Article
Medical Laboratory Technology
Song Cai, Kimberly Gomez, Aubin Moutal, Rajesh Khanna
Summary: CaV3.2 channels play a crucial role in pain processing, with increased expression and activity in various pain models. Targeting proteins that regulate channel trafficking or transcription, as well as those that modify channels via post-translational modifications, may offer new opportunities for pain management.
TRANSLATIONAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Renfei Qi, Junping Cao, Yufang Sun, Yanping Li, Zitong Huang, Dongsheng Jiang, Xing-Hong Jiang, Terrance P. Snutch, Yuan Zhang, Jin Tao
Summary: In neuropathic pain, miR-32-5p plays a functional role by regulating Cav3.2 channels. Nerve injury-induced histone methylation decreases miR-32-5p expression, leading to increased Cav3.2 protein expression and pain-related behaviors.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Anesthesiology
Eder Gambeta, Maria A. Gandini, Ivana A. Souza, Gerald W. Zamponi
Summary: Ca(V)3.2 channels play an important role in trigeminal neuralgia, impacting the occurrence and characteristics of the disease. Mutations in these channels were found in patients, leading to functional changes and exacerbation of pain. By blocking these channels, the severity of pain can be reduced.
Article
Pharmacology & Pharmacy
Elodie Picard, Nicolas Kerckhove, Amaury Francois, Ludivine Boudieu, Elisabeth Billard, Frederic Antonio Carvalho, Gemma Bogard, Philippe Gosset, Justine Bourdier, Youssef Aissouni, Emmanuel Bourinet, Alain Eschalier, Laurence Daulhac, Christophe Mallet
Summary: T-type calcium channels, particularly the Ca(v)3.2 subtype, are crucial in inflammation and related pain. Inhibition of Ca(v)3.2 channels could be a promising strategy to relieve chronic inflammatory pain in patients, as shown in murine models. Targeting Ca(v)3.2 channels with pharmacological agents should be explored in clinical trials.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Cell Biology
Jie Lin, Weiming Guo, Qingtian Luo, Qingping Zhang, Teng Wan, Changyu Jiang, Yuanchun Ye, Haihuan Lin, Gang Fan
Summary: This study demonstrates that pharmaceutical intervention targeting cellular senescence can alleviate age-related dysfunction of the caveolar Ca(V)3.2-RyR axis and potentially ameliorate age-associated cardiovascular disease.
Review
Pharmacology & Pharmacy
Maricruz Rangel-Galvan, Violeta Rangel-Galvan, Alejandro Rangel-Huerta
Summary: Neuropathic pain is influenced by nerve damage or diseases that affect the somatosensory nervous system. Inflammatory and neuropathic pain are significant contributors when neurons are damaged or indirectly stimulated, with immune cells playing a crucial role. The study explores the modulation of hydrogen sulfide (H2S) on the Ca(v)3.2 T-type channels in neuropathic pain conditions, finding that H2S has a dual effect concentration-dependent effect on T-type channels, inhibiting at low concentrations and augmenting T-current at high concentrations. The CSE/H2S/Ca(v)3.2 pathway is identified as a potential therapeutic target for neuropathic pain disorders.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Cell Biology
Gui-zhou Li, Ya-hui Hu, Yi-ni Lu, Qing-yan Yang, Di Fu, Feng Chen, Yun-man Li
Summary: The study reveals that VCR therapy can cause neuropathic pain, and CaMKII and Ca(V)3.2 can activate astrocytes to mediate Cx43-dependent inflammation, further exacerbating the pain.
CELL BIOLOGY AND TOXICOLOGY
(2023)
Article
Neurosciences
Vinicius M. Gadotti, Sun Huang, Gerald W. Zamponi
Summary: The terpenes camphene and alpha-bisabolol derived from cannabis plants inhibit Cav3.2 T-type calcium channels, reducing nocifensive responses and thermal hyperalgesia in mice. These compounds also alleviate mechanical hypersensitivity in a mouse model of neuropathic pain, suggesting a wide spectrum of analgesic action by targeting Cav3.2 channels.
Article
Neurosciences
Tal Hoffmann, Katrin Kistner, Sonja L. J. Joksimovic, Slobodan M. Todorovic, Peter W. Reeh, Susanne K. Sauer
Summary: The study evaluated the role of the Ca(V)3.2 T-type calcium channel in painful diabetic neuropathy induced by hyperglycemia, showing significant changes in peripheral C-fiber functions.
EXPERIMENTAL NEUROLOGY
(2021)
Article
Biochemistry & Molecular Biology
Yu-Wang Chang, Yong-Cyuan Chen, Chien-Chang Chen
Summary: The Cav3.2 T-type calcium channel plays a crucial role in various pathological conditions, and its calcium channel function is regulated by phosphorylation by multiple kinases. In this study, we used quantitative mass spectrometry to identify the serine/threonine residues on Cav3.2 that are dephosphorylated by calcineurin. We found six dephosphorylated serine residues in different regions of Cav3.2, and observed that calcineurin binds to and dephosphorylates the C-terminus of Cav3.2. Additionally, we discovered that calcineurin can dephosphorylate a previously known CaMKII-phosphorylated site (S1198) and a novel CaMKII-phosphorylated site (S2137) on Cav3.2. These findings provide insights into the regulation of Cav3.2 through both kinase phosphorylation and calcineurin phosphatase dephosphorylation.
Article
Pharmacology & Pharmacy
Paz Duran, Santiago Loya-Lopez, Dongzhi Ran, Cheng Tang, Aida Calderon-Rivera, Kimberly Gomez, Harrison J. Stratton, Sun Huang, Ya-ming Xu, E. M. Kithsiri Wijeratne, Samantha Perez-Miller, Zhiming Shan, Song Cai, Anna T. Gabrielsen, Angie Dorame, Kyleigh A. Masterson, Omar Alsbiei, Cynthia L. Madura, Guoqin Luo, Aubin Moutal, John Streicher, Gerald W. Zamponi, A. A. Leslie Gunatilaka, Rajesh Khanna
Summary: This study identified argentatin C, a compound derived from the Native American medicinal plant Parthenium incanum, which can block the activity of voltage-gated sodium and calcium channels and has potential as a novel treatment for painful conditions. Experimental results demonstrated that argentatin C decreased ion currents and excitability in sensory neurons and relieved postsurgical pain in a mouse model. Therefore, argentatin C may serve as an alternative therapy for chronic pain management.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Physiology
Maria A. Gandini, Ivana A. Souza, Abhishek Kullar, Eder Gambeta, Gerald W. Zamponi
Summary: This study reveals the regulation of Ca(V)3.2 calcium channels by Rack-1 protein in a PKC-dependent manner, which affects calcium ion entry.
PFLUGERS ARCHIV-EUROPEAN JOURNAL OF PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Fumiko Sekiguchi, Nene Koike, Yasuhiro Shimada, Kaho Sugimoto, Hiroshi Masuda, Takashi Nakamura, Hiroaki Yamaguchi, Genzoh Tanabe, Shinsuke Marumoto, Yoshihito Kasanami, Maho Tsubota, Tsuyako Ohkubo, Shigeru Yoshida, Atsufumi Kawabata
Summary: Poly-trans-[(2-carboxyethyl)germasesquioxane] (Ge-132), an organogermanium, is hydrolyzed to 3-(trihydrox- ygermyl)propanoic acid (THGP) in aqueous solutions, and reduces inflammation, pain and cancer, whereas the underlying mechanisms remain unknown. It has been found that THGP can directly trap sulfides and inhibit sulfide-induced enhancement of Cav3.2 activity, leading to pain relief.
Article
Clinical Neurology
Donald Iain MacDonald, Ana P. Luiz, Federico Iseppon, Queensta Millet, Edward C. Emery, John N. Wood
Summary: Neuropathic pain leads to innocuous cooling being perceived as excruciating pain, yet the cell and molecular basis of this cold allodynia remains poorly understood. Research has found that in neuropathic pain states, normally silent large diameter cold-sensing neurons become sensitive to cooling, with many of them responding to noxious mechanical stimuli.
Article
Biochemistry & Molecular Biology
Marena Montera, Aleyah Goins, Leos Cmarko, Norbert Weiss, Karin N. Westlund, Sascha R. A. Alles
Summary: The study demonstrates the involvement of the Ca(v)3.3 T-type voltage-gated calcium channel subtype in chronic trigeminal neuropathic pain and suggests that blocking or attenuating its function may be an effective strategy for treatment. Furthermore, the results indicate that targeting Ca(v)3.3 could be more effective in female mice with trigeminal neuropathic pain in the FRICT-ION model.
Article
Biochemistry & Molecular Biology
Keon Ah Lee, Sang-Soo Lee, So Young Kim, Ah Reum Choi, Jung-Ha Lee, Kwang-Hwan Jung
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2015)
Article
Anesthesiology
Lorenza Dalla Massara, Hari Prasad Osuru, Azra Oklopcic, Desanka Milanovic, Srdjan Milan Joksimovic, Valentina Caputo, Michael Robert DiGruccio, Carlo Ori, Guangfu Wang, Slobodan Milenko Todorovic, Vesna Jevtovic-Todorovic
Article
Neurosciences
Aleksandar Lj. Obradovic, Navya Atluri, Lorenza Dalla Massara, Azra Oklopcic, Nikola S. Todorovic, Gaurav Katta, Hari P. Osuru, Vesna Jevtovic-Todorovic
MOLECULAR NEUROBIOLOGY
(2018)
Article
Neurosciences
Srdjan M. Joksimovic, Hari Prasad Osuru, Azra Oklopcic, Mark P. Beenhakker, Vesna Jevtovic-Todorovic, Slobodan M. Todorovic
MOLECULAR NEUROBIOLOGY
(2018)
Article
Biochemistry & Molecular Biology
Hari Prasad Osuru, Patcharin Pramoonjago, Mayuresh M. Abhyankar, Eric Swanson, LaToya Ann Roker, Helen Cathro, Prabhakara P. Reddi
MOLECULAR REPRODUCTION AND DEVELOPMENT
(2017)
Article
Multidisciplinary Sciences
Matthew Meiselman, Sang Soo Lee, Raymond-Tan Tran, Hongjiu Dai, Yike Ding, Crisalejandra Rivera-Perez, Thilini P. Wijesekera, Brigitte Dauwalder, Fernando Gabriel Noriega, Michael E. Adams
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Biochemical Research Methods
Minh Q. Tong, Md. Monirul Hasan, Sang Soo Lee, Md. Rezuanul Haque, Do-Hyoung Kim, Md. Shahidul Islam, Michael E. Adams, B. Hyle Park
BIOMEDICAL OPTICS EXPRESS
(2017)
Article
Anesthesiology
Robert H. Thiele, Keita Ikeda, Hari P. Osuru, Zhiyi Zuo
ANESTHESIA AND ANALGESIA
(2019)
Article
Biochemical Research Methods
Ryan Arvidson, Maayan Kaiser, Sang Soo Lee, Jean-Paul Urenda, Christopher Dai, Haroun Mohammed, Cebrina Nolan, Songqin Pan, Jason E. Stajich, Frederic Libersat, Michael E. Adams
MOLECULAR & CELLULAR PROTEOMICS
(2019)
Article
Neurosciences
Navya Atluri, Bianca Ferrarese, Hari P. Osuru, Ryan Sica, Caroline Keller, Zhiyi Zuo, Nadia Lunardi
Article
Neurosciences
Robert H. Thiele, Hari P. Osuru, Umadevi Paila, Keita Ikeda, Zhiyi Zuo
Article
Neurosciences
Sang Soo Lee, Mark N. Wu
CURRENT OPINION IN NEUROBIOLOGY
(2020)
Article
Neurosciences
Sang Soo Lee, Michael E. Adams
Summary: Endocrine state plays a crucial role in learning and memory in animals. In Drosophila, the rejection of male courtship by mated females leads to an aversive response known as courtship memory, which is enabled by the ecdysis triggering hormone (ETH) through its action in specific brain areas, indicating a convergence of ETH and juvenile hormone (JH) signaling in regulating persistent behavioral changes.
FRONTIERS IN NEUROSCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Sang Soo Lee, Yike Ding, Natalie Karapetians, Crisalejandra Rivera-Perez, Fernando Gabriel Noriega, Michael E. Adams