Article
Biochemistry & Molecular Biology
Samantha L. Sholes, Kayarash Karimian, Ariel Gershman, Thomas J. Kelly, Winston Timp, Carol W. Greider
Summary: We developed a method to tag and measure telomere length in yeast using nanopore sequencing. We observed stable chromosome-specific telomere lengths and examined the role of RIF1 and TEL1 in telomere length regulation. The study suggests the influence of Y ' elements on telomere length regulation and provides insights into possible new biological mechanisms.
Article
Biochemistry & Molecular Biology
Tingfang Li, Miaomiao Zhang, Yanjing Li, Xinyu Han, Lu Tang, Tengfei Ma, Xiaotong Zhao, Rui Zhao, Yuwen Wang, Xue Bai, Kai Zhang, Xin Geng, Lei Sui, Xuyang Feng, Qiang Zhang, Yang Zhao, Yang Liu, Jason A. Stewart, Feng Wang
Summary: The human CST complex interacts with the replisome to aid in stalled fork rescue and promotes telomere replication via G-quadruplex (G4) resolution. RECQ4 is identified as a novel CST-interacting partner and can unwind G4 structures in vitro. RECQ4 is crucial for telomere integrity and its depletion results in telomere dysfunction. CST and RECQ4 collaborate to resolve G4-induced replication deficiencies and maintain genomic homeostasis.
Article
Biology
Shuntaro Takahashi, Sudipta Bhowmik, Shinobu Sato, Shigeori Takenaka, Naoki Sugimoto
Summary: The relationship between G4 structures and DNA replication was studied, and it was found that different ligands have different effects on the topology of G4. Some ligands can inhibit the replication of G4 with anti-parallel topology, while others selectively inhibit G4 with anti-parallel and hybrid topology. The experiments suggest that native human telomere G4 is more flexible than the modified sequence and can interact with ligands.
Article
Multidisciplinary Sciences
Sumedha Agashe, Chinnu Rose Joseph, Teresa Anne Clarisse Reyes, Demis Menolfi, Michele Giannattasio, Anja Waizenegger, Barnabas Szakal, Dana Branzei
Summary: The study reveals that Smc5/6 acts together with Top3 within the STR complex to regulate the DNA processing activities of Sgs1 and Top3, maintaining genome structural integrity and mediating DNA replication completion.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
M. A. G. Rabbani, Maiko Luis Tonini, Marjia Afrin, Bibo Li
Summary: This study identifies the telomere protein POLIE as essential for telomere integrity and coordination of telomere G- and C-strand DNA syntheses in Trypanosoma brucei. POLIE suppresses DNA recombination, elongates telomere 3' overhangs, and inhibits telomerase activity. These findings uncover a novel telomere regulation mechanism in T. brucei.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Taylor Takasugi, Peili Gu, Fengshan Liang, Isabelle Staco, Sandy Chang
Summary: Malignant cancers activate telomere maintenance mechanisms through POT1, and loss of POT1 function promotes telomere elongation. Serial transplantation of Pot1b(-/-) sarcomas reveals shortened telomeres in early-generation and hyper-elongated telomeres recognized as damaged DNA in late-generation Pot1b(-/-) cells. RPA-ATR-dependent DNA damage response at telomeres recruits telomerase for hyper-elongation, while POT1b unfolds G-quadruplex and represses the DNA damage response.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Genetics & Heredity
Helene E. B. Geiller, Adam Harvey, Rhiannon E. Jones, Julia W. Grimstead, Kez Cleal, Eric A. Hendrickson, Duncan M. Baird
Summary: Telomerase activity is the main telomere maintenance mechanism in human cancers, but 15% of cancers use a recombination-based mechanism called ALT. Loss of ATRX gene and telomere dysfunction during crisis can initiate the ALT pathway and confer replicative immortality to cells.
