Review
Biochemistry & Molecular Biology
Martim Dias Gomes, Sandra Iden
Summary: Eukaryotic development relies on dynamic cell shape changes and segregation of fate determinants for coordinated compartmentalization. Recent studies have revealed an interplay of polarity proteins, adhesion molecules, and mechanochemical pathways in tissue organization. The cell polarity field has evolved into a multidisciplinary ground, with significant advances in biophysics, genome editing, high-resolution imaging, and mathematical modeling.
Article
Biology
Karthik Chandiran, Jenny E. Suarez-Ramirez, Yinghong Hu, Evan R. Jellison, Zeynep Ugur, Jun-Siong Low, Bryan McDonald, Susan M. Kaech, Linda S. Cauley
Summary: Transforming growth factor beta (TGF beta) uses SMAD-dependent signaling to regulate transcriptional programming of pathogen-specific cytotoxic T lymphocytes (CTLs), altering the expression levels of multiple homing receptors and transcription factors. These molecules play a crucial role in determining the localization of pathogen-specific CTLs in circulation or peripheral tissues.
Article
Reproductive Biology
Weimin Liu, Jing Chen, Chen Yang, Kai-Fai Lee, Yin-Lau Lee, Philip Chi-Ngong Chiu, Yihui Zhang, Yong-Gong Duan, Kui Liu, William Shu-Biu Yeung
Summary: During mouse embryo development, the expression of let-7 microRNA varies at different stages, playing an important regulatory role in embryonic development and blastocyst formation. High expression of let-7 at the 2-cell stage favors the formation of the inner cell mass, while low expression at the 4-8 cell stages enhances blastocyst formation. Tead4 mediates the action of let-7 on the determination of inner cell mass cell fate.
BIOLOGY OF REPRODUCTION
(2022)
Article
Oncology
Theresa Eifert, Chen-Jen Hsu, Alicia L. Becker, Sarah Graessle, Arik Horne, Franziska Bemmann, Qirui Zhang, Michael Heuser, Valeri Vasioukhin, Sebastian Scholl, Andreas Hochhaus, Florian Siegerist, Nicole Endlich, Lars Bullinger, Steven W. Lane, Simon Haas, Tina M. Schnoeder, Florian H. Heidel
Summary: The Scribble complex proteins play a crucial role in hematopoietic cell fate decisions and self-renewal capacity. Through genetic screening, LLGL1 has been identified as a specific dependency and putative target in AML, with its inactivation leading to loss of stemness-associated gene-expression and induction of a specific phenotype in leukemia stem cells. These findings highlight the importance of LLGL1 and its cell-type specific functions in AML.
Editorial Material
Immunology
Tao Wu, Li Wu
Summary: In this study, Tian and colleagues successfully identified Bcor as a negative regulator for dendritic cell differentiation using innovative experimental methods.
Article
Engineering, Biomedical
Yiming Wang, Gangqing Hu, Ryan C. Hill, Monika Dzieciatkowska, Kirk C. Hansen, Xiao-Bing Zhang, Zuoqin Yan, Ming Pei
Summary: This study demonstrates that expansion on decellularized extracellular matrix (dECM) can rejuvenate high-passage infrapatellar fat pad stem cells (IPFSCs) by reversing cellular senescence. SV40LT transduced IPFSCs showed increased proliferation and adipogenic potential but decreased chondrogenic potential. Matrix components like basement membrane proteins were identified as key factors in matrix-mediated rejuvenation of IPFSCs.
Review
Cell Biology
Mengchen Fan, Ying Shi, Jumei Zhao, Ling Li
Summary: Cancer stem cells (CSCs) are responsible for tumor recurrence and metastasis, and understanding the mechanisms behind CSC stemness maintenance and cell fate determination is crucial for cancer therapy. Recent studies have shown that mitochondrial metabolism plays an active role in determining CSC fate by affecting stemness gene expression. In this review, we discuss the influence of mitochondria on CSC potential from the perspective of mito-nuclear communication, focusing on metabolism, dynamics, mitochondrial homeostasis, and reactive oxygen species (ROS).
