Article
Biochemistry & Molecular Biology
Amanda Kristiansson, Jesper Bergwik, Abdul Ghani Alattar, Johan Flygare, Magnus Gram, Stefan R. Hansson, Martin L. Olsson, Jill R. Storry, Maria Allhorn, Bo Akerstrom
Summary: A1M plays a protective role in reducing hemolysis and maintaining the stability of red blood cells, minimizing cell damage caused by hemolysis. Experimental evidence shows that A1M can protect both human and murine red blood cells from hemolysis.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Jesper Bergwik, Amanda Kristiansson, Charlotte Welinder, Olga Goransson, Stefan R. Hansson, Magnus Gram, Lena Erlandsson, Bo Akerstrom
Summary: Alpha(1)-microglobulin (A1M) is a ubiquitous protein with reductase and radical- and heme-binding properties, mainly expressed in the liver and encoded by the AMBP gene. Knockout mouse models lacking A1M expression but with preserved bikunin expression showed increased bikunin levels in the liver but decreased levels in plasma, as well as signs of increased ER stress and phenotypic changes such as increased body weight and lipid accumulation. The results suggest a role of A1M in regulating the intracellular redox environment, particularly in the liver.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Jesper Bergwik, Amanda Kristiansson, Jorgen Larsson, Simon Ekstrom, Bo Akerstrom, Maria Allhorn
Summary: A1M can bind to heparin and HS, and can be purified from human plasma using heparin affinity chromatography and size exclusion chromatography. The binding strength is inversely proportional to salt concentration and directly proportional to the sulfation degree of heparin and HS.
Article
Biochemistry & Molecular Biology
Sumiah A. Alghareeb, Jawaher Alsughayyir, Mohammad A. Alfhili
Summary: This study found that α-MG has hemolytic and eryptotic activities, which are mediated through Ca2+ signaling, Rac1 GTPase activity, and oxidative injury. Furthermore, α-MG accelerates cellular aging and selectively targets platelets and monocytes.
Article
Environmental Sciences
Ting Li, Zhou Li, Jianfei Fu, Chunlan Tang, Liya Liu, Jin Xu, Jinshun Zhao, Zhen Li
Summary: This study investigated the cytotoxicity of nickel nanoparticles on HTR-8/SVneo cells in vitro. The results showed that nickel nanoparticles treatment reduced cell viability, increased oxidative stress, and impaired mitochondrial respiratory capacity. Additionally, it induced cell cycle arrest and apoptosis.
ENVIRONMENTAL RESEARCH
(2022)
Article
Biochemistry & Molecular Biology
Tanja Jankovic-Karasoulos, Dale McAninch, Clare Dixon, Shalem Y. -L. Leemaqz, Maxime Francois, Wayne R. Leifert, Dylan McCullough, Carmela Ricciardelli, Claire T. Roberts, Tina Bianco-Miotto
Summary: The study revealed that high zinc intake can result in decreased proliferation and viability of trophoblast cells, as well as increased oxidative stress levels. Further research is needed to understand the mechanisms behind these effects.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2021)
Article
Agriculture, Dairy & Animal Science
Shuangbo Huang, Zifang Wu, Zihao Huang, Xiangyu Hao, Longmiao Zhang, Chengjun Hu, Jianfu Wei, Jinping Deng, Chengquan Tan
Summary: Oxidative stress in placenta is associated with adverse pregnancy outcomes in sow. Cysteamine (CS) supplementation can alleviate placental oxidative stress, enhance angiogenesis, and improve reproductive performance in sows. The underlying mechanism for these positive effects may involve the activation of Stat3 in porcine vascular endothelial cells (PVECs).
JOURNAL OF ANIMAL SCIENCE AND BIOTECHNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maria Jose Yanez, Andrea Leiva
Summary: The placenta plays a crucial role in cholesterol biosynthesis, metabolism, and exchange between the maternal and fetal compartments. The mechanisms of cholesterol uptake and intracellular traffic in placental cells are not fully understood. Proteins such as LDLR, SR-BI, ABCA1, and ABCG1 are involved in the transport of cholesterol. The role of proteins related to intracellular cholesterol traffic in placental cells during normal and pathological pregnancies is still largely unknown. Oxidative stress induced by ROS has been implicated in lysosomal and mitochondrial dysfunction in hypercholesterolemic pregnancies.
Article
Biochemistry & Molecular Biology
Alessandro Rolfo, Stefano Cosma, Anna Maria Nuzzo, Chiara Salio, Laura Moretti, Marco Sassoe-Pognetto, Andrea Roberto Carosso, Fulvio Borella, Juan Carlos Cutrin, Chiara Benedetto
Summary: This study observed oxidative stress and mitochondrial pathological changes in the placenta of pregnant women with SARS-CoV-2 infection during the third trimester, but the pregnant women maintained a good physiological state. This suggests that the placenta has a certain inhibitory effect on oxidative stress during SARS-CoV-2 infection.
Article
Biochemistry & Molecular Biology
Srividya Ganapathy, Elisa R. Farrell, Simran Vaghela, Lucy Joshee, Earl G. Ford, Olga Uchakina, Robert J. McKallip, Jennifer L. Barkin, Christy C. Bridges
Summary: This study assessed the transport of MeHg into placental syncytiotrophoblasts and characterized the mechanisms by which it exerts toxic effects. MeHg compounds were found to be transported into cells primarily by sodium-independent amino acid carriers and organic anion transporters. MeHg altered mitochondrial function and viability, decreased mitophagy and autophagy, and increased oxidative stress. Exposure to higher concentrations of MeHg inhibited the ability of cells to protect against MeHg-induced injury.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Ewa Mlyczynska, Malgorzata Myszka, Patrycja Kurowska, Monika Dawid, Tomasz Milewicz, Marta Balajewicz-Nowak, Pawel Kowalczyk, Agnieszka Rak
Summary: The study demonstrated the antiapoptotic effect of apelin on trophoblast cells by regulating the expression of pro-survival and proapoptotic factors, as well as attenuating oxidative stress. This effect was mediated by the APJ receptor and specific signaling pathways like mitogen-activated protein kinase (ERK1/2/MAP3/1) and protein kinase B (AKT), suggesting the potential role of apelin in placental development.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Svetlana Trifunovic, Branka Sosic Jurjevic, Natasa Ristic, Natasa Nestorovic, Branko Filipovic, Ivana Stevanovic, Vesna Begovic-Kupresanin, Milica Manojlovic-Stojanoski
Summary: The placenta plays an important role in providing a suitable environment for fetal growth. This study found that there are sex differences in the placenta's morphological features and antioxidant status after exposure to dexamethasone (Dx). Female placentas showed a greater decrease in volume and antioxidant enzyme activities, and an increase in lipid peroxides and nitric oxide concentration compared to male placentas.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Hamid-Reza Kohan-Ghadr, Brooke Armistead, Mikaela Berg, Sascha Drewlo
Summary: Irisin is a newly discovered exercise-mediated polypeptide hormone. Studies have shown that Irisin protects against cell death by reducing pro-apoptotic signaling cascades, inducing DNA repair pathways, and inhibiting Caspase 3 activity. Additionally, Irisin acts through the Akt signaling pathway to prevent apoptosis, increase levels of anti-apoptotic BCL2, and reduce ROS levels. These findings provide new insights into the role of Irisin in promoting cell survival and preventing apoptosis in the human placenta under pathological conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Nikola Sobocan, Marta Himelreich-Peric, Ana Katusic-Bojanac, Jure Krasic, Nino Sincic, Zeljka Majic, Gordana Juric-Lekic, Ljiljana Serman, Andreja Maric, Davor Jezek, Floriana Bulic-Jakus
Summary: The antioxidant PBN partially protects embryos from the negative effects of a DNA demethylating drug during pregnancy. This study investigated PBN's impact on the placenta and found that PBN exerted its prophylactic activity against the drug in the placenta through free radical scavenging.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Agriculture, Dairy & Animal Science
Valeria Pasciu, Francesca D. Sotgiu, Cristian Porcu, Fiammetta Berlinguer
Summary: Glycerol is commonly used as a feed supplement in ruminant nutrition, but it can lead to hemolysis at high concentrations. However, the antioxidant defenses of red blood cells can protect against oxidative stress caused by glycerol-induced osmotic stress.
