Article
Agronomy
Qingxi Yang, Qian Zhou, Xin Zhou, Huixin Fang, Yingbo Zhao, Baodong Wei, Shujuan Ji
Summary: Postharvest yellowing is the main manifestation of rapid deterioration in broccoli, leading to a significant reduction in glucosinolates content. The reduction in glucoraphanin and sulforaphane content is mainly attributed to the continuous down-regulation of related genes.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2022)
Article
Plant Sciences
Zhipeng Li, Jinyu Bao, Xu Lu, Yaqi Lu, Peng Tian, Xiaoling Zhang, Jie Yang, Zhihui Pu, Sheng Li
Summary: Exogenous melatonin treatment increased the content of glucoraphanin and sulforaphane in broccoli roots, with transcriptome sequencing revealing a strong response in the plant hormone signaling transduction pathway. Enzyme-linked immunosorbent assay confirmed the promotion of four phytohormones by the melatonin treatment. This study provides a theoretical basis for understanding the complex molecular mechanism by which melatonin regulates secondary metabolites through mediating plant hormone signal transduction.
JOURNAL OF PLANT GROWTH REGULATION
(2023)
Review
Chemistry, Applied
Jing Sun, Yunfan Wang, Xinyi Pang, Shuhua Tian, Qiaobin Hu, Xiangfei Li, Jie Liu, Jing Wang, Yingjian Lu
Summary: Various cooking methods have different effects on the retention of GLR and the formation of SLR, with freezing helping to avoid GLR loss and short-time microwaving, short-time steaming, and fermentation promoting the biotransformation from GLR to SLR; Boiling and blanching cause the largest losses of GLR and SLR, while freezing significantly protects their losses; Stir-frying varies the levels of GLR and SLR in different cooking conditions.
Article
Horticulture
Jung Su Jo, Shiva Ram Bhandari, Gwan Ho Kang, Yu Kyeong Shin, Jun Gu Lee
Summary: This study investigated the GSL profile, hydrolysate and nitrile contents, and nitrile formation rate of three commercial cultivars and eleven inbred broccoli lines. The results showed that glucobrassicin and glucoraphanin were the most dominant GSLs in all lines. Based on GSL content, five lines were selected as potential highly functional broccoli lines. Three lines exhibited relatively higher total hydrolysates. Three inbred lines with low nitrile formation rate and high isothiocyanate formation were selected as functional broccoli breeding lines. Therefore, hydrolysate and GSL content are deemed more reliable for selecting and developing highly functional varieties.
SCIENTIA HORTICULTURAE
(2022)
Article
Biochemistry & Molecular Biology
Sajad Shokri, Hema Jegasothy, Mary Ann Augustin, Netsanet Shiferaw Terefe
Summary: The study showed that thermosonication can enhance the yield of sulforaphane in broccoli, despite a decrease in myrosinase activity. The highest yield was achieved under the treatment of thermosonication at 60 degrees Celsius for 7 minutes, which also increased the residual level of sulforaphane, indicating the potential for converting broccoli biomass into high sulforaphane ingredients.
Article
Nutrition & Dietetics
Tracey L. Livingstone, Shikha Saha, Federico Bernuzzi, George M. Savva, Perla Troncoso-Rey, Maria H. Traka, Robert D. Mills, Richard Y. Ball, Richard F. Mithen
Summary: Consuming supplements containing glucoraphanin and alliin can increase the levels of sulforaphane in prostate tissue, but does not significantly affect the concentration of alliin and its metabolites in the prostate.
Article
Acoustics
Beini Liu, Yang Tao, Sivakumar Manickam, Dandan Li, Yongbin Han, Ying Yu, Dongfeng Liu
Summary: In this study, the authors investigated the drying of broccoli florets using exogenous pretreatment and contact ultrasound-assisted drying. They analyzed various components and factors involved in the metabolism pathway to understand the effects of the dehydration methods on the conversion of glucoraphanin to sulforaphane. The results showed that the sequential exogenous pretreatment and ultrasound drying shortened the drying time and both melatonin and vitamin C pretreatment protected the sulforaphane content and myrosinase activity. This study enriches the biochemistry knowledge about the transformation of glucoraphanin to sulforaphane and suggests that the combined pretreatment and ultrasound drying can preserve bioactive sulforaphane in dehydrated broccoli.
ULTRASONICS SONOCHEMISTRY
(2022)
Article
Agronomy
Lorena Martinez-Zamora, Noelia Castillejo, Francisco Artes-Hernandez
Summary: This study found that UV lighting can reduce microbial load and increase phenolic content and antioxidant capacity in sprouts. UV treatments also enhance glucosinolate and isothiocyanate content in radish and broccoli sprouts, which contributes to the prevention of chronic inflammatory diseases.
