Review
Biochemistry & Molecular Biology
Yago Gecy de Sousa Ne, Weslley Ferreira Lima, Paulo Fernando Santos Mendes, Daiane Claydes Baia-da-Silva, Leonardo Oliveira Bittencourt, Priscila Cunha Nascimento, Renata Duarte de Souza-Rodrigues, Luiz Renato Paranhos, Paulo Antonio Martins-Junior, Rafael Rodrigues Lima
Summary: This study aimed to analyze the research trends on salivary oxidative stress associated with dental caries and to perform bibliometric approaches for existing publications on this association. The findings show that India and Asia have the highest number of publications on this topic, non-stimulated total saliva is the most commonly collected sample, and total antioxidant capacity is the most frequently evaluated parameter. Cross-sectional studies are the most common study design, and articles published from 2017 to 2022 are the most frequent.
Article
Biochemistry & Molecular Biology
Bahareh Nazemi Salman, Shayan Darvish, Ancuta Goriuc, Saeideh Mazloomzadeh, Maryam Hossein Poor Tehrani, Ionut Luchian
Summary: Salivary markers of oxidative stress are associated with clinical disease indices such as gingival bleeding and dental caries. The total antioxidant capacity (TAC) increased in patients with more gingival bleeding and decreased in adolescents with caries. Antioxidants could potentially be used as a preventive and therapeutic measure against oral diseases in the future.
Review
Biochemistry & Molecular Biology
Yago Gecy de Sousa Ne, Deborah Ribeiro Frazao, Leonardo Oliveira Bittencourt, Nathalia Carolina Fernandes Fagundes, Guido Maranon-Vasquez, Maria Elena Crespo-Lopez, Lucianne Cople Maia, Rafael Rodrigues Lima
Summary: This systematic review aimed to assess the association between dental caries and oxidative salivary stress. The studies found an imbalance of antioxidant and pro-oxidant parameters in individuals with dental caries, indicating a disbalance of antioxidants and pro-oxidants. However, the evidence level is very low.
Article
Dentistry, Oral Surgery & Medicine
Ayse Toraman, Ebru Saglam, Levent Savran, Mehmet Saglam, Serhat Koseoglu
Summary: Salivary levels of NLRC4 and IL-1 beta were significantly higher in patients with gingivitis and periodontitis compared to the healthy group. Positive correlations were observed between salivary levels of NLRC4 and IL-1 beta and clinical parameters. This suggests that salivary NLRC4 may serve as a potential marker for detecting the presence and/or severity of periodontal disease.
Article
Dentistry, Oral Surgery & Medicine
Oscar Musalem-Dominguez, Jose Maria Montiel-Company, Veronica Ausina-Marquez, Jose Manuel Morales-Tatay, Jose Manuel Almerich-Silla
Summary: This study identified metabolomic differences in saliva between healthy children and children with active carious lesions, and developed a predictive model based on the salivary metabolomic profile. The results showed differences in metabolites, particularly saccharides and amino acids, between the two groups, suggesting their association with caries risk. These findings contribute to the research of salivary biomarkers, caries risk assessment, and personalized prevention strategies.
JOURNAL OF DENTISTRY
(2023)
Article
Dentistry, Oral Surgery & Medicine
R. Raksakmanut, P. Thanyasrisung, S. Sritangsirikul, K. Kitsahawong, A. L. Seminario, W. Pitiphat, O. Matangkasombut
Summary: Dental caries, the most common chronic disease in children, has negative effects on their health, development, and well-being. Early preventive interventions are crucial, but there is still a lack of accurate caries risk predictors for infants before caries onset.
JOURNAL OF DENTAL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Raphael Charchar Campos Alves, Railson Oliveira Ferreira, Deborah Ribeiro Frazao, Yago Gecy de Souza Ne, Paulo Fernando Santos Mendes, Guido Maranon-Vasquez, Luiz Fernando Freire Royes, Nathalia Carolina Fernandes Fagundes, Lucianne Cople Maia, Rafael Rodrigues Lima
Summary: This systematic review evaluated the effects of physical exercise on oxidative stress in saliva. The findings suggest that exercise may increase oxidative stress and antioxidant activity in saliva, but there is heterogeneity and inconsistency among studies.