CELL COMMUNICATION AND SIGNALING
(2023)
Article
Developmental Biology
Chenchao Yan, Yajing Meng, Jie Yang, Jian Chen, Wei Jiang
Summary: This study investigates the translational regulation during early human neural differentiation by performing ribosome sequencing and RNA sequencing on human embryonic stem cells (ESCs) and neural progenitor cells (NPCs). The findings reveal the importance of transcriptional and post-transcriptional regulation in neural fate determination, as well as the role of untranslated region (UTR) sequence characteristics in translation efficiency.
Article
Chemistry, Multidisciplinary
Jiong-hua Huang, Chuang-jia Huang, Li-na Yu, Xiao-ling Guan, Shang-wen Liang, Jian-hong Li, Lu Liang, Min-yan Wei, Ling-min Zhang
Summary: Atherosclerosis is a major cause of death and disability in cardiovascular disease. The polarization of M2 macrophages plays a crucial role in the development of atherosclerosis plaque. A recent study found that TRIM24 inhibition can promote M2 macrophage polarization. By loading TRIM24 onto nanoparticles and coating them with M2 macrophage membrane, it can be used for the treatment of atherosclerosis.
ACTA PHARMACOLOGICA SINICA
(2023)
Article
Cell Biology
Oz Pomp, Hui Yi Grace Lim, Robin M. Skory, Adam A. Moverley, Piotr Tetlak, Stephanie Bissiere, Nicolas Plachta
Summary: This study reveals that heterogeneities in cell polarity in early mouse embryos trigger the assembly of a highly asymmetric spindle organization, determining the fate of inner cell mass versus trophectoderm.
NATURE CELL BIOLOGY
(2022)
Review
Cell Biology
Iris J. A. Peters, Emma de Pater, Wei Zhang
Summary: The correct maintenance and differentiation of hematopoietic stem cells (HSC) in bone marrow, regulated by GATA2, is crucial for the functioning of the human blood system. GATA2 deficiency syndrome, caused by mutations in GATA2, leads to various defects and diseases, yet the underlying mechanisms remain to be fully understood. This review summarizes recent advances in understanding the role of GATA2 in determining the fate of hematopoietic cells and discusses the challenges of modeling GATA2 deficiency syndrome.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Evan Ja-Yang Wang, I-Hsuan Chen, Brian Yu-Ting Kuo, Chia-Cheng Yu, Ming-Tsung Lai, Jen-Tai Lin, Leo Yen-Ting Lin, Chih-Mei Chen, Tritium Hwang, Jim Jinn-Chyuan Sheu
Summary: Cytoskeleton proteins provide mechanical support for cell development and tissue homeostasis. Mutated genes associated with cytoskeletal proteins have been found in cancer, some of which have clinical implications. Cytoskeleton-associated proteins also play important signaling roles in cancer development. Mutations in cytoskeleton components can change the physical/mechanical properties of cells and determine cell fate during cancer development.
Review
Pharmacology & Pharmacy
Mengmeng Yuan, Lucy Zhao, Yuhong Li, Xiumei Gao, Boli Zhang, Deqin Zhang, Yue Li
Summary: Capsaicin, the main active component of chili peppers, plays a crucial role in the proliferation and fate specification of stem cells by modulating various signaling pathways such as PPAR gamma and Notch signaling. Understanding the complex and multifactorial processes mediated by capsaicin can provide insights for its clinical applications in stem cell therapy for treating diseases.
PHARMACOLOGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Muhammad Abid Sheikh, Bright Starling Emerald, Suraiya Anjum Ansari
Summary: Embryonic and adult stem cells have the ability of self-renewal and lineage-specific differentiation, which is regulated by metabolic pathways and epigenetic modifications such as O-GlcNAcylation. Nutrient availability inside the cells can affect GlcNAc levels, thereby influencing stem cell fate decisions.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Cell Biology
Hongxin Zhong, Jie Zhang, Yuli Lu, Gongcheng Hu, Guangjin Pan, Hongjie Yao
Summary: The nucleosome is the basic subunit of chromatin, formed by histone octamers and genomic DNA. By folding and compressing, these structures form the 30-nm chromatin fiber, which is further organized within the nucleus in a hierarchical manner, known as the 3D genome. Understanding chromatin structure and regulatory changes in chromatin is crucial for studying cellular architecture, function, cell fate determination, regeneration, and diseases.
