Article
Chemistry, Multidisciplinary
Changjiang Fan, Xinyue Meng, Wei Yang, Peiyan Wang, Wenguang Chang, Peifeng Li, Jianxun Wang
Summary: A labeling chemistry-based method, APSC-8-oxoGua-seq, is developed for sequencing 8-oxoGua in the microRNA transcriptome. The method uses N-(3-azidopropyl)-spermine-5-carboxamide (APSC) for selective labeling of 8-oxoGua, allowing the conjugation of a cleavable linker via the click reaction for 8-oxoGua pull-down and sequencing. APSC-8-oxoGua-seq enables identification of 8-oxoGua at single-base resolution.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Chenyu Cui, Ting-Hsuan Chen
Summary: In this study, a novel strategy for detecting hOGG1 activity based on CRISPR/Cas12a trans-cleavage triggered by cleavage ligation of a dumbbell DNA probe (DBP) designed with an 8-oxoG modification was developed. The method showed high sensitivity and selectivity, and demonstrated good compatibility for detecting hOGG1 activity in cell lysates, suggesting its potential application in disease diagnosis and scientific research.
Review
Chemistry, Analytical
Ana-Maria Chiorcea-Paquim, Ana Maria Oliveira-Brett
Summary: Oxidative DNA damage is crucial in the pathogenesis of various diseases, with 8-oxoG and 8-oxodG being important biomarkers for predicting oxidative stress levels. Electrochemical sensors based on modified electrodes are gaining interest due to their high sensitivity, selectivity, low-cost, and easy miniaturization and automation for detecting and quantifying these biomarkers in complex samples.
Review
Biochemistry & Molecular Biology
Ana-Maria Chiorcea-Paquim
Summary: Reactive oxygen species (ROS) continuously produced in living cells can cause oxidative DNA damage. The quantification of 8-oxoG and 8-oxodG, which are regarded as significant biomarkers for oxidative DNA damage, is essential for assessing the risk, diagnosing, and evaluating the treatment of various diseases. High-performance liquid chromatography with electrochemical detection (HPLC-ECD) is widely used for the determination of 8-oxoG and 8-oxodG in biological samples due to its selectivity and sensitivity.
Article
Medicine, General & Internal
Nihayet Bayraktar, Islim Guler, Mehmet Bayraktar, Ismail Koyuncu
Summary: This study aimed to reflect the rate of oxidative damage in the body among dyspeptic patients with Helicobacter pylori-positive peptic ulcer using 8-hydroxy-2'-deoxyguanosine (8-OHdG) level in serum samples and its association with bacterial endotoxin levels. Significant differences were found in 8-OHdG levels between healthy individuals and patients, with a weak correlation between OHdG and bacterial endotoxin revealed. Serum 8-OHdG may serve as a biomarker for the presence of peptic ulcers, highlighting the role of Helicobacter pylori in peptic ulcer pathogenesis.
INTERNATIONAL JOURNAL OF CLINICAL PRACTICE
(2021)
Article
Biochemistry & Molecular Biology
Jelena Mirnic, Milanko Djuric, Tanja Veljovic, Ivana Gusic, Jasmina Katanic, Karolina Vukoje, Bojana Ramic, Ana Tadic, Snezana Brkic
Summary: Oxidative stress may serve as a link between diabetes mellitus and periodontitis. In this study, the salivary lipid peroxidation (LP) in periodontitis patients with and without type 2 diabetes was evaluated. The results showed that the improvement in clinical periodontal status following nonsurgical periodontal therapy is accompanied by a significant decrease in salivary LP in diabetes mellitus type 2 (DM2) patients, suggesting that periodontitis is the primary driver of the elevated salivary LP in this group.
Article
Dentistry, Oral Surgery & Medicine
Emrah Turkmen, Erdem Veli Uzun, Fuat Bozaba, Nur Balci, Hilal Toygar
Summary: This study aimed to compare the levels of saliva and serum irisin and IL-6 between patients with stage III/grade B periodontitis and individuals with healthy periodontium. The results showed significantly higher levels of salivary and serum irisin and IL-6 in the periodontitis group. This suggests that salivary irisin and IL-6 could be potential biomarkers for predicting susceptibility to periodontitis.
CLINICAL ORAL INVESTIGATIONS
(2023)
Article
Dentistry, Oral Surgery & Medicine
Crystal Marruganti, Jacopo Traversi, Carlo Gaeta, Edoardo Ferrari Cagidiaco, Stefano Parrini, Nicola Discepoli, Simone Grandini
Summary: This study evaluated the association between adherence to Mediterranean diet and physical activity level on the periodontal status of a University-based cohort. The results showed that individuals with low adherence to Mediterranean diet and low physical activity level had 10 times the odds of having severe periodontitis compared to individuals with normal lifestyle habits.
JOURNAL OF PERIODONTOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Masaomi Gohbara, Noriaki Iwahashi, Hidefumi Nakahashi, Shunsuke Kataoka, Hironori Takahashi, Jin Kirigaya, Yugo Minamimoto, Eiichi Akiyama, Kozo Okada, Yasushi Matsuzawa, Masaaki Konishi, Nobuhiko Maejima, Kiyoshi Hibi, Masami Kosuge, Toshiaki Ebina, Teruyasu Sugano, Toshiyuki Ishikawa, Kouichi Tamura, Kazuo Kimura
Summary: High admission urinary 8-OhdG levels independently predict cardiovascular mortality and major cardiovascular events in patients with ACS.
Article
Biochemistry & Molecular Biology
Cristina Tortolini, Valeria Gigli, Antonio Angeloni, Federico Tasca, Nguyen T. K. Thanh, Riccarda Antiochia
Summary: The development of a novel voltammetric immunosensor for the detection of salivary MMP-8 at the point-of-care is described. The sensor showed good performance and comparable results to the conventional ELISA method when tested in real saliva samples. This biosensor is single-use, cost-effective, and requires a small quantity of test medium and a short preparation time.
BIOELECTROCHEMISTRY
(2024)
Article
Dentistry, Oral Surgery & Medicine
Mustafa Yilmaz, Esra Demir, Mervi Gursoy, Erhan Firatli, Vuokko Loimaranta, Ulvi Kahraman Gursoy
Summary: This study monitored the changes in salivary levels of B-cell activating factor (BAFF), tumor necrosis factor-like weak inducer of apoptosis (TWEAK), soluble (s)CD163 levels, and arginase activity in periodontitis patients after nonsurgical periodontal treatment. The results showed that periodontitis patients had higher levels of BAFF, sCD163, and arginase activity compared to healthy controls, but these levels gradually decreased after treatment. These findings suggest that treatment leads to a change in cell signaling, limiting the activation of B cells and reducing inflammatory response.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Review
Biochemistry & Molecular Biology
Ionut Luchian, Ancuta Goriuc, Darius Sandu, Mihai Covasa
Summary: Severe periodontitis is a destructive inflammatory disease that affects 11% of the global population. Metalloproteinases play a crucial role in cellular development and are associated with periodontal diseases. MMP-8 and MMP-9 are valuable diagnostic tools for periodontitis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Pharmacology & Pharmacy
Edwin K. Jackson, Elizabeth V. Menshikova, Vladimir B. Ritov, Zaichuan Mi, Lori A. Birder
Summary: This study demonstrates that a family of 8-aminopurines have renal excretory effects and may be useful for treating conditions such as hypertension, heart failure, and chronic kidney disease. 8-aminoguanine (or its prodrug 8-aminoguanosine) can induce diuresis, natriuresis, glucosuria, and antikaliuresis. If only diuresis and natriuresis are desired, 8-aminohypoxanthine or 8-aminoinosine may be useful. Furthermore, this study identifies 8-aminoinosine as a new endogenous 8-aminopurine.
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2022)
Article
Food Science & Technology
Sanja Kostic, Aleksandra Vilotic, Andrea Pirkovic, Dragana Dekanski, Suncica Borozan, Mirjana Nacka-Aleksic, Svetlana Vrzic-Petronijevic, Milica Jovanovic Krivokuca
Summary: This study investigated the cytoprotective effects of caffeic acid on first trimester extravillous trophoblast cells. The results showed that caffeic acid had no cytotoxic effects and could attenuate oxidative damage, reduce cytotoxicity and lipid peroxidation, and restore antioxidant capacity in trophoblast cells.
FOOD AND CHEMICAL TOXICOLOGY
(2022)
Article
Environmental Sciences
Junjie Zhang, Eivind B. Sundfor, Rolf Klokkerengen, Susana Gonzalez, Vasco C. Mota, Carlo C. Lazado, Alexandros G. Asimakopoulos
Summary: An UPLC-ESI-MS/MS method was developed for simultaneous determination of oxidative stress biomarkers in Atlantic salmon parr, with accurate quantification using internal standards. The dorsal fin was introduced as a possible alternative matrix for oxidative stress assessment. 8OHDG and DIY were used as biomarkers for the first time in monitoring fish health in RAS.