Article
Dentistry, Oral Surgery & Medicine
Jordana Resende Martins, Beatriz Diaz-Fabregat, Wilmer Ramirez-Carmona, Douglas Roberto Monteiro, Juliano Pelim Pessan, Cristina Antoniali
Summary: There is a relationship between salivary biomarkers of oxidative stress and dental caries in children. The levels of oxidative stress biomarkers and salivary parameters are altered in the saliva of children with dental caries.
ARCHIVES OF ORAL BIOLOGY
(2022)
Article
Dentistry, Oral Surgery & Medicine
Y. Kurushima, P. M. Wells, R. C. E. Bowyer, N. Zoheir, S. Doran, J. P. Richardson, D. D. Sprockett, D. A. Relman, C. J. Steves, L. Nibali
Summary: Limited evidence is available on how host genetic variants affect microbiota composition. This study examines the associations between candidate host genetic variants and microbial composition in saliva and gut. The findings suggest that certain genetic variants are associated with microbial diversity and composition, and periodontal status may drive variations in the microbiota.
JOURNAL OF DENTAL RESEARCH
(2023)
Article
Dentistry, Oral Surgery & Medicine
Sami Abdoh Assiri, Omar Abd El Sadek El Meligy, Ibtesam Omar Alzain, Nada Othman Bamashmous
Summary: The study found that children with type 1 diabetes in Saudi Arabia had lower caries experience in primary teeth compared to healthy children, and also had lower buffering capacity in saliva.
JOURNAL OF DENTAL SCIENCES
(2022)
Article
Pediatrics
Sara Ayid Alghamdi, Aziza Aljohar, Basim Almulhim, Abdullah Alassaf, Smita Singh Bhardwaj, Julie Toby Thomas, Abdullah Almalki, Areej Owaid Aljuaid, Sreekanth Kumar Mallineni
Summary: The study aimed to investigate the association between varying body mass index (BMI) and oral health status among children aged 5-14 years, as well as the correlation between salivary levels of 1,5-AG, BMI, dental caries, and periodontal disease. The results showed that obese children had higher scores in terms of dental caries and periodontal disease. Therefore, promoting preventive oral health regimes, health awareness campaigns, and nutritional educational programs among children is crucial.
Article
Biochemistry & Molecular Biology
Seonghye Kim, Yuri Song, Seyeon Kim, Siyeong Kim, Heesam Na, Sujin Lee, Jin Chung, Suhkmann Kim
Summary: Several studies have shown that nuclear magnetic resonance (NMR) metabolic profiles can differentiate patients with caries from healthy individuals. This study aimed to identify a salivary metabolite biomarker panel for early childhood caries (ECC). Saliva samples from children with and without caries were analyzed using NMR spectroscopy, and specific metabolites were identified that could detect ECC. An optimal biomarker panel comprising formate, lactate, proline, and glycine was developed, which showed improved diagnostic performance for ECC compared to a single metabolite.
Article
Pediatrics
Cansu Ozsin-Ozler, Onurcem Duruel, Asli Pinar, Begum Ozbek, Ismail Yaz, Emel Tugba Ataman-Duruel, Meryem Uzamis-Tekcicek, Ebru Gunes-Yalcin, Deniz Dogru-Ersoz, Nural Kiper, Ilhan Tezcan, Ezel Berker
Summary: The purpose of this study was to evaluate the caries status of children and adolescents with cystic fibrosis (CF) by comparing various biochemical markers, secretory immunoglobulin A (sIgA), and antimicrobial peptides in saliva. The results showed that CF patients had lower caries in permanent teeth and filling in primary teeth, and an altered profile of salivary biomarkers, particularly HNB1 and HNP1. Therefore, it is crucial to regularly monitor dental health in CF patients during childhood.
