Article
Chemistry, Multidisciplinary
Yingjie Hang, Xiaoliang Ma, Chunxiao Liu, Siyuan Li, Sixuan Zhang, Ruyan Feng, Qianwen Shang, Qi Liu, Zhaozhao Ding, Xiaoyi Zhang, Liyin Yu, Qiang Lu, Changshun Shao, Hong Chen, Yufang Shi, Jiuyang He, David L. Kaplan
Summary: The development of anisotropic silk protein nanofiber-based hydrogels enables mouse embryonic stem cells to maintain stemness in vitro without the need for leukemia inhibitory factor and mouse embryonic fibroblasts, resulting in superior pluripotency and genetic stability. The hydrogels establish an improved dynamic niche that stimulates autocrine factor secretion and maintains pluripotency and propagation of ESCs, offering a simple and cost-effective option for expansion and utility of ESCs in research and therapy, while providing insights into early mammalian embryogenesis.
Article
Cell Biology
Anderson Tan, Renuka Prasad, Eek-hoon Jho
Summary: TFEB plays a crucial role in mESCs by regulating PTN to maintain pluripotency, with interactions involving Nanog and Sox2. While high levels of TFEB are expected to enhance autophagy-lysosomal activity, undifferentiated mESCs surprisingly display low basal levels.
CELL DEATH & DISEASE
(2021)
Article
Multidisciplinary Sciences
Andrea Lauria, Guohua Meng, Valentina Proserpio, Stefania Rapelli, Mara Maldotti, Isabelle Laurence Polignano, Francesca Anselmi, Danny Incarnato, Anna Krepelova, Daniela Donna, Chiara Levra Levron, Giacomo Donati, Ivan Molineris, Francesco Neri, Salvatore Oliviero
Summary: The establishment of DNA methylation patterns during mouse early development is crucial for cell fate determination. However, the specific molecular targets and mechanisms of de novo methylation machinery during differentiation are not fully understood. In this study, the researchers identified DNMT3B-dependent regulatory elements that play a critical role in cell fate determination. They found that DNMT3B-dependent DNA methylation is essential for proper differentiation and lineage determination, and ectopic expression of DNMT3B can restore normal gene expression and cell fate. This study provides important insights into the role of DNA methylation in early development and cell fate determination.
NATURE COMMUNICATIONS
(2023)
Article
Cell Biology
Oliver Brookes, Stephen D. Thorpe, Olga Rigby Evans, Michael C. Keeling, David A. Lee
Summary: This study investigated the relationship between biomechanical properties and pluripotency gene expression in murine embryonic stem cells, revealing considerable variation among cells, with high pluripotency gene expression correlating to lower cell stiffness.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kazuko Okamoto, Hideaki Fujita, Yasushi Okada, Soya Shinkai, Shuichi Onami, Kuniya Abe, Kenta Fujimoto, Kensuke Sasaki, Go Shioi, Tomonobu M. Watanabe
Summary: Nanog and Oct4 are core transcription factors that regulate pluripotency maintenance in mouse ESCs. The interaction time between Nanog and its target loci increases upon reduced expression or differentiation onset, suggesting a feedback mechanism for pluripotency maintenance. The expression level and interaction frequency of Nanog and Oct4 are correlated with their fluctuation and depend on ESC differentiation status. The DNA viscoelasticity near Oct4 target locus remains flexible during differentiation, supporting its role in chromatin opening or binding to uncondensed regions. These findings propose a new negative feedback mechanism for pluripotency maintenance via the DNA condensation state-dependent interplay of Nanog and Oct4.
Article
Cell Biology
Xinpeng Wen, Zesong Lin, Hao Wu, Lanrui Cao, Xudong Fu
Summary: In this study, the researchers identified a novel regulator, Zfp281, for the pluripotent-to-totipotent transition in mouse embryonic stem cells. They found that Zfp281 inhibits the transition by repressing transcripts upregulated during the 2C-like transition, and this inhibitory effect leads to impaired 2C-like transition ability in primed-state cells.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Zeba Sultana, Mathurin Dorel, Bertram Klinger, Anja Sieber, Ilona Dunkel, Nils Bluethgen, Edda G. Schulz
Summary: By combining systematic perturbation experiments with mathematical modeling, it was found that the number of active X chromosomes in mouse embryonic stem cells affects cell signaling and differentiation propensity. The presence of a single active X chromosome makes cells more sensitive to differentiation-promoting pathways and leads to a stronger negative feedback in the MAPK pathway. This may explain the delayed differentiation observed in female mouse embryonic stem cells.