Article
Biochemistry & Molecular Biology
Afaf Eladl, Yudai Yamaoki, Shoko Hoshina, Haruka Horinouchi, Keiko Kondo, Shou Waga, Takashi Nagata, Masato Katahira
Summary: The study indicates that the human ORC1 subunit may recognize replication origins through G4 structures, with the external G-tetrad planes of the G4 structures being the primary binding sites for hORC1(413-511).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Cell Biology
Erin Bonnell, Emeline Pasquier, Raymund J. Wellinger
Summary: Telomeres, crucial for maintaining genomic stability, pose challenges for replication due to their repetitive nature and the formation of secondary structures, leading to replication fork stalling. Multiple mechanisms are involved in overcoming these obstacles to ensure efficient telomeric DNA duplication and preserve genome integrity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Anthony Lagnado, Jack Leslie, Marie-Helene Ruchaud-Sparagano, Stella Victorelli, Petra Hirsova, Mikolaj Ogrodnik, Amy L. Collins, Maria Grazia Vizioli, Leena Habiballa, Gabriele Saretzki, Shane A. Evans, Hanna Salmonowicz, Adam Hruby, Daniel Geh, Kevin D. Pavelko, David Dolan, Helen L. Reeves, Sushma Grellscheid, Colin H. Wilson, Sanjay Pandanaboyana, Madison Doolittle, Thomas von Zglinicki, Fiona Oakley, Suchira Gallage, Caroline L. Wilson, Jodie Birch, Bernadette Carroll, James Chapman, Mathias Heikenwalder, Nicola Neretti, Sundeep Khosla, Claudio Akio Masuda, Tamar Tchkonia, James L. Kirkland, Diana Jurk, Derek A. Mann, Joao F. Passos
Summary: The research shows that neutrophils cause oxidative damage to telomeres in non-immune cells, promoting cellular senescence. In aged liver, senescent cells recruit neutrophils, potentially leading to the spread of senescence.
Article
Multidisciplinary Sciences
Hannah Froy, Sarah L. Underwood, Jennifer Dorrens, Luise A. Seeker, Kathryn Watt, Rachael V. Wilbourn, Jill G. Pilkington, Lea Harrington, Josephine M. Pemberton, Daniel H. Nussey
Summary: The study found that in wild Soay sheep, there was no significant association between telomere length and subsequent survival, suggesting that telomere length is not an important marker of biological aging or exposure to environmental stress in this study system. Instead, individual differences in average telomere length were associated with increased lifespan, indicating a genetic basis for this correlation.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Multidisciplinary Sciences
Natthakan Thongon, Feiyang Ma, Andrea Santoni, Matteo Marchesini, Elena Fiorini, Ashley Rose, Vera Adema, Irene Ganan-Gomez, Emma M. Groarke, Fernanda Gutierrez-Rodrigues, Shuaitong Chen, Pamela Lockyer, Sarah Schneider, Carlos Bueso-Ramos, Guillermo Montalban-Bravo, Caleb A. Class, Kelly A. Soltysiak, Matteo Pellegrini, Ergun Sahin, Alison A. Bertuch, Courtney D. DiNardo, Guillermo Garcia-Manero, Neal S. Young, Karen Dwyer, Simona Colla
Summary: The study reveals that telomere attrition leads hematopoietic stem cells towards differentiation into megakaryocytic lineage through upregulated innate immune signaling response, ultimately compromising their self-renewal capabilities and leading to depletion. Targeting the IFI16 signaling axis could potentially prevent functional decline of hematopoietic stem cells under conditions affecting telomere maintenance.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Christina Bauch, Jelle J. Boonekamp, Peter Korsten, Ellis Mulder, Simon Verhulst
Summary: The study investigated the genetic and environmental contributions to variation in telomere length in jackdaws, finding that telomere length variation is primarily influenced by additive genetic effects with a heritability estimated at 0.74 on average. Additionally, they observed low heritable variation for telomere shortening and some impact of year effects on telomere length variation.
Article
Biochemistry & Molecular Biology
Michael Taschner, Stephan Gruber
Summary: This study reconstitutes the ATP-dependent DNA loading process by yeast Smc5/6 rings. Loading strictly requires the Nse5/6 subcomplex to open the kleisin neck gate. The results show that plasmid molecules are topologically entrapped in the kleisin and two SMC subcompartments, but not in the full SMC compartment. This mechanism provides a unifying principle for DNA loading and extrusion.
NATURE STRUCTURAL & MOLECULAR BIOLOGY
(2023)
Review
Genetics & Heredity
Lara Perez-Martinez, Tina Wagner, Brian Luke
Summary: In this mini-review, the authors used a proteomics approach to identify potential RNA regulators associated with telomeric sequences. They found proteins that may positively or negatively regulate TERRA depending on the state of the telomere. These findings expand our understanding of the dynamics of telomere shortening in relation to TERRA and telomere interactors.
FRONTIERS IN GENETICS
(2022)
Review
Biochemistry & Molecular Biology
Yulia Vasianovich, Raymund J. Wellinger
JOURNAL OF MOLECULAR BIOLOGY
(2017)
News Item
Medicine, Research & Experimental
Nancy Laterreur, Raymund J. Wellinger
M S-MEDECINE SCIENCES
(2017)
Article
Biochemistry & Molecular Biology
Laetitia Guintini, Maxime Tremblay, Martin Toussaint, Annie D'Amours, Ralf E. Wellinger, Raymund J. Wellinger, Antonio Conconi
NUCLEIC ACIDS RESEARCH
(2017)
Article
Biochemistry & Molecular Biology
Sofiane Y. Mersaoui, Erin Bonnell, Raymund J. Wellinger
NUCLEIC ACIDS RESEARCH
(2018)
Article
Biochemistry & Molecular Biology
Nancy Laterreur, Bruno Lemieux, Hannah Neumann, Jean-Christophe Berger-Dancause, Daniel Lafontaine, Raymund J. Wellinger
Review
Genetics & Heredity
Sofiane Y. Mersaoui, Raymund J. Wellinger
Article
Multidisciplinary Sciences
Mathilde Gauchier, Sophie Kan, Amandine Barral, Sandrine Sauzet, Eneritz Agirre, Erin Bonnell, Nehme Saksouk, Teresa K. Barth, Satoru Ide, Serge Urbach, Raymund J. Wellinger, Reini F. Luco, Axel Imhof, Jerome Dejardin
Article
Multidisciplinary Sciences
Filip Cervenak, Katarina Jurikova, Hugo Devillers, Binyamin Kaffe, Areej Khatib, Erin Bonnell, Martina Sopkovicova, Raymund J. Wellinger, Jozef Nosek, Yehuda Tzfati, Cecile Neuveglise, Lubomir Tomaska
SCIENTIFIC REPORTS
(2019)
Article
Genetics & Heredity
Emeline Pasquier, Raymund J. Wellinger
EPIGENETICS & CHROMATIN
(2020)
Article
Biology
Yulia Vasianovich, Emmanuel Bajon, Raymund J. Wellinger
Editorial Material
Genetics & Heredity
Muhammad Riaz Khan, Raymund J. Wellinger, Benoit Laurent
Summary: Long noncoding RNA (lncRNA) genes, like protein-coding genes, consist of introns and exons and undergo constitutive and/or alternative splicing after transcription. This review outlines the current understanding of lncRNA splice variants and their functional implications in cell biology.
TRENDS IN GENETICS
(2021)
Review
Cell Biology
Erin Bonnell, Emeline Pasquier, Raymund J. Wellinger
Summary: Telomeres, crucial for maintaining genomic stability, pose challenges for replication due to their repetitive nature and the formation of secondary structures, leading to replication fork stalling. Multiple mechanisms are involved in overcoming these obstacles to ensure efficient telomeric DNA duplication and preserve genome integrity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Genetics & Heredity
Louise Bartle, Yulia Vasianovich, Raymund J. Wellinger
Summary: The maturation process of budding yeast telomerase RNA involves multiple steps and coordination with various protein subunits. This complex pathway ensures that only correctly processed and matured molecules are assembled into active telomerase complexes.
Article
Microbiology
Hannah Neumann, Celia Jeronimo, Jean-Francois Lucier, Emeline Pasquier, Francois Robert, Raymund J. Wellinger, Luc Gaudreau
Summary: The C-terminal region of histone variant H2A.Z in Saccharomyces cerevisiae plays a crucial role in gene regulation by interacting with effector proteins and chaperones, allowing for specific chromatin modifications and gene transcription regulation.
MICROBIOLOGY SPECTRUM
(2023)
Meeting Abstract
Biochemistry & Molecular Biology
Laetitia Guintini, Maxime Tremblay, Martin Toussaint, Annie D'Amours, Ralph E. Wellinger, Raymund J. Wellinger, Antonio Conconi
BIOCHEMISTRY AND CELL BIOLOGY
(2017)
Article
Genetics & Heredity
Marit A. E. van Bueren, Aniek Janssen
Summary: Eukaryotic nuclei rely on multiple repair pathways to accurately repair DNA damage, particularly in chromatin domains enriched for repetitive DNA sequences. Tailored repair mechanisms are necessary to maintain genome stability in these domains.