Article
Reproductive Biology
Samir Mehanovic, Raifish E. Mendoza-Villarroel, Karine de Mattos, Philippe Talbot, Robert S. Viger, Jacques J. Tremblay
Summary: COUP-TFII plays a crucial role in steroidogenesis, androgen homeostasis, cellular defense, and differentiation in mouse Leydig cells, with in-depth high-throughput transcriptomic analysis revealing novel gene pathways and networks regulated by this orphan nuclear receptor in depleted cells.
BIOLOGY OF REPRODUCTION
(2021)
Article
Andrology
Samir Mehanovic, Kenley Joule Pierre, Robert S. Viger, Jacques J. Tremblay
Summary: In this study, the researchers found that GATA4 and COUP-TFII cooperatively regulate gene expression, particularly the Amhr2 gene, in MA-10 Leydig cells through physical and functional interaction.
Article
Biochemistry & Molecular Biology
Li Li, Pierre Galichon, Xiaoyan Xiao, Ana C. Figueroa-Ramirez, Diana Tamayo, Jake J-K Lee, Marian Kalocsay, David Gonzalez-Sanchez, Maria S. Chancay, Kyle W. McCracken, Nathan N. Lee, Takaharu Ichimura, Yutaro Mori, M. Todd Valerius, Julia Wilflingseder, Dario R. Lemos, Elazer R. Edelman, Joseph Bonventre
Summary: COUP-TFII plays a crucial role in fibrosis, with its overexpression suppressing fatty acid oxidation, enhancing glycolysis pathways, and inducing fibroblasts to produce collagen. Genetic ablation of COUP-TFII can mitigate kidney fibrosis after injury. The regulation of PGC1α by COUP-TFII serves as an important mechanism in fibrosis.
Article
Multidisciplinary Sciences
Paola Aguiari, Yan-Yun Liu, Astgik Petrosyan, Sheue-yann Cheng, Gregory A. Brent, Laura Perin, Anna Milanesi
Summary: Thyroid hormone signaling, particularly through the interaction between COUP-TFII and THRA, plays a crucial role in muscle development and function, as well as in the maintenance of muscle mass. COUP-TFII is identified as a key factor in the abnormal muscle phenotype observed in THRA-PV mice, contributing to accelerated skeletal muscle loss with aging and impaired muscle regeneration after injury.
SCIENTIFIC REPORTS
(2021)
Article
Biochemistry & Molecular Biology
Giovan N. Cholico, Rance Nault, Tim R. Zacharewski
Summary: The study investigated the genomic binding and effects on gene expression of aryl hydrocarbon receptor (AhR), hepatocyte nuclear factor 4 (HNF4 alpha) and COUP transcription factor (COUP-TFII) in response to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD). The results suggested that TCDD caused the loss of liver-specific functions and markers of hepatocyte differentiation through interactions between AhR, COUP-TFII, and HNF4 alpha.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Mythily Ganapathi, Leticia Matsuoka, Michael M. March, Dong Li, Elly Brokamp, Sara Benito-Sanz, Susan White, Katherine Lachlan, Priyanka M. Ahimaz, Anshuman C. Sewda, Lisa Bastarache, Amanda Thomas-Wilson, Joan Stoler, Nuria Bramswig, Julia Baptista, Karen Stals, Florence Demurger, Benjamin Cogne, Bertrand Isidor, Maria Francesca P. Bedeschi, Angela D. Peron, Jeanne Amiel, Elaine Zackai, John D. Schacht, Alejandro D. Iglesias, Jenny Morton, Ariane Schmetz, Stephanie M. Undiagnosed Dis Network, Veronica Seidel, Stephanie D. Lucia, Stephanie T. Baskin, Isabelle K. Thiffault, Joy Cogan, Wendy Chung, Sarah Bowdin, Elizabeth Bhoj
Summary: NR2F2 gene is highly expressed during mammalian development and rare variants in NR2F2 are associated with congenital heart disease, congenital diaphragmatic hernia, and ovotesticular disorders/differences of sexual development. We reviewed 17 previously unreported individuals with rare NR2F2 variants and found a variable clinical spectrum including growth restriction, heart defects, diaphragmatic hernia, developmental delays, genital anomalies, and dysmorphic features. Our study expands the phenotype associated with NR2F2 variants.
EUROPEAN JOURNAL OF HUMAN GENETICS
(2023)
Article
Cell Biology
Stanislao Igor Travisano, Michael R. M. Harrison, Matthew E. Thornton, Brendan H. Grubbs, Thomas Quertermous, Ching-Ling Lien
Summary: Cardiac lymphatic vessels are vital for fluid balance, inflammation, disease, and heart regeneration. In this study, we investigate the development and molecular characteristics of cardiac lymphatics in human fetal hearts. We identify a population of lymphatic endothelial cells (LECs) in the epicardium, which are closely associated with coronary arteries. Additionally, we find that VEGFC is highly expressed in arterial endothelial cells and epicardium-derived cells, providing insights into the arterial association of cardiac lymphatic development. This study offers valuable knowledge and resources for understanding fetal heart development.
Article
Oncology
Heather M. Wojcik, Harold N. Lovvorn III, Melinda Hollingshead, Janene Pierce, Howard Stotler, Andrew J. Murphy, Suzanne Borgel, Hannah M. Phelps, Hernan Correa, Alan O. Perantoni
Summary: Wilms Tumor (WT) is the most common pediatric kidney cancer. Researchers have successfully cultured WT stem cells and established a cell model for studying WT, which is important for the research on drug efficacy and resistance.
FRONTIERS IN ONCOLOGY
(2023)
Article
Multidisciplinary Sciences
Diana Monsivais, Takashi Nagashima, Renata Prunskaite-Hyyrylainen, Kaori Nozawa, Keisuke Shimada, Suni Tang, Clark Hamor, Julio E. Agno, Fengju Chen, Ramya P. Masand, Steven L. Young, Chad J. Creighton, Francesco J. DeMayo, Masahito Ikawa, Se-Jin Lee, Martin M. Matzuk
Summary: The study reveals that bone morphogenetic proteins control endometrial receptivity in mice through the ACVR2A and SMAD1/5 signaling pathway, with ACVR2B being dispensable during embryo implantation.
NATURE COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Sang-Heum Han, Young Hee Kim, Su-Jeong Park, Jun-Gi Cho, Yoon Kyung Shin, Young Bin Hong, Jeanho Yun, Jin-Yeong Han, Hwan Tae Park, Joo-In Park
Summary: This study investigates the role of COUP-TFII in cAMP-induced Schwann cell differentiation. The results show that COUP-TFII regulates the expression of Oct6, Krox20, and myelin-related genes involved in Schwann cell differentiation and myelination. COUP-TFII may be involved in postnatal myelination by inducing Krox20 expression in Schwann cells.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Oncology
Simone Polvani, Sara Pepe, Sara Tempesti, Mirko Tarocchi, Giada Marroncini, Lapo Bencini, Elisabetta Ceni, Tommaso Mello, Lucia Picariello, Irene Simeone, Cecilia Grappone, Gabriele Dragoni, Lorenzo Antonuzzo, Elisa Giommoni, Stefano Milani, Andrea Galli
Summary: The expression of COUP-TFII_V2 in PDAC is associated with shorter overall survival and peripheral invasion, linked to epithelial to mesenchymal transition (EMT) and cancer progression. It may serve as a novel possible target for PDAC management.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2022)
Review
Cell Biology
Navin Gupta, Ryuji Morizane
Summary: The development of kidney organoid technology has revitalized the field of kidney developmental biology, allowing for the generation of nephron and collecting duct kidney organoids through directed differentiation protocols. However, there are limitations to current methods that need to be addressed for future improvements.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Medicine, Research & Experimental
Xiaoyan Wu, Xiulian Miao, Tinghui Shao, Shifan Tang, Yanshan Lin, Yong Xu, Nan Li, Tao Zhang
Summary: This study reveals that Slug is involved in regulating fibroblast-myofibroblast transition and renal fibrosis by modulating the expression of key transcription factors and ROS levels.
Article
Cell Biology
Manar Abboud Asleh, Mira Zaher, Jad Asleh, Julian Jadon, Lihi Shaulov, Ronit Yelin, Thomas M. Schultheiss
Summary: This study investigates the process of mesenchymal-epithelial transition (MET) in the chick embryo lateral mesoderm. Prior to MET initiation, mesenchymal cells exhibit non-polarized distribution of multiple polarity markers, albeit not aPKC. We present a stepwise model for MET, comprising polarization, 3D-rosette, and epithelialization stages.
DEVELOPMENTAL CELL
(2023)
Article
Cell Biology
Louise Cleal, Sophie L. McHaffie, Martin Lee, Nick Hastie, Ofelia M. Martinez-Estrada, You-Ying Chau
Summary: Congenital diaphragmatic hernia (CDH) is a common developmental defect with high morbidity. Research suggests that the formation of the diaphragm involves multiple cell types, with the etiology being complex and unclear. The study highlights the importance of non-muscle mesenchyme in diaphragm development, showing that abnormalities in this cell population can lead to CDH.
