Cytometry of DNA replication and RNA synthesis: Historical perspective and recent advances based on “click chemistry”

Growth stimulation leads to cellular senescence when the cell cycle is blocked

(2010) Zoya N. Demidenko et al.   CELL CYCLE

Zinc fixation preserves flow cytometry scatter and fluorescence parameters and allows simultaneous analysis of DNA content and synthesis, and intracellular and surface epitopes

(2010) Uffe Birk Jensen et al.   CYTOMETRY PART A

New biomarkers probing depth of cell senescence assessed by laser scanning cytometry

(2010) Hong Zhao et al.   CYTOMETRY PART A

Rationale for the real-time and dynamic cell death assays using propidium iodide

(2010) Hong Zhao et al.   CYTOMETRY PART A

Laser scanning cytometry for automation of the micronucleus assay

(2010) Z. Darzynkiewicz et al.   MUTAGENESIS

Analysis of replication factories in human cells by super-resolution light microscopy

(2009) Zoltan Cseresnyes et al.   BMC CELL BIOLOGY

Click-iT™ assay with improved DNA distribution histograms

(2009) Ronald M. Hamelik et al.   CYTOMETRY PART A

Cell type specific applicability of 5-ethynyl-2′-deoxyuridine (EdU) for dynamic proliferation assessment in flow cytometry

(2009) Simone Diermeier-Daucher et al.   CYTOMETRY PART A

EdU incorporation is an alternative non-radioactive assay to [3H]thymidine uptake for in vitro measurement of mice T-cell proliferations

(2009) Yongmao Yu et al.   JOURNAL OF IMMUNOLOGICAL METHODS

5-Ethynyl-2′-deoxyuridine labeling detects proliferating cells in the regenerating avian cochlea

(2009) Christina L. Kaiser et al.   LARYNGOSCOPE

Cell cycle synchronization of Escherichia coli using the stringent response, with fluorescence labeling assays for DNA content and replication

(2009) Daniel J. Ferullo et al.   METHODS

Detection of S-phase cell cycle progression using 5-ethynyl-2′-deoxyuridine incorporation with click chemistry, an alternative to using 5-bromo-2′-deoxyuridine antibodies

(2008) Suzanne B. Buck et al.   BIOTECHNIQUES

Cu-Catalyzed Azide−Alkyne Cycloaddition

(2008) Morten Meldal et al.   CHEMICAL REVIEWS

A novel method based on click chemistry, which overcomes limitations of cell cycle analysis by classical determination of BrdU incorporation, allowing multiplex antibody staining

(2008) Paolo Cappella et al.   CYTOMETRY PART A

A chemical method for fast and sensitive detection of DNA synthesis in vivo

(2008) A. Salic et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA

Exploring RNA transcription and turnover in vivo by using click chemistry

(2008) C. Y. Jao et al.   PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA