more efficient protein digestion compared to free trypsin, with potential applications in high-throughput proteomic analysis.
JOURNAL OF CHROMATOGRAPHY A
(2021)
Article
Chemistry, Analytical
Huai-Hsuan Chiu, Yun-Jung Tsai, Chiao Lo, Ching-Hung Lin, I-Lin Tsai, Ching-Hua Kuo
Summary: This study developed an efficient LC-MS/MS method using an on-bead trypsin digestion procedure to quantify mAb therapeutics. The method showed better sensitivity and reproducibility, making it suitable for real-world analysis of a large number of clinical samples and contributing to personalized mAb treatment.
ANALYTICA CHIMICA ACTA
(2022)
Article
Biochemical Research Methods
Florine Hallez, Audrey Combes, Charlotte Desoubries, Anne Bossee, Valerie Pichon
Summary: A new LC-MS/MS method was developed to detect alkylated peptides resulting from exposure to sulfur mustard. The method accurately identified alkylated sites on proteins and allowed for quantification with satisfying repeatability. It also showed sensitivity towards detecting alkylated peptides resulting from in vitro incubation, suggesting its potential for future analysis of blood samples exposed to sulfur mustard in vivo.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2021)
Article
Biochemical Research Methods
Zuzana Vosahlova-Kadlecova, Martin Gilar, Katarina Molnarova, Petr Kozlik, Kveta Kalikova
Summary: Liquid chromatography coupled with mass spectrometry is widely used for proteomic analysis. In this study, the impact of chromatographic conditions on protein digestion efficiency was investigated. The results showed that online digestion and separation of proteins can be achieved using immobilized enzymatic reactors with optimal activity.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Chemistry, Analytical
Jakub Maculewicz, Piotr Stepnowski, Joanna Dolzonek, Anna Bialk-Bielinska
Summary: Imidazolium ionic liquids (ILs) are increasingly being used, and there is evidence of their presence in the environment. Due to their potential for bio-concentration, it is necessary to monitor the presence of these compounds in organisms from potentially contaminated waters. This study developed a novel analytical procedure for the determination of imidazolium ILs in mussel tissues using a combination of extraction techniques and LC-MS/MS analysis.
Article
Multidisciplinary Sciences
Ronald H. Heisser, Cameron A. Aubin, Ofek Peretz, Nicholas Kincaid, Hyeon Seok An, Elizabeth M. Fisher, Sadaf Sobhani, Perrine Pepiot, Amir D. Gat, Robert F. Shepherd
Summary: This paper presents a new array of individually addressable soft fluidic actuators that operate without electromechanical valves, achieving efficient displacement at small scales using microscale combustion and localized thermal flame quenching. While primarily developed for tactile display technology, this platform microactuator technology has potential applications beyond this field.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Food Science & Technology
Dehua Guo, Xiaojun Deng, Shuqing Gu, Niannian Chen, Xiaomei Zhang, Shuo Wang
Summary: This study achieved the identification and quantification of A1 and A2 type beta-casein proteins at the peptide level for the first time. An automated and online digestion system was used for high throughput digestion of proteins into peptides, which were subsequently separated and analyzed by liquid chromatography-mass spectrometry. Two specific peptides were selected for the identification and quantification of A1 and A2 beta-casein. Online digestion significantly reduced digestion times compared to traditional methods.
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
(2022)
Article
Environmental Sciences
Shaojing Sun, Fan Meng, Hong Qi
Summary: An online solid-phase extraction (SPE) liquid chromatography tandem mass spectrometry method (HPLC-MS/MS) combined with accelerated solvent extraction (ASE) was developed for simultaneous determination of 14 pharmaceuticals in sludge. Ultrapure water with no additives was used as the loading solvent in the online SPE procedures. Low molecular weight targets such as atenolol were difficult to retain on SPE column after acetone was added to the washing solvent. By adding 0.2% formic acid to the mobile phase, the response signal of analytes can be greatly improved. Under the optimized conditions, the recoveries of all the analytes ranged between 75.1 and 112%. The limit of detections ranged from 1.8 to 7.9 ug/kg. The precision of analytical data was determined with relative standard deviation (RSD)<= 4.87%. This method was successfully applied to determine the concentration of pharmaceuticals in sludge.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Biochemical Research Methods
Makoto Ozaki, Motoshi Shimotsuma, Tsunehisa Hirose
Summary: This study found that using a PBr column can better separate highly hydrophilic nicotinamide metabolites with improved retention ability and peak shape compared to the traditional C-18 column. Additionally, it allows for a more sensitive detection of nicotinamide metabolites in tomatoes.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Food Science & Technology
Long-Hui Liang, Yang Yang, Shu Geng, Xi Cheng, Hui-Lan Yu, Chang-Cai Liu, Shi-Lei Liu
Summary: This study presents a rapid and efficient method for the quantification of abrin isoforms in complex matrices using acetonitrile and ultrasound-assisted on-bead trypsin digestion combined with HPLC-MS/MS. It provides valuable insights into the differential identification and quantification of abrin isoforms, improving capabilities for the analysis of biotoxin samples internationally.
Article
Biochemistry & Molecular Biology
Qin Lin, Yujie Qin, Hezhi Sun, Xinru Wang, Mei Yang, Xinzhong Zhang, Li Zhou, Fengjian Luo
Summary: In this study, a detection method for alkylphenol ethoxylates (APEOs) residue in tea was established using solid-phase extraction (SPE) and UPLC-MS/MS. The recoveries of APEOs ranged from 70.3-110.7% with RSD = 16.9%, except for OPEO20 (61.8%) and NPEO20 (62.9%). OPEOs and NPEOs were detected in 50 marketed tea samples with concentrations ranging from 0.057-12.94 μg/kg and 0.30-215.89 μg/kg, respectively. This study provides a theoretical basis for the rational use of APEOs as adjuvants in commercial pesticide production.
Article
Food Science & Technology
Huanyan Liang, Qianyu Hou, Yakui Zhou, Lei Zhang, Meihua Yang, Xiangsheng Zhao
Summary: Mycotoxins can naturally occur in various agricultural products and pose a risk to food safety. An innovative method for analyzing multiple mycotoxins has been developed and validated, providing a useful tool for evaluating contamination levels in Areca catechu and its processed products.
Article
Biochemistry & Molecular Biology
Patricia Duque-Estrada, Kate Hardiman, Astrid Bogebjerg Dam, Nadia Dodge, Margit Dall Aaslyng, Iben Lykke Petersen
Summary: Plant proteins have low nutritional quality due to unbalanced IAA profile and ANFs. Blending pulses with cereals/pseudocereals and applying extrusion processing can improve protein quality. Extrusion reduces TIA and increases protein digestibility, but may cause protein aggregation.