Article
Infectious Diseases
P. Urbanowicz, R. Izdebski, A. Baraniak, D. Zabicka, W. Hryniewicz, M. Gniadkowski
Summary: This study identified the molecular and genomic background of the rapid increase of MPPA in Poland, with multi-clonal spread as the leading factor. Various novel and specific characteristics of MPPA were also revealed through the analysis.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Infectious Diseases
Zhongliang Zhu, Juan Xu, Fang He
Summary: This study presented the first genome sequence of a multidrug-resistant Achromobacter xylosoxidans ST273 strain (MTYH1) isolated from a wound infection in China, providing insights into its antimicrobial resistance mechanisms, genomic features, and phylogenetic characteristics.
JOURNAL OF GLOBAL ANTIMICROBIAL RESISTANCE
(2021)
Article
Biotechnology & Applied Microbiology
Sanjay Singh, Ashutosh Pathak, Nida Fatima, Chinmoy Sahu, Kashi Nath Prasad
Summary: OXA-48-like carbapenemases were detected in 40% of clinical isolates of Escherichia coli and Klebsiella pneumoniae. These isolates also carried other carbapenemases, extended spectrum beta-lactamases (ESBLs) and 16S rRNA methyltransferases. OXA-48 producers exhibited high clonal diversity and carried transferable plasmids. Strict surveillance and detection methods are crucial for preventing the dissemination of OXA-48-like carbapenemases.
Article
Multidisciplinary Sciences
Jale Boral, Zeliha Genc, Fatihan Pinarlik, Guz Ekinci, Mert A. Kuskucu, Pelin Irkoren, Mahir Kapmaz, Suda Tekin, Nahit Cakar, Evren Senturk, Fatma Yurdakul, Bilge Dikenelli, Fusun Can, Onder Ergonul
Summary: This study describes the increased rate of Acinetobacter baumannii infections during the COVID-19 pandemic and defines its significance within the last five years. The findings show that the rate of A. baumannii infection during the pandemic was 1.90 times higher compared to the pre-pandemic era. The introduction of enhanced and customized infection control measures has resulted in the containment of an A. baumannii outbreak.
SCIENTIFIC REPORTS
(2022)
Review
Microbiology
Zoubida Dendani Chadi, Marie-Anne Arcangioli
Summary: DNA fingerprinting using PFGE is a widely used method to separate large DNA molecules and distinguish between different strains. It involves isolating intact chromosomal DNA and cutting it into restriction fragments for comparison. PFGE analysis provides valuable information for epidemiological investigations of bacterial pathogens.
Article
Multidisciplinary Sciences
Hassan A. Hemeg
Summary: The study showed that chickens are the main natural reservoir host of Salmonella infection for humans, with a high degree of identity between recovered strains from food samples and outpatient clinics. Regulations for handling chicken products should be updated to reduce Salmonella infections.
JOURNAL OF KING SAUD UNIVERSITY SCIENCE
(2021)
Article
Microbiology
Chenyang Cao, Qinya Niu, Jia Chen, Xuebin Xu, Huanjing Sheng, Shenghui Cui, Bin Liu, Baowei Yang
Summary: The study analyzed 292 clinical isolates of S. Enteritidis in Shanghai between 2006 and 2014, showing a high frequency of cefotaxime-resistant isolates with CTX-M-55 being the most prevalent genotype. The findings highlight the importance of monitoring CTX-M-55 and using cephalosporins, especially cefotaxime, cautiously in empirical treatment for Salmonella infections.
Article
Microbiology
Tran Thi Thuy Duong, Ya-Min Tsai, Li-Li Wen, Hui-Chuan Chiu, Pek Kee Chen, Tran Thi Dieu Thuy, Pei-Yun Kuo, Jazon Harl Hidrosollo, Shining Wang, Yen-Zhen Zhang, Wei-Hung Lin, Ming-Cheng Wang, Cheng-Yen Kao
Summary: This study investigates the molecular characteristics of carbapenemase genes in carbapenem-resistant Enterobacterales (CRE) isolates in Taiwan. It reveals an increase in CPE isolates and their association with PFGE typing.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Fatema-Tuz Johura, Sahitya Ranjan Biswas, Shah M. Rashed, Mohammad Tarequl Islam, Saiful Islam, Marzia Sultana, Haruo Watanabe, Anwar Huq, Nicholas R. Thomson, Rita R. Colwell, Munirul Alam
Summary: In this study, the genomic DNA extracted from V. cholerae O1 strains isolated in Bangladesh during 2004-2014 was analyzed using pulsed-field gel electrophoresis. The majority of the strains belonged to clade A, while the rest were estuarine strains clustered in clades B and C, or in small clades D and E. Furthermore, analysis of El Tor strains from Asia, Africa, and the Americas showed that 85% of the total set of strains belonged to clade A, indicating their relatedness but lack of homogeneity. Overall, 15% of the global strains comprised multiple small clades or segregated as singletons, suggesting the presence of different ecotypes in different geographical regions.
INFECTION GENETICS AND EVOLUTION
(2022)
Article
Food Science & Technology
Xianying Zeng, Suling Lv, Cong Qu, Lan Lan, Dongmei Tan, Xiugui Li, Li Bai
Summary: The study investigated the serotype, antimicrobial resistance, and molecular typing of 843 Salmonella isolates recovered from diarrheic patients in Guangxi, China from 2014 to 2017. The results highlighted the diversity of Salmonella serotypes and the high prevalence of emerging multidrug-resistant (MDR) Salmonella in Guangxi, emphasizing the need for enhanced surveillance of these MDR isolates to protect public health.
Article
Infectious Diseases
M. Biedrzycka, P. Urbanowicz, A. Guzek, S. Brisse, M. Gniadkowski, R. Izdebski
Summary: The study assessed the spread of NDM-1-producing Klebsiella pneumoniae ST147 organisms in Poland since 2015, demonstrating a high degree of homogeneity and close relatedness to organisms spreading in the Mediterranean region. The isolates showed a strict outbreak cluster with minor variations in resistome and plasmid replicon profiles, indicating continuous spread in Poland.
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2021)
Article
Biotechnology & Applied Microbiology
E. Mazengia, J. Meschke, K. Greeson, S. Zhao, J. Abbott, K. Eckmann, H. Tate, X. Huang, M. Samadpour
Summary: The study found indistinguishable PFGE profiles across poultry processors and years, as well as between poultry products and clinical isolates. Two Salmonella serotypes were most frequently isolated from raw poultry, and these two serotypes were also most frequently matched to clinical profiles. This implicates poultry as the main source of human Salmonella infections.
JOURNAL OF FOOD PROTECTION
(2021)
Article
Engineering, Chemical
Jiaxin Huang, Yatian Zhang, Chunxian Tao, Shanni Li, Qingxiang You, Dawei Zhang, Zhenqing Li, Yoshinori Yamaguchi
Summary: The study systematically investigated the separation performance of proteins with different sizes under square wave and inversion electric field. Results showed that there was a resonance frequency for protein molecule re-orientation under the same forward and backward time duration. The apparent mobility was positively correlated to modulation depth if below 200%, while it was negatively correlated if above 200%. Through PFCE, albumin and alpha-globulins in human serum were resolved within 7 minutes, indicating potential for improved protein analysis by PFCE.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2022)
Article
Chemistry, Analytical
Maribel Santiago Rodriguez, Juan C. Bravata Alcantara, Juan C. Martinez Briseno, Eduardo Diaz Escamilla, Ileana A. Cortes Ortiz, Maximo B. Martinez Benitez, Lilia Lopez-Canovas
Summary: This study presents a rapid and cost-effective protocol for molecular subtyping of Klebsiella pneumoniae strains using contour clamped homogeneous electric field (CHEF) electrophoresis, which is suitable for laboratories where the technique is already implemented. The approach allows for reliable and reproducible subtyping of isolates, facilitating infection control efforts.
ANALYTICAL LETTERS
(2021)
Article
Biochemical Research Methods
Karen Leon Arcia, Heidi Quintero Alvarez, Laura Maria Pantoja-Echevarria, Yainelis Garrido Nicot
Summary: This paper presents a simple and rapid protocol for DNA preparation from up to 24 bacterial isolates simultaneously, which avoids repeated transference of biological material and yields high quality genomic DNA. This method is of great significance for molecular subtyping of pathogens during disease outbreaks.
JOURNAL OF MICROBIOLOGICAL METHODS
(2022)
Article
Infectious Diseases
David M. Livermore, Daniele Meunier, Katie L. Hopkins, Michel Doumith, Robert Hill, Rachel Pike, Peter Staves, Neil Woodford
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2018)
Article
Infectious Diseases
Deryla T. D. Kenna, Alice Fuller, Kate Martin, Claire Perry, Rachel Pike, Phillipa J. Burns, Omendra Narayan, Stuart Wilkinson, Robert Hill, Neil Woodford, Julie M. J. Logan, Jane F. Turton
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2018)
Article
Public, Environmental & Occupational Health
V Decraene, S. Ghebrehewet, E. Dardamissis, R. Huyton, K. Mortimer, D. Wilkinson, K. Shokrollahi, S. Singleton, B. Patel, J. Turton, P. Hoffman, R. Puleston
JOURNAL OF HOSPITAL INFECTION
(2018)
Letter
Infectious Diseases
Tomas-Paul Cusack, Vilayouth Phimolsarnnousith, Khuanta Duangmala, Phonelavanh Phoumin, Jane Turton, Katie L. Hopkins, Neil Woodford, Nandini Shetty, Nicole Stoesser, Hang T. T. Phan, David A. B. Dance
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2019)
Article
Microbiology
Dervla T. D. Kenna, Amy Coward, Claire Perry, Rachel Pike, Ulf Schaefer, Jack Turton, Heather Green, Andrew M. Jones, Rowland J. Bright-Thomas, Phillipa Burns, Omendra Narayan, Stuart Wilkinson, Jane F. Turton
JOURNAL OF MEDICAL MICROBIOLOGY
(2019)
Article
Microbiology
Jane Turton, Frances Davies, Jack Turton, Claire Perry, Zoe Payne, Rachel Pike
Article
Immunology
Matthew J. Ellington, Frances Davies, Elita Jauneikaite, Katie L. Hopkins, Jane F. Turton, George Adams, Jiri Pavlu, Andrew J. Innes, Christopher Eades, Eimear T. Brannigan, Jacqueline Findlay, Leila White, Frances Bolt, Tokozani Kadhani, Yimmy Chow, Bharat Patel, Siddharth Mookerjee, Jonathan A. Otter, Shiranee Sriskandan, Neil Woodford, Alison Holmes
CLINICAL INFECTIOUS DISEASES
(2020)
Letter
Infectious Diseases
Katie L. Hopkins, Daniele Meunier, Nazim Mustafa, Rachel Pike, Neil Woodford
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2019)
Article
Respiratory System
Amy Coward, Dervla T. D. Kenna, Neil Woodford, Jane F. Turton, Malcolm Armstrong, Cressida Auckland, Ian Bowler, Phillipa Burns, James Cargill, Mary Carroll, William Flight, Michelle Graver, Heather Green, Carolyne Horner, Andrew Jones, Andrew M. Jones, Graeme Jones, Sarah Mayell, Jeorge Orendi, Audrey Perry, Ali Robb, Natasha Tucker, David Waine, Trevor Winstanley, Nick Withers
JOURNAL OF CYSTIC FIBROSIS
(2020)
Article
Microbiology
Jane F. Turton, Frances Davies, Surabhi K. Taori, Jack A. Turton, Stephanie L. Smith, Noshin Sajedi, Mandy Wootton
JOURNAL OF MEDICAL MICROBIOLOGY
(2020)
Article
Veterinary Sciences
James Paul Duff, Manal AbuOun, Steve Bexton, Jon Rogers, Jane Turton, Neil Woodford, Richard Irvine, Muna Anjum, Christopher Teale
Article
Microbiology
Thierry Wirth, Marine Bergot, Jean-Philippe Rasigade, Bruno Pichon, Maxime Barbier, Patricia Martins-Simoes, Laurent Jacob, Rachel Pike, Pierre Tissieres, Jean-Charles Picaud, Angela Kearns, Philip Supply, Marine Butin, Frederic Laurent
NATURE MICROBIOLOGY
(2020)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)