Article
Biochemistry & Molecular Biology
Amanda Kristiansson, Jesper Bergwik, Abdul Ghani Alattar, Johan Flygare, Magnus Gram, Stefan R. Hansson, Martin L. Olsson, Jill R. Storry, Maria Allhorn, Bo Akerstrom
Summary: A1M plays a protective role in reducing hemolysis and maintaining the stability of red blood cells, minimizing cell damage caused by hemolysis. Experimental evidence shows that A1M can protect both human and murine red blood cells from hemolysis.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Article
Biochemistry & Molecular Biology
Jesper Bergwik, Amanda Kristiansson, Charlotte Welinder, Olga Goransson, Stefan R. Hansson, Magnus Gram, Lena Erlandsson, Bo Akerstrom
Summary: Alpha(1)-microglobulin (A1M) is a ubiquitous protein with reductase and radical- and heme-binding properties, mainly expressed in the liver and encoded by the AMBP gene. Knockout mouse models lacking A1M expression but with preserved bikunin expression showed increased bikunin levels in the liver but decreased levels in plasma, as well as signs of increased ER stress and phenotypic changes such as increased body weight and lipid accumulation. The results suggest a role of A1M in regulating the intracellular redox environment, particularly in the liver.
FREE RADICAL BIOLOGY AND MEDICINE
(2021)
Correction
Multidisciplinary Sciences
Magnus Gram, Ulrik Dolberg Anderson, Maria E. Johansson, Anneli Edstrom-Hagerwall, Irene Larsson, Maya Jalmby, Stefan R. Hansson, Bo Akerstrom
Correction
Physiology
David Ley, Olga Romantsik, Suvi Vallius, Kristbjorg Sveinsdottir, Snjolaug Sveinsdottir, Alex A. Agyemang, Maria Baumgarten, Matthias Morgelin, Nataliya Lutay, Matteo Bruschettini, Bo Holmqvist, Magnus Gram
FRONTIERS IN PHYSIOLOGY
(2020)
Article
Immunology
Alex Adusei Agyemang, Suvi Vallius Kvist, Nathan Brinkman, Thomas Gentinetta, Miriam Illa, Niklas Ortenlof, Bo Holmqvist, David Ley, Magnus Gram
Summary: Cell-free hemoglobin plays a central role in the pathophysiology of GM-IVH in preterm infants, and early administration of the hemoglobin scavenger Hp may provide therapeutic benefits.
JOURNAL OF NEUROINFLAMMATION
(2021)
Review
Physiology
Jesper Bergwik, Amanda Kristiansson, Maria Allhorn, Magnus Gram, Bo Akerstrom
Summary: A1M is an antioxidant and tissue cleaning protein with reductase, heme- and radical-binding activities. It has been shown to have protective effects in oxidative stress-related medical conditions and has a unique gene coding structure.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Amanda Kristiansson, Anders Orbom, Jonas Ahlstedt, Helena Karlsson, Wahed Zedan, Magnus Gram, Bo Akerstrom, Sven-Erik Strand, Mohamed Altai, Joanna Strand, Oskar Vilhelmsson Timmermand
Summary: The study investigated the combination therapy of human radical scavenger and radioligand therapy in terms of therapeutic efficacy and kidney toxicity, showing that the combination treatment has a positive effect on kidney function without affecting the efficacy of anti-PSMA radioligand therapy.
Article
Biochemistry & Molecular Biology
Selvaraju Kanagarajan, Magnus L. R. Carlsson, Sandeep Chakane, Karin Kettisen, Emanuel Smeds, Ranjeet Kumar, Niklas Ortenlof, Magnus Gram, Bo Akerstrom, Leif Bulow, Li-Hua Zhu
Summary: This study successfully produced recombinantly fused endotoxin-free HbF in Nicotiana benthamiana through Agrobacterium tumefaciens-mediated transient expression, demonstrating its high potential for use as blood substitutes. The recombinant protein showed structural and functional properties similar to native HbF, confirmed by various analyses including SDS-PAGE, Western blot, and mass spectrometry.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Biochemistry & Molecular Biology
David Petho, Tamas Gall, Zoltan Hendrik, Annamaria Nagy, Livia Beke, Attila Peter Gergely, Gabor Mehes, Csaba Toth, Magnus Gram, Bo Akerstrom, Gyorgy Balla, Jozsef Balla
Summary: This study investigates the origin and role of A1M in human atherosclerotic lesions, showing its presence in plaques and induction by heme and FerrylHb in resident cells. A1M inhibited Hb oxidation and heme-driven lipid peroxidation in ruptured lesions, suggesting a potential protective function against atherosclerosis progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Hematology
Sara E. Sjogren, Jun Chen, Alexander Mattebo, Abdul G. Alattar, Helena Karlsson, Kavitha Siva, Shamit Soneji, Ulf Tedgard, Jane-Jane Chen, Magnus Gram, Johan Flygare
Summary: Diamond-Blackfan anemia is a rare genetic disorder characterized by a scarcity of erythroid precursors in the bone marrow, with most patients carrying mutations in ribosomal proteins. Targeting elevated heme levels may be a promising new treatment strategy for DBA, as it improves disease symptoms and provides insights for future therapies.
EXPERIMENTAL HEMATOLOGY
(2022)
Article
Medicine, General & Internal
Asa Jungner, Suvi Vallius, Magnus Gram, David Ley
Summary: During neonatal cardiopulmonary bypass, there is an increase in plasma concentrations of cell-free hemoglobin and a decrease in scavenger protein supplies. The concentration of cell-free hemoglobin in blood prime solution is a major predictor of cell-free hemoglobin exposure.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Robert Greite, Li Wang, Lukas Gohlke, Sebastian Schott, Kirill Kreimann, Julian Doricic, Andreas Leffler, Igor Tudorache, Jawad Salman, Ruslan Natanov, Fabio Ius, Christine Fegbeutel, Axel Haverich, Ralf Lichtinghagen, Rongjun Chen, Song Rong, Hermann Haller, Vijith Vijayan, Magnus Gram, Irina Scheffner, Faikah Gueler, Wilfried Gwinner, Stephan Immenschuh
Summary: This study investigated the levels of CFH and CFH-binding hemoglobin in LuTx patients and found that CFH may be associated with postoperative AKI. Moreover, experimental studies on a mouse model of mild renal ischemia reperfusion injury confirmed the role of CFH in promoting AKI. Targeting CFH may be a therapeutic option to prevent AKI after LuTx.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Multidisciplinary Sciences
Nishte Rassol, Charlotte Andersson, Daniella Pettersson, Amin Al-Awar, Emman Shubbar, Aniko Kovacs, Bo Akerstrom, Magnus Gram, Khalil Helou, Eva Forssell-Aronsson
Summary: The aim of this study was to investigate the regulation of apoptosis-related genes in GOT1 tumors after administration of Lu-177-octreotate with or without A1M. The results showed that co-administration of A1M did not negatively affect the apoptotic response of Lu-177-octreotate in GOT1 tumors.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Abdul Ghani Alattar, Amanda Kristiansson, Helena Karlsson, Suvi Vallius, Jonas Ahlstedt, Eva Forssell-Aronsson, Bo Akerstrom, Sven-Erik Strand, Johan Flygare, Magnus Gram
Summary: Lu-177-DOTATATE PRRT is an effective therapy for neuroendocrine tumors, but it can cause bone marrow suppression and renal toxicity. A1M, a recombinant antioxidant, has been shown to protect the kidneys in preclinical models. In this study, intravenous administration of rA1M reduced albuminuria levels and oxidative stress-related protein FGF-21 in mice treated with Lu-177-DOTATATE. Dual injections of rA1M preserved bone marrow cellularity and peripheral blood reticulocytes. This suggests that rA1M, when administered with Lu-177-DOTATATE, may reduce hematopoietic and kidney toxicity.
Article
Developmental Biology
Magnus Gram, Claes Ekstrom, Bo Holmqvist, Galen Carey, Xiaoyang Wang, Suvi Vallius, William Hellstrom, Niklas Ortenlof, Alex Adusei Agyemang, Lois E. H. Smith, Ann Hellstrom, Alexandra Mangili, Norman Barton, David Ley
Summary: This study investigates the impact of changes in IGF-1 levels after preterm birth and the effects of recombinant IGF-1/IGFBP-3 on preventing intraventricular hemorrhage in preterm infants. The results show that IGF-1 can affect cerebrovascular maturation and may be beneficial for preventing preterm IVH.
DEVELOPMENTAL NEUROSCIENCE
(2021)