CELL PROLIFERATION
(2023)
Article
Gastroenterology & Hepatology
Parambir S. Dulai, Siddharth Singh, Niels Vande Casteele, Joseph Meserve, Adam Winters, Shreya Chablaney, Satimai Aniwan, Preeti Shashi, Gursimran Kochhar, Aaron Weiss, Jenna L. Koliani-Pace, Youran Gao, Brigid S. Boland, John T. Chang, David Faleck, Robert Hirten, Ryan Ungaro, Dana Lukin, Keith Sultan, David Hudesman, Shannon Chang, Matthew Bohm, Sashidhar Varma, Monika Fischer, Eugenia Shmidt, Arun Swaminath, Nitin Gupta, Maria Rosario, Vipul Jairath, Leonardo Guizzetti, Brian G. Feagan, Corey A. Siegel, Bo Shen, Sunanda Kane, Edward V. Loftus, William J. Sandborn, Bruce E. Sands, Jean-Frederic Colombel, Karen Lasch, Charlie Cao
CLINICAL GASTROENTEROLOGY AND HEPATOLOGY
(2020)
Article
Multidisciplinary Sciences
Robert A. Quinn, Alexey Melnik, Alison Vrbanac, Ting Fu, Kathryn A. Patras, Mitchell P. Christy, Zsolt Bodai, Pedro Belda-Ferre, Anupriya Tripathi, Lawton K. Chung, Michael Downes, Ryan D. Welch, Melissa Quinn, Greg Humphrey, Morgan Panitchpakdi, Kelly C. Weldon, Alexander Aksenov, Ricardo da Silva, Julian Avila-Pacheco, Clary Clish, Sena Bae, Himel Mallick, Eric A. Franzosa, Jason Lloyd-Price, Robert Bussell, Taren Thron, Andrew T. Nelson, Mingxun Wang, Eric Leszczynski, Fernando Vargas, Julia M. Gauglitz, Michael J. Meehan, Emily Gentry, Timothy D. Arthur, Alexis C. Komor, Orit Poulsen, Brigid S. Boland, John T. Chang, William J. Sandborn, Meerana Lim, Neha Garg, Julie C. Lumeng, Ramnik J. Xavier, Barbara Kazmierczak, Ruchi Jain, Marie Egan, Kyung E. Rhee, David Ferguson, Manuela Raffatellu, Hera Vlamakis, Gabriel G. Haddad, Dionicio Siegel, Curtis Huttenhower, Sarkis K. Mazmanian, Ronald M. Evans, Victor Nizet, Rob Knight, Pieter C. Dorrestein
Article
Immunology
J. Justin Milner, Clara Toma, Zhaoren He, Nadia S. Kurd, Quynh P. Nguyen, Bryan McDonald, Lauren Quezada, Christella E. Widjaja, Deborah A. Witherden, John T. Crowl, Laura A. Shaw, Gene W. Yeo, John T. Chang, Kyla D. Omilusik, Ananda W. Goldrath
Article
Immunology
Brigid S. Boland, Zhaoren He, Matthew S. Tsai, Jocelyn G. Olvera, Kyla D. Omilusik, Han G. Duong, Eleanor S. Kim, Abigail E. Limary, Wenhao Jin, J. Justin Milner, Bingfei Yu, Shefali A. Patel, Tiani L. Louis, Tiffani Tysl, Nadia S. Kurd, Alexandra Bortnick, Lauren K. Quezada, Jad N. Kanbar, Ara Miralles, Danny Huylebroeck, Mark A. Valasek, Parambir S. Dulai, Siddharth Singh, Li-Fan Lu, Jack D. Bui, Cornelis Murre, William J. Sandborn, Ananda W. Goldrath, Gene W. Yeo, John T. Chang
SCIENCE IMMUNOLOGY
(2020)
Review
Medicine, General & Internal
John T. Chang
NEW ENGLAND JOURNAL OF MEDICINE
(2020)
Article
Multidisciplinary Sciences
Debashis Sahoo, Lee Swanson, Ibrahim M. Sayed, Gajanan D. Katkar, Stella-Rita Ibeawuchi, Yash Mittal, Rama F. Pranadinata, Courtney Tindle, Mackenzie Fuller, Dominik L. Stec, John T. Chang, William J. Sandborn, Soumita Das, Pradipta Ghosh
Summary: Modeling human diseases as networks and utilizing machine learning successfully predicted outcomes and identified a novel therapy for inflammatory bowel disease, improving the integrity of the gut epithelial barrier.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Shengyun Ma, Shefali A. Patel, Yohei Abe, Nicholas Chen, Parth R. Patel, Benjamin S. Cho, Nazia Abbasi, Suling Zeng, Bernd Schnabl, John T. Chang, Wendy Jia Men Huang
Summary: RAR-related orphan receptor-gamma (ROR gamma t) is a critical transcription factor for thymic T cell development, lymphoid tissue organogenesis, and immune cell differentiation. Serine 182 phosphorylation on ROR gamma t serves as a key regulator, simultaneously restricting Th17 hyperactivation and promoting anti-inflammatory cytokine IL-10 production.
