Article
Nanoscience & Nanotechnology
Shayan Valijam, Alireza Salehi, Magnus Andersson
Summary: In this paper, a compact label-free microfluidic lab-on-a-chip device is proposed for the separation of circulating tumor cells (CTCs) and red blood cells (RBCs) at low voltage. The device utilizes a simple electrode arrangement with non-uniform electric fields generated by two arrays of shifted planar electrodes. The device shows high separation efficiency and allows for brightfield imaging of the channel, making it suitable for the early detection of CTCs.
MICROFLUIDICS AND NANOFLUIDICS
(2023)
Article
Biochemistry & Molecular Biology
Emanuele Piccinno, Anna Grazia Monteduro, Francesco Dituri, Silvia Rizzato, Gianluigi Giannelli, Giuseppe Maruccio
Summary: The study validated a miniaturized on-chip platform for real-time, non-destructive cell proliferation assays and drug screening, comparing its effectiveness with the traditional 96-well plate assay. Results showed the new platform's efficacy comparable to the standard method, with advantages in reagent consumption, operator time, and costs, potentially accelerating drug development processes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Biophysics
Vahid Kordzadeh-Kermani, Masoud Madadelahi, Seyed Nezameddin Ashrafizadeh, Lawrence Kulinsky, Sergio O. Martinez-Chapa, Marc J. Madou
Summary: This review introduces the on-board power supply and electrokinetic applications of electrified-LOD (eLOD) devices. By utilizing electrokinetic phenomena, different microfluids can be propelled, separated, and manipulated. While extensive research exists on stationary platforms, the applications on rotating platforms are still underdeveloped.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Engineering, Chemical
Reza Derakhshan, Amirhosein Ghasemi, Reza Moradi, Abas Ramiar
Summary: This paper presents a comprehensive numerical investigation on particle/cell separation in circular microchannels using dielectrophoresis (DEP). The results show that the direction of particle deflection can be controlled by varying the geometrical parameters. By applying DEP, efficient separation of particles and cells based on their size and physical properties is achieved.
ADVANCED POWDER TECHNOLOGY
(2023)
Article
Chemistry, Analytical
Arash Dalili, Hossein Montazerian, Kabilan Sakthivel, Nishat Tasnim, Mina Hoorfar
Summary: This study investigated the factors affecting the displacement of particles in electrophoresis-based microseparators, finding that volumetric throughput has the greatest impact on particle position, with channel length being the most effective factor. The study also successfully demonstrated size-selective sorting or focusing of particles, providing valuable insights into designing microfluidic platforms for selectively separating different cell types in biofluids.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Chemistry, Multidisciplinary
Samaneh Zare Harofte, Madjid Soltani, Saeed Siavashy, Kaamran Raahemifar
Summary: Nowadays, artificial intelligence creates numerous opportunities in the life sciences. Integrating AI with microfluidics can significantly impact various fields of biotechnology.
Article
Chemistry, Physical
Avijit Barik, Xiaoshu Chen, L. James Maher, Arthur E. Warrington, Moses Rodriguez, Sang-Hyun Oh, Nathan J. Wittenberg
Summary: The study demonstrates a gold nanogap electrode platform that utilizes low-voltage dielectrophoresis to rapidly create a linear array of biological particles and combines microfluidic buffer exchange to immobilize particles and introduce protein molecules. The platform can be used to study a variety of biomolecular interactions, making it a potential tool for portable biosensing platforms with improved detection limits in physiological buffers.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2021)
Review
Biochemical Research Methods
Fabian O. Romero-Soto, Maria Polanco-Oliva, Roberto C. Gallo-Villanueva, Sergio O. Martinez-Chapa, Victor H. Perez-Gonzalez
Summary: Cancer is a leading cause of global deaths, with metastasis being the main cause of patient mortality. The scientific community is focused on early cancer detection and prevention of metastatic tumors.
Article
Biochemical Research Methods
Mahsa Jalali, Imman Isaac Hosseini, Tamer AbdelFatah, Laura Montermini, Sebastian Wachsmann Hogiu, Janusz Rak, Sara Mahshid
Summary: EVs shed by cancer cells can provide valuable diagnostic information, but clinical use of liquid biopsy based on EVs is hindered by inefficient testing methods. A nanostructured microfluidic device utilizing SERS has been developed for unambiguous identification of EVs from different tumor cell populations, showing promising potential for broader applications in cancer research.
Review
Biochemical Research Methods
Victor H. Perez-Gonzalez
Summary: Electrokinetically driven insulator-based microfluidic devices offer a way to manipulate particle suspensions, with the concept of insulator-based dielectrophoresis (iDEP) being developed two decades ago. Recent studies challenge the assumption that dielectrophoresis (DEP) is solely responsible for particle trapping in these devices.
Article
Chemistry, Analytical
Abdallah M. Alkhaiyat, Mohamed Badran
Summary: This study proposes a lab-on-chip design that efficiently separates circulating tumor cells (CTCs) from white blood cells (WBCs) and blood platelets (PLTs). Numerical simulations are used to optimize the design parameters, and the effects of applied voltage values and buffer inlet velocity are investigated. The proposed design achieves 100% separation efficiency in simulation and shows potential for early detection of CTCs and personalized cancer treatment.
Article
Chemistry, Multidisciplinary
Matteo Agostini, Francesco Lunardelli, Mariacristina Gagliardi, Alessio Miranda, Leonardo Lamanna, Antonella Giuliana Luminare, Francesca Gambineri, Michele Lai, Mauro Pistello, Marco Cecchini
Summary: Measles is highly infectious, and despite the availability of a vaccine, it remains common in developing countries and sporadic outbreaks occur in developed countries. This study presents a point-of-care biosensing device capable of detecting measles virus in human saliva, with promising results for measles diagnosis and guidance for designing new biosensing systems.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Chemistry, Physical
Cristina Chircov, Alexandra Catalina Birca, Alexandru Mihai Grumezescu, Bogdan Stefan Vasile, Ovidiu Oprea, Adrian Ionut Nicoara, Chih-Hui Yang, Keng-Shiang Huang, Ecaterina Andronescu
Summary: The study introduced a new method for the synthesis of magnetite nanoparticles using a microfluidic lab-on-chip device to achieve controlled properties. It investigated the influence of iron precursor solution concentration and flow on the final properties of the nanomaterials, ultimately achieving uniformity and stability in the synthesized nanoparticles.
Review
Chemistry, Analytical
Ruth Shinar, Joseph Shinar
Summary: Organic electronics (OE) technology has matured in displays and is advancing in solid-state lighting applications. It also shows great potential in (bio)chemical sensing, imaging, in vitro cell monitoring, and other biomedical diagnostics. OE devices, such as organic LEDs, organic and hybrid perovskite-based photodetectors, and organic thin-film transistors, are utilized in these applications. The integration of compact and sensitive OE devices with microfluidic channels and lab-on-a-chip (LOC) structures is very promising.
Article
Chemistry, Analytical
Daphika S. Dkhar, Rohini Kumari, Shweta J. Malode, Nagaraj P. Shetti, Pranjal Chandra
Summary: Lab-on-a-chip (LOC) biosensors have attracted research interest for their potential in personal healthcare and disease diagnostics. This review discusses various fabrication methods and techniques for designing LOC sensing devices, as well as the types of transduction systems that play a crucial role in sensing. The detection of analytes, including small molecules, macromolecules, and cells, is comprehensively reviewed with illustrations and tables.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)