Article
Cell Biology
Xin Shi, Liyuan Jiang, Xin Zhao, Bei Chen, Wei Shi, Yanpeng Cao, Yaowu Chen, Xiying Li, Yusheng He, Chengjie Li, Xiaoren Liu, Xing Li, Hongbin Lu, Can Chen, Jun Liu
Summary: A functional scaffold combining CBD-bFGF and BDDM was developed to enhance stem cell interaction, improve endothelial inducibility, increase stem cell quantity, promote granulation tissue formation and angiogenesis, showing potential application for diabetic wound healing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Gensuke Okamura, Kosuke Ebina, Makoto Hirao, Ryota Chijimatsu, Yasukazu Yonetani, Yuki Etani, Akira Miyama, Kenji Takami, Atsushi Goshima, Hideki Yoshikawa, Takuya Ishimoto, Takayoshi Nakano, Masayuki Hamada, Takashi Kanamoto, Ken Nakata
Summary: Adding basic fibroblast growth factor (bFGF) to the culture of synovial mesenchymal stem cells (SMSC) can significantly promote cartilage regeneration in vivo, leading to better cartilage repair outcomes and more obvious cartilage matrix formation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Jeng-Chang Chen, Wendy Yang, Li-Yun Tseng, Hsueh-Ling Chang
Summary: This study explores the effects of neurosphere medium (NSM) and self-renewal medium (SRM) on the development of enteric neural stem cells (ENSCs) in vitro. The results show that the different compositions of NSM and SRM lead to distinct outcomes, with epidermal growth factor inhibiting enteric neuronal wiring in NSM, while basic fibroblast growth factor promotes neurogenesis and gangliogenesis in ENSCs.
Article
Pharmacology & Pharmacy
Leah R. Benington, Gunesh Rajan, Cornelia Locher, Lee Yong Lim
Summary: This study aimed to develop stable formulations of FGF-2 using pharmaceutical excipients to withstand physical stressors encountered in processing and storage. Formulations containing MC and alanine (F5) or MC and HSA (F6) were the most effective in stabilizing FGF-2 against thermal and processing stressors. These stabilized solutions have potential applications in medicinal products or in more advanced forms for wound healing and tissue regeneration.
Article
Endocrinology & Metabolism
Samantha Moron-Ros, Iiter Uriarte, Carmen Berasain, Matias A. Avila, Monica Sabater-Masdeu, Jose Maria Moreno-Navarrete, Jose Manuel Fernandez-Real, Marta Giralt, Francesc Villarroya, Aleix Gavalda-Navarro
Summary: The study demonstrates the crucial role of enterokine FGF15/19 in adipose tissue thermogenic adaptations. Elevated levels of FGF19 were found to be directly associated with UCP1 gene expression in subcutaneous adipose tissue. Experimental increase of FGF15 or FGF19 induced white fat browning in mice, while FGF15-null mice showed impaired white adipose tissue browning.
MOLECULAR METABOLISM
(2021)
Article
Biochemistry & Molecular Biology
Olga Grigorieva, Mikhail Arbatskiy, Ekaterina Novoseletskaya, Uliana Dyachkova, Alexander Ishkin, Natalia Kalinina, Anastasia Efimenko
Summary: The activation of multipotent mesenchymal stromal cells (MSCs) by PDGF-BB was found to induce the expression of genes associated with senescence, but further experimental validation demonstrated that PDGF-BB still exerted mitogenic and pro-migratory effects on MSCs, enhancing their pro-angiogenic activity without significantly stimulating MSC senescence.
Review
Immunology
Takeshi Nitta
Summary: The microenvironment of the thymus consists of thymic epithelial cells (TECs) and mesenchymal stromal cells, which play crucial roles in T cell development and selection. While TECs are well-studied, the immunological significance of mesenchymal stromal cells, particularly fibroblasts, has been less explored. Recent advancements in technology have allowed for the identification and characterization of these cells, revealing their unique functions in thymic organogenesis and T cell development.
INFLAMMATION AND REGENERATION
(2022)
Review
Endocrinology & Metabolism
Barbara Mensah Sankofi, Estefania Valencia-Rincon, Malika Sekhri, Adriana L. Ponton-Almodovar, Jamie J. Bernard, Elizabeth A. Wellberg
Summary: Obesity and type 2 diabetes are chronic metabolic diseases that have a significant impact on breast cancer risk and prognosis. Adipose tissue plays an important role in breast cancer progression, involving both adipocytes and fibroblasts. Fibroblast growth factor receptor signaling network may mediate the effects of adipose tissue on breast cancer.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Review
Cell Biology
Alessio Ardizzone, Valentina Bova, Giovanna Casili, Alberto Repici, Marika Lanza, Raffaella Giuffrida, Cristina Colarossi, Marzia Mare, Salvatore Cuzzocrea, Emanuela Esposito, Irene Paterniti
Summary: Cancer, the leading cause of death worldwide, requires successful strategies for treatment. Growth factors like VEGF, bFGF, and TGF-beta play significant roles in tumor growth, activating essential signaling pathways and promoting cell proliferation, angiogenesis, and metastasis. Specifically, bFGF is secreted by tumor cells and interacts with its receptors (FGFRs), leading to tumor cell proliferation, invasion, and upregulation of pro-inflammatory and anti-apoptotic proteins. Studies have focused on developing therapeutic strategies targeting the bFGF/FGFR axis, considering its involvement in oncogenesis. This review comprehensively examines the biological mechanisms of bFGF in the tumor microenvironment, available anticancer therapies targeting FGFRs, and the prognostic value of bFGF.