PEDIATRIC PULMONOLOGY
(2022)
Article
Immunology
Tatjana Kanjevac, Ervin Taso, Vladimir Stefanovic, Aleksandra Petkovic-Curcin, Gordana Supic, Dejan Markovic, Mirjana Djukic, Boris Djuran, Danilo Vojvodic, Anton Sculean, Mia Rakic
Summary: The study investigated the effects of caries and restorative materials on inflammatory and oxidative markers in gingival crevicular fluid. Caries affected teeth showed altered levels of inflammatory markers, while different restorative materials exhibited distinctive material-specific patterning.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Environmental Sciences
Jae-Hwan Kim, Mi-Ah Kim, Yong Kwon Chae, Ok Hyung Nam
Summary: This study investigated the salivary characteristics and daily living patterns in Korean children aged 12 years, and evaluated their relationships according to the significant caries (SiC) index. Differences in salivary Mutans streptococci (MS) levels were significant between low caries-affected and high caries-affected groups. The prevalence of consuming sugar-associated energy sources between meals was significantly higher in the high caries-affected group.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2021)
Review
Immunology
Michal Pastorek, Martin Dubrava, Peter Celec
Summary: Despite ongoing vaccination, COVID-19 remains a global healthcare problem due to the lack of effective targeted therapy. Severe cases of COVID-19 involve uncontrolled activation of the innate immune system, resulting in cytokine deregulation, release of damage-associated molecular patterns, and a high occurrence of thrombotic events. These pathomechanisms are associated with neutrophil function and dysfunction, specifically the excessive formation of neutrophil extracellular traps (NETs). This review aims to summarize potential inducers of NETs formation in severe COVID-19 and discuss potential treatment options targeting NETs formation or removal.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Oncology
Jonathan L. Hecht, Monika Janikova, Reeham Choudhury, Fong Liu, Giacomo Canesin, Lubica Janovicova, Eva Csizmadia, Elisa M. Jorgensen, Katharine M. Esselen, Peter Celec, Kenneth D. Swanson, Barbara Wegiel
Summary: This study investigates the link between endometriosis and ovarian clear cell cancer (OCCC) and proposes that altered heme metabolism and the presence of oxidative stress in the immune niche may drive OCCC development. The study also highlights the role of macrophages in this process and identifies elevated levels of HO-1 as a potential biomarker for inflammation.
Article
Biochemistry & Molecular Biology
L'ubica Janovicova, Katarina Kmet'ova, Nikola Pribulova, Jakub Janko, Barbora Gromova, Roman Gardlik, Peter Celec
Summary: This study investigated the impact of high endogenous DNase activity on an animal model of acute liver failure. It was found that mice with higher DNase activity had lower mortality after 48 hours. The study also suggested that vesicles or DNA-binding proteins may play a role in protecting ecDNA from nucleases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Nutrition & Dietetics
Veronika Kovacova, Radoslav Omelka, Vladimira Mondockova, Piotr Londzin, Jozef Conka, Veronika Meliskova, Joanna Folwarczna, Peter Celec, Monika Martiniakova
Summary: Short-term animal experiments and association studies have suggested that cola intake may have a negative impact on bone mineral density (BMD). This study investigated the effects of long-term cola consumption on femoral bone microstructure in adult mice. The results showed that cola intake did not significantly affect trabecular and cortical bone parameters, although slight deterioration was observed. Cola consumption also slightly worsened bone mechanical properties, but it was not statistically significant. Long-term cola intake did not cause evident pathological changes, possibly due to a balanced diet and physical activity.
Article
Multidisciplinary Sciences
Lucia Mihalovicova, Veronika Kunstekova, David Milacek, Jakub Janko, Michal Pastorek, Barbora Konecna, Radana Gurecka, Zuzana Rausova, Olga Ulicna, Peter Celec, Katarina Sebekova
Summary: The study investigates the impact of maternal gestational diabetes (GDM) on the metabolic status of offspring. GDM was induced in CD1 mice consuming a fast-food diet (FFD) before mating. Offspring from dams consuming standard chow or FFD served as controls. Results showed that male GDM offspring exhibited growth retardation and lower kidney weight. GDM offspring, regardless of sex, had the lowest IL-1 alpha levels, while other inflammatory markers showed inconsistent changes. Offspring from dams consuming FFD had higher liver triacylglycerols content.
SCIENTIFIC REPORTS
(2023)
Article
Medicine, General & Internal
Maria Fussiova, Peter Svec, Julia Horakova, Petr Sedlacek, Peter Rohon, Peter Celec, Ivana Bod'ova, Jaroslava Adamcakova, Tomas Sykora, Veronika Dobsinska, Miroslava Pozdechova, Dominika Doczyova, Santia Vargova, Alexandra Kolenova
Summary: This study compared the course of HVOD in children before and after the implementation of new EBMT criteria. The use of EBMT criteria resulted in a higher recognition rate of anicteric disease, lower serum bilirubin levels at diagnosis, shorter treatment duration, decreased mortality, and shorter hospitalization.
JOURNAL OF CLINICAL MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Kristina Licha, Michal Pastorek, Gabriela Repiska, Peter Celec, Barbora Konecna
Summary: The study aimed to investigate the presence of ecDNA in sEVs isolated from fresh human plasma by ultracentrifugation and density gradient. It was found that approximately 60-75% of DNA is on the surface of sEVs, while a part of the DNA is localized inside the sEVs. Furthermore, both nuclear and mitochondrial DNA were present in plasma EVs.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemical Research Methods
Lubica Janovicova, Katarina Kmetova, Lubomira Tothova, Barbora Vlkova, Peter Celec
Summary: Urinary DNA is studied as a non-invasive marker to monitor kidney health and urinary tract tumors. The concentration of urinary DNA, especially mitochondrial DNA, reflects kidney damage in renal diseases. Sample processing affects urinary DNA concentrations, with centrifugation at 1600 g decreasing DNA concentration by 66% in high DNA samples. Additional centrifugation at 16000 g does not significantly decrease DNA concentration in the supernatant. Deoxyribonuclease treatment reduces total and nuclear DNA, but most urinary mitochondrial DNA is protected. Understanding the biology of urinary DNA is important for clinical interpretation.
MOLECULAR AND CELLULAR PROBES
(2023)
Article
Physics, Applied
Barbora Gromova, Barbora Konecna, Maria Suchonova, Slavomir Pasztor, Peter Celec, Lubomira Tothova, Michal Lichvar, Jaroslav Budis, Jan Radvanszky, Diana Rusnakova, Tomas Szemes, Zdenko Machala, Roman Gardlik
Summary: This study analyzed the impact of plasma-activated water (PAW) on oxidative status and gut microbiota diversity in healthy mice and mice with inflammatory bowel disease (IBD). The results showed that PAW increased oxidative stress markers in the colon tissue, especially in IBD mice. Interestingly, PAW increased microbial diversity in a healthy gut but decreased it in an inflamed gut. The findings suggest that PAW may not have therapeutic potential in treating IBD.
PLASMA PROCESSES AND POLYMERS
(2023)
Article
Pediatrics
Katarina Krivosikova, Nadja Supcikova, Alexandra Gaal Kovalcikova, Jakub Janko, Michal Pastorek, Peter Celec, Ludmila Podracka, Lubomira Tothova
Summary: This study aimed to investigate the role of NET-associated markers in children with UTI and the role of NETs formation in a mouse model of UTI. The results showed that children with UTI had significantly higher urinary NETs markers including ecDNA, MPO, and cathelicidin, which were positively correlated with leukocyturia. The experimental model also revealed that mice with diminished NETs formation had a higher bacterial load in the bladder and kidneys, suggesting that NETs may play a direct role in preventing pyelonephritis and other UTI complications.
FRONTIERS IN PEDIATRICS
(2023)
Article
Oncology
Peter Lesko, Barbora Vlkova, Katarina Kalavska, Valentina De Angelis, Vera Novotna, Jana Obertova, Zuzana Orszaghova, Patrik Palacka, Katarina Rejlekova, Zuzana Sycova-Mila, Boris Kollarik, Ramadan Aziri, Daniel Pindak, Jozef Mardiak, Michal Chovanec, Peter Celec, Michal Mego
Summary: This study aims to analyze the association between plasma vitamin D and clinico-pathological findings and prognosis in patients with germ-cell tumors (GCTs). The results showed that low plasma vitamin D levels were associated with an unfavorable response to therapy and disease recurrence. However, it is still unknown whether low vitamin D has a causative role in the disease and whether its supplementation affects the outcome.
FRONTIERS IN ONCOLOGY
(2023)
Article
Oncology
Michal Chovanec, Katarina Kalavska, Jana Obertova, Patrik Palacka, Katarina Rejlekova, Zuzana Sycova-Mila, Zuzana Orszaghova, Peter Lesko, Valentina De Angelis, Lucia Vasilkova, Daniela Svetlovska, Beata Mladosievicova, Jozef Mardiak, Michal Pastorek, Barbora Vlkova, Peter Celec, Michal Mego
Summary: This study found that disruption of the intestinal barrier may contribute to cognitive impairment in survivors of testicular germ cell tumors through the gut-blood-brain axis. Higher levels of plasma sCD14 were associated with worse cognitive function in these patients. These findings provide new insights into understanding and addressing cognitive impairment in survivors of testicular germ cell tumors.
FRONTIERS IN ONCOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Michaela Mihal'ova, Nadja Supcikova, Alexandra Gaal Kovalcikova, Jan Breza Jr, L'ubomira Tothova, Peter Celec, Jan Breza Sr
Summary: Extracellular DNA (ecDNA) in the plasma and urine of urinary tract infection (UTI) patients was studied to understand its dynamics. The study found that urinary ecDNA levels decreased significantly during hospitalization and remained low during follow-up. Plasma ecDNA levels did not change, but plasma DNase activity increased during follow-up. C-reactive protein (CRP) and procalcitonin levels in plasma and urine were positively correlated with ecDNA, and urinary ecDNA levels were also correlated with urinary bacterial burden in a mouse UTI model.
Article
Biochemistry & Molecular Biology
Kristina Macakova, Petra Psenkova, Nadja Supcikova, Barbora Vlkova, Peter Celec, Jozef Zahumensky
Summary: Infection with SARS-CoV-2 during pregnancy and vaccination against COVID-19 do not appear to cause major disturbances in the balance between free radical production and antioxidant activity in the placenta, as indicated by biomarkers of oxidative damage and antioxidant capacity. There were no significant differences in extracellular DNA (ecDNA) levels or deoxyribonuclease activity in the umbilical cord plasma among the study groups. These findings suggest that the risk of oxidative stress in the placenta is not significantly affected by SARS-CoV-2 infection or COVID-19 vaccination during pregnancy.
Article
Pediatrics
Katarina Sebekova, Radana Gurecka, Melinda Csongova, Ivana Koborova, Peter Celec
Summary: Cardiometabolic risk factors in young age are linked to a higher risk of developing atherosclerotic cardiovascular disease later in life. Atherogenic dyslipidemia is closely associated with obesity and metabolic syndrome in young individuals. It is uncertain whether cardiometabolic risk factors are related to the atherogenic index of plasma (AIP = log (TAG/HDL-C) in lean individuals with low atherogenic risk. Both AIP and markers of cardiometabolic risk are continuous variables, leading us to hypothesize that their association would be linear before the onset of obesity and atherogenic dyslipidemia.