Article
Food Science & Technology
Daniel Plante, Julio Alexander Bran Barrera, Maude Lord, Irene Iugovaz, Neda Nasheri
Summary: A method for extracting viral RNA from raw oysters has been developed, showing high efficiency in removing RT-PCR inhibitors and reliably detecting viral contamination. The method outperformed the ISO 15216-1 method and showed comparable efficiency to Method E, with fewer steps and shorter hands-on time.
Article
Oncology
Amal A. Mohamed, Aymen S. Yassin, Basma S. Gomaa, Hossam Darwish, Rasha S. Mohamed, Sahar Makled, Ahmed Ramdan, Sherief Abd-Elsalam, Marwa M. Raafat
Summary: The study suggests that functional Survivin rs1042489 polymorphism may be associated with the risk of hepatocellular carcinoma, while Survivin rs8073069 polymorphism does not show a significant association with an increased risk of hepatocellular carcinoma in the studied groups.
CURRENT CANCER DRUG TARGETS
(2021)
Article
Plant Sciences
Amol D. Kokane, Sunil B. Kokane, Ashish J. Warghane, Mrugendra G. Gubyad, Ashwani Kumar Sharma, M. Krishna Reddy, Dilip Kumar Ghosh
Summary: ICRSV is a devastating pathogen affecting 'Kinnow mandarin' in northwest India, highlighting the need for sensitive and rapid detection methods.
Article
Chemistry, Multidisciplinary
Xander F. van Kooten, Yana Rozevsky, Yulia Marom, Efrat Ben Sadeh, Amit Meller
Summary: Most RNA-based COVID-19 diagnostic methods use enzymatic amplification for high sensitivity but rely on arbitrary thresholding. This study introduces a solid-state nanopore sensing technology for label-free quantification of SARS-CoV-2 RNA in clinical nasal swab samples. The method allows for ratiometric quantification of RNA abundance through single-molecule counting and length-based nanopore identification of dsDNA. The results show that this approach can provide quantitative positive outcomes in clinical samples classified as negative by RT-qPCR.
Article
Microbiology
Xue-Lian Luo, Xiu-Dan Zhang, Bei-Jie Li, Tian Qin, Zhi-Jie Cao, Qian-Jin Fan, Jing Yang, Dong Jin, Shan Lu, Ya-Yun Zheng, Xue-Fang Xu, Ji Pu, Jianguo Xu
Summary: Lassa virus is an important human pathogen causing hemorrhagic fever in West Africa, with an increasing risk of imported cases to other countries due to increased traveling. The high nucleotide diversity of the virus strains complicates the development of diagnostic assays. The GPC RT-PCR/2007 assay and the Mabsky kit showed good sensitivity and specificity for detecting most LASV strains.
MICROBIOLOGY SPECTRUM
(2023)
Article
Nanoscience & Nanotechnology
Zhengkang Li, Yuwei Di, Xiaoyan Song, Yanqi Wu, Yingye Feng, Xinqiang Zhang, Caiping Gong, Guanghua Li
Summary: In this study, a quantitative detection method for NoV GII was established, and the RT-LAMP detection system was found to be a viable alternative to RT-PCR for qualitative detection of NoV. Compared to RT-PCR, RT-LAMP offers shorter detection time and lower technical requirements.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2023)
Article
Microbiology
Rifat Tasnim Juthi, Saiful Arefeen Sazed, Md Fahad Zamil, Mohammad Shafiul Alam
Summary: This study evaluated and compared the performance of three commercially available COVID-19 RT-PCR kits, with the Aridia (R) COVID-19 Real-Time PCR Test showing the best performance among them. However, the Novel Coronavirus (2019-nCoV) Nucleic Acid Detection Kit performed the worst among the three kits.
Article
Entomology
Kirsten Warcup, Alex S. Torson, George D. Yocum
Summary: "Research on Sex Determination Methods of Colorado Potato Beetles" used publicly available RNA-seq data to select 5 candidate genes for sex-specific markers in adult Colorado potato beetles. The study confirmed that these 5 marker candidates exhibit a sex-specific expression pattern and can be used as PCR markers for sex determination. This method allows for simple sex detection using cDNA as the template and assigning sex to RNA-seq samples post hoc.
