Article
Cell Biology
Anna Ivanova, Olesya Gruzova, Elizaveta Ermolaeva, Olga Astakhova, Sheed Itaman, Grigori Enikolopov, Alexander Lazutkin
Summary: This study examines the effects of thymidine analogs on adult hippocampal neurogenesis and perinatal somatic development of mice. The results show that thymidine analogs can effectively label cells but may have long-term effects on cell survival. Additionally, higher doses of thymidine analogs adversely affect somatic maturation and brain growth in mouse pups.
Article
Biology
Amrita A. Iyer, Ishwar Hosamani, John D. Nguyen, Tiantian Cai, Sunita Singh, Melissa M. McGovern, Lisa Beyer, Hongyuan Zhang, Hsin- Jen, Rizwan Yousaf, Onur Birol, Jenny J. Sun, Russell S. Ray, Yehoash Raphael, Neil Segil, Andrew K. Groves, Brian S. Kim
Summary: Reprogramming of the cochlea with hair-cell-specific transcription factors such as ATOH1 has been proposed for treating hearing loss. However, the efficiency of hair cell reprogramming declines as the cochlea matures. A combination of ATOH1, GFI1, and POU4F3 can reprogram non-sensory cells of the cochlea to a hair cell fate, but the resulting cells are less mature compared to those generated at an earlier stage. Epigenetic accessibility and signaling from hair cells to supporting cells, including Notch signaling, are key factors that impede hair cell reprogramming in older animals.
Review
Biochemistry & Molecular Biology
Anna Ligasova, Ivo Frydrych, Karel Koberna
Summary: Cellular growth and preparation for division are collectively known as the cell cycle, which consists of several phases with different durations. The progression through these phases is regulated by both internal and external factors. Various methods, particularly those focusing on analyzing the duration of cell cycle phases, have been developed to study the role of these factors. This review aims to provide an overview of the basic methods for determining cell cycle phases and estimating their length, emphasizing on the effectiveness and reproducibility of these techniques.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Nathaniel T. Carpena, So-Young Chang, Celine D. G. Abueva, Jae Yun Jung, Min Young Lee
Summary: Studies have shown that co-culture or treatment with soluble factors from HEI-OC1 cells may promote the differentiation of ESCs, but there is currently insufficient evidence to prove the mature differentiation of inner ear hair cells.
SCIENTIFIC REPORTS
(2021)
Review
Neurosciences
Jieyu Qi, Wenjuan Huang, Yicheng Lu, Xuehan Yang, Yinyi Zhou, Tian Chen, Xiaohan Wang, Yafeng Yu, Jia-Qiang Sun, Renjie Chai
Summary: Hearing loss is a growing problem that burdens the global economy. Understanding the mechanisms of damage repair and promoting the regeneration of functional hair cells are urgent tasks in hearing research. This article reviews the mechanisms of hair cell reproduction, discusses therapeutic strategies for effective regeneration, and explores potential future approaches such as small molecule drugs and gene therapy.
NEUROSCIENCE BULLETIN
(2023)
Article
Multidisciplinary Sciences
John D. Nguyen, Juan Llamas, Tuo Shi, J. Gage Crump, Andrew K. Groves, Neil Segil
Summary: Mammalian hair cells cannot regenerate in adulthood, but can regenerate in embryonic and neonatal stages through the direct transdifferentiation of neighboring supporting cells. This regeneration potential is lost due to DNA methylation and chromatin accessibility changes in the supporting cells as they mature. Understanding these epigenetic modifications may provide insights for future hearing restoration therapies.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Biochemistry & Molecular Biology
Vikrant Rai, Shu Tu, Joseph R. Frank, Jian Zuo
Summary: Noise-induced, drug-related, and age-related disabling hearing loss is a global public health problem that affects approximately 466 million people. While non-mammalian vertebrates have the ability to regenerate sensory hair cells, this capacity is lost in mammals, leading to permanent hearing loss. Current treatments for hearing loss, such as cochlear implants and hearing devices, only provide limited help. Recent studies have shown promising results in regenerating mammalian cochlear hair cells by manipulating molecular pathways or transcription factors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Xiao-Jun Li, Charles Morgan, Prathamesh T. Nadar-Ponniah, Waldemar Kolanus, Angelika Doetzlhofer
Summary: Reactivation of LIN28B and TRIM71 enhances the regenerative potential of P5 cochlear supporting cells, allowing them to dedifferentiate and form progenitor-like cells, which leads to increased hair cell formation.
Article
Cell & Tissue Engineering
Hiroki Takeda, Anna Dondzillo, Jessica A. Randall, Samuel P. Gubbels
Summary: The study demonstrates the feasibility of human stem cell engraftment into an ablated mouse organ of Corti, showing increased engraftment and differentiation of transplanted cells into hair and supporting cell immunophenotypes after hair cell ablation prior to transplantation.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Genetics & Heredity
Marta Bialic, Baraah Al Ahmad Nachar, Maria Kozlak, Vincent Coulon, Etienne Schwob
Summary: This article describes a simple and robust method to measure the duration of the S phase in cell cultures. It utilizes a dual EdU-BrdU pulse-labeling regimen with incremental thymidine chases and quantification by flow cytometry. The method does not require cell synchronization or genome engineering and can be used for various types of cells.
Article
Neurosciences
Wenyan Li, Yizhou Quan, Mingqian Huang, Wei Wei, Yilai Shu, Huawei Li, Zheng-Yi Chen
Summary: The study describes a culture system for adult mammalian auditory system that overcomes the limitations posed by the tough bone encasement of the inner ear. The researchers use intact cochlear structures to maintain overall inner ear architecture and improve sensor epithelium survival in culture. They show successful transdifferentiation of adult supporting cells into hair cell-like cells and demonstrate their ability to attract adult ganglion neurites. The culture system holds potential for diverse studies including regeneration, hair cell-neuron pathways, and inner ear drug screening.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2022)
Article
Cell & Tissue Engineering
Dunia Abdul-Aziz, Nicolai Hathiramani, Lauren Phung, Vittoria Sykopetrites, Albert S. B. Edge
Summary: The transcriptional repressor HIC1 plays a crucial role in regulating Atoh1 expression in the cochlea, impacting hair cell differentiation in mice. Targeting HIC1 repression of Atoh1 may have implications for promoting hair cell regeneration.
Article
Multidisciplinary Sciences
Yi-Zhou Quan, Wei Wei, Volkan Ergin, Arun Prabhu Rameshbabu, Mingqian Huang, Chunjie Tian, Srinivas Vinod Saladi, Artur A. Indzhykulian, Zheng-Yi Chen
Summary: Strategies to overcome irreversible cochlear hair cell (HC) damage and loss in mammals are important for hearing recovery in patients with permanent hearing loss. Activation of Myc and Notch1 can promote HC regeneration and a combination of drug-like molecules has been identified to activate these pathways in mature nontransgenic cochlea.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2023)
Review
Cardiac & Cardiovascular Systems
John Auchampach, Lu Han, Guo N. Huang, Bernhard Kuhn, John W. Lough, Caitlin C. O'Meara, Alexander Y. Payumo, Nadia A. Rosenthal, Henry M. Sucov, Katherine E. Yutzey, Michaela Patterson
Summary: The field of mammalian myocardial regeneration has seen significant growth in the past two decades. However, there are limitations and challenges in characterizing cardiomyocyte proliferation. Claims of cardiomyocyte proliferation often lack sufficient evidence or proper controls, and the provided data may only indicate cell-cycle activation or DNA synthesis, without proving the generation of new cardiomyocytes. Rigorous experimental strategies and standards should be used to support claims of proliferation-based remuscularization.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Liyan Zhang, Yuan Fang, Fangzhi Tan, Fangfang Guo, Ziyu Zhang, Nianci Li, Qiuhan Sun, Jieyu Qi, Renjie Chai
Summary: This study reveals the essential role of the guanine nucleotide exchange factor Net1 in trans-differentiation of supporting cells (SCs) into hair cells (HCs). Overexpression of Net1 promotes cochlear organoid formation and HC differentiation. It also enhances SC proliferation and HC regeneration. These findings suggest that Net1 may serve as a potential target for HC regeneration and AAV-mediated gene regulation may be a promising approach for stem cell-based therapy in hearing restoration.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Endocrinology & Metabolism
Joseph F. Arboleda-Velasquez, Cammi N. Valdez, Christina K. Marko, Patricia A. D'Amore
CURRENT DIABETES REPORTS
(2015)
Article
Immunology
B. B. Menon, C. Kaiser-Marko, S. Spurr-Michaud, A. S. Tisdale, I. K. Gipson
MUCOSAL IMMUNOLOGY
(2015)
Article
Pathology
Christina K. Marko, Balaraj B. Menon, Gang Chen, Jeffrey A. Whitsett, Hans Clevers, Ilene K. Gipson
AMERICAN JOURNAL OF PATHOLOGY
(2013)
Review
Audiology & Speech-Language Pathology
Douglas A. Cotanche, Christina L. Kaiser
Article
Audiology & Speech-Language Pathology
Yohann Grondin, Douglas A. Cotanche, Otto Manneberg, Ramon Molina, J. Humberto Trevino-Villarreal, Rosalinda Sepulveda, Royce Clifford, Magda E. Bortoni, Scott Forsberg, Brian LaBrecque, Larisa Altshul, Joseph D. Brain, Ronald L. Jackson, Rick A. Rogers
Article
Cell Biology
J. Humberto Trevino-Villarreal, Douglas A. Cotanche, Rosalinda Sepulveda, Magda E. Bortoni, Otto Manneberg, Taturo Udagawa, Rick A. Rogers
JOURNAL OF HISTOCHEMISTRY & CYTOCHEMISTRY
(2011)
Article
Medicine, Research & Experimental
Matthew K. Lee, Christina L. Kaiser, Brittany J. Chapman, Douglas A. Cotanche
Article
Neurosciences
H. E. Karnes, C. L. Kaiser, D. Durham
Article
Multidisciplinary Sciences
Phillip M. Uribe, Melissa A. Mueller, Julia S. Gleichman, Matthew D. Kramer, Qi Wang, Martha Sibrian-Vazquez, Robert M. Strongin, Peter S. Steyger, Douglas A. Cotanche, Jonathan I. Matsui
Article
Multidisciplinary Sciences
Yohann Grondin, Magda E. Bortoni, Rosalinda Sepulveda, Elisa Ghelfi, Adam Bartos, Douglas Cotanche, Royce E. Clifford, Rick A. Rogers
Article
Ophthalmology
Dhanesh Amarnani, Arturo Israel Machuca-Parra, Lindsay L. Wong, Christina K. Marko, James A. Stefater, Tomasz P. Stryjewski, Dean Eliott, Joseph E. Arboleda-Velasquez, Leo A. Kim
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2017)
Article
Biochemistry & Molecular Biology
Leo A. Kim, Lindsay L. Wong, Dhanesh S. Amarnani, Alexander A. Bigger-Allen, Yang Hu, Christina K. Marko, Dean Eliott, Vinay A. Shah, Declan McGuone, Anat O. Stemmer-Rachamimov, Xiaowu Gai, Patricia A. D'Amore, Joseph F. Arboleda-Velasquez
Article
Ophthalmology
Christina Kaiser Marko, Ann S. Tisdale, Sandra Spurr-Michaud, Christopher Evans, Ilene K. Gipson
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2014)