Article
Multidisciplinary Sciences
S. S. Hoepner, Ana Raykova, R. Radpour, M. A. Amrein, D. Koller, G. M. Baerlocher, C. Riether, A. F. Ochsenbein
Summary: The study demonstrates that LIGHT and its receptor LT beta R promote quiescence and self-renewal of HSCs, while LT beta R deficiency contributes to survival in a mouse leukemia model.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Soo-Yeon Woo, Hansong Lee, Su Min Park, Hee-Seon Choi, Jayoung Kim, Munju Kwon, Jihyung Sohn, Ji Ho Nam, Hyung-Sik Kim, Parkyong Song, Ninib Baryawno, Yun-Hak Kim, Koanhoi Kim, Dongjun Lee
Summary: 27HC, an oxygenated derivative of cholesterol, regulates the quantity and function of various cell populations in the bone marrow and may serve as a new therapeutic target for hematological malignancies. Exogenous 27HC treatment induces oxidative stress and apoptosis in the bone marrow.
CELL DEATH & DISEASE
(2022)
Article
Medicine, Research & Experimental
Yihong Guan, Metis Hasipek, Dongxu Jiang, Anand D. Tiwari, Dale R. Grabowski, Simona Pagliuca, Sunisa Kongkiatkamon, Bhumika Patel, Salendra Singh, Yvonne Parker, Thomas LaFramboise, Daniel Lindner, Mikkael A. Sekeres, Omar Y. Mian, Yogen Saunthararajah, Jaroslaw P. Maciejewski, Babal K. Jha
Summary: Eltrombopag binds to the TET2 catalytic domain and inhibits its dioxygenase activity, leading to the expansion of normal hematopoietic stem cells and the prevention of neoplastic evolution in TET2 mutant malignant myeloid cells. This finding provides a scientific rationale for the treatment of myeloid malignancies associated with TET2 mutations or TET deficiency.
JOURNAL OF CLINICAL INVESTIGATION
(2022)
Review
Cell Biology
Bai Ling, Yunyang Xu, Siyuan Qian, Ze Xiang, Shihai Xuan, Jian Wu
Summary: Hematopoietic stem cells (HSCs) play a crucial role in the hematopoietic system as they can self-renew and differentiate into various blood cells. The mTOR signaling pathway is an important regulator of HSCs' differentiation, self-renewal, and quiescence, and several molecules can modulate these potentials by influencing this pathway. This review discusses the regulation of HSCs' three potentials by the mTOR signaling pathway and highlights potential regulators. Additionally, the clinical significance of studying this regulation and future predictions are also outlined.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Oncology
Isabella Maria Mayer, Andrea Hoelbl-Kovacic, Veronika Sexl, Eszter Doma
Summary: Transplantation of adult hematopoietic stem cells is crucial for treating hematological disorders, while leukemic stem cells play a significant role in disease initiation and relapse. Understanding methods to maintain and expand hematopoietic cells, as well as isolate and enrich hematopoietic stem cells and leukemic stem cells, is essential for developing therapeutic strategies.
Article
Medicine, Research & Experimental
Yandan Chen, Shuyi Fang, Qingwei Ding, Rongzhen Jiang, Jiefeng He, Qin Wang, Yuting Jin, Xinxin Huang, Sheng Liu, Maegan L. Capitano, Thao Trinh, Yincheng Teng, Qingyou Meng, Jun Wan, Hal E. Broxmeyer, Bin Guo
Summary: The study found that under ex vivo culturing conditions, HSCs with low levels of mitochondrial ROS were enriched in the CD34+CD133+ADGRG1+ CB cell population. These cells showed increased mitochondrial oxidative stress in transcriptomic and metabolic profiling, along with loss of stemness.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Cell & Tissue Engineering
Chuti Laowtammathron, Chanchao Lorthongpanich, Nittaya Jiamvoraphong, Pimonwan Srisook, Phatchanat Klaihmon, Pakpoom Kheolamai, Sudjit Luanpitpong, Surapol Issaragrisil
Summary: The study explores the role of YAP in hematopoietic differentiation of hiPSCs. It is found that suppressing YAP activity enhances the efficiency of hematopoietic differentiation and increases the yield of HSPCs.
