Review
Plant Sciences
Zhanru Shao, Delin Duan
Summary: Cell wall polysaccharides (CWPS) of seaweeds play crucial roles in various applications and interactions in marine environments. Recent studies have advanced the understanding of CWPS biosynthesis pathways, gene expression levels, and potential regulation mechanisms. However, further research is needed to fully comprehend the relationship between the chemical structure and bioactivities of CWPS, as well as develop effective gene editing tools.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Jordan D. Julian, Olga A. Zabotina
Summary: This review discusses the composition of plant cell walls and the biosynthesis process of xyloglucan. It focuses on the research of xyloglucan in Arabidopsis thaliana, Oryza sativa, and Solanum lycopersicum, and also explores the recent advances in understanding the glycosyltransferases involved in this complex process.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Plant Sciences
Olga A. Zabotina, Ning Zang, Richard Weerts
Summary: Glycosyltransferases (GTs) are enzymes that attach activated sugar to acceptor substrates. While progress has been made in cloning genes encoding polysaccharide-synthesizing GTs, the majority of GTs remain uncharacterized. The mechanism by which they synthesize complex polysaccharide structures in the Golgi membrane is largely unknown.
FRONTIERS IN PLANT SCIENCE
(2021)
Review
Plant Sciences
Heather E. McFarlane
Summary: Plant cell walls, composed of polysaccharides, provide support and enable growth. Recent research has made significant progress in understanding cell wall synthesis, including the identification and study of enzymes, the development of tools, and structural information generation. However, many questions about plant cell wall polysaccharide synthesis remain unanswered. This article discusses these questions, reviews supporting data, and explores potential technological advancements for future answers.
JOURNAL OF EXPERIMENTAL BOTANY
(2023)
Review
Cell Biology
Natalie Hoffmann, Samuel King, A. Lacey Samuels, Heather E. McFarlane
Summary: The composition and synthesis of the cell wall vary among different plant species, cell types, and regions within a cell wall, but are underpinned by common cellular mechanisms.
DEVELOPMENTAL CELL
(2021)
Article
Plant Sciences
Jianchao Yan, Jiandong Zhu, Jun Zhou, Chenghua Xing, Hongming Song, Kun Wu, Miaozhen Cai
Summary: The components and structure of the cell wall play a crucial role in aluminum toxicity and tolerance in plants. This study investigated the effects of Brefeldin A (BFA) and nitric oxide (NO) on cell wall assembly and function in response to aluminum. The results showed that BFA inhibited cell wall biosynthesis and made the root cell wall lose its ability to resist aluminum, while NO modified the cell wall composition and functional groups. These findings provide important insights into the mechanisms underlying aluminum tolerance in rice.
FRONTIERS IN PLANT SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Baocai Zhang, Yihong Gao, Lanjun Zhang, Yihua Zhou
Summary: The plant cell wall, composed of multiple biopolymers, is one of the most complex structural networks in nature. Through advancements in plant functional genomics, significant progress has been made in understanding cell wall biosynthesis, construction, and functions, as well as utilization of cell wall materials. Cutting-edge technologies have provided new insights into the intricate nanoscale network of the plant cell wall, opening up unprecedented possibilities for research.
JOURNAL OF INTEGRATIVE PLANT BIOLOGY
(2021)
Article
Microbiology
Joana Figueiredo, Mafalda Xavier Henriques, Maria Joao Catalao, Sara Pinheiro, Ana Rita Narciso, Francisco Mesquita, Bruno Manuel Saraiva, Madalena Carido, Didier Cabanes, Mariana Gomes Pinho, Sergio Raposo Filipe
Summary: This study identified a protein that regulates the synthesis of capsular polysaccharides in Streptococcus pneumoniae and determined its mechanism of directing the protein to the division septum of bacteria. These findings are important for understanding bacterial propagation and for the design of anti-infective strategies.
Review
Chemistry, Multidisciplinary
Jackson K. B. Cahn, Jorn Piel
Summary: This review discusses available and emerging techniques for targeted single-cell analysis, with a particular focus on applications to the discovery and study of natural products.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Engineering, Environmental
Xuan Nie, Fan Gao, Wei You, Guang Chen, Qi Shao, Long-Hai Wang, Wei-Qiang Huang, Lei Xia, Ze Zhang, Chun-Yan Hong, Ye-Zi You
Summary: The increasing spread of antimicrobial resistance calls for the urgent need to find new antibiotics that kill bacteria through a novel mechanism to avoid the development of resistance. Researchers have discovered a synthetic cage that tightly surrounds pyrophosphate structures, effectively killing bacteria and preventing the emergence of resistance.
CHEMICAL ENGINEERING JOURNAL
(2022)
Review
Biochemistry & Molecular Biology
Colin Ruprecht, Markus Blaukopf, Fabian Pfrengle
Summary: A sustainable bioeconomy can meet the growing global demands for food, energy and materials through increased agricultural productivity and the use of renewable biomass. Understanding the structure and biosynthesis of plant cell wall glycans is crucial for optimal utilization, but current research is hindered by a lack of available samples. This article provides an update on the chemical synthesis of plant cell wall oligosaccharides and their application in characterizing cell wall-directed antibodies and carbohydrate-active enzymes.
CURRENT OPINION IN CHEMICAL BIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Sujeet Kumar, Aurelio Mollo, Daniel Kahne, Natividad Ruiz
Summary: This review summarizes recent major discoveries in peptidoglycan (PG) biogenesis, with a focus on the translocation of Lipid II and the SEDs glycosyltransferases. These findings may lead to the development of new antibiotics to combat bacterial resistance.
Article
Plant Sciences
Debora Pagliuso, Adriana Grandis, Cristiane Ribeiro de Sousa, Amanda Pereira de Souza, Carlos Driemeier, Marcos S. Buckeridge
Summary: Cellulosic ethanol is an alternative to increase bioethanol production worldwide. The degradation of sugarcane straw in the field showed significant changes in the composition of carbohydrates and lignin, with rapid degradation of non-structural carbohydrates and slower degradation of cellulose. The data suggest that for soil mineralization, sugarcane straw should stay in the field for over a year, while for bioenergy purposes, it should be utilized within 3 months.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Plant Sciences
Margalida Roig-Oliver, Cyril Douthe, Josefina Bota, Jaume Flexas
Summary: The study investigated the influence of cell wall thickness and composition on photosynthesis in mosses, revealing variations in photosynthetic rates among different species and suggesting that cell wall composition may affect both cell wall thickness and mesophyll conductance.
PHYSIOLOGIA PLANTARUM
(2021)
Article
Plant Sciences
Hannah M. Tetreault, Tammy Gries, Sarah Liu, John Toy, Zhanguo Xin, Wilfred Vermerris, John Ralph, Deanna L. Funnell-Harris, Scott E. Sattler
Summary: The Bmr30 gene mutation results in significantly reduced CHI activity, impaired accumulation of total flavonoids and total anthocyanins. In the bmr30 mutants, the level of the flavone lignin monomer tricin was reduced 20-fold in the stem relative to wild type, and to undetectable levels in the leaf tissue of the mutants.
FRONTIERS IN PLANT SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Nicolas Cifuentes-Esquivel, Jonathan Celiz-Balboa, Carlos Henriquez-Valencia, Irina Mitina, Paulina Arrano-Salinas, Adrian A. Moreno, Claudio Meneses, Francisca Blanco-Herrera, Ariel Orellana
JOURNAL OF CELLULAR BIOCHEMISTRY
(2018)
Article
Plant Sciences
Ricardo Nilo-Poyanco, Paula Vizoso, Dayan Sanhueza, Ivan Balic, Claudio Meneses, Ariel Orellana, Reinaldo Campos-Vargas
PHYSIOLOGIA PLANTARUM
(2019)
Article
Agriculture, Multidisciplinary
Talia del Pozo, Simon Miranda, Mauricio Latorre, Felipe Olivares, Leonardo Pavez, Ricardo Gutierrez, Jonathan Maldonado, Patricio Hinrichsen, Bruno G. Defilippi, Ariel Orellana, Mauricio Gonzalez
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2019)
Article
Multidisciplinary Sciences
Elena A. Vidal, Tomas C. Moyano, Bernabe Bustos, Eduardo Perez-Palma, Carol Moraga, Eleodoro Riveras, Alejandro Montecinos, Lorena Azocar, Daniela C. Soto, Mabel Vidal, Alex Di Genoval, Klaus Puschel, Peter Nurnberg, Stephan Buch, Jochen Hampe, Miguel L. Allende, Veronica Cambiazo, Mauricio Gonzalez, Christian Hodar, Martin Montecino, Claudia Munoz-Espinoza, Ariel Orellana, Angelica Reyes-Jara, Dante Travisanyl, Paula Vizoso, Mauricio Moraga, Susana Eyheramendy, Alejandro Maass, Giancarlo De Ferrari, Juan Francisco Mique, Rodrigo A. Gutierrez
SCIENTIFIC REPORTS
(2019)
Article
Plant Sciences
Juan Pablo Parra-Rojas, Asier Largo-Gosens, Tomas Carrasco, Jonathan Celiz-Balboa, Veronica Arenas-Morales, Pablo Sepulveda-Orellana, Henry Temple, Dayan Sanhueza, Francisca C. Reyes, Claudio Meneses, Susana Saez-Aguayo, Ariel Orellana
JOURNAL OF EXPERIMENTAL BOTANY
(2019)
Article
Plant Sciences
Claudia Munoz-Espinoza, Alex Di Genova, Alicia Sanchez, Jose Correa, Alonso Espinoza, Claudio Meneses, Alejandro Maass, Ariel Orellana, Patricio Hinrichsen
Article
Biotechnology & Applied Microbiology
Ricardo Nilo-Poyanco, Carol Moraga, Gianfranco Benedetto, Ariel Orellana, Andrea Miyasaka Almeida
Summary: Shotgun proteomics is an unbiased approach to study the proteome of mature and ripe fruit in Prunus persica, revealing critical molecular processes involved in fruit ripening. Analysis showed that about 15% of proteins identified were modulated by ripening, with processes such as glycogen and isocitrate metabolism, protein localization, and cell wall modification being overrepresented. Additionally, potential biomarkers for ripening included proteins involved in pectin metabolism, hormone synthesis and transcription factor regulation.
Article
Plant Sciences
Jonathan Celiz-Balboa, Asier Largo-Gosens, Juan Pablo Parra-Rojas, Veronica Arenas-Morales, Pablo Sepulveda-Orellana, Hernan Salinas-Grenet, Susana Saez-Aguayo, Ariel Orellana
FRONTIERS IN PLANT SCIENCE
(2020)
Article
Multidisciplinary Sciences
Claudio Olmos, Gonzalo Campana, Victor Monreal, Paola Pidal, Nannet Sanchez, Constanza Airola, Dayan Sanhueza, Patricio Tapia, Ana Maria Munoz, Felipe Corvalan, Sebastian Hurtado, Claudio Meneses, Ariel Orellana, Martin Montecino, Gloria Arriagada, Fernando Jose Bustos
Summary: Asymptomatic SARS-CoV-2 infection was detected in 3.14% of healthcare workers in a private clinic in Santiago, Chile. Most of the infected individuals did not show symptoms within 3 weeks of testing, and genetic analysis suggested community infection as the main source of transmission among healthcare workers. The study indicated that the protocols implemented in the clinic were effective in preventing nosocomial infection clusters caused by asymptomatic carriers.
