Article
Optics
David R. Smith, Siddarth Shivkumar, Jeff Field, Jesse W. Wilson, Herve Rigneault, Randy A. Bartels
Summary: Impulsive stimulated Raman scattering (ISRS) is a powerful technique for studying low-frequency Raman vibrational modes, and this study introduces a simple strategy for ISRS spectroscopy and hyperspectral imaging using spectral filters.
Article
Chemistry, Analytical
Diwakar M. Awate, Cicero C. Pola, Erica Shumaker, Carmen L. Gomes, Jaime J. Juarez
Summary: Despite the limitations of flow cytometers, a 3D-printed imaging platform (3DPIP) has been developed to accurately count particles and conduct fluorescence microscopy efficiently. The 3DPIP utilizes a custom particle counter code and machine vision-based algorithm to identify and count particles, showing comparable accuracy to commercial particle counters. Additionally, the platform can perform static experiments and flow-through experiments effectively, making it a promising tool for use in resource-limited clinical environments.
Review
Immunology
Ryley D. McClelland, Tyce N. Culp, David J. Marchant
Summary: Viruses use host factors to enter cells and cause disease, and imaging techniques are crucial for studying virus entry and replication processes, providing insights into viral infection mechanisms. Quantifying viral images and using cryo-electron microscopy to gather structural information of viruses can help identify novel targets for antiviral therapeutics and vaccines.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Biochemical Research Methods
Dennis Wirth, Brook Byrd, Boyu Meng, Rendall R. Strawbridge, Kimberley S. Samkoe, Scott C. Davis
Summary: By utilizing spectral unmixing, the approach isolates the fluorophore of interest from autofluorescence and other fluorescent reporters, leading to improved detection limits, increased tumor contrast, and significant changes in image interpretation.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Immunology
Claudia Andrade, Barbara Oliveira, Silvia Guatelli, Pedro Martinez, Beatriz Simoes, Claudia Bispo, Cinzia Ferrario, Francesco Bonasoro, Jose Rino, Michela Sugni, Rui Gardner, Rita Zilhao, Ana Varela Coelho
Summary: In this study, coelomocytes present in the coelomic fluid of Marthasterias glacialis were characterized using a combination of flow cytometry, imaging flow cytometry, and fluorescence plus transmission electron microscopy. Two distinct coelomocyte populations were identified and studied in terms of abundance, morphology, ultrastructure, cell viability, and cell cycle profiles. The integrative experimental workflow allowed for a better understanding of the heterogeneity within these coelomocyte populations.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Chemistry, Analytical
Jost Stergar, Rok Hren, Matija Milanic
Summary: Hyperspectral microscope imaging (HMI) is a promising modality in histopathology that combines spatial information from standard microscopy and spectral-based contrast from hyperspectral imaging. This report describes the design, calibration, characterization, and validation of a custom-made laboratory HMI system, which shows comparable performance to classic spectrometry laboratory systems. The system is also validated against a laboratory hyperspectral imaging system for macroscopic samples, allowing for future comparison of spectral imaging results across different length scales.
Article
Nanoscience & Nanotechnology
Kirill Kniazev, Evgenii Zaitsev, Shubin Zhang, Yang Ding, Loc Ngo, Zhuoming Zhang, Gregory V. Hartland, Masaru Kuno
Summary: Label-free, bond-selective imaging has new opportunities in chemistry, biology, and material science. However, issues such as low sensitivity, low spatial and temporal resolution hinder its application in studying spatially-congested specimens. In this paper, a widefield infrared photothermal heterodyne imaging technique (wIR-PHI) is introduced, which overcomes these issues and enables high-resolution imaging with hyperspectral capabilities and high sensitivity. The technique has potential for kinetic imaging and spectroscopic studies in important chemical, biological, and material processes in the future. Rating: 9/10.
Article
Nanoscience & Nanotechnology
Xue Dong, Geng Tong, Xuankun Song, Xingchen Xiao, Yiting Yu
Summary: This study demonstrates a flexible multiline scanning hyperspectral microscopy system using a digital micromirror device (DMD) that can adjust the number of scanning lines for improved detection efficiency. Imaging experiments on various samples show the great potential of this system in cellular biology and material analysis. The on-chip scanning process also makes the system compact, lightweight, portable, and not constrained by location.
MICROSYSTEMS & NANOENGINEERING
(2021)
Article
Optics
Yingwen Zhang, Duncan England, Benjamin Sussman
Summary: This article presents a snapshot hyperspectral imaging technique that utilizes quantum ghost spectroscopy, allowing high spectral resolution without sacrificing spatial resolution and improving resource efficiency.