MOLECULAR SYSTEMS BIOLOGY
(2023)
Article
Cell Biology
Jingsheng Li, Chunhong Dai, Wenyan Xie, Heyao Zhang, Xin Huang, Constantinos Chronis, Ying Ye, Wensheng Zhang
Summary: This study developed a one-step strategy to efficiently target and suppress endogenous pluripotent genes in mouse ESCs and replace their expression with AID-fused transgenes.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Masaki Kinoshita, Meng Amy Li, Michael Barber, William Mansfield, Sabine Dietmann, Austin Smith
Summary: Genome remethylation is crucial for mammalian development, with a study showing that deficiencies in Dnmt3a and Dnmt3b lead to ES cells developing into trophoblast cells due to failed suppression of Ascl2. Regulating the expression of Ascl2 can impact the transdifferentiation and contribution of ES cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Developmental Biology
Masaki Kinoshita, Toshihiro Kobayashi, Benjamin Planells, Doris Klisch, Daniel Spindlow, Hideki Masaki, Susanne Bornelov, Giuliano Giuseppe Stirparo, Hitomi Matsunari, Ayuko Uchikura, Ismael Lamas-Toranzo, Jennifer Nichols, Hiromitsu Nakauchi, Hiroshi Nagashima, Ramiro Alberio, Austin Smith
Summary: This study successfully established pluripotent stem cell lines from porcine, ovine, and bovine embryos without using feeder cells or serum factors. The cells showed differentiation in teratomas and in vitro, with global transcriptome analyses revealing commonality in transcription factor expression across the three species. The research also demonstrated the generation of cloned porcine fetuses using genetically modified AFX stem cells through nuclear transfer.
Article
Endocrinology & Metabolism
Constanza Contreras-Jurado, Ana Montero-Pedrazuela, Raul F. Perez, Susana Alemany, Mario F. Fraga, Ana Aranda
Summary: This study found that triiodothyronine (T3), a thyroid hormone, enhances the reprogramming of mouse embryonic fibroblasts (MEFs) into induced pluripotent stem cells (iPSCs). T3-induced iPSCs show similar characteristics to embryonic stem cells and have the potential for differentiation into the three major germ layers. The inclusion of T3 in reprogramming strategies could be useful for studying cell plasticity, disease, and regenerative medicine.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Yahong Wu, Weiwei Zhang
Summary: Pluripotent stem cells, including ESCs and iPSCs, are crucial for studying early development and treating diseases, with ubiquitination playing a key role in pluripotency regulation, particularly through the actions of E3 ligases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jana Pereckova, Michaela Pekarova, Nikoletta Szamecova, Zuzana Hoferova, Kristyna Kamarytova, Martin Falk, Tomas Perecko
Summary: NO2-OA modulates the pluripotency of mESC by reducing STAT3 phosphorylation, attenuating cardiac differentiation, and directing mESC into neural fate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Mikhail L. Vorobev, Bashar A. Alhasan, Irina I. Suvorova
Summary: Autophagy plays a critical role in early embryonic development, particularly in blastocyst formation. The absence of p53 does not affect autophagy activation in pluripotent cells but hinders differentiation induced by retinoic acid. Despite p53 deletion, Ulk1-dependent autophagy is still activated in response to serum deprivation in mESCs, but impaired during differentiation induced by retinoic acid.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Biology
Yaguang Zhang, Xiaowen Wan, Lei Qiu, Lian Zhou, Qing Huang, Mingtian Wei, Xueqin Liu, Sicheng Liu, Bo Zhang, Junhong Han
Summary: This study investigates the citrullination of Trim28 in embryonic stem cells (ESCs) and its role in pluripotency regulation. The results demonstrate that citrullination of Trim28 enhances its interaction with Smarcad1, preventing chromatin condensation. Additionally, citrullinated Trim28 promotes the transcription of pluripotency genes Nanog and Klf4, thus maintaining the pluripotency of ESCs.
