Article
Multidisciplinary Sciences
Tommi Kotila, Hugo Wioland, Muniyandi Selvaraj, Konstantin Kogan, Lina Antenucci, Antoine Jegou, Juha T. Huiskonen, Guillaume Romet-Lemonne, Pekka Lappalainen
Summary: The authors report the structure and function analysis of highly divergent actin from Leishmania parasite. The study reveals the rapid dynamics and molecular basis of parasite actin, providing insight into the evolution of the actin cytoskeleton. The findings show how divergent parasites achieve rapid actin dynamics using a simple set of actin-binding proteins, elucidating the evolution of the actin cytoskeleton.
NATURE COMMUNICATIONS
(2022)
Article
Agricultural Engineering
Zhi Deng, Xianghong Liu, Han Yin, Xiangyun Ji, Xiaoqing Ren, Zhihui Xia, Dejun Li
Summary: The actin cytoskeleton contributes to various cellular processes and actin-depolymerizing factor (ADF) is an important regulator of actin filament dynamics. This study investigates the role of HbADF4 in regulating actin dynamics in latex flow in rubber tree. The downregulation of HbADF4 resulted in an increase in the F/G-actin ratio in latex, while upregulation caused a decrease in the ratio. HbADF4 is also associated with stress and hormone responses in rubber tree and plays a role in cytokinesis and cell elongation.
INDUSTRIAL CROPS AND PRODUCTS
(2023)
Article
Biochemistry & Molecular Biology
Sharad V. Jaswandkar, Kalpana S. Katti, Dinesh R. Katti
Summary: This study investigates the impact of ADF/cofilin on actin filaments using molecular dynamics simulations. The results show that the binding of ADF/cofilin modifies the conformation and alignment of actin filaments locally, leading to filament severing at the boundaries. The findings provide important insights into the cofilin-mediated actin dynamics.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2022)
Article
Cell Biology
Shamulailatpam Shreedarshanee Devi, Rashmi Yadav, Ranjana Arya
Summary: Cell migration relies on coordinated cytoskeletal dynamics, reorganization, and signal transduction, with actin playing a central role. Abnormal sialylation can disrupt cell migration signaling, potentially contributing to cancer and myopathies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Plant Sciences
Yongduo Sun, Mengmeng Zhong, Yuanbao Li, Ruihui Zhang, Lei Su, Guixian Xia, Haiyun Wang
Summary: The study identified the involvement of the GhADF6 gene in cotton's response to the fungal pathogen Verticillium dahliae, showing that its down-regulation makes cotton plants tolerant to infection and leads to actin remodelling. This highlights the importance of actin depolymerizing factors in plant defense.
MOLECULAR PLANT PATHOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Hugo Wioland, Stephane Fremont, Berengere Guichard, Arnaud Echard, Antoine Jegou, Guillaume Romet-Lemonne
Summary: MICAL1-mediated oxidation of actin filaments amplifies cofilin severing action, bypassing the need for cofilin activation. Direct post-translational oxidation modification of actin filaments in cells triggers their disassembly.
Article
Multidisciplinary Sciences
Tomasz Zielinski, Joanna Pabijan, Bartlomiej Zapotoczny, Joanna Zemla, Julita Wesolowska, Joanna Pera, Malgorzata Lekka
Summary: In this study, the researchers used atomic force microscopy to measure the changes in biomechanical properties of neuroblastoma SH-SY5Y cells under oxygen and glucose deprivation and reoxygenation conditions. The results showed that the metabolic activity of the cells decreased with longer periods of oxygen and glucose deprivation, and the cells became softer. However, the mechanical properties of the cells recovered after reoxygenation. These changes in the nanomechanical properties were attributed to the remodelling of actin filaments.
SCIENTIFIC REPORTS
(2022)
Article
Biochemistry & Molecular Biology
Ruriko Suzuki, Yoshikazu Inoh, Satoru Yokawa, Tadahide Furuno, Naohide Hirashima
Summary: Aggregation of IgE bound to Fc epsilon RI induces mast cell activation, while disaggregation by monomer hapten terminates degranulation. Actin polymerization is involved in this process, with multivalent antigens depolymerizing actin filaments and monomer haptens recovering actin polymerization. Cofilin phosphorylation plays a key role in actin rearrangement and polymerization in mast cells.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Review
Cell Biology
James R. Bamburg, Laurie S. Minamide, O'Neil Wiggan, Lubna H. Tahtamouni, Thomas B. Kuhn
Summary: Proteins of the ADF/cofilin family are essential regulators of actin dynamics and function in eukaryotes. Cofilin-1 is the major isoform expressed in mammalian neurons, with ADF and cofilin-2 also present. Cofilin modulates actin transport and function, as well as plays a role in mitochondrial fission and mitophagy in neurons.
Article
Plant Sciences
Noriko Inada, Naoki Takahashi, Masaaki Umeda
Summary: The study found that specific genes in Arabidopsis thaliana can act as novel regulators of endoreplication, significantly affecting leaf area and ploidy level in cells.
JOURNAL OF PLANT RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Vibha Kaushik, Daniela Bruennert, Eva-Maria Hanschmann, Phulwanti Kumari Sharma, Bibin G. Anand, Karunakar Kar, Suneel Kateriya, Pankaj Goyal
Summary: The study found that cofilin-1 has an intrinsic tendency to aggregate and form amyloid-like structures in vitro. Oxidation at Cys-80 makes cofilin-1 unstable and leads to a partial loss of protein structure. The results support the hypothesis that cofilin-1 aggregation may play a role in cofilin-mediated pathology and the progression of several amyloid-linked diseases.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Genetics & Heredity
Sajal Kumar Halder, Md Oliullah Rafi, Esha Binte Shahriar, Sarah Albogami, Ahmed M. El-Shehawi, S. M. Muktasid Ud Daullah, Mahbubul Kabir Himel, Talha Bin Emran
Summary: This study provides a comprehensive understanding of the effects of single-nucleotide polymorphisms (SNPs) in the CFL1 gene on protein structure and function, suggesting that the L84P (rs199716082) and L99A (rs267603119) variants are significant CFL1 mutations associated with disease development.
Article
Multidisciplinary Sciences
Johnson Chung, Bruce L. Goode, Jeff Gelles
Summary: This study used single-molecule fluorescence imaging to observe the debranching process of actin networks by GMF and cofilin. The study found that cofilin, like GMF, is an authentic debrancher independent of its filament-severing activity and that the debranching activities of the two proteins are additive. Cofilin selectively accumulates on branch-junction daughter filaments, while GMF directly binds to the Arp2/3 complex. These observations suggest that GMF and cofilin promote debranching by distinct yet complementary mechanisms.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Immunology
ZhengQing Yu, LiXin Xu, Ke He, MingMin Lu, RuoFeng Yan, XiaoKai Song, XiangRui Li
Summary: The epidemic of avian coccidiosis seriously threatens the animals' welfare and the economic gains of the poultry industry. This study demonstrates that nanovaccines carrying recombinant E. mitis actin depolymerizing factor can effectively enhance immune protection against E. mitis infection in chickens.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Cell Biology
Juan Wang, Jiangfeng Shen, Yanan Xu, Yuxiang Jiang, Xiaolu Qu, Wanying Zhao, Yingjie Wang, Shanjin Huang
Summary: This study demonstrates that different pH sensitivities enable Arabidopsis ADF7 and ADF10 to regulate actin dynamics in response to cytosolic pH gradient, which supports pollen tube growth. ADF7 plays a dominant role in promoting actin turnover at the pollen tube apex, while ADF10 regulates the turnover and organization of subapical actin filaments. This functional specification and cooperation enable the coordination of the actin cytoskeleton with the cytosolic pH gradient in supporting pollen tube growth.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Cell Biology
Eichi Watabe, Shoichiro Ono, Hidehito Kuroyanagi
Article
Cell Biology
Hyunjung Lee, Suzanne G. Eskin, Shoichiro Ono, Cheng Zhu, Larry V. McIntire
JOURNAL OF CELL SCIENCE
(2019)
Article
Biochemistry & Molecular Biology
Kimihide Hayakawa, Carina Sekiguchi, Masahiro Sokabe, Shoichiro Ono, Hitoshi Tatsumi
JOURNAL OF MOLECULAR BIOLOGY
(2019)
Article
Biochemistry & Molecular Biology
Kanako Ono, Zhaozhao Qin, Robert C. Johnsen, David L. Baillie, Shoichiro Ono
Article
Multidisciplinary Sciences
Alicia Lardennois, Gabriella Pasti, Teresa Ferraro, Flora Llense, Pierre Mahou, Julien Pontabry, David Rodriguez, Samantha Kim, Shoichiro Ono, Emmanuel Beaurepaire, Christelle Gally, Michel Labouesse
Correction
Multidisciplinary Sciences
Alicia Lardennois, Gabriella Pasti, Teresa Ferraro, Flora Llense, Pierre Mahou, Julien Pontabry, David Rodriguez, Samantha Kim, Shoichiro Ono, Emmanuel Beaurepaire, Christelle Gally, Michel Labouesse
Correction
Multidisciplinary Sciences
Alicia Lardennois, Gabriella Pasti, Teresa Ferraro, Flora Llense, Pierre Mahou, Julien Pontabry, David Rodriguez, Samantha Kim, Shoichiro Ono, Emmanuel Beaurepaire, Christelle Gally, Michel Labouesse
Article
Biophysics
Zhenhai Li, Hyunjung Lee, Suzanne G. Eskin, Shoichiro Ono, Cheng Zhu, Larry McIntire
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Haitao Zhang, Auburn Ramsey, Yitong Xiao, Uddhab Karki, Jennifer Y. Xie, Jianfeng Xu, Thomas Kelly, Shoichiro Ono, Guo-Lei Zhou
MOLECULAR AND CELLULAR BIOLOGY
(2020)
Editorial Material
Multidisciplinary Sciences
Shoichiro Ono
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Shoichiro Ono, Kanako Ono
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Noriyuki Kodera, Hiroshi Abe, Phuong Doan N. Nguyen, Shoichiro Ono
Summary: CAP is a conserved actin-binding protein that regulates various aspects of actin dynamics; the XCAP1-actin complex isolated in this study had a unique structure and different stoichiometry compared to previous studies.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Cell Biology
Shoichiro Ono, Mario Lewis, Kanako Ono
Summary: This study demonstrates the mutual dependence of tropomodulin and tropomyosin in sarcomere assembly and regulation of actin filaments in the nematode Caenorhabditis elegans.
EUROPEAN JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Shoichiro Ono
Summary: The calponin family proteins, UNC-87 and CLIK-1, play both redundant and distinct roles in regulating actin filaments, with UNC-87 binding to myosin and inhibiting actomyosin ATPase, while CLIK-1 localizes near actin pointed ends.
Article
Cell Biology
Shoichiro Ono
Summary: The calponin family proteins play important roles in vertebrates, regulating actin-myosin interaction and actin filament stability, and are involved in muscle contraction and cell migration. These proteins have diversified in animals through gene amplification and repeat expansion of calponin-like motifs, resulting in variable molecular features.