Article
Chemistry, Physical
Maria Martinez-Negro, Jennifer Oberlaender, Johanna Simon, Volker Mailaender, Svenja Morsbach, Katharina Landfester
Summary: One critical aspect of biomedical material design is controlling plasma protein adsorption to modulate material behavior in biological media. However, there is limited knowledge about the interaction between human plasma and planar surfaces under physiological conditions. To address this challenge, a new methodology combining quartz crystal microbalance with dissipation monitoring (QCM-D) and liquid chromatography coupled with mass spectrometry (LC-MS) has been developed to study protein-surface interactions. This approach provides information on adsorbed protein mass, layer thickness, and proteomic profile.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2023)
Article
Biophysics
Urban Ajdnik, Thomas Luxbacher, Lidija Fras Zemljic
Summary: Controlling protein adsorption on biomaterial surfaces is crucial for successful biointegration. In this study, a combination of electrokinetic measurements and quartz crystal microbalance was used to understand the adsorption phenomena of blood proteins on polysaccharide-based biointerfaces. The results show that the functionalized surfaces have suppressed protein affinity compared to hydrophobic surfaces.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Article
Biotechnology & Applied Microbiology
Yair Kaufman, K. C. Hunt, Gabriel Hale, Matthew McClure, David Latulippe, Madhavi Sivan, Jack Wilson, Rachel Dorin, Yury Agroskin, Marty Siwak, Daniele Gerion
Summary: Membrane fouling by monoclonal antibodies (mAbs) is a challenge in virus-filtration processes, and the differences in fouling between different mAbs have not been fully explained. This study found that low concentration aggregates of mAbs can foul membranes through an adsorptive mechanism driven by hydrophobic forces. The charge of the mAbs plays a secondary role in fouling. These insights can potentially be used to mitigate fouling in other virus-filtration and/or ultrafiltration membranes.
BIOTECHNOLOGY AND BIOENGINEERING
(2023)
Article
Materials Science, Paper & Wood
Arthur Bouchut, Bernard Cathala, Celine Moreau, Michael Lecourt, Michel Petit-Conil, Asja Pettignano, Julien Bernard, Aurelia Charlot, Etienne Fleury
Summary: This study reports a strategy to functionalize cellulosic surfaces through physical adsorption of xyloglucan (XG) and carboxymethyl cellulose (CMC) derivatives bearing allyl or alkyne groups. The derivatives with degrees of substitution ranging from 0.10 to 0.44 are prepared through the opening of the epoxide ring under mild basic aqueous medium. The deposition of these functionalized polysaccharides onto Whatman paper and wood pine fibers shows that the physisorption of the polymer chains is not altered by the extent of the modification or the nature of the substituents.
Book Review
Biochemistry & Molecular Biology
Venkata Appa Reddy Goli, Arun Butreddy
Summary: The development of biosimilar drugs is an important and challenging task. The competition from affordable generics is a major concern for many off-patent biologic drug products. Secondary patents related to formulation delay the availability of biosimilars. Therefore, alternative formulations need to be developed to avoid patent infringement. Innovator companies adopt various strategies to delay biosimilar competition, while biosimilar companies can utilize the innovator's formulation knowledge space for developing alternative formulations.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Biotechnology & Applied Microbiology
Sebastien Viollet, Elise Enouf, Justine Picot, Lena Noel, Simon Huet, Deborah Le Pennec, Thomas Secher, Nathalie Heuze-Vourc'h, Olivier Kitten, Mathieu Cinier
Summary: Nanofitins are small and hyperthermostable protein scaffolds that can be used for topical therapeutics, particularly for pulmonary infectious diseases. They have been developed as highly specific anti-SARS-CoV-2 molecules that show therapeutic efficacy both in vitro and in vivo. The multimeric construction of different Nanofitins seems to involve a conformation switch of the spike trimer, and the tetrameric construction remains stable and active after nebulization.
Review
Pharmacology & Pharmacy
Helen Haixia Wu, Patrick Garidel, Blech Michaela
Summary: The main challenge in developing high-concentration formulations (HCF) for IgG and Ig-based therapeutics is to achieve essential solubility, viscosity, and stability of these molecules. 2-Hydroxypropyl betacyclodextrin (HP-8-CD) has emerged as a promising excipient for HCF development, despite being used in chemical drugs for over thirty years. This review covers the various aspects of HP-8-CD application in pharmaceutical formulation and highlights the challenges and potential future directions in formulation manufacturing for IgG and Ig-based therapeutics.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2021)
Article
Nanoscience & Nanotechnology
Marta Santi, Pabitra Saha, Jacek Janusz Walkowiak, Jens Rubner, Matthias Wessling, Andrij Pich
Summary: A new method to evaluate the protein repellency of microgel coatings was developed using quartz crystal microbalance with dissipation monitoring (QCM-D). This method provides real-time quantitative analysis and high sensitivity, allowing accurate measurement of variations in adsorbed mass.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Engineering, Chemical
Heng Liu, Jiachen Chen, Jiayi Kuang, Yu Yan
Summary: The effects of Co ions and Cr ions on protein adsorption and tribofilm formation were studied using dissipative quartz crystal microbalance (QCM-D) and tribocorrosion experiments. Protein adsorption occurred in two stages regardless of the presence or absence of metal ions, while tribofilm formation occurred after tribocorrosion. Co ions reduced protein adsorption by affecting electrostatic adsorption, whereas Cr ions enhanced the hydrophobic process, resulting in increased adsorption. The size of the tribofilm remained unchanged (about 10-20 µm) after tribocorrosion tests with or without Co ions. However, the addition of Cr ions significantly increased the size of the tribofilm formed by tribocorrosion (about 70 µm), indicating that Cr ions not only increased protein adsorption but also promoted tribofilm growth.
Article
Polymer Science
Jacek Walkowiak, Michael Gradzielski, Stefan Zauscher, Matthias Ballauff
Summary: In this study, a PAA brush was prepared by ATRP of tBA and subsequent acid hydrolysis on gold surfaces of QCM crystals. The interaction between the PAA brush and HSA was studied, showing strong protein adsorption onto the brush and the ability to release HSA molecules by increasing ionic strength. Comparison with calorimetric studies allowed for a thorough analysis of the QCM data based on thermodynamic analysis.
Article
Biochemistry & Molecular Biology
Xiaomin Ma, Xiaosong Sun, Ming Chang, Qingxia Liu, Xianshu Dong, Yuping Fan, Ruxia Chen
Summary: This study examines the interaction between montmorillonite and polyacrylamide (PAM) with different ionic types using QCM-D and MD simulations. The study found that pH decrease led to increased montmorillonite adsorption on the alumina surface. CPAM had the strongest adsorption mass, followed by NPAM, and APAM had the lowest adsorption. CPAM also had the strongest bridging effect, while APAM had a negligible effect. MD simulations showed that the ionicity had a significant influence on the adsorption of polyacrylamides, with the cationic functional group having the strongest attraction.
Article
Biochemistry & Molecular Biology
Agnes Abraham, Flavio Massignan, Gergo Gyulai, Miklos Katona, Nora Taricska, Eva Kiss
Summary: The adhesive properties of amyloid fibers play a crucial role in aggregation processes. Compared to native proteins, amyloid forms create more viscoelastic and hydrophobic adsorption layers, possibly due to their elongated structure and increased hydrophobicity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Nahid Molaei, Omar Bashir Wani, Erin R. Bobicki
Summary: Mitigation of colloid clay particles is crucial in flotation and flocculation processes in mineral processing. Traditional mitigation reagents have negative impacts on the environment and human health, thus biologically derived substances are gaining attention as alternative reagents. This study investigated the adsorption characteristics of three biopolymers on the anisotropic edge and basal plane surfaces of clays, providing insights into their interactions with clay surfaces during selective flotation and flocculation.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Engineering, Chemical
Deniz Karatas, Muhammed Fatih Can, Cengiz Karaguzel, Mehmet S. Celik, Zhenghe Xu
Summary: The adsorption of HTAB and octanol on silica surface was investigated using QCM-D and MD simulations. QCM-D results showed improved adsorption properties with the addition of octanol due to co-adsorption and hydrophobic forces. MD simulations further confirmed the enhanced interaction between HTAB and the silica surface in the presence of octanol.
MINERALS ENGINEERING
(2023)
Article
Biophysics
Timotej Zuntar, Matjaz Licen, Drago Kuzman, Natan Osterman
Summary: This study investigated the reconstitution process of protein films formed by two monoclonal antibodies. The results showed that the reconstitution pace of the films depends on the concentration of the antibodies, which can be predicted quantitatively by a numerical model. Polysorbate 80 was found to inhibit the reconstitution process of the films.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2022)
Article
Chemistry, Physical
Luke A. Clifton, Richard A. Campbell, Federica Sebastiani, Jose Campos-Teran, Juan F. Gonzalez-Martinez, Sebastian Bjorklund, Javier Sotres, Marite Cardenas
ADVANCES IN COLLOID AND INTERFACE SCIENCE
(2020)
Article
Chemistry, Analytical
Alessandra Luchini, Frederik Gronbaek Tidemand, Nicolai Tidemand Johansen, Mario Campana, Javier Sotres, Michael Ploug, Marite Cardenas, Lise Arleth
ANALYTICAL CHEMISTRY
(2020)
Article
Nanoscience & Nanotechnology
Dainius Jakubauskas, Martin Jansen, Jeppe Lyngso, Yuanji Cheng, Jan Skov Pedersen, Marite Cardenas
Summary: Atherosclerosis is a major health challenge today, as total serum cholesterol and lipoprotein concentrations fail to predict risks accurately. Research suggests that LDL particles from individuals with high triglyceride levels exhibit different characteristics compared to those from healthy individuals.
NANOMEDICINE-NANOTECHNOLOGY BIOLOGY AND MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Tobias Palle Holm, Helena Meng-Lund, Jukka Rantanen, Lene Jorgensen, Holger Grohganz
Summary: This study investigated the interactions between different proteins and excipients in freeze-dried products, revealing that the excipients sometimes have a dominant influence on critical quality attributes (CQAs), while in other cases, the CQAs are primarily dependent on the proteins themselves or the combination of both. This research provides insights into the potential impact of excipients on the stability and quality of freeze-dried products, paving the way for the use of novel excipients in the future.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2021)
Article
Chemistry, Physical
Alessandra Luchini, Federica Sebastiani, Frederik Gronbaek Tidemand, Krishna Chaithanya Batchu, Mario Campana, Giovanna Fragneto, Marite Cardenas, Lise Arleth
Summary: Peptide discs, resembling 10 nm discoidal lipid bilayers, can be used as precursors for Supported Lipid Bilayers (SLBs) without the need to alter any experimental variables, allowing for faster formation compared to vesicle fusion. SLBs can be successfully formed from both synthetic and natural lipid mixtures, with traces of 18A peptide at the support surface having no effect on the SLB structure.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Federica Sebastiani, Marianna Yanez Arteta, Michael Lerche, Lionel Porcar, Christian Lang, Ryan A. Bragg, Charles S. Elmore, Venkata R. Krishnamurthy, Robert A. Russell, Tamim Darwish, Harald Pichler, Sarah Waldie, Martine Moulin, Michael Haertlein, V. Trevor Forsyth, Lennart Lindfors, Marite Cardenas
Summary: This study investigated the structure and lipid distribution of LNPs in the presence of ApoE using small-angle neutron scattering. The binding of ApoE was found to cause a redistribution of lipids at the shell and core of LNPs, impacting their internal structure and mRNA release. The rearrangement of LNP components upon ApoE incubation may have implications for LNP endosomal escape.
Article
Chemistry, Multidisciplinary
Sarah Waldie, Federica Sebastiani, Martine Moulin, Rita Del Giudice, Nicolo Paracini, Felix Roosen-Runge, Yuri Gerelli, Sylvain Prevost, John C. Voss, Tamim A. Darwish, Nageshwar Yepuri, Harald Pichler, Selma Maric, V. Trevor Forsyth, Michael Haertlein, Marite Cardenas
Summary: The study investigated the interaction between ApoE variants and model membranes, the formation and structural characteristics of rHDL, and their lipid exchange ability. The results showed that different ApoE structures are sensitive to lipid unsaturation, and ApoE-rHDL mainly exchange saturated lipids.
FRONTIERS IN CHEMISTRY
(2021)
Article
Electrochemistry
Palraj Kalimuthu, Juan F. Gonzalez-Martinez, Dainius Jakubauskas, Marite Cardenas, Tautgirdas Ruzgas, Javier Sotres
Summary: This study presents a prototype of a passive radio frequency sensor for wireless detection of bacteria, based on the bacterial-induced degradation of a gelatin-caprylic acid composite film. By wirelessly monitoring the decrease in resistivity of the film, the presence of bacteria can be detected in real time, providing a novel detection method for biomedical, food safety, and environmental applications.
ELECTROCHIMICA ACTA
(2021)
Article
Chemistry, Physical
Yubexi Correa, Sarah Waldie, Michel Thepaut, Samantha Micciulla, Martine Moulin, Franck Fieschi, Harald Pichler, V. Trevor Forsyth, Michael Haertlein, Marite Cardenas
Summary: Cholesterol levels and lipid disorders are correlated with Covid-19 severity. The SARS-CoV-2 spike (S) protein interferes with lipoprotein function, particularly dependent on cholesterol.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Physical
Federica Sebastiani, Marianna Yanez Arteta, Lennart Lindfors, Marite Cardenas
Summary: A time and cost-effective method was proposed to screen LNP formulations and select the most promising candidates for in vitro and in vivo studies. Two different approaches were explored to investigate the binding affinity between LNPs and serum proteins using sensor functionalisation.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Chemistry, Physical
Alessandra Luchini, Frederik Gronbaek Tidemand, Raul Araya-Secchi, Mario Campana, Marite Cardenas, Lise Arleth
Summary: This study provides a complete structural model of tissue factor and its complex with factor VIIa in a lipid bilayer using experimental and computational methods. The study reveals that phosphatidylserine lipids play a role in promoting the binding of tissue factor and factor VIIa, which is crucial for blood coagulation.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2022)
Article
Nanoscience & Nanotechnology
Nicolo Paracini, Philipp Gutfreund, Rebecca Welbourn, Juan Francisco Gonzalez-Martinez, Kexin Zhu, Yansong Miao, Nageshwar Yepuri, Tamim A. . Darwish, Christopher Garvey, Sarah Waldie, Johan Larsson, Max Wolff, Marite Cardenas
Summary: Arrays of nanoparticle-supported lipid bilayers (nanoSLB) are lipid-coated nanopatterned interfaces that provide a platform to study curved model biological membranes using surface-sensitive techniques. We investigated the structure of lipid bilayers formed on nanoparticle arrays through vesicle fusion using scattering techniques and direct imaging. The presence of both flat and curved supported lipid bilayers on the same substrate, combined with the accuracy of grazing incidence neutron scattering, offers a promising approach to studying curvature-dependent membrane phenomena.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Biochemistry & Molecular Biology
Rita Del Giudice, Nicolo Paracini, Tomas Laursen, Clement Blanchet, Felix Roosen-Runge, Marite Cardenas
Summary: This study expands the toolkit of lipid and detergent combinations that allow the formation of stable bicelles. Systematic sample characterization using various techniques provides a set of conditions under which bicelles can be successfully formed.
Article
Chemistry, Multidisciplinary
Alessandra Luchini, Frederik Gronbaek Tidemand, Nicolai Tidemand Johansen, Federica Sebastiani, Giacomo Corucci, Giovanna Fragneto, Marite Cardenas, Lise Arleth
Summary: This study introduced a new method for producing SLBs with incorporated membrane proteins using a new variant of the 18A peptide, d18A. The dark peptide d18A allows for direct quantification of membrane protein concentration in the peptide discs, improving sample preparation efficiency. Results showed successful formation of SLBs with membrane protein VAMP2 using d18A-prepared peptide discs.
NANOSCALE ADVANCES
(2022)
Article
Multidisciplinary Sciences
Alexei Vorobiev, Nicolo Paracini, Marite Cardenas, Max Wolff
Summary: Grazing incidence neutron small angle scattering using a fan shaped incident beam allows for reduced counting times, resolving the structure factor of iron inclusions in aluminum oxide and the ordering of silica particles on a silicon substrate. Different packing behaviors were observed for 50 nm and 200 nm silica spheres, with particle shape extraction from the form factor and dense packing on a free water surface reported. The method named pi-GISANS provides lateral information while averaging in-depth structure.
SCIENTIFIC REPORTS
(2021)