DISEASE MODELS & MECHANISMS
(2021)
Article
Dentistry, Oral Surgery & Medicine
H. -J. E. Kwon, S. Jia, Y. Lan, H. Liu, R. Jiang
JOURNAL OF DENTAL RESEARCH
(2017)
Article
Endocrinology & Metabolism
Han Liu, Jingyue Xu, Rulang Jiang
JOURNAL OF BONE AND MINERAL RESEARCH
(2019)
Article
Biology
Jingyue Xu, Han Liu, Yu Lan, Mike Adam, David E. Clouthier, Steven Potter, Rulang Jiang
Article
Dentistry, Oral Surgery & Medicine
Z. Liu, C. Li, J. Xu, Y. Lan, H. Liu, X. Li, P. Maire, X. Wang, R. Jiang
JOURNAL OF DENTAL RESEARCH
(2019)
Article
Dentistry, Oral Surgery & Medicine
Y. Lan, C. Qin, R. Jiang
JOURNAL OF DENTAL RESEARCH
(2019)
Article
Dentistry, Oral Surgery & Medicine
J. Xu, H. Liu, Y. Lan, J. S. Park, R. Jiang
JOURNAL OF DENTAL RESEARCH
(2020)
Article
Developmental Biology
Chaochang Li, Han Liu, Yueh-Chiang Hu, Yu Lan, Rulang Jiang
Article
Cell Biology
Hye Jung Ihn, Ju Ang Kim, Jiwon Lim, Sang-Hyeon Nam, So Hyeon Hwang, Young Kyung Kim, Jae-Young Kim, Jung-Eun Kim, Eui-Sic Cho, Rulang Jiang, Eui Kyun Park
Summary: Tooth root development involves the interaction of growth factors and transcription factors in Hertwig's epithelial root sheath and dental mesenchyme. Knockout of the Bbx gene in mice results in reduced length of tooth roots and downregulation of Dspp expression during early root formation, suggesting a role for Bbx in tooth root formation.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Anatomy & Morphology
Minghui Yue, Yu Lan, Han Liu, Zhaoming Wu, Toru Imamura, Rulang Jiang
Summary: The study demonstrates that Fgf18 expression in neural crest-derived craniofacial mesenchyme plays a critical role in the development of the mandible and multiple craniofacial bones, while Fgf18 expression in the palatal mesenchyme is dispensable for palatogenesis.
DEVELOPMENTAL DYNAMICS
(2021)
Article
Cell Biology
Jingyue Xu, Han Liu, Yu Lan, Rulang Jiang
Summary: The disruption of FOXF2 gene is associated with cleft palate in humans and mice. Mutations in the Foxf2 gene significantly affect the expression of neighboring genes, particularly Foxq1 and Exoc2. Through gene editing in mice, it was demonstrated that these mutations regulate palatal gene expression, emphasizing the importance of Foxf2 in development and disease.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Han Liu, Jingyue Xu, Yu Lan, Hee-Woong Lim, Rulang Jiang
Summary: This study investigated the molecular mechanisms of tendon development by generating a Scx(Flag) knockin mouse strain and identifying Scx direct target genes in developing tendon tissues. The results provide novel insights into the role of Scx in tendon development and homeostasis, offering valuable resources for further research on tendon cell differentiation and tissue regeneration. The Scx(Flag) mice present a useful tool for unraveling the molecular mechanisms underlying developmental and disease processes involving Scx.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Dentistry, Oral Surgery & Medicine
J-M Lee, C. Qin, O. H. Chai, Y. Lan, R. Jiang, H-J E. Kwon
Summary: Tooth agenesis is a common birth defect that occurs due to morphogenesis failure in early tooth development. This study shows that Msx1 regulates Wnt signaling by suppressing the expression of Dkk2 and Sfrp2, and that Sostdc1/Wise also plays a role in the odontogenic pathway downstream of Msx1. The findings suggest that modulation of Wnt signaling by Dkk2 and Sostdc1 is crucial for proper tooth morphogenesis.
JOURNAL OF DENTAL RESEARCH
(2022)
Article
Cell Biology
Paul P. R. Iyyanar, Zhaoming Wu, Yu Lan, Yueh-Chiang Hu, Rulang Jiang
Summary: The loss of ALX1 function leads to frontonasal dysplasia syndrome FND3, which is characterized by severe facial clefting and microphthalmia. This study generated an Alx1-deletion mouse model using CRISPR/Cas9-mediated genome editing and found that Alx1 is strongly expressed in frontonasal neural crest cells, playing a role in periocular and frontonasal mesenchyme development. Alx1(del/del) embryos showed increased apoptosis in periocular mesenchyme, decreased expression of ocular developmental regulators Pitx2 and Lmxb1, and disrupted frontonasal mesenchyme identity.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Developmental Biology
Paul P. R. Iyyanar, Chuanqi Qin, Nirpesh Adhikari, Han Liu, Yueh-Chiang Hu, Rulang Jiang, Yu Lan
Summary: The origin of the premaxilla bone in mammals has been a topic of debate. Recent studies suggest that it is derived from embryonic maxillary prominences instead of the frontonasal ectome-senchyme as previously thought. This study used genetic labeling techniques in mice to trace the contributions of different embryonic mesenchyme to the premaxillary bone, providing evidence for the developmental origin of the premaxilla.
DEVELOPMENTAL BIOLOGY
(2023)
Article
Cell Biology
Jingyue Xu, Paul P. R. Iyyanar, Yu Lan, Rulang Jiang
Summary: Mutations in SHH and other genes in the Hedgehog signaling pathway are associated with holoprosencephaly syndromes and craniofacial anomalies. SHH signaling plays crucial roles in craniofacial morphogenesis, including cell survival, growth and patterning, and organogenesis of various structures. This article summarizes recent advances in understanding the molecular and cellular mechanisms of SHH signaling and its involvement in the formation and patterning of craniofacial structures.