Article
Health Care Sciences & Services
Deni Ramljak, Martina Vukoja, Marina Curlin, Katarina Vukojevic, Maja Barbaric, Una Glamoclija, Bejana Purisevic, Olivera Peric, Violeta Soljic
Summary: The study suggests that CD8+ T cells with higher perforin-1 and lower Fas ligand expression in the early stages of COVID-19 infection may be associated with progression to moderate and severe forms of the disease later on. Additionally, the frequency of effector memory CD8+ T cells was lower in all COVID-19 patients, and the mRNA expression of perforin-1 and interferon-gamma were significantly downregulated.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Genetics & Heredity
Jan Futas, Jan Oppelt, Pamela Anna Burger, Petr Horin
Summary: Through bioinformatic analysis and re-sequencing of camelid genomes, functional genes encoding perforin, granulysin, and granzymes were identified in all Old World camelids, with some interspecific differences observed in the number of loci for certain genes such as GZMH. High protein sequence similarities were found among camelid and ungulate species for certain granzymes, while overall protein variability was relatively low in dromedaries and Bactrian camels.
Article
Microbiology
David D. Feehan, Khusraw Jamil, Maria J. Polyak, Henry Ogbomo, Mark Hasell, Shu Shun Li, Richard F. Xiang, Michael Parkins, Joseph A. Trapani, Joe J. Harrison, Christopher H. Mody
Summary: Pseudomonas aeruginosa is an opportunistic pathogen that infects cystic fibrosis patients. This study found that natural killer cells kill P. aeruginosa through a contact-dependent process that involves membrane damage, and granzyme-induced ROS production is redundant in this process.
Article
Biochemistry & Molecular Biology
Takahiro Uchida, Muneharu Yamada, Dan Inoue, Tadasu Kojima, Noriko Yoshikawa, Shingo Suda, Hidenobu Kamohara, Takashi Oda
Summary: A retrospective evaluation of SA-AKI patients showed that CD56(+) T cells are involved in the disease processes of SA-AKI through effector molecules such as FasL or perforin. However, the antigen-presenting capacity and phagocytic activity of monocytes in patients were significantly decreased and did not recover soon after the initiation of CRRT.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biology
Nora Trinks, Sebastian Reinhard, Matthias Drobny, Linda Heilig, Jurgen Loffler, Markus Sauer, Ulrich Terpitz
Summary: The study introduces an ExM method that allows super-resolved visualization of the interaction between NK cells and Aspergillus fumigatus hyphae. 4-fold expansion combined with confocal fluorescence imaging reveals details of cytoskeleton rearrangement and NK cells' lytic granules triggered by contact with an RFP-expressing A. fumigatus strain.
COMMUNICATIONS BIOLOGY
(2021)
Article
Medicine, Research & Experimental
Marijana Vicic, Vlatka Sotosek, Ines Brajac, Marija Kastelan, Larisa Prpic-Massari
Summary: Lichen planus is a chronic mucocutanous disorder where cytotoxic lymphocytes attack basal keratinocytes leading to apoptosis. The article hypothesizes that granulysin molecule may play a crucial role in keratinocytes killing in lichen planus.
MEDICAL HYPOTHESES
(2021)
Review
Immunology
Lucia Martinez Cuesta, Sandra Elizabeth Perez
Summary: Perforin and granzymes are crucial components in cytotoxic T lymphocytes and natural killer cells, playing key roles in viral infections, tumor immune surveillance, and tissue rejection. However, they have also been associated with tissue damage and immune diseases, with their release during viral infections potentially contributing to disease pathogenesis. This review provides insights into the impact of perforin and granzymes on neurological infections caused by bovine herpesviruses.
COMPARATIVE IMMUNOLOGY MICROBIOLOGY AND INFECTIOUS DISEASES
(2021)
Article
Biology
Violeta Soljic, Maja Barbaric, Martina Vukoja, Marina Curlin, Martina Orlovic Vlaho, Edita Cerni Obrdalj, Lidija Lasic Arapovic, Daniela Bevanda Glibo, Katarina Vukojevic
Summary: The study indicates that the expression of cytotoxic CD8(+) T cells in decidual tissue of patients with preeclampsia is altered, suggesting their potential role in the pathophysiology of preeclampsia and fetal-maternal immune tolerance.