SCIENCE CHINA-LIFE SCIENCES
(2023)
Article
Neurosciences
Hojun Lee, Kijeong Kim, Boa Kim, Junchul Shin, Sudarsan Rajan, Jingwei Wu, Xiongwen Chen, Michael D. Brown, Sukho Lee, Joon-Young Park
JOURNAL OF PHYSIOLOGY-LONDON
(2018)
Correction
Medicine, Research & Experimental
Dezhong Yang, Wenbin Fu, Liangpeng Li, Xuewei Xia, Qiao Liao, Rongchuan Yue, Hongmei Chen, Xiongwen Chen, Songzhu An, Chunyu Zeng, Wei Eric Wang
Article
Cardiac & Cardiovascular Systems
Ying Zhang, Wei Eric Wang, Xiaoying Zhang, Ying Li, Biyi Chen, Chong Liu, Xiaojie Ai, Xiaoxiao Zhang, Ying Tian, Chen Zhang, Mingxin Tang, Christopher Szeto, Xiang Hua, Mingxin Xie, Chunyu Zeng, Yingjie Wu, Lin Zhou, Weizhong Zhu, Daohai Yu, Steven R. Houser, Xiongwen Chen
CIRCULATION RESEARCH
(2019)
Editorial Material
Cardiac & Cardiovascular Systems
Xiongwen Chen, Xiaoying Zhang, Scott Gross, Steven R. Houser, Jonathan Soboloff
CIRCULATION RESEARCH
(2019)
Article
Nutrition & Dietetics
Jining Yang, Xiaoying Zhang, Long Yi, Ling Yang, Wei Eric Wang, Chunyu Zeng, Mantian Mi, Xiongwen Chen
NUTRITION & METABOLISM
(2019)
Article
Cardiac & Cardiovascular Systems
Polina Gross, Jaslyn Johnson, Carlos M. Romero, Deborah M. Eaton, Claire Poulet, Jose Sanchez-Alonso, Carla Lucarelli, Jean Ross, Andrew A. Gibb, Joanne F. Garbincius, Jonathan Lambert, Erdem Varol, Yijun Yang, Markus Wallner, Eric A. Feldsott, Hajime Kubo, Remus M. Berretta, Daohai Yu, Victor Rizzo, John Elrod, Abdelkarim Sabri, Julia Gorelik, Xiongwen Chen, Steven R. Houser
Summary: The joining region in JPH2 interacts with LTCC-alpha 1C subunit, causing LTCCs to distribute to dyads and colocalize with RyRs, facilitating dyad assembly and maintaining normal CICR in cardiomyocytes. Disruption of this interaction leads to T-tubule remodeling, impaired excitation-contraction coupling, and altered myocyte bioenergetics.
CIRCULATION RESEARCH
(2021)
Review
Physiology
Chong Liu, Ping Ke, Jingjing Zhang, Xiaoying Zhang, Xiongwen Chen
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Medicine, Research & Experimental
Lixiao Zhen, Qian Zhao, Jinhui Lu, Shengqiong Deng, Zhen Xu, Lin Zhang, Yuzhen Zhang, Huimin Fan, Xiongwen Chen, Zhongmin Liu, Yuying Gu, Zuoren Yu
MOLECULAR THERAPY-NUCLEIC ACIDS
(2020)
Article
Pharmacology & Pharmacy
Jing Wang, Tianshu Liu, Xiongwen Chen, Qiaofeng Jin, Yihan Chen, Li Zhang, Zhengyang Han, Dandan Chen, Yuman Li, Qing Lv, Mingxing Xie
Summary: Th17 cells play a significant role in the pathogenesis of myocarditis, and Bazedoxifene can affect STAT3 signaling to effectively inhibit the progression of cardiac inflammation. Therefore, Bazedoxifene may be a promising therapeutic strategy for the treatment of experimental autoimmune myocarditis.
FRONTIERS IN PHARMACOLOGY
(2021)
Editorial Material
Pharmacology & Pharmacy
Ya Liu, Xiongwen Chen, Hai-Gang Zhang
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Yijia Li, Xiongwen Chen, Yue Qi, Yichen Qu, Akshay Kumar, Songbo Dong, Yan Yang, Qiong Zhao
Summary: This study evaluated the impact of gender on Sievers types, valvulopathy, aortopathy, and outcomes of aortic valve replacement (AVR) in Chinese patients with bicuspid aortic valve (BAV). The results showed that male patients had more severe valvulopathy and aortopathy, as well as a higher risk of left ventricular dysfunction after AVR. Importantly, male patients developed these conditions at a younger age.
ECHOCARDIOGRAPHY-A JOURNAL OF CARDIOVASCULAR ULTRASOUND AND ALLIED TECHNIQUES
(2022)
Article
Cardiac & Cardiovascular Systems
Yijia Li, Jaslyn P. Johnson, Yijun Yang, Daohai Yu, Hajime Kubo, Remus M. Berretta, Tao Wang, Xiaoying Zhang, Michael Foster, Jun Yu, Douglas G. Tilley, Steven R. Houser, Xiongwen Chen
Summary: Maternal hypothyroidism can negatively impact the cardiac disease responses of offspring by reducing early postnatal cardiomyocyte proliferation and resulting in a smaller number of larger cardiac myocytes. These changes are associated with more severe cardiac disease responses following injury.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Xin Zhang, Yijia Li, Xiaoying Zhang, Valentino Piacentino, David M. Harris, Remus Berretta, Kenneth B. Margulies, Steven R. Houser, Xiongwen Chen
Article
Cell Biology
Tongtong Cao, Daniela Liccardo, Ryan LaCanna, Xiaoying Zhang, Rong Luz, Brian N. Finck, Tani Leigh, Xiongwen Chen, Konstantinos Drosatos, Ying Tian
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2019)
Meeting Abstract
Biotechnology & Applied Microbiology
Yinxia Xu, Junping Zhang, Xiaoying Zhang, Ping Guo, Helen Chew, Matthew Chrzanowski, Jenni Firrman, Nianli Sang, Xiongwen Chen, Yong Diao, Weidong Xiao