Article
Multidisciplinary Sciences
Allan S. Johnson, Jordi Valls Conesa, Luciana Vidas, Daniel Perez-Salinas, Christian M. Gunther, Bastian Pfau, Kent A. Hallman, Richard F. Haglund, Stefan Eisebitt, Simon Wall
Summary: Solid-state systems can host a variety of thermodynamic phases that are challenging to study on the nanoscale due to the coexistence of multiple phases. Coherent diffractive imaging spectroscopy (CDIS) enables nanometer-scale resolution hyperspectral imaging of vanadium oxide, extracting refractive indices of different phases from a single sample without evidence of correlation-driven phase transitions. CDIS allows quantitative full-field x-ray spectromicroscopy for studying phase separation in extreme sample environments.
Article
Materials Science, Multidisciplinary
D. J. Lahneman, M. M. Qazilbash
Summary: This research investigates the propagation of SPhP in STO in the far-infrared through midinfrared spectral range using broadband, near-field nanospectroscopy. Analytical modeling of experimental data reveals the dispersion characteristics of SPhPs in STO. This work establishes STO as a platform for perovskite-based broadband far-infrared and terahertz nanophotonics.
Article
Multidisciplinary Sciences
Ryo Kato, Toki Moriyama, Takayuki Umakoshi, Taka-aki Yano, Prabhat Verma
Summary: In this study, long-duration TERS imaging of a micrometer-sized WS2 sample was achieved in a reproducible manner, enabling the detection of nanoscale defects and rare optical signals in large area 2D materials.
Article
Optics
Jiarui Li, Hongyuan Zhang, Minjian Lu, Haoyun Wei, Yan Li
Summary: Impulsive stimulated Brillouin spectroscopy (ISBS) is a critical method for fast measurement of mechanical properties. Traditional data processing methods are inefficient in dealing with low signal-to-noise ratio (SNR) signals. In this study, an adaptive noise-suppression Matrix Pencil method is proposed to improve the sensitivity and speed of multi-component viscoelastic identification.
Article
Optics
Hang Yuan, Pengfei Zhang, Feng Gao
Summary: Compressive sensing has been introduced to accelerate data acquisition in confocal Raman imaging, with unsupervised compressive imaging methods able to maintain high spatial and spectral fidelity simultaneously, as well as smooth transitions at boundaries.
Article
Multidisciplinary Sciences
M. Gonzalez-Cabrera, K. Wieland, E. Eitenberger, A. Bleier, L. Brunnbauer, A. Limbeck, H. Hutter, C. Haisch, B. Lendl, A. Dominguez-Vidal, M. J. Ayora-Canada
Summary: This work presents a multisensor hyperspectral approach for the characterization of ultramarine blue, a valuable historical pigment, at the microscopic scale combining the information of four analytical techniques at the elemental and molecular levels. The hyperspectral images collected were combined in a single hypercube, where the pixels of the various spectral components are aligned on top of each other. Selected spectral descriptors have been defined to reduce data dimensionality before applying unsupervised chemometric data analysis approaches. Lazurite, responsible for the blue color of the pigment, was detected as the major mineral phase present in synthetic and good quality pigments. Impurities like pyrite were detected in lower quality samples, although the clear identification of other mineral phases with silicate basis was more difficult. There is no correlation between the spatial distribution of the bands arising in the Raman spectra of natural samples in the region 1200-1850 cm(-1) and any of the transition metals or rare earth elements (REE). With this information, the previous hypothesis (based on bulk analysis) attributing these bands to luminescence emissions due to impurities of these elements must be revised. We propose the consideration of CO2 molecules trapped in the cages of the aluminosilicate structure of sodalite-type. Additionally, correlation between certain Raman features and the combined presence of Ca, P, and REE, in particular Nd, was detected for the lowest quality pigment. Our results highlight the usefulness of fusing chemical images obtained via different imaging techniques to obtain relevant information on chemical structure and properties.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Chemical
Meagan A. Bunge, K. Neil Ruckart, Silas Leavesley, Gregory W. Peterson, Nien Nguyen, Kevin N. West, T. Grant Glover
INDUSTRIAL & ENGINEERING CHEMISTRY RESEARCH
(2015)
Editorial Material
Biochemical Research Methods
Silas J. Leavesley, Thomas C. Rich
Article
Biochemical Research Methods
Peter Favreau, Clarissa Hernandez, Ashley Stringfellow Lindsey, Diego F. Alvarez, Thomas Rich, Prashant Prabhat, Silas J. Leavesley
JOURNAL OF BIOMEDICAL OPTICS
(2014)
Article
Biochemical Research Methods
Peter F. Favreau, Clarissa Hernandez, Tiffany Heaster, Diego F. Alvarez, Thomas C. Rich, Prashant Prabhat, Silas J. Leavesley
JOURNAL OF BIOMEDICAL OPTICS
(2014)
Article
Multidisciplinary Sciences
Silas J. Leavesley, Whitley Ledkins, Petra Rocic
Article
Chemistry, Analytical
Naga S. Annamdevula, Brenner Sweat, Peter Favreau, Ashley S. Lindsey, Diego F. Alvarez, Thomas C. Rich, Silas J. Leavesley
Article
Biochemical Research Methods
Peter F. Favreau, Joshua A. Deal, Bradley Harris, David S. Weber, Thomas C. Rich, Silas J. Leavesley
JOURNAL OF BIOPHOTONICS
(2020)
Article
Cardiac & Cardiovascular Systems
Mikhail Alexeyev, Aron M. Geurts, Naga S. Annamdevula, C. Michael Francis, Silas Josiah Leavesley, Thomas C. Rich, Mark S. Taylor, Mike T. Lin, Ron Balczon, Jennifer Michelle Knighten, Diego F. Alvarez, Troy Stevens
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2020)
Article
Biochemical Research Methods
Craig M. Browning, Joshua Deal, Sam Mayes, Arslan Arshad, Thomas C. Rich, Silas J. Leavesley
Summary: Colorectal cancer ranks third in terms of both incidence and mortality rates. Early detection is crucial for positive treatment outcomes, and hyperspectral imaging (HSI) technology shows potential for enhancing early stage lesion detection. However, challenges remain in utilizing HSI technology in real-time endoscopy scenarios.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Oncology
Kevin J. Lee, Wen-Chi L. Chang, Xi Chen, Jacob Valiyaveettil, Veronica Ramirez-Alcantara, Elaine Gavin, Alla Musiyenko, Luciana Madeira da Silva, Naga S. Annamdevula, Silas J. Leavesley, Antonio Ward, Tyler Mattox, Ashley S. Lindsey, Joel Andrews, Bing Zhu, Charles Wood, Ashleigh Neese, Ashley Nguyen, Kristy Berry, Yulia Maxuitenko, Mary Pat Moyer, Elmar Nurmemmedov, Greg Gorman, Lori Coward, Gang Zhou, Adam B. Keeton, Harry S. Cooper, Margie L. Clapper, Gary A. Piazza
Summary: PDE10 is overexpressed in colon tumors, and inhibition by ADT 061 selectively suppresses colon cancer cell growth while activating cGMP/PKG signaling. ADT 061 is a novel PDE10 inhibitor that shows promising chemopreventive activity in a mouse model of colon cancer.
CANCER PREVENTION RESEARCH
(2021)
Article
Biochemical Research Methods
Craig M. Browning, Samantha Mayes, Samuel A. Mayes, Thomas C. Rich, Silas J. Leavesley
Summary: Spectroscopic image data has been used for molecular discrimination in various fields. Traditional spectral imaging techniques have trade-offs between acquisition time and spectral specificity. A new spectral imaging microscope platform using fluorescence excitation-scanning technology has been developed. By using a wavelength-specific LED array, the LED-based spectral illuminator provides spectral image data with comparable quality to other systems.
BIOMEDICAL OPTICS EXPRESS
(2022)
Article
Biotechnology & Applied Microbiology
Marina Parker, Naga S. Annamdevula, Donald Pleshinger, Zara Ijaz, Josephine Jalkh, Raymond Penn, Deepak Deshpande, Thomas C. Rich, Silas J. Leavesley
Summary: Hyperspectral imaging (HSI) technology has various applications in target detection and mixture analysis. This study focuses on the use of excitation-scanning HSI for dynamic cell signaling studies, specifically the study of Ca2+ signals in human airway smooth muscle cells (HASMCs). The results indicate that linear unmixing (LU) and matched filter (MF) analysis algorithms can effectively analyze the HSI data and extract quantitative signal traces. Additionally, pixel filtering based on a theoretical sensitivity framework can reveal subtle kinetic features. These findings suggest that excitation-scanning HSI has potential for kinetic measurements of cell signals and other dynamic cellular events.
BIOENGINEERING-BASEL
(2023)
Review
Social Sciences, Interdisciplinary
Craig M. Browning, Robert Cloutier, Thomas C. Rich, Silas J. Leavesley
Summary: Systems engineering is crucial for capturing customer desires and needs to conceptualize a system before physical development. This paper focuses on the endoscope system, analyzing its technological advancements and proposing improvements for better detection capabilities. The analysis suggests future endoscope systems will prioritize complex imaging and higher computational requirements to enhance optical diagnoses of early abnormal tissue growth.
Proceedings Paper
Engineering, Biomedical
Birsen Sirkeci-Mergen, Mallika Keralapura, Serena Coelho, Silas J. Leavesley, Thomas C. Rich
2013 IEEE 10TH INTERNATIONAL SYMPOSIUM ON BIOMEDICAL IMAGING (ISBI)
(2013)
Proceedings Paper
Microscopy
Peter F. Favreau, Thomas C. Rich, Prashant Prabhat, Silas J. Leavesley
THREE-DIMENSIONAL AND MULTIDIMENSIONAL MICROSCOPY: IMAGE ACQUISITION AND PROCESSING XX
(2013)