Article
Gastroenterology & Hepatology
Brigid S. Boland, Benjamin Goodwin, Zeli Zhang, Nathaniel Bloom, Yu Kato, Jennifer Neill, Helen Le, Tiffani Tysl, Angelina E. Collins, Parambir S. Dulai, Siddharth Singh, Nghia H. Nguyen, Alba Grifoni, Alessandro Sette, Daniela Weiskopf, John T. Chang, Jennifer M. Dan
Summary: This study assessed the differences in SARS-CoV-2 vaccine immunogenicity between patients with IBD treated with different immune-modulating medications and normal healthy donors. The results showed reduced humoral immunity in patients with IBD on infliximab, but no difference in humoral and cell-mediated immunity in patients with IBD on infliximab with a thiopurine or vedolizumab compared with normal healthy donors.
CLINICAL AND TRANSLATIONAL GASTROENTEROLOGY
(2022)
Article
Immunology
John T. Crowl, Maximilian Heeg, Amir Ferry, J. Justin Milner, Kyla D. Omilusik, Clara Toma, Zhaoren He, John T. Chang, Ananda W. Goldrath
Summary: This study investigates the diversity of gene expression and genome accessibility in mouse CD8(+) T-RM cells in different tissues, and identifies molecules critical for the generation of CD8(+) T-RM cells in response to acute viral infection. The research reveals both common and tissue-specific transcriptional and epigenetic signatures in T-RM cells from different organs. T-RM cells in the intestine and salivary glands express TGF-beta-induced genes and are maintained by ongoing TGF-beta signaling, while those in the fat, kidney, and liver do not. Furthermore, the study identifies the transcriptional repressor Hic1 as a critical regulator of T-RM cell differentiation in the small intestine, and shows that Hic1 overexpression enhances T-RM cell differentiation and protection from infection.
Article
Immunology
Yun Hsuan Lin, Han G. Duong, Abigail E. Limary, Eleanor S. Kim, Paul Hsu, Shefali A. Patel, William H. Wong, Cynthia S. Indralingam, Yi Chia Liu, Priscilla Yao, Natalie R. Chiang, Taylor R. Anderson, Jocelyn G. Olvera, Amir Ferry, Kennidy K. Takehara, Wenhao Jin, Matthew S. Tsai, Gene W. Yeo, Ananda W. Goldrath, John T. Chang
Summary: Tissue-resident memory CD8+ T (TRM) cells are a subset of memory T cells that are important for controlling infection. TRM cells in the small intestine and colon exhibit heterogeneity in terms of cytokine and granzyme expression, transcriptional and epigenetic regulation, and functional characteristics. The transcription factor Eomes plays a context-specific role in supporting the maintenance of established TRM cells in the small intestine but not in the colon.
Article
Biochemistry & Molecular Biology
Lauren K. Quezada, Wenhao Jin, Yi Chia Liu, Eleanor S. Kim, Zhaoren He, Cynthia S. Indralingam, Tiffani Tysl, Lara Labarta-Bajo, Ellen J. Wehrens, Yeara Jo, Katelynn R. Kazane, Christopher Hattