Article
Biochemical Research Methods
Xiaoyao Miao, Shuo Li, Bingkun Xiao, Jianyun Yang, Rongqing Huang
Summary: The study demonstrated that Danggui Buxue Tang could enhance endurance and reduce immobility in rats, as well as regulate various metabolic pathways to improve chronic fatigue syndrome.
BIOMEDICAL CHROMATOGRAPHY
(2022)
Article
Plant Sciences
Marko Bertic, Hilke Schroeder, Birgit Kersten, Matthias Fladung, Franziska Orgel, Franz Buegger, Joerg-Peter Schnitzler, Andrea Ghirardo
Summary: This study conducted in oak forests in Germany demonstrates that oak trees resistant to herbivory allocate resources towards constitutive chemical defense enriched with polyphenolic compounds, while susceptible oaks allocate resources towards growth-promoting substances. The discovery of metabolic biomarkers and developed predictive model sheds light on the susceptibility of Quercus robur to herbivore attack and contributes to forest management for the preservation of oak forests in Europe.
Article
Biochemistry & Molecular Biology
Eirini Pegiou, Qingrui Zhu, Paraskevas Pegios, Ric C. H. De Vos, Roland Mumm, Robert D. Hall
Summary: Green and white asparagus, though harvested from the same plant, show distinct morphological and chemical differences due to different cultivation methods. Green asparagus is rich in C-5 and C-8 alcohols and aldehydes, while white asparagus contains more benzenoids and monoterpenes. Spatial metabolomics revealed that volatile compounds with positive aroma attributes are more abundant in the upper parts of the spears, decreasing towards the base.
Article
Plant Sciences
Yongxia Guan, Jianwei Fan, Chenghong Sun, Yiqing Yang, Jianye Quan, Guimin Zhang, Na Guo
Summary: The study found that GRR affects the synthesis of ginsenosides in 20-year-old MCG, resulting in lower concentrations of key ginsenosides and differences in elemental composition.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Food Science & Technology
Chuan Ma, Beibei Ma, Jianke Li, Yu Fang
Summary: This study investigated the quality changes of royal jelly produced by migratory honeybees at different floral periods. Metabolomics and proteomics analysis revealed variations in the metabolic and proteomic profiles of royal jelly. Minor sugars and a major royal jelly protein were identified as the main components contributing to the altered profiles. The water content, crude protein, and a specific acid content met the international standards regardless of floral periods. Moreover, changes in antioxidants resulted in significant differences in the antioxidant activity of royal jelly.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Food Science & Technology
Yuming Wei, Tiehan Li, Shanshan Xu, Tiancheng Ni, Wei-Wei Deng, Jingming Ning
Summary: Yellowing is the key process in producing yellow tea, but its impact on tea quality and chemical composition is not fully understood. This study used electronic eye, tongue, and metabolomics to investigate the dynamic changes in tea leaves during yellowing. The early stages of yellowing showed increased levels of compounds related to umami and sweetness, while the later stages resulted in darker color and decreased richness in tea infusion, accompanied by significant changes in levels of various compounds.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Pharmacology & Pharmacy
Wei Chen, Jiayun Xin, Xintong Wei, Qianqian Ding, Yunheng Shen, Xike Xu, Yanping Wei, Yanhui Lv, Jie Wang, Zhanhong Li, Weidong Zhang, Xianpeng Zu
Summary: The combination of Danggui Buxue Decoction (DBD) and icaritin (ICT), a derivative herbal formula called MDBD, has been proven to effectively alleviate leukopenia caused by radiation. It works by improving the activity of bone marrow nuclear cells, reducing bone marrow damage, modulating spleen index, and regulating hematopoietic factors.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Ying Guo, Yating Zhang, Ya Hou, Pengmei Guo, Xiaobo Wang, Sanyin Zhang, Peng Yang
Summary: Danggui Buxue decoction, containing Angelicae Sinensis Radix and Astragali Radix, can relax the rat MCA with higher efficacy on precontracted vessels. Angelicae Sinensis Radix shows superior vasodilator effects on KCl-contracted MCA compared to other components. DBD exhibits stronger vasodilator effects on MCA at various concentrations.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Food Science & Technology
Min Jeong Kang, Keup-Rae Kim, Keono Kim, Aria G. Morrill, Chuleui Jung, Sukjun Sun, Dong-Hee Lee, Joon Hyuk Suh, Jeehye Sung
Summary: This study used metabolomics and sensory analysis to investigate the relationship between chemical profile and sensory quality of honey. The results showed that sweetness had a positive impact on consumer acceptance, while sourness, astringency, and bitterness had a negative impact. Consumers preferred honey with floral notes, but disliked those with off-flavors. The familiarity of flavor was strongly correlated with consumer acceptance, indicating the importance of balancing volatiles and non-volatiles for honey flavor quality.