Article
Medicine, Research & Experimental
Shizuka Otsuka, Nicolas Melis, Matthias M. Gaida, Debjani Dutta, Roberto Weigert, Jonathan D. Ashwell
Summary: Inhibitors of calcineurin phosphatase activity are commonly used to treat transplant rejection and graft-versus-host disease by inhibiting NFAT-dependent gene expression, but they may also suppress TCR-proximal signaling by preventing Lck(S59) dephosphorylation. Studies have shown that calcineurin inhibitors are ineffective in treating disease when T cells express Lck(S59A), suggesting that targeting TCR-associated calcineurin directly could provide more effective therapies with less toxicity.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Review
Immunology
Ariel Ramirez-Labrada, Cecilia Pesini, Llipsy Santiago, Sandra Hidalgo, Adanays Calvo-Perez, Carmen Onate, Alejandro Andres-Tovar, Marcela Garzon-Tituana, Iratxe Uranga-Murillo, Maykel A. Arias, Eva M. Galvez, Julian Pardo
Summary: In this article, the available experimental evidence on how NK cells kill target cells is reviewed and discussed, with a special focus on the different cell death modalities that have been found to be activated during NK cell-mediated cytotoxicity, including apoptosis and more inflammatory pathways like necroptosis and pyroptosis. Based on this new evidence, NK cell-induced cell death is proposed as a new regulatory mechanism linking innate immune response with the activation of tumor adaptive T cell responses.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Chanwoo Kim, Hanbin Lee, Juhee Jeong, Keehoon Jung, Buhm Han
Summary: The standard analysis pipeline for single-cell RNA-seq data can be limited by imperfect clustering results. To address this, a new method called MarcoPolo is proposed, which identifies differentially expressed genes independently of prior clustering. MarcoPolo evaluates gene distributions, expression patterns, and cell proximity in a low-dimensional space to sort out informative genes. Experimental results show that MarcoPolo recovers marker genes better than competing methods and can distinguish cell types that are not distinguishable by standard clustering.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Multidisciplinary Sciences
Patrick A. Sandoz, Kyra Kuhnigk, Edina K. Szabo, Sarah Thunberg, Elina Erikson, Niklas Sandstroem, Quentin Verron, Andreas Brech, Carsten Watzl, Arnika K. Wagner, Evren Alici, Karl-Johan Malmberg, Michael Uhlin, Bjoern Oenfelt
Summary: Gamma delta T cells play a vital role in protecting against infections and tumors throughout life. Their cytotoxic functions are regulated by the availability of lytic molecules granzyme B and perforin. Live single-cell imaging provides new insights into the cytotoxic arsenal and functions of gamma delta T cells, which may aid in the development of more effective immunotherapies.
NATURE COMMUNICATIONS
(2023)
Article
Immunology
Lucia Martinez Cuesta, Andrea Verna, Sandra Perez
Summary: This study aimed to analyze the mRNA expression levels of PFN and GZMs A, B, K, H, and M in the neural tissues of calves infected with BoAHV-1 or BoAHV-5, and observe their changes in different stages of the infection cycle. The results showed that PFN and GZM K were upregulated during the acute infection stage of both BoAHV-1 and BoAHV-5. In the latency stage of BoAHV-5, significant upregulation of PFN, GZM K, and GZM H was detected. PFN and GZM A, K, and H expression was also upregulated during the reactivation stage of BoAHV-5. Therefore, the distinct expression patterns of PFN and GZMs may contribute to the neuropathogenesis differences between BoAHV-1 and BoAHV-5.
VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY
(2023)
Article
Immunology
Maria Andrea Hernandez-Castaneda, Marilyne Lavergne, Pierina Casanova, Bryan Nydegger, Carla Merten, Bibin Yesodha Subramanian, Patricia Matthey, Nils Lannes, Pierre-Yves Mantel, Michael Walch
Summary: Malaria is a serious health problem in developing countries, and Plasmodium parasites deplete cholesterol in red blood cells making their membranes vulnerable, but it is the exposure of phosphatidylserine that triggers resistance to eukaryotic pore-forming proteins in parasitized cells.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Cell Biology
Shoji F. Kenji, Keerthi Kurma, Brigitte Collet, Christelle Oblet, Laure Debure, Carmelo Di Primo, Laetitia Minder, Franck Verite, Yannic Danger, Mickael Jean, Aubin Penna, Nicolas Levoin, Patrick Legembre
Summary: CD95 is a death receptor that promotes oncogenesis through various molecular mechanisms. This receptor can undergo cleavage and modification in both cancer cells and normal cells, with the modified N-terminal region potentially regulating receptor aggregation and death signaling.
CELL DEATH & DISEASE
(2022)