Article
Multidisciplinary Sciences
Hui-Hao Lin, Meihua Christina Kuang, Imran Hossain, Yinan Xuan, Laura Beebe, Andrew K. Shepherd, Marco Rolandi, Jing W. Wang
Summary: This study explores the molecular and neuronal mechanisms underlying the transition from feeding to courtship in Drosophila melanogaster. The results show that feeding is prioritized over courtship in starved males, but the consumption of protein-rich food rapidly reverses this order. A gut-derived neuropeptide hormone called Diuretic hormone 31 (Dh31) plays a role in switching from feeding to courtship. The findings also reveal that Dh31 stimulates specific brain neurons through the circulation, leading to the prioritization of courtship over feeding through parallel pathways.
Article
Biochemistry & Molecular Biology
Meng-Hsuan Chiang, Yu-Chun Lin, Sheng-Fu Chen, Peng-Shiuan Lee, Tsai-Feng Fu, Tony Wu, Chia-Lin Wu
Summary: Thermosensation is critical for animal survival. This study reveals that nutritional status affects thermosensation in fruit flies, with hungry flies exhibiting stronger hot avoidance behavior. The activity of specific neurons in the brain is increased in hungry flies and these neurons receive the same level of hot input in both food-sated and hungry states. Insulin-like peptides regulate the activity of these neurons depending on feeding conditions. Surprisingly, different signaling pathways are involved in the regulation of hot avoidance behavior based on the type of insulin-like peptide. Finally, two specific neurons are found to be necessary for the output of integrated information related to hot avoidance behavior. These findings provide insights into the mechanisms of thermosensation modulation and its importance for animal survival.
Article
Cell Biology
Ferdi Ridvan Kiral, Suchetana B. Dutta, Gerit Arne Linneweber, Selina Hilgert, Caroline Poppa, Carsten Duch, Max von Kleist, Bassem A. Hassan, P. Robin Hiesinger
Summary: Genetic determinism is limited in affecting the variability of synapse numbers and partners, as shown by studying brain wiring and behavior in Drosophila with non-genetic perturbations like developmental temperature. Lower temperatures and slower development unexpectedly lead to increased axo-dendritic branching, synapse numbers, and non-canonical synaptic partnerships, while maintaining a robust ratio of canonical synapses. The findings suggest that genetically encoded relative and scalable synapse formation helps develop functional, but not identical, brains and behaviors.
Article
Biology
Dan Landayan, Brian P. Wang, Jennifer Zhou, Fred W. Wolf
Summary: Two central brain interneurons, Janu-GABA and Janu-AstA, regulate thirsty water seeking behavior in Drosophila. These neurons coordinate internal state drives to selectively control motivated seeking behavior.
Article
Biochemical Research Methods
Sven Dorkenwald, Claire E. McKellar, Thomas Macrina, Nico Kemnitz, Kisuk Lee, Ran Lu, Jingpeng Wu, Sergiy Popovych, Eric Mitchell, Barak Nehoran, Zhen Jia, J. Alexander Bae, Shang Mu, Dodam Ih, Manuel Castro, Oluwaseun Ogedengbe, Akhilesh Halageri, Kai Kuehner, Amy R. Sterling, Zoe Ashwood, Jonathan Zung, Derrick Brittain, Forrest Collman, Casey Schneider-Mizell, Chris Jordan, William Silversmith, Christa Baker, David Deutsch, Lucas Encarnacion-Rivera, Sandeep Kumar, Austin Burke, Doug Bland, Jay Gager, James Hebditch, Selden Koolman, Merlin Moore, Sarah Morejohn, Ben Silverman, Kyle Willie, Ryan Willie, Szi-chieh Yu, Mala Murthy, H. Sebastian Seung
Summary: FlyWire is an online community and platform for proofreading connectome data of the Drosophila brain, using interactive segmentation technology and collaborative editing to distribute proofreading work globally. By utilizing an open community, it accelerates proofreading by recruiting more participants and promotes scientific discovery through information sharing.
Article
Biology
Benedicte M. Lefevre, Stecy Mienanzambi, Michael Lang
Summary: The study found that the Sonoran desert endemic species Drosophila pachea is not sensitive to changes in rearing temperature, with similar duration of pupal development at different temperatures. This is likely an evolutionary mechanism to ensure proper development and adaptation to desert climate conditions.
JOURNAL OF THERMAL BIOLOGY
(2022)
Article
Biology
Soohong Min, Yangkyun Oh, Pushpa Verma, Samuel C. Whitehead, Nilay Yapici, David Van Vactor, Greg S. B. Suh, Stephen Liberles
Summary: The study reveals the essential role of Drosophila Piezo in volume-based control of meal size, with neurons expressing Piezo responding to food reservoir organ distension. Activating Piezo neurons decreases appetite, while knockout and silencing of Piezo lead to gut bloating and increased food consumption and body weight.
Review
Neurosciences
Ayden Gouveia, Rui de Oliveira Beleza, Sophie M. Steculorum
Summary: Recent studies have refined and re-evaluated our understanding of the regulatory principles and behavioral effects of AgRP circuits, revealing a fine-tuned regulation of their activity dynamics across the whole sequence of feeding-related behaviors.
EUROPEAN JOURNAL OF NEUROSCIENCE
(2021)
Article
Entomology
Hee Kyung Kim, Chae Jeong Kim, Daegyu Jang, Do-Hwan Lim
Summary: In this study, the upregulation of miR-274-5p was found to activate the JNK and JAK/STAT signaling pathways by suppressing the expression of found-in-neurons (fne), which controls the formation of melanotic masses and developmental growth in flies.
Review
Biochemistry & Molecular Biology
Dick R. Nassel, Shun-Fan Wu
Summary: Neuropeptides are diverse messenger molecules involved in regulating daily physiology and behaviors in metazoans. CCK/SK signaling plays important roles in satiety, feeding, gustatory sensitivity, locomotor activity, aggression, and reproductive behavior in both mammals and invertebrates, although the underlying mechanisms may differ.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2022)
Article
Genetics & Heredity
Neeraja Sanal, Lorena P. Keding, Ulrike Gigengack, Esther P. Michalke, Sebastian Rumpf
Summary: Neurite pruning and regrowth are important mechanisms for neural circuit adaptation. The PI3K/TORC1 pathway is required for dendrite regrowth after pruning in Drosophila peripheral neurons. TORC1 selectively stimulates the translation of regrowth factors involved in cytoskeletal regulation and secretion. The coordination of TORC1 in neurite pruning and regrowth is crucial for proper circuit development.
Review
Medicine, Research & Experimental
Kristie B. Yu, Elaine Y. Hsiao
Summary: The gut microbiota can influence host appetite by modulating food preference and interacting with neural pathways involving neurotransmitters and neuropeptides. Understanding the roles of gut microbes in feeding regulation is crucial for developing therapeutic strategies for metabolic and eating disorders.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Neurosciences
Gaia Tavosanis
Summary: Neuronal dendrites acquire complex morphologies through interactions with the cellular environment during development. Genetic approaches have identified factors controlling dendrite differentiation in vivo, suggesting localized molecular cascades are key to forming and stabilizing dendrite branches with neuron type-specific characteristics. Though challenging, studying neuronal dendrite differentiation in animals shows promising pathways for future research.
CURRENT OPINION IN NEUROBIOLOGY
(2021)
Article
Biology
Giovanni Frighetto, Mark A. Frye
Summary: Drosophila uses smooth optomotor movements to stabilize gaze and impulsive saccades to pursue vertical bars, with T4 and T5 cells providing inputs for gaze stabilization and T3 cells driving bar tracking body saccades. Silencing T3 reduces tracking saccades, while optogenetic manipulation affects saccade rate. Smooth optomotor responses remain unaffected. Parallel neural pathways coordinate gaze stabilization and saccadic bar tracking behavior in flight.
Article
Neurosciences
Austin B. Keeler, Amy L. Van Deusen, Irene Cheng, Corey M. Williams, Sarah M. Goggin, Ashley K. Hirt, Shayla A. Vradenburgh, Kristen Fread, Emily A. Puleo, Lucy Jin, Christopher D. Deppmann, Eli R. Zunder, Yipkin Calhan
Summary: This study presents a single-cell, protein-level atlas of mouse dorsal root ganglia and highlights the significance of protein-level measurements in identifying functional cell states during somatosensory development.
NATURE NEUROSCIENCE
(2022)
Article
Genetics & Heredity
Hongyu Ruan, Atsushi Ueda, Xiaomin Xing, Xuxuan Wan, Benjamin Strub, Spencer Mukai, Kaan Certel, David Green, Kyle Belozerov, Wei-Dong Yao, Wayne Johnson, Jim Jung-Ching Lin, Arthur J. Hilliker, Chun-Fang Wu
JOURNAL OF NEUROGENETICS
(2017)
Editorial Material
Biology
Wayne A. Johnson
Article
Neurosciences
Myung-Jun Kim, Wayne A. Johnson
Article
Developmental Biology
Joshua A. Ainsley, Myung Jun Kim, Lauren J. Wegman, Janette M. Pettus, Wayne A. Johnson
DEVELOPMENTAL BIOLOGY
(2008)
Article
Genetics & Heredity
Anke Vermehren-Schmaedick, Joshua A. Ainsley, Wayne A. Johnson, Shireen-A Davies, David B. Morton
Article
Genetics & Heredity
Myung Jun Kim, Joshua A. Ainsley, Justin W. Carder, Wayne A. Johnson
JOURNAL OF NEUROGENETICS
(2013)
Article
Multidisciplinary Sciences
Wayne A. Johnson, Justin W. Carder
Article
Biochemistry & Molecular Biology
JA Ainsley, JM Pettus, D Bosenko, CE Gerstein, N Zinkevich, MG Anderson, CM Adams, MJ Welsh, WA Johnson
Article
Neurosciences
L Liu, AS Leonard, DG Motto, MA Feller, MP Price, WA Johnson, MJ Welsh
Article
Neurosciences
L Liu, O Yermolaieva, WA Johnson, FM Abboud, MJ Welsh
NATURE NEUROSCIENCE
(2003)
Article
Multidisciplinary Sciences
L Liu, WA Johnson, MJ Welsh
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2003)
Article
Biochemistry & Molecular Biology
T Komiyama, WA Johnson, LQ Luo, GSXE Jefferis
Article
Developmental Biology
K Certel, A Hudson, SB Carroll, WA Johnson
Article
Neurosciences
Y Ma, K Certel, YP Gao, E Niemitz, J Mosher, A Mukherjee, M Mutsuddi, N Huseinovic, ST Crews, WA Johnson, JR Nambu
JOURNAL OF NEUROSCIENCE
(2000)
Review
Developmental Biology
Cheng Shi, Pengfei Jiao, Zhiyi Chen, Lan Ma, Siyue Yao
Summary: This review discusses the molecular etiology of congenital craniofacial abnormalities, with a focus on the role and mechanism of noncoding RNAs in regulating craniofacial development. Aberrant expression of noncoding RNAs has been implicated in the pathogenesis of craniofacial abnormalities, providing potential therapeutic targets.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Hideru Togashi, Steven Ray Davis, Makoto Sato
Summary: Tile patterns, regulated by cell adhesion molecules, are regular arrangements of cells that play important functional roles in multicellular organisms. The physical constraints and cell adhesion regulate both cell shape and tissue morphogenesis.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Armen Khanbabei, Lina Segura, Cynthia Petrossian, Aaron Lemus, Ithan Cano, Courtney Frazier, Armen Halajyan, Donnie Ca, Mariano Loza-Coll
Summary: This article investigates the genetic regulatory mechanisms of Drosophila intestinal stem cells. The study found that most target genes co-regulated by Esg and STAT show a consistent gene expression pattern. However, manipulating these validated targets in vivo rarely replicated the effects of manipulating Esg and STAT, suggesting the presence of complex genetic interactions among the downstream targets of these two master regulator genes.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Bayley J. Waters, Zoe R. Birman, Matthew R. Wagner, Julia Lemanski, Barak Blum
Summary: Researchers found that conditional deletion of Robo2 in adult mice led to a significant loss of islet architecture without affecting beta cell identity or function, suggesting that Robo2 plays a role in actively maintaining adult islet architecture. Understanding the factors required for islet architecture maintenance is crucial for developing future diabetes therapies.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Rhiannon Clements, Tyler Smith, Luke Cowart, Jennifer Zhumi, Alan Sherrod, Aidan Cahill, Ginger L. Hunter
Summary: Cell protrusions play a crucial role in regulating cell activities during development. By studying the regulation mechanism in fruit fly sensory bristle patterning, it was found that Myosin XV is essential for the dynamics of signaling filopodia and promotes long-range Notch signaling.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Margaret Keating, Ryan Hagle, Daniel Osorio-Mendez, Anjelica Rodriguez-Parks, Sarah I. Almutawa, Junsu Kang
Summary: Knock-in reporter (KI) animals are essential for studying gene expression in biomedical research. This study developed a new strategy using minicircle technology and a minimal promoter to enhance knock-in events and establish stable KI transgenic reporter lines. The study also highlighted the importance of selecting the proper KI line due to potential inappropriate influence of genome editing on reporter gene expression.
DEVELOPMENTAL BIOLOGY
(2024)
Article
Developmental Biology
Christian Altbuerger, Meta Rath, Daniel Armbruster, Wolfgang Driever
Summary: This study reveals that Neurog1 and Olig2 transcription factors have differential requirements for the development of dopaminergic neurons, and they integrate local patterning signals and Notch neurogenic selection signaling to specify the progenitor population and initiate neurogenesis and differentiation.
DEVELOPMENTAL BIOLOGY
(2024)