JOURNAL OF INSECT SCIENCE
(2023)
Article
Veterinary Sciences
Nahed Yehia, Fatma Eldemery, Abdel-Satar Arafa, Ahmed Abd El Wahed, Ahmed El Sanousi, Manfred Weidmann, Mohamed Shalaby
Summary: The H9N2 subtype of avian influenza A virus is circulating globally, causing severe economic losses. The developed H9N2 RT-RPA assay is highly sensitive and specific, allowing for rapid and accurate diagnosis of H9 aIAV infection.
VETERINARY SCIENCES
(2021)
Article
Microbiology
Hugo Alberto Barrera Saldana, Carolina Rivera Santiago, Raul Rodriguez Palacios
Summary: COVID-19 was first detected in Mexico in February 2020. Despite the initial lack of recognition by health authorities, we anticipated the need for viral detection tests and established a rapid molecular diagnostic service for SARS-CoV-2. Standardized protocols for biospecimen sampling, transportation, biobanking, preanalytical validation, and nucleic acids testing were implemented. The adoption of automation processes, a quality control/quality assurance system, and close collaboration with health authorities were key factors in our success.
MICROBIOLOGY SPECTRUM
(2022)
Article
Virology
Preeti Chhabra, Hannah Browne, Thalia Huynh, Marta Diez-Valcarce, Leslie Barclay, Margaret N. Kosek, Tahmeed Ahmed, Maria Renee Lopez, Chao-Yang Pan, Jan Vinje
Summary: This study validated a one-step conventional RT-PCR assay for dual typing of GI and GII noroviruses, successfully genotyping most known genotypes infecting humans. The use of broadly reactive genotyping assays greatly enhances the exchange of standardized genotype data globally to monitor trends in genotype diversity, which is essential for vaccine development and impact measurement.
JOURNAL OF CLINICAL VIROLOGY
(2021)
Article
Multidisciplinary Sciences
Xuexiang Lin, Xiao-Yu Liu, Bo Zhang, Ai-Qing Qin, Kwok-Min Hui, Kevin Shi, Yang Liu, Don Gabriel, X. James Li
Summary: A virus enzyme assay using a luciferin derivative as the substrate has been developed as an alternative method for detection of acute viral infection, particularly for influenza diagnosis. The assay showed similar detection rates for all influenza types and subtypes except for A(H7N9) due to lower viral titer. When tested with diluted specimens in comparison to a real-time polymerase chain reaction assay, the assay demonstrated high sensitivity and specificity.
SCIENTIFIC REPORTS
(2022)
Article
Multidisciplinary Sciences
Pawel Krawczyk, Anna Grenda, Paulina Terlecka, Justyna Blach, Kamila Wojas-Krawczyk, Tomasz Kucharczyk, Izabela Chmielewska, Robert Kieszko, Bozena Jarosz, Michal Gil, Katarzyna Reszka, Janusz Milanowski
Summary: The study evaluated the efficacy of genetic diagnostics in NSCLC patients and found that personalized treatment led to significantly prolonged survival compared to non-personalized treatments.
SCIENTIFIC REPORTS
(2021)
Article
Infectious Diseases
Bo Hu, Zhiyu Fan, Rulong Qiu, Mengmeng Chen, Houjun Wei, Yanhua Song, Weilong Liu, Weizhong Xu, Fang Wang
Summary: This study reports a novel recombinant strain of rabbit hemorrhagic disease virus 2 (RHDV2 or GI.2) detected from domestic rabbits in China. The new strain showed differences in infected time and mortality rate compared to the original strain, but similar viral load at the moribund stage of infection. The presence and circulation of this novel variant should be carefully monitored in China.
TRANSBOUNDARY AND EMERGING DISEASES
(2023)
Article
Multidisciplinary Sciences
Fredrikke Christie Knudtzen, Thoger Gorm Jensen, Susan Olaf Lindvig, Line Dahlerup Rasmussen, Lone Wulff Madsen, Silje Vermedal Hoegh, Malene Bek-Thomsen, Christian B. Laursen, Stig Lonberg Nielsen, Isik Somuncu Johansen
Summary: This study found that SARS-CoV-2 PCR C-q values in outpatients correlated with time after symptom onset but were not predictive of hospitalization. However, lower C-q values were associated with a higher risk of severe disease in hospitalized patients.
Article
Endocrinology & Metabolism
Marva Matboli, Ahmed E. M. Azazy, Seham Adel, Miram M. Bekhet, Sanaa Eissa
JOURNAL OF DIABETES AND ITS COMPLICATIONS
(2017)
Article
Oncology
Sanaa Eissa, Marwa Matboli, Nahla Awad, Yousif Kotb
Article
Multidisciplinary Sciences
Marwa Matboli, Sanaa Eissa, Doaa Ibrahim, Marwa G. A. Hegazy, Shalabia S. Imam, Eman K. Habib
SCIENTIFIC REPORTS
(2017)
Article
Oncology
Essam Eldin Mohamed Nour Eldin, Mahmoud Zaki El-Readi, Mohamed Mahmoud Nour Eldein, Albagir Ali Alfalki, Mohammad Ahmad Althubiti, Hala Fawzy Mohamed Kamel, Safaa Yehia Eid, Hiba Saeed Al-Amodi, Ahmad A. Mirza
CLINICAL BREAST CANCER
(2019)
Article
Medicine, General & Internal
Mohammad A. Althubiti, Mohamed M. Nour Eldein
SAUDI MEDICAL JOURNAL
(2018)
Article
Oncology
Sanaa Eissa, Maheera Safwat, Marwa Matboli, Ashraf Zaghloul, Maha El-Sawalhi, Amira Shaheen
UROLOGIC ONCOLOGY-SEMINARS AND ORIGINAL INVESTIGATIONS
(2019)
Article
Oncology
Essam Eldin Mohamed Nour Eldin, Mohamed Mahmoud Nour Eldein, Mahmoud Zaki El-Readi, Ahmad A. Mirza, Sameer Hasan Fatani, Hiba Saeed Al-Amodi, Mohammad Ahmad Althubiti, Esmail M. Al-Ezif, Safaa Yehia Eid, Hala Fawzy Mohammed Kamel
ONCOLOGY RESEARCH AND TREATMENT
(2020)
Article
Genetics & Heredity
Marwa Matboli, Doaa Ibrahim, Amany H. Hasanin, Mohamed Kamel Hassan, Eman K. Habib, Miram M. Bekhet, Ahmed M. Afifi, Sanaa Eissa
Summary: The study demonstrated the significant nephroprotective effect of isorhamnetin in a Type 2 diabetes mellitus rat model, showing improvements in blood glucose and lipid profiles, as well as modulation of autophagy-related genes and miRNA regulation.
Article
Biochemistry & Molecular Biology
Marwa Matboli, Marwa Mostafa Kamel, Nada Essawy, Meram Mohamed Bekhit, Basant Abdulrahman, Ghada F. Mohamed, Sanaa Eissa
Summary: The study constructed a ceRNA network associated with insulin resistance, validated its expression in clinical samples and cell lines, and successfully knocked out a specific lncRNA using CRISPR/Cas9. The findings highlight the significance of this network as a therapeutic target for gene editing in T2DM.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Marwa Matboli, Shaimaa H. Gadallah, Wafaa M. Rashed, Amany Helmy Hasanin, Nada Essawy, Hala M. Ghanem, Sanaa Eissa
Summary: This study aimed to construct a bioinformatics-based co-regulatory network of mRNAs and ncRNAs in NAFLD, which was validated in an animal model. The network revealed key players involved in NAFLD pathogenesis, with significant differences in expression levels between NAFLD and normal controls. This provides valuable insights for potential diagnostic and management strategies for NAFLD.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Medicine, General & Internal
Wesam A. Nasif, Abeer S. E. Ali, Asim A. Khogeer, Mohammed H. Mukhtar, Mohamed M. NourEldein, Ahmed Y. Shebly, Shumukh H. Alqahtani, Yahya A. Alnashri, Ghaidaa E. Khouj, Ziyad Gadah, Mohammad A. Althubiti
Summary: This study analyzed the impact and distribution of blood groups in different ethnicities and the susceptibility to COVID-19 infection in Makkah, Saudi Arabia. The results showed that blood types A and O were associated with higher infection rates, while blood type AB was associated with lower infection rates. Blood types A and O play a critical role in COVID-19 prognosis and vaccination priority.
SAUDI MEDICAL JOURNAL
(2022)
Article
Medicine, Research & Experimental
Wesam Ahmed Nasif, Mohammed Hasan Mukhtar, Abeer Shaker El-Moursy Ali, Mohamed Mahmoud Nour Eldein, Riyad Adnan Almaimani, Sami Sadagah Ashgar
Summary: The objective of this study was to determine the prevalence of Helicobacter pylori infection in a Saudi Arabian population and investigate its association with body mass index (BMI) and serum 8-hydroxy deoxyguanine (8-OHdG) levels. The results showed that obese individuals had a higher prevalence of H. pylori infection, and the levels of 8-OHdG were positively correlated with BMI in H. pylori-positive patients.
JOURNAL OF INTERNATIONAL MEDICAL RESEARCH
(2022)
Article
Medicine, Research & Experimental
Noha M. Hany, Sanaa Eissa, Manal Basyouni, Amany H. Hasanin, Yasmin M. Aboul-Ela, Nagwa M. Abo Elmagd, Iman F. Montasser, Mahmoud A. Ali, Paul J. Skipp, Marwa Matboli
Summary: This study constructed a genetic-epigenetic network linked to hepatic stellate cell (HSC) signaling and evaluated the effects of Mutaflor (R) on this network. The results showed that Mutaflor (R) administration minimized the alterations caused by NAFLD/NASH and reduced HSC activation and profibrogenic protein expression. This effect may be achieved through upregulation of RPARP AS-1 LncRNA and downregulation of miR-650, FOXA2 mRNA, and TEAD2 mRNA expression.
JOURNAL OF TRANSLATIONAL MEDICINE
(2022)
Article
Multidisciplinary Sciences
Noha Salah, Sanaa Eissa, Amal Mansour, Nagwa M. Abo El Magd, Amany Helmy Hasanin, Manal M. El Mahdy, Mohamed Kamel Hassan, Marwa Matboli
Summary: Non-alcoholic steatohepatitis (NASH), the aggressive variant of non-alcoholic fatty liver disease, can be regulated through the Hippo pathway. Probiotics, such as kefir milk, have been shown to effectively manage NASH. In this study, the efficacy of kefir milk in NASH management was investigated through the modulation of hepatic mRNA-miRNA panel linked to NAFLD/NASH Hippo signaling and gut microbita regulated genes. The results showed that kefir administration prevented steatosis and inflammatory component of NASH, improved liver function and lipid panel, and down-regulated the expression of miR 6807-5p while up-regulating the expression of SOX 11 and AMOTL2, resulting in the reduction of inflammatory and fibrotic markers. Overall, kefir suppressed NASH progression and improved fibrosis and hepatic inflammation.
SCIENTIFIC REPORTS
(2023)
Article
Endocrinology & Metabolism
Abdullatif Babakr, Mohamed Mukhtar, Mohamed Althubiti, Hiba Al-Amodi, Riyad Almaimani, Mohamed Mahmoud Nour Eldin, Mohamed Elzubeir Abdalla, Wesam Nasif
Summary: The aim of this study was to evaluate the association between lipid status and the levels of oxidized low-density lipoproteins and 8-hydroxy-2'-deoxyguanosine. The results showed a positive correlation between oxidized low-density lipoproteins and 8-hydroxy-2'-deoxyguanosine levels with hypercholesterolemia, hypertriglyceridemia, and high levels of low-density lipoproteins. This suggests that disturbance in lipid profile may result in increased levels of oxidized low-density lipoproteins and oxidative stress.
DIABETES METABOLIC SYNDROME AND OBESITY
(2023)