STEM CELL RESEARCH & THERAPY
(2023)
Article
Biochemistry & Molecular Biology
James L. C. Che, Daniel Bode, Iwo Kucinski, Alyssa H. Cull, Fiona Bain, Hans J. Becker, Maria Jassinskaja, Melania Barile, Grace Boyd, Miriam Belmonte, Andy G. X. Zeng, Kyomi J. Igarashi, Juan Rubio-Lara, Mairi S. Shepherd, Anna Clay, John E. Dick, Adam C. Wilkinson, Hiromitsu Nakauchi, Satoshi Yamazaki, Berthold Gottgens, David G. Kent
Summary: The use of Fgd5 reporter mouse and EPCR surface marker allows for the exclusive identification of HSCs from non-HSCs in expansion cultures, revealing a gene expression signature that can identify functional HSCs from multiple cellular states.
Article
Multidisciplinary Sciences
Jacqueline Feyen, Zhen Ping, Lanpeng Chen, Claire van Dijk, Tim V. D. van Tienhoven, Paulina M. H. van Strien, Remco M. Hoogenboezem, Michiel J. W. Wevers, Mathijs A. Sanders, Ivo P. Touw, Marc H. G. P. Raaijmakers
Summary: Myeloid cells reduce the function of HSCs through interferon signaling via a neutrophil-NK cell dependent process.
NATURE COMMUNICATIONS
(2022)
Article
Cell & Tissue Engineering
Elia Henry, Frederic Picou, Vilma Barroca, Nathalie Dechamps, Steicy Sobrino, Emmanuelle Six, Camille Gobeaux, Patrick Auberger, Olivier Herault, Francoise Pflumio, Marie-Laure Arcangeli
Summary: In a steady state, hematopoietic stem cells (HSC) have low levels of reactive oxygen species (ROS). However, upon stress, HSC activate and their ROS levels increase, facilitating proliferation and differentiation. Antioxidants have been shown to maintain HSC self-renewal potential in various contexts, suggesting their potential use in culture-induced stress. This study demonstrates that the antioxidant Tempol can protect HSC functional properties during ex vivo culture, likely through VEGFa signaling.
STEM CELLS TRANSLATIONAL MEDICINE
(2023)
Article
Cell & Tissue Engineering
Han-Sol So, Min-Guk Kim, Jeong-Chae Lee, Sung-Ho Kook
Summary: The study found that using glucose oxidase (GO) can effectively promote the mobilization of hematopoietic stem progenitor cells (HSPCs) from the bone marrow to peripheral blood, even in animal models. Through combined use with granulocyte colony-stimulating factor (G-CSF) and/or AMD3100, the mobilization of HSPCs can be greatly enhanced.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Review
Cell Biology
Laura Crisafulli, Francesca Ficara
Summary: Hematopoietic stem cells (HSCs) play a crucial role in maintaining the homeostasis of the hematopoietic system, and micro-RNAs (miRNAs) serve as important regulators by fine-tuning gene expression to protect HSCs from unwanted expansion or aplasia.
WILEY INTERDISCIPLINARY REVIEWS-RNA
(2022)
Review
Genetics & Heredity
Qianru Zhan, Jing Wang, Heyang Zhang, Lijun Zhang
Summary: Hematopoietic stem cells have the potential to differentiate into various mature blood cells and possess certain biological properties such as quiescence, self-renewal, multilineage differentiation, and homing ability. The role of E3 ubiquitin ligases in the regulation of hematopoietic homeostasis and malignancies is gaining attention. This review focuses on the recent progress in understanding the crucial role of E3 ubiquitin ligases in regulating the biological properties of hematopoietic stem cells.
JOURNAL OF MOLECULAR MEDICINE-JMM
(2023)
Article
Medicine, General & Internal
Bijie Yang, Yuanyuan Liu, Feifei Xiao, Zhilong Liu, Zhe Chen, Zhigang Li, Chengfang Zhou, Mei Kuang, Yi Shu, Shan Liu, Lin Zou
Summary: Alkbh5 is crucial for reversing m(6)A modifications on mRNAs and plays a vital role in various physiological and pathological processes. Deletion of Alkbh5 leads to increased progenitor cell populations while compromising the self-renewal capacity of hematopoietic stem cells (HSCs). Alkbh5 may regulate hematopoiesis by reducing m(6)A modification of Cebpa and maintaining gene expression levels. This study unveils the essential role of Alkbh5 in regulating HSC homeostasis and provides a reference for future research in this area.