Article
Biotechnology & Applied Microbiology
Alex Di Genova, Gino Nardocci, Rodrigo Maldonado-Agurto, Christian Hodar, Camilo Valdivieso, Pamela Morales, Felipe Gajardo, Raquel Marina, Rodrigo A. Gutierrez, Ariel Orellana, Veronica Cambiazo, Mauricio Gonzalez, Alvaro Glavic, Marco A. Mendez, Alejandro Maass, Miguel L. Allende, Martin A. Montecino
Summary: The study on Orestias ascotanensis from the Chilean Altiplano reveals a genomic evolution process involving gene contraction and expansion, particularly in genes related to genome stability and DNA repair under positive selection to cope with high-altitude aquatic environments.
Article
Immunology
Paola Pidal, Jorge Fernandez, Constanza Airola, Miguel Araujo, Ana Maria Menjiba, Hector San Martin, Nicole Bruneau, Monserrat Balanda, Coral Elgueta, Rodrigo Fasce, Maria Teresa Valenzuela, Ariel Orellana, Eugenio Ramirez
Summary: Understanding the host immune response to SARS-CoV-2 variants of concern is crucial for improving diagnostics, therapy development, and vaccines. This study analyzed the level of neutralizing antibodies against different variants in healthcare workers previously infected with the D614G variant. The results showed that neutralizing antibody levels varied among different variants and changed over time during the follow-up period. There was evidence of immune escape from neutralizing antibodies with the Omicron variant due to mutations in the S protein.
MEDICAL MICROBIOLOGY AND IMMUNOLOGY
(2023)
Article
Plant Sciences
Henry Temple, Pyae Phyo, Weibing Yang, Jan J. Lyczakowski, Alberto Echevarria-Poza, Igor Yakunin, Juan Pablo Parra-Rojas, Oliver M. Terrett, Susana Saez-Aguayo, Ray Dupree, Ariel Orellana, Mei Hong, Paul Dupree
Summary: Polysaccharide methylation, particularly of pectin, is a crucial feature of land plant cell walls. This study identifies putative Golgi SAM transporters in Arabidopsis and shows their significance in the import of SAM molecules for polysaccharide methylation in the Golgi, ultimately affecting cell wall structure and function.
Article
Multidisciplinary Sciences
Gil Eshel, Viviana Araus, Soledad Undurraga, Daniela C. Soto, Carol Moraga, Alejandro Montecinos, Tomas Moyano, Jonathan Maldonado, Francisca P. Diaz, Kranthi Varala, Chase W. Nelson, Orlando Contreras-Lopez, Henrietta Pal-Gabor, Tatiana Kraiser, Gabriela Carrasco-Puga, Ricardo Nilo-Poyanco, Charles M. Zegar, Ariel Orellana, Martin Montecino, Alejandro Maass, Miguel L. Allende, Robert DeSalle, Dennis W. Stevenson, Mauricio Gonzalez, Claudio Latorre, Gloria M. Coruzzi, Rodrigo A. Gutierrez
Summary: The Atacama Desert in Chile is a harsh environment with extreme conditions, yet it supports a diverse range of plant species including endemic plants. Research conducted in the region has identified genes associated with plant adaptation to stressful environments, including those linked to stress responses, metabolism, and energy production. This genetic information could be valuable for engineering crop resilience to climate change, especially as some Atacama plants are closely related to staple crops.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Susana Saez-Aguayo, Juan Pablo Parra-Rojas, Pablo Sepulveda-Orellana, Jonathan Celiz-Balboa, Veronica Arenas-Morales, Christine Salle, Hernan Salinas-Grenet, Asier Largo-Gosens, Helen M. North, Marie-Christine Ralet, Ariel Orellana
Summary: The biosynthesis of Rhamnogalacturonan-I involves transport of UDP-Rhamnose into the Golgi apparatus by URGT transporters. URGT2, URGT4, and URGT6 play distinct roles in mucilage biosynthesis, with URGT2 and URGT4 mainly involved in RG-I elongation and URGT6 affecting adherent mucilage. Mutants lacking all three transporters show changes in polymer chains and xylan production.
Meeting Abstract
Chemistry, Multidisciplinary
Marie-Christine Ralet, Susana Saez-Aguayo, Ariel Orellana, Helen North
ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY
(2019)