FOOD RESEARCH INTERNATIONAL
(2023)
Article
Plant Sciences
Marta Bertola, Laura Righetti, Laura Gazza, Andrea Ferrarini, Flavio Fornasier, Martina Cirlini, Veronica Lolli, Gianni Galaverna, Giovanna Visioli
Summary: Perennial grains, with their extensive root system and permanent soil cover, provide various ecosystem services compared to annual grains. However, the evolution and diversification of the rhizosphere and its ecological functions over time are not well understood. In this study, a suite of -OMICSs was used to compare the rhizosphere environment of four perennial wheat lines at different growth stages. The results showed that wheat perenniality played a greater role in shaping the rhizobiome composition, biomass, diversity, and activity than plant genotypes. The continuous supply of sugars in the rhizosphere created a favorable environment for microbial growth, resulting in higher microbial biomass and enzymatic activity. Changes in the rhizosphere metabolome and lipidome over the years led to the coexistence of more diverse microbial taxa and increased plant tolerance to stresses.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Mai Mehanna, Caitrin W. McDonough, Steven M. Smith, Yan Gong, John G. Gums, Arlene B. Chapman, Julie A. Johnson, Lauren McIntyre, Rhonda M. Cooper-DeHoff
Summary: This study identified metabolic signatures associated with baseline PRA and their linkages with the effects of thiazides and beta-blockers in regulating blood pressure, providing insights into the mechanisms underlying BP response.
Article
Agriculture, Multidisciplinary
Paola Bambina, Alberto Spinella, Giuseppe Lo Papa, Delia Francesca Chillura Martino, Paolo Lo Meo, Onofrio Corona, Luciano Cinquanta, Pellegrino Conte
Summary: The soil effect on the micro-component composition of Nero d'Avola wines obtained from different locations was investigated using 1H NMR-based metabolomics. The study found that the differences among wines were not only due to the concentrations of various analytes but also to the characteristics of the H-bond network. Furthermore, the H-bond network was found to be related to the soil properties.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Jin Xu, Rebecca Green, Min Kim, Jodie Lord, Amera Ebshiana, Sarah Westwood, Alison L. Baird, Alejo J. Nevado-Holgado, Liu Shi, Abdul Hye, Stuart G. Snowden, Isabelle Bos, Stephanie J. B. Vos, Rik Vandenberghe, Charlotte E. Teunissen, Mara Ten Kate, Philip Scheltens, Silvy Gabel, Karen Meersmans, Olivier Blin, Jill Richardson, Ellen Elisa De Roeck, Sebastiaan Engelborghs, Kristel Sleegers, Regis Bordet, Lorena Rami, Petronella Kettunen, Magda Tsolaki, Frans R. J. Verhey, Daniel Alcolea, Alberto Lleo, Gwendoline Peyratout, Mikel Tainta, Peter Johannsen, Yvonne Freund-Levi, Lutz Froelich, Valerija Dobricic, Giovanni B. Frisoni, Jose Luis Molinuevo, Anders Wallin, Julius Popp, Pablo Martinez-Lage, Lars Bertram, Kaj Blennow, Henrik Zetterberg, Johannes Streffer, Pieter Jelle Visser, Simon Lovestone, Petroula Proitsi, Cristina Legido-Quigley
Summary: Physiological differences between males and females play a role in the development of Alzheimer's Disease, with distinct metabolic pathways observed in each gender. Specific metabolites related to neurotransmission and inflammation were found to be associated with AD endophenotypes in females, potentially paving the way for personalized treatment.
Article
Medicine, Research & Experimental
Hui Li, Pei Liang Dong, Xiao Jin Yu, Qing Na Li, Jia Qi Liu, Cai Yan Liu, Hua Han
Summary: The aim of this study was to investigate the mechanism and effects of Danggui-kushen herb pair (DKHP) in treating ischemic heart disease (IHD) compared to single drugs. The results showed that DKHP performed better in ameliorating myocardial injury, which was attributed to the inhibition of NF-kappa B signaling and activation of HIF-1 alpha signaling pathways.
BIOMEDICINE & PHARMACOTHERAPY
(2022)
Article
Fisheries
Jun -Ming Zhang, Bing Fu, Yi-chao Li, Jin-Hui Sun, Jun Xie, Guang-Jun Wang, Jing-Jing Tian, Gen Kaneko, Er-Meng Yu
Summary: This study explored the effects of nitrite and nitrate on the growth performance, nutrition composition, and muscle flavor of grass carp. Nitrite and nitrate treatments reduced the growth performance and nutrient content in the muscle, while increasing water content. Nitrite treatment produced more differential metabolites and mainly affected amino acid metabolism and protein digestion pathways.
Article
Biochemistry & Molecular Biology
Zheng-Zheng Zhang, Dong Tian, Ji-Ning Gao, Xue-Mei Qin, Zhen-Yu Li
ZEITSCHRIFT FUR NATURFORSCHUNG SECTION C-A JOURNAL OF BIOSCIENCES
(2015)
Article
Chemistry, Analytical
Zheng-Zheng Zhang, Ma-Li Fan, Xia Hao, Xue-Mei Qin, Zhen-Yu Li
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2016)
Article
Multidisciplinary Sciences
Laura M. de Jong, Zhengzheng Zhang, Yvette den Hartog, Timothy J. P. Sijsenaar, Renata Martins Cardoso, Martijn L. Manson, Thomas Hankemeier, Peter W. Lindenburg, Daniela C. F. Salvatori, Miranda Van Eck, Menno Hoekstra
Summary: Pharmacological inhibition of PRMT3 can reduce hepatic steatosis and plasma triglyceride levels, but may also induce pruritus in the presence of a high-fat diet.
SCIENTIFIC REPORTS
(2022)
Article
Immunology
Zhengzheng Zhang, Kerina Duri, Kevin L. W. Duisters, Johannes C. Schoeman, Panashe Chandiwana, Peter Lindenburg, Julia Jaeger, Susanne Ziegler, Marcus Altfeld, Isabelle Kohler, Amy Harms, Felicity Z. Gumbo, Thomas Hankemeier, Madeleine J. Bunders
Summary: This study investigated immune-metabolic dysregulation in children born to women living with HIV. Through longitudinal analysis of immune-metabolomic profiles, it was found that HIV-exposed-uninfected children with long combinational antiretroviral therapy (cART) exposure had higher levels of methionine-sulfone, associated with oxidative stress, compared to HIV-unexposed children. Increased methionine-sulfone levels were associated with impaired infant growth. These findings highlight the importance of addressing immune-metabolic dysregulation in children born to women living with HIV.
Article
Biochemical Research Methods
Zhengzheng Zhang, Madhulika Singh, Alida Kindt, Agnieszka B. Wegrzyn, Mackenzie J. Pearson, Ahmed Ali, Amy C. Harms, Paul Baker, Thomas Hankemeier
Summary: The importance of lipidomics research in understanding various diseases, including metabolism, cancer, and the recent COVID-19 pandemic, has led to the development of a targeted HILIC-MS/MS method that allows for comprehensive analysis of lipid species. This method overcomes the challenges posed by the diverse structures and properties of lipids in biological systems, and provides accurate quantitation of lipid concentrations at the fatty acyl chain level. The applicability of this method has been demonstrated through the discovery of differential lipid features related to COVID-19 severity, highlighting its potential for future investigations of the lipidome in different disease contexts.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