Article
Cell Biology
Thomas Bessy, Adrian Candelas, Benoit Souquet, Khansa Saadallah, Alexandre Schaeffer, Benoit Vianay, Damien Cuvelier, Samy Gobaa, Cecilia Nakid-Cordero, Julien Lion, Jean-Christophe Bories, Nuala Mooney, Thierry Jaffredo, Jerome Larghero, Laurent Blanchoin, Lionel Faivre, Stephane Brunet, Manuel Thery
Summary: The study reveals that the interaction between HSPCs and bone marrow stromal cells can induce polarization of HSPCs, leading to changes in cytoskeleton architecture, specifically the centrosome positioning. This polarization is specific and involves receptors ICAM, VCAM, and SDF1, with SDF1 being capable of independently inducing centrosome-microtubule network polarization.
JOURNAL OF CELL BIOLOGY
(2021)
Article
Neurosciences
Paolo Barbaresi, Emanuela Mensa, Andrea Sagrati, Laura Graciotti
NEUROSCIENCE RESEARCH
(2020)
Article
Oncology
Julie A. Hixon, Caroline Andrews, Lila Kashi, Casey L. Kohnhorst, Emilee Senkevitch, Kelli Czarra, Joao T. Barata, Wenqing Li, Joel P. Schneider, Scott T. R. Walsh, Scott K. Durum
Article
Cell Biology
Emanuela Mensa, Michele Guescini, Angelica Giuliani, Maria Giulia Bacalini, Deborah Ramini, Giacomo Corleone, Manuela Ferracin, Gianluca Fulgenzi, Laura Graciotti, Francesco Prattichizzo, Leonardo Sorci, Michela Battistelli, Vladia Monsurro, Anna Rita Bonfigli, Maurizio Cardelli, Rina Recchioni, Fiorella Marcheselli, Silvia Latini, Serena Maggio, Mirco Fanelli, Stefano Amatori, Gianluca Storci, Antonio Ceriello, Vilberto Stocchi, Maria De Luca, Luca Magnani, Maria Rita Rippo, Antonio Domenico Procopio, Claudia Sala, Iva Budimir, Cristian Bassi, Massimo Negrini, Paolo Garagnani, Claudio Franceschi, Jacopo Sabbatinelli, Massimiliano Bonafe, Fabiola Olivieri
JOURNAL OF EXTRACELLULAR VESICLES
(2020)
Article
Endocrinology & Metabolism
Francesco Prattichizzo, Angelica Giuliani, Jacopo Sabbatinelli, Giulia Matacchione, Deborah Ramini, Anna Rita Bonfigli, Maria Rita Rippo, Paola de Candia, Antonio Domenico Procopio, Fabiola Olivieri, Antonio Ceriello
DIABETES OBESITY & METABOLISM
(2020)
Article
Immunology
Felix E. Y. Aggor, Timothy J. Break, Giraldina Trevejo-Nunez, Natasha Whibley, Bianca M. Coleman, Rachel D. Bailey, Daniel H. Kaplan, Julian R. Naglik, Wei Shan, Amol C. Shetty, Carrie McCracken, Scott K. Durum, Partha S. Biswas, Vincent M. Bruno, Jay K. Kolls, Michail S. Lionakis, Sarah L. Gaffen
SCIENCE IMMUNOLOGY
(2020)
Article
Endocrinology & Metabolism
Francesco Prattichizzo, Valeria De Nigris, Jacopo Sabbatinelli, Angelica Giuliani, Carlos Castano, Marcelina Parrizas, Isabel Crespo, Annalisa Grimaldi, Nicolo Baranzini, Rosangela Spiga, Elettra Mancuso, Maria Rita Rippo, Antonio Domenico Procopio, Anna Novials, Anna Rita Bonfigli, Silvia Garavelli, Lucia La Sala, Giuseppe Matarese, Paola de Candia, Fabiola Olivieri, Antonio Ceriello
Summary: The study introduced a new method to isolate plasma CD31(+) EVs and identified certain miRNAs associated with vascular performance in T2DM patients, suggesting their potential role in T2DM and complications.
Article
Cell Biology
Jacopo Sabbatinelli, Angelica Giuliani, Giulia Matacchione, Silvia Latini, Noemi Laprovitera, Giovanni Pomponio, Alessia Ferrarini, Silvia Svegliati Baroni, Marianna Pavani, Marco Moretti, Armando Gabrielli, Antonio Domenico Procopio, Manuela Ferracin, Massimiliano Bonafe, Fabiola Olivieri
Summary: The expression levels of miR-146a-5p related to inflammaging changed in COVID-19 patients after receiving treatment with Tocilizumab, with patients who did not respond to the drug having lower levels of miR-146a-5p and experiencing worse outcomes. This blood-based biomarker may offer insights into the molecular link between inflammaging and the clinical course of COVID-19, providing a better understanding for using biologic drugs against this global health threat.
MECHANISMS OF AGEING AND DEVELOPMENT
(2021)
Article
Biochemistry & Molecular Biology
Angelica Giuliani, Alberto Montesanto, Giulia Matacchione, Laura Graciotti, Deborah Ramini, Olga Protic, Roberta Galeazzi, Roberto Antonicelli, Elena Tortato, Anna Rita Bonfigli, Jacopo Sabbatinelli, Fabiola Olivieri
Summary: Disorders of lipoprotein metabolism are associated with cardiovascular disease development. Certain SNPs in coding genes are associated with individual lipid profiles and response to lipid-lowering treatments. The rs3746444 SNP in the MIR499 gene is associated with a favorable blood lipid profile.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Nicolas Serafini, Angelique Jarade, Laura Surace, Pedro Goncalves, Odile Sismeiro, Hugo Varet, Rachel Legendre, Jean-Yves Coppee, Olivier Disson, Scott K. Durum, Gad Frankel, James P. Di Santo
Summary: Group 3 innate lymphoid cells (ILC3s) in the intestine remain activated for months after exposure to Citrobacter rodentium, leading to enhanced immune response and defense capability. These trained ILC3s undergo metabolic changes and exhibit enhanced proliferative capacity and IL-22 production.
Article
Oncology
Federica Monaco, Laura De Conti, Simone Vodret, Nunzia Zanotta, Manola Comar, Sandra Manzotti, Corrado Rubini, Laura Graciotti, Gianluca Fulgenzi, Massimo Bovenzi, Marco Baralle, Marco Tomasetti, Lory Santarelli
Summary: This study proposes a novel miRNA-based therapy approach to target malignant pleural mesothelioma (MPM) by increasing stemness and inhibiting cell proliferation. It is found that by inhibiting the release of exosomes, miR-126 can accumulate intracellularly and induce cell death and tumor growth arrest. Autophagy and necroptosis are involved in these processes, suggesting the potential of this therapy against cancer stem cells (CSCs).
TRANSLATIONAL ONCOLOGY
(2022)
Article
Biology
Daniel P. Brice, Graeme Murray, Heather M. Wilson, Ross J. Porter, Susan Berry, Scott K. Durum, Mairi H. McLean
Summary: This study demonstrates that interleukin-27 (IL-27) plays a crucial role in the function of gut epithelial barrier, restoring permeability, regulating immune responses, and promoting wound healing, suggesting its potential as a treatment strategy for inflammatory bowel disease (IBD).
Biographical-Item
Immunology
Scott K. Durum
Review
Biochemistry & Molecular Biology
Hila Winer, Gisele O. L. Rodrigues, Julie A. Hixon, Francesca B. Aiello, Tu Chun Hsu, Brianna T. Wachter, Wenqing Li, Scott K. Durum
Summary: IL-7 is a cytokine that plays a crucial role in the development and survival of lymphoid cells. It regulates cell functions through the IL-7 receptor signal transduction pathway. Abnormalities in the IL-7 pathway can lead to immunodeficiencies and autoimmune diseases. IL-7 has potential clinical applications in cancer treatment and autoimmune diseases.
Article
Pharmacology & Pharmacy
M. Pesaresi, F. Pirani, A. Tagliabracci, M. Valsecchi, A. D. Procopio, F. P. Busardo, L. Graciotti
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2020)