Article
Medicine, Research & Experimental
Qinrong Ping, Chunhui Wang, Xin Cheng, Yiming Zhong, Ruping Yan, Meng Yang, Yunqiang Shi, Xiangmeng Li, Xiao Li, Wenwen Huang, Liqiong Wang, Xiaofang Bi, Libing Hu, Yang Yang, Yingbao Wang, Rui Gong, Jun Tan, Rui Li, Hui Li, Jian Li, Wenju Wang, Ruhong Li
Summary: TGF-beta 1 plays a critical role in mediating crosstalk between bladder cancer cells and cancer-associated fibroblasts (CAFs), inducing epithelial-mesenchymal transition (EMT) through the FAP/VCAN axis. This finding is significant for understanding bladder cancer invasion and metastasis and provides potential strategies for diagnosis, treatment, and prognosis.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Cell & Tissue Engineering
Feifei Huang, Tianyun Gao, Wenqing Wang, Liudi Wang, Yuanyuan Xie, Chenxun Tai, Shuo Liu, Yi Cui, Bin Wang
Summary: The study showed that bFGF-overexpressing HUCMSCs met clinical safety standards and significantly improved therapeutic outcomes in a mouse SCI model. This included reducing glial scar formation, enhancing nerve regeneration and NSC proliferation, and improving locomotion function recovery. Additionally, bFGF-overexpressing HUCMSCs promoted NSC proliferation and neuronal differentiation in vitro through the PI3K-Akt-GSK-3 beta pathway, supporting the safety and efficacy of gene-modified MSCs for clinical use.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Biochemistry & Molecular Biology
Sangrok Park, Sejong Kim, Kyungmin Lim, Yeokyung Shin, Kwonwoo Song, Geun-Ho Kang, Dae Young Kim, Hang-Cheol Shin, Ssang-Goo Cho
Summary: In this study, we evaluated the utilization of thermostable basic fibroblast growth factor (TS-bFGF) for MSC culture and exosome production. The results showed that MSCs cultured with TS-bFGF had superior proliferation and produced a greater quantity of exosomes. These exosomes displayed anti-inflammatory and wound-healing effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Polymer Science
Mi Sun Kim, Yu-Shik Hwang, Hyo-Seol Lee, Ok Hyung Nam, Sung Chul Choi
Summary: The effect of controlled release growth factors on human pulp regeneration was investigated, revealing that FGF-2 and PDGF-BB at a concentration of 100 ng/mL significantly promote pulp cell proliferation.
Article
Cell & Tissue Engineering
Alessia Parascandolo, Michele Francesco Di Tolla, Domenico Liguoro, Manuela Lecce, Saverio Misso, Fabiana Micieli, Maria Rosaria Ambrosio, Serena Cabaro, Francesco Beguinot, Alessandra Pelagalli, Vittoria D'Esposito, Pietro Formisano
Summary: Platelet products are commonly used in regenerative medicine for wound healing, reduction of bleeding, tissue synthesis, and revascularization. Human platelet-rich plasma (hPRP) was found to support canine mesenchymal stem cells (cMSCs) by increasing their viability and migration. This study suggests that hPRP may be a promising tool for canine tissue regeneration and repair.
STEM CELLS INTERNATIONAL
(2023)
Article
Food Science & Technology
Wen-Juan Song, Pei-Pei Liu, Yan-Yan Zheng, Zi-Qing Meng, Hao-Zhe Zhu, Chang-Bo Tang, Hui-Xia Li, Shi-Jie Ding, Guang-Hong Zhou
Summary: This research demonstrates the construction of cultured fat using porcine adipose-derived mesenchymal stem cells on peanut wire-drawing protein scaffolds. The cultured fat showed similarities with porcine subcutaneous adipose tissue. This study holds great promise for improving the quality of cultured meat.
FOOD RESEARCH INTERNATIONAL
(2022)
Article
Cell & Tissue Engineering
Pierangela Palmerini, Anna Dalla Pieta, Roberta Sommaggio, Annavera Ventura, Giuseppe Astori, Katia Chieregato, Maria Chiara Tisi, Carlo Visco, Omar Perbellini, Marco Ruggeri, Elisa Cappuzzello, Antonio Rosato
Meeting Abstract
Oncology
A. Dalla Pieta, E. Cappuzzello, P. Palmerini, R. Sommaggio, G. Astori, K. Chieregato, O. Perbellini, M. Tisi, C. Visco, M. Ruggeri, A. Rosato
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2020)
Meeting Abstract
Oncology
A. Ventura, P. Palmerini, A. Dalla Pieta, R. Sommaggio, G. Astori, K. Chieregato, M. Tisi, C. Visco, O. Perbellini, M. Ruggeri, E. Cappuzzello, A. Rosato
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2020)
Review
Pharmacology & Pharmacy
Rachele Ciccocioppo, Patrizia Comoli, Giuseppe Astori, Francesca del Bufalo, Malvina Prapa, Massimo Dominici, Franco Locatelli
Summary: In the last 20 years, global regulatory frameworks have been addressing the challenges posed by using cellular products as new therapeutic tools. Advanced Therapy Medicinal Products have shown potential in changing the natural course of diseases like cancers, opportunistic infections, and chronic inflammatory conditions. Over 50 cell-based products have obtained market authorization globally, highlighting the increasing importance of developing cells as innovative therapeutic vehicles.
BRITISH JOURNAL OF PHARMACOLOGY
(2021)
Article
Medicine, Research & Experimental
Giuseppe Astori, Martina Bernardi, Angela Bozza, Daniela Catanzaro, Katia Chieregato, Anna Merlo, Monica Santimaria, Roberto Barbazza, Giuseppe Amodeo, Rachele Ciccocioppo, Francesca Elice, Marco Ruggeri
JOURNAL OF TRANSLATIONAL MEDICINE
(2020)
Article
Cell & Tissue Engineering
Rachele Ciccocioppo, Davide Gibellini, Giuseppe Astori, Martina Bernardi, Angela Bozza, Katia Chieregato, Francesca Elice, Stefano Ugel, Simone Caligola, Francesco De Sanctis, Stefania Cane, Alessandra Fiore, Rosalinda Trovato, Antonio Vella, Varvara Petrova, Giuseppe Amodeo, Monica Santimaria, Annarita Mazzariol, Luca Frulloni, Marco Ruggeri, Enrico Polati, Vincenzo Bronte
Summary: This study observed a decrease in inflammatory cells in both peripheral blood and bronchoalveolar lavage fluid, as well as a reduction in levels of pro-inflammatory cytokines like interleukin-6 and tumor necrosis factor-alpha after umbilical cord-derived MSCs treatment in COVID-19 patients. Additionally, an improvement in respiratory, renal, inflammatory, and pro-thrombotic indexes was also noted.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Oncology
Anna Dalla Pieta, Elisa Cappuzzello, Pierangela Palmerini, Annavera Ventura, Andrea Visentin, Giuseppe Astori, Katia Chieregato, Valentina Mozzo, Omar Perbellini, Maria Chiara Tisi, Livio Trentin, Carlo Visco, Marco Ruggeri, Roberta Sommaggio, Antonio Rosato
Summary: The study suggests an adoptive immunotherapy approach that combines CIK cells and CD20 antibody as an effective therapeutic strategy for aggressive B-cell malignancies, which can be rapidly translated into clinical practice without the need for genetic modifications.
JOURNAL FOR IMMUNOTHERAPY OF CANCER
(2021)
Article
Oncology
Daniela Catanzaro, Gloria Milani, Angela Bozza, Martina Bernardi, Katia Chieregato, Martina Menarin, Anna Merlo, Paola Celli, Romina Belli, Daniele Peroni, Alessandro Pozzato, Gianantonio Pozzato, Fabio Angelo Raneri, Lorenzo Volpin, Marco Ruggeri, Giuseppe Astori
Summary: This study investigates the anticancer activity of quantum molecular resonance (QMR) technology for glioblastoma treatment. The results show that QMR can significantly inhibit cancer cell proliferation and reduce tumor aggressiveness. QMR appears to work by affecting DNA replication, mitotic spindle assembly, and chromosome segregation. Moreover, QMR enhances the efficacy of temozolomide when used in combination therapy.
BRITISH JOURNAL OF CANCER
(2022)
Article
Multidisciplinary Sciences
Katia Chieregato, Martina Bernardi, Alberta Alghisi, Rosaria Giordano, Lorenza Lazzari, Omar Perbellini, Mario Rassu, Marco Ruggeri, Giuseppe Astori
Summary: Mesenchymal stromal cells (MSC) have potential in treating aGvHD and autoimmune disorders, but their recognition mechanisms are unclear and they are not completely immunoprivileged. Developing an in vitro test and a flow cytometric assay may be an easy method for predicting treatment response.
Article
Gastroenterology & Hepatology
Maurizio Marzaro, Mattia Algeri, Luigi Tomao, Stefano Tedesco, Tamara Caldaro, Valerio Balassone, Anna Chiara Contini, Luciano Guerra, Giovanni Federici D'Abriola, Paola Francalanci, Maria Emiliana Caristo, Lorenzo Lupoi, Ivo Boskoski, Angela Bozza, Giuseppe Astori, Gianantonio Pozzato, Alessandro Pozzato, Guido Costamagna, Luigi Dall'Oglio
THERAPEUTIC ADVANCES IN GASTROENTEROLOGY
(2020)