Article
Microbiology
Laura Veschetti, Marzia Boaretti, Giulia Maria Saitta, Rebeca Passarelli Mantovani, Maria M. Lleo, Angela Sandri, Giovanni Malerba
Summary: Bacteria belonging to the genus Achromobacter have emerged as pathogens causing a wide range of human infections, particularly in cystic fibrosis patients. This review examines the current literature on the role of Achromobacter in cystic fibrosis, focusing on taxonomy, prevalence in CF lung infections, genomic characteristics, and adaptation strategies.
MICROBIOLOGICAL RESEARCH
(2022)
Article
Microbiology
Shuying Yuan, Yanwen Chen, Kaicheng Lin, Lin Zou, Xinrong Lu, Na He, Ruijie Liu, Shaoxing Zhang, Danfeng Shen, Zhenju Song, Chaoyang Tong, Yizhi Song, Wenhong Zhang, Li Chen, Guiqin Sun
Summary: This study successfully determined the antimicrobial susceptibility of Elizabethkingia spp. using single cell Raman-DIP method and identified minocycline and levofloxacin as the first-line drugs for this infection. The method has significant clinical implications, reducing the report time and aiding in the treatment of the infection.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Angela Sandri, Laura Veschetti, Giulia Maria Saitta, Rebeca Passarelli Mantovani, Maria Carelli, Gloria Burlacchini, Sara Preato, Claudio Sorio, Paola Melotti, Anna Lisa Montemari, Ersilia V. Fiscarelli, Cristina Patuzzo, Caterina Signoretto, Marzia Boaretti, Maria M. Lleo, Giovanni Malerba
Summary: The Achromobacter spp. strains isolated from patients with chronic and occasional lung infection exhibit different virulence and antibiotic susceptibility features, which could be linked to their persistence in cystic fibrosis lungs. This highlights the potential of identifying predictive biomarkers of bacterial persistence for clinical purposes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Xubin Zheng, Ziwei Bao, Lina Davies Forsman, Yi Hu, Weihua Ren, Yazhou Gao, Xuliang Li, Sven Hoffner, Judith Bruchfeld, Jan-Willem Alffenaar
Summary: This study aimed to identify clinically relevant PK/PD thresholds for tuberculosis treatment optimization. Using CART analysis and LASSO logistic regression, the study found that AUC/MIC values and drug exposure of TB drugs were associated with clinical outcomes and adverse events. Further research is needed to explore the impact of CART-derived thresholds for individualized dosing on treatment outcomes in randomized controlled trials.
CLINICAL INFECTIOUS DISEASES
(2021)
Review
Microbiology
Asdren Zajmi, Jeanette Teo, Chew Chieng Yeo
Summary: Elizabethkingia spp. is a pathogenic bacterium that causes various diseases, especially in neonates and immunocompromised patients. Difficulties in accurately identifying the species of this bacterium using traditional methods may lead to an underestimation of the number of cases. The bacterium has an unusual antibiotic resistance pattern, suggesting an intrinsic origin for its multidrug resistance. Preventing and understanding Elizabethkingia spp. infections and limiting its spread is a new challenge.
Review
Critical Care Medicine
Patrick Maisonneuve, Albert B. Lowenfels
Summary: Cystic fibrosis is a progressive genetic disorder that affects the respiratory and digestive systems, and patients with CF are at an increased risk of digestive tract cancer. Guidelines for screening have been developed, but it is unknown whether new drugs will alter the risk of cancer in CF patients.
Article
Infectious Diseases
Margaret Ming-Chih Ho, Ming-Hui Sun, Wei-Chi Wu, Chi-Chun Lai, Lung-Kun Yeh, Yih-Shiou Hwang, Ching-Hsi Hsiao, Kuan-Jen Chen
Summary: This study evaluated the antibiotic susceptibility and minimum inhibitory concentrations of Stenotrophomonas maltophilia isolates from ocular infections. The results showed higher susceptibility and lower MICs for fluoroquinolones, which can be recommended for treating S. maltophilia ocular infections. Tigecycline and TMP-SMX could be alternative antibiotics.
Review
Nutrition & Dietetics
Carla Colombo, Gianfranco Alicandro, Valeria Dacco, Alessandra Consales, Fabio Mosca, Carlo Agostoni, Maria Lorella Gianni
Summary: Breastfeeding rates in CF patients are lower than those in the healthy population, and the benefits of breastfeeding for more than 2 months in this at-risk population are unclear. Limited studies suggest a potential benefit of breastfeeding in reducing lung infections, but data are inconsistent. Current available data are insufficient to draw definite conclusions on the benefits of exclusive breastfeeding in anthropometric and pulmonary outcomes in CF, and clinical trials evaluating well-defined breastfeeding promotion interventions are needed.
Article
Public, Environmental & Occupational Health
Kara Dean, Natalie Coaster, Kyana Young, Jade Mitchell
Summary: Elizabethkingia spp. is a common environmental pathogen that can cause infections, possibly transmitted through water. This study developed a dose-response model for Elizabethkingia spp. using frog and mice datasets. The estimated LD50 dose was approximately 237,000 CFU, and the median risk of infection for hospital patients was 3.4E-05. This is the first dose-response model developed for Elizabethkingia spp., which can aid future risk assessments and risk management strategies.
Article
Biology
Cristina Rodriguez-Melcon, Carlos Alonso-Calleja, Camino Garcia-Fernandez, Javier Carballo, Rosa Capita
Summary: This study determined the lethal concentrations of disinfectants and antibiotics for Listeria monocytogenes and found that the surface characteristics of bacterial cells influence resistance to these compounds. Most strains showed resistance to antibiotics, highlighting the concern of antibiotic resistance.
Article
Microbiology
Rundong Tan, Anqi Yu, Ziming Liu, Ziqi Liu, Rongfeng Jiang, Xiaoli Wang, Jialin Liu, Junhui Gao, Xinjun Wang
Summary: MIC is crucial for bacterial detection in infections, and predicting MIC accurately using genome sequencing and machine learning algorithms can improve clinical efficacy and antibiotic use.
FRONTIERS IN MICROBIOLOGY
(2021)
Letter
Immunology
J. Scott Weese, Kurtis E. Sobkowich, Zvonimir Poljak, Theresa M. Bernardo
Summary: We retrospectively reviewed culture and susceptibility results from 86 veterinary diagnostic laboratory results in US dogs and cats, finding 26 E. menigoseptica, 1 E. miricola, and 59 unspeciated Elizabethkingia isolates from 9 US states (2-22 isolates per state). Elizabethkingia infections in animals might pose an increased risk to humans.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Medicine, General & Internal
Axelle Schmoll, Claire Launois, Jeanne-Marie Perotin, Bruno Ravoninjatovo, Muriel Griffon, Sophie Carre, Pauline Mulette, Julien Ancel, Jean Hagenburg, Francois Lebargy, Gaetan Deslee, Jean-Hugues Salmon, Sandra Dury
Summary: This study found a high prevalence of rheumatologic pain in CF adult patients, which may have an impact on daily living, anxiety, and quality of life.
FRONTIERS IN MEDICINE
(2022)
Article
Public, Environmental & Occupational Health
Christine Rousset-Jablonski, Faustine Dalon, Quitterie Reynaud, Lydie Lemonnier, Clemence Dehillotte, Flore Jacoud, Marjorie Berard, Marie Viprey, Eric Van Ganse, Isabelle Durieu, Manon Belhassen
Summary: The incidence of cancer is higher in cystic fibrosis (CF) patients, especially those who have undergone lung transplantation. Specific follow-up, screening, and cancer prevention are necessary for CF patients with transplants.
FRONTIERS IN PUBLIC HEALTH
(2022)
Article
Microbiology
Jielu Yan, Bob Zhang, Mingliang Zhou, Francois-Xavier Campbell-Valois, Shirley W. I. Siu
Summary: Antimicrobial peptides (AMPs) are potential candidates for replacing conventional antibiotics to combat drug resistance in pathogenic bacteria. To accelerate the evaluation process, we develop a deep learning method called MBC-Attention to predict the minimum inhibitory concentration of AMPs against Escherichia coli. The proposed model outperforms traditional machine learning methods.
Article
Infectious Diseases
David M. Livermore, Daniele Meunier, Katie L. Hopkins, Michel Doumith, Robert Hill, Rachel Pike, Peter Staves, Neil Woodford
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2018)
Article
Infectious Diseases
Hannah Evans, Hikaru Bolt, Ellen Heinsbroek, Bryony Lloyd, Peter English, Samia Latif, Nicola Elviss, Jane Turton, Peter Hoffman, Paul Crook Supsup, Richard Puleston
Article
Public, Environmental & Occupational Health
V Decraene, S. Ghebrehewet, E. Dardamissis, R. Huyton, K. Mortimer, D. Wilkinson, K. Shokrollahi, S. Singleton, B. Patel, J. Turton, P. Hoffman, R. Puleston
JOURNAL OF HOSPITAL INFECTION
(2018)
Letter
Infectious Diseases
Tomas-Paul Cusack, Vilayouth Phimolsarnnousith, Khuanta Duangmala, Phonelavanh Phoumin, Jane Turton, Katie L. Hopkins, Neil Woodford, Nandini Shetty, Nicole Stoesser, Hang T. T. Phan, David A. B. Dance
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2019)
Article
Microbiology
Dervla T. D. Kenna, Amy Coward, Claire Perry, Rachel Pike, Ulf Schaefer, Jack Turton, Heather Green, Andrew M. Jones, Rowland J. Bright-Thomas, Phillipa Burns, Omendra Narayan, Stuart Wilkinson, Jane F. Turton
JOURNAL OF MEDICAL MICROBIOLOGY
(2019)
Article
Microbiology
Jane Turton, Frances Davies, Jack Turton, Claire Perry, Zoe Payne, Rachel Pike
Article
Immunology
Matthew J. Ellington, Frances Davies, Elita Jauneikaite, Katie L. Hopkins, Jane F. Turton, George Adams, Jiri Pavlu, Andrew J. Innes, Christopher Eades, Eimear T. Brannigan, Jacqueline Findlay, Leila White, Frances Bolt, Tokozani Kadhani, Yimmy Chow, Bharat Patel, Siddharth Mookerjee, Jonathan A. Otter, Shiranee Sriskandan, Neil Woodford, Alison Holmes
CLINICAL INFECTIOUS DISEASES
(2020)
Letter
Infectious Diseases
Katie L. Hopkins, Daniele Meunier, Nazim Mustafa, Rachel Pike, Neil Woodford
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2019)
Article
Respiratory System
Amy Coward, Dervla T. D. Kenna, Neil Woodford, Jane F. Turton, Malcolm Armstrong, Cressida Auckland, Ian Bowler, Phillipa Burns, James Cargill, Mary Carroll, William Flight, Michelle Graver, Heather Green, Carolyne Horner, Andrew Jones, Andrew M. Jones, Graeme Jones, Sarah Mayell, Jeorge Orendi, Audrey Perry, Ali Robb, Natasha Tucker, David Waine, Trevor Winstanley, Nick Withers
JOURNAL OF CYSTIC FIBROSIS
(2020)
Article
Microbiology
Jane F. Turton, Frances Davies, Surabhi K. Taori, Jack A. Turton, Stephanie L. Smith, Noshin Sajedi, Mandy Wootton
JOURNAL OF MEDICAL MICROBIOLOGY
(2020)
Article
Veterinary Sciences
James Paul Duff, Manal AbuOun, Steve Bexton, Jon Rogers, Jane Turton, Neil Woodford, Richard Irvine, Muna Anjum, Christopher Teale
Article
Microbiology
Thierry Wirth, Marine Bergot, Jean-Philippe Rasigade, Bruno Pichon, Maxime Barbier, Patricia Martins-Simoes, Laurent Jacob, Rachel Pike, Pierre Tissieres, Jean-Charles Picaud, Angela Kearns, Philip Supply, Marine Butin, Frederic Laurent
NATURE MICROBIOLOGY
(2020)
Article
Infectious Diseases
Syed Iqbal Haider, Naeem Akhtar, Muhammad Saleem, Sheraz Ahmed, Shiza Nadeem, Maham Amjad, Faiz Ul Haq
Summary: This study aimed to compare the diagnostic potential of Raman spectroscopy with rapid urease test and histopathology in diagnosing H. pylori infection. The results showed that Raman spectroscopy had higher sensitivity, accuracy, and specificity compared to the rapid urease test and histopathology. This study demonstrates the applicability of Raman spectroscopy as an innovative detection tool for molecular diagnosis of H. pylori infection in gastritis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Editorial Material
Infectious Diseases
Arati Mane, Reshu Agarwal, Meenu Bajpai, Suvarna Sane, Pallavi Vidhate, Partha Rakshit, Preeti Madan, Hema Gogia, Priya Abraham, Sandhya Kabra, Ekta Gupta
Summary: The present study aimed to determine the diagnostic performance of dried blood spot (DBS) for HBsAg and anti-HCV detection using CLIA in three different laboratories across India. DBS can be a simple and convenient alternative to plasma or serum for HBsAg detection, but site-specific validation of the assay is necessary for anti-HCV detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Somayeh Yazdanpanah, Maryam Rahbarmah, Marjan Motamedi, Hossein Khodadadi
Summary: This study compared the clinical characteristics of Fungitell and Dynamiker Fungus assays for diagnosing candidemia. The results showed that the DFA assay performed excellently, with high consistency with the FA assay and superior diagnostic performance.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Or Kriger, Natasha Belausov, Shiraz Gefen-Halevi, Nadezda Savieva, Sharon Amit
Summary: This study demonstrates that the usage of preservative-containing urine tubes may inhibit pathogens in low-volume pediatric urine samples, resulting in false-negative results. We advocate for large-scale validations by regulators to ensure result consistency.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Merve Zerey Albayrak, Sureyya Gul Yurtsever, Bilal Olcay Peker, Tuba Muderris, Selcuk Kaya
Summary: This study evaluated the antibody and T cell responses of homologous and heterologous booster doses for SARS-CoV-2 vaccines. The results showed that individuals who received heterologous boosters had higher antibody levels and higher levels of IFN-gamma, IL-2, and IL-13.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Sibra R. M. Shihab, Bushran N. Iqbal, Shiyamalee Arunasalam, Faseeha Noordeen
Summary: This study investigated the effect of sample-based RNA quality on COVID-19 real-time RT-PCR results. The purity of the extracts and concentration of RNA were found to impact test interpretations. The presence of impurities led to inconclusive test results.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ellen Vancutsem, Florence Crombe, Oriane Soetens, Magali Wautier, Corinna Dordelmann, Denis Pierard, Ingrid Wybo, Thomas Demuyser
Summary: Quantification of EBV DNA is crucial in transplantation settings for post-transplantation diagnosis. This study evaluated the performance of the AltoStar (R) EBV PCR Kit 1.5 on whole blood samples and found it to be reliable and accurate, with good sensitivity and linear range for EBV viral load determination.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Nouf K. Almaghlouth, Panagiotis Arvanitis, Kendra Vieira, Abby London, Dimitrios Farmakiotis
Summary: This study evaluated the performance of the Viracor CMV-T-cell immunity Panel (TCIP) in predicting CMV events. The results showed that CMV-specific CD4+ and CD8(+)T-cells were significantly lower in those with CMV events, and the TCIP could be a useful adjunct tool in individualized management of CMV infection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Linzhen Zhu, Haoting Zhang, Haibo Gu, Jianying Zhou
Summary: This case report describes a 70-year-old male patient with previously unknown immunodeficiency. Multiple pulmonary nodular shadows were observed on radiography. Fungal infection was detected in various samples, and the patient was ultimately diagnosed with disseminated Talaromyces marneffei infection and B-cell non-Hodgkin's lymphoma. The main significance of this study is to emphasize the importance for clinicians to obtain comprehensive specimens from patients presenting with multiple masses in order to ensure accurate clinical diagnosis.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Ming Chang, Sambasivan Venkatasubramanian, Holly Barrett, Kevin B. Urdahl, Kris M. Weigel, Gerard A. Cangelosi, Javeed A. Shah, Aparajita Saha, Libing Feng, Kristin N. Adams, David R. Sherman, Nahum Smith, Chetan Seshadri, Sean C. Murphy, James G. Kublin
Summary: Efforts are being made globally to develop vaccines and drugs against M. tuberculosis. This study developed a real-time RT-PCR assay to detect pre-rRNA as a biomarker for bacterial viability. The assay showed promising results in mouse models, indicating its potential in future clinical studies for tuberculosis drugs and vaccines.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Letter
Infectious Diseases
Temi Lampejo, Fadia Alsheikh, Declan Crilly, Martin Brown
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Yao Li, Pengfei Shi, Rong Zhu
Summary: This study successfully diagnosed a case of lung abscess caused by Porphyromonas endodontalis using mNGS technology, highlighting the importance of considering inhalation diseases caused by oral pathogens in patients with periodontal diseases and respiratory symptoms.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Article
Infectious Diseases
Mariana Quaresma de Souza, Dienefer Venske Bierhals, Ana Julia Reis, Erica Chimara, Julia Silveira Vianna, Andrea von Groll, Pedro Almeida da Silva, Ivy Bastos Ramis
Summary: This study proposed and evaluated a drug susceptibility testing method using 2,3,5-triphenyl tetrazolium chloride (TTC) as a colorimetric indicator for the clinical relevant non-tuberculous mycobacteria (Mycobacterium abscessus complex (MABC), M. avium complex (MAC), and M. kansasii). The results showed high agreement between TTC assay and the recommended broth microdilution method, indicating the potential of TTC as a promising indicator in drug susceptibility testing for NTM.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Shanshan Zhang, Mingyuan Duan, Shuang Li, Jie Hou, Ting Qin, Zhanwei Teng, Jianhe Hu, Huihui Zhang, Xiaojing Xia
Summary: Rapid detection of pathogenic microorganisms is crucial for epidemiologic identification, prevention, and control in public health. PCR-based methods have limitations in resource-limited areas due to the need for precise temperature control. Recombinase polymerase amplification (RPA) is a new nucleic acid amplification technology that can amplify DNA or RNA at a constant temperature, offering simplicity, high specificity and sensitivity, and short detection time. Microfluidic technology combined with RPA enables rapid detection of pathogenic microorganisms by integrating nucleic acid extraction, amplification, and detection.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)
Review
Infectious Diseases
Piyush Agrawal, Aditya Upadhyay, Awanish Kumar
Summary: Tuberculosis is a lethal infectious disease caused by Mycobacterium tuberculosis. During infection, the expression of host microRNAs changes, which could be potential biomarkers for early detection of tuberculosis. Further research is needed to identify potential microRNA biomarkers, but momentum is gaining in this field and microRNAs are expected to become routine approaches for reliable diagnosis and specific therapeutic interventions.
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE
(2024)