POSTHARVEST BIOLOGY AND TECHNOLOGY
(2021)
Article
Chemistry, Applied
Lili Wang, Hamed I. Hamouda, Yueyang Dong, Hong Jiang, Yongyi Quan, Yimiao Chen, Yan Liu, Jiaqi Wang, Mohamed A. Balah, Xiangzhao Mao
Summary: A yeast strain called Yarrowia lipolytica 20-8 with high glucoraphanin-hydrolyzing activity was found to be effective in the preparation of sulforaphane. The reaction efficiency reached 99.86% with a production of 10.32 mg sulforaphane from 1 g broccoli seed in just 15 minutes using a yeast whole-cell catalyst. The catalyst could also be reused multiple times with a retention rate of 87.56-92.53% of the initial sulforaphene yield.
Article
Nutrition & Dietetics
Salah Abukhabta, Sameer Khalil Ghawi, Kimon Andreas Karatzas, Dimitris Charalampopoulos, Gordon J. Mcdougall, J. Will Allwood, Susan R. Verrall, Siobhan Lavery, Cheryl Latimer, L. Kirsty Pourshahidi, Roger Lawther, Gloria O'Connor, Ian Rowland, Chris I. R. Gill
Summary: Studies suggest that Sulforaphane-rich broccoli extracts have potent antimicrobial effects in vitro, and the consumption of Sulforaphane-enriched broccoli soup may inhibit bacterial growth in the stomach and upper small intestine, but not in the terminal ileum or the colon.
EUROPEAN JOURNAL OF NUTRITION
(2021)
Review
Oncology
Dana Elkhalifa, Nour Al-Ziftawi, Ahmed Awaisu, Feras Alali, Ashraf Khalil
Summary: This systematic review aimed at assessing the therapeutic potential of sulforaphane (SFN) in diverse cancer types. The review identified 8 human-based RCTs investigating the efficacy and safety of SFN in prostate cancer, breast cancer, pancreatic cancer, and melanoma. SFN treatment led to statistically significant alterations in several vital genes and histological biomarkers. However, further robust clinical investigations are needed to conclusively ascertain the chemotherapeutic potential of SFN.
FRONTIERS IN ONCOLOGY
(2023)
Article
Nutrition & Dietetics
Martin Magner, Katerina Thorova, Veronika Zupova, Milan Houska, Ivana Svandova, Pavla Novotna, Jan Triska, Nadezda Vrchotova, Ivo Soural, Ladislav Jilek
Summary: This study examined the effects of sulforaphane on autistic behavior in children aged 3-7 years with autism spectrum disorder. However, the results showed no significant improvement in the behavioral outcome measures after 36 weeks of treatment with sulforaphane.
Article
Immunology
Omar Guadarrama-Enriquez, Gabriel Fernando Moreno-Perez, Maria Eva Gonzalez-Trujano, Guadalupe Esther Angeles-Lopez, Rosa Ventura-Martinez, Irene Diaz-Reval, Agustina Cano-Martinez, Francisco Pellicer, Nieves Baenas, Diego A. Moreno, Cristina Garcia-Viguera
Summary: This study investigates the analgesic and anti-inflammatory activities of an aqueous extract of Brassica oleracea var. italica sprouts (AEBS) and its active metabolite sulforaphane (SFN). The results demonstrate the potential of AEBS and SFN as natural products for the treatment of pain and inflammation.
INFLAMMOPHARMACOLOGY
(2023)
Article
Nutrition & Dietetics
John A. Bouranis, Laura M. Beaver, Jaewoo Choi, Carmen P. Wong, Duo Jiang, Thomas J. Sharpton, Jan F. Stevens, Emily Ho
Summary: Research indicates that the production of isothiocyanates may be influenced by the composition of the gut microbiome, potentially impacting the efficacy of interventions with cruciferous vegetables. In humans, there is significant inter-individual variability in SFN-NIT excretion following consumption of broccoli sprouts.
Article
Biotechnology & Applied Microbiology
Sajad Shokri, Hema Jegasothy, Mya Myintzu Hliang, Mary Ann Augustin, Netsanet Shiferaw Terefe
Summary: The effects of thermosonication and thermal treatment on the bioactive components of fermented broccoli puree were compared in this study. Both treatments increased the rate of acidification and the yield of sulforaphane in the broccoli puree. The highest yield of sulforaphane was observed in the thermosonicated samples, indicating increased extractability and accessibility of glucoraphanin.
FERMENTATION-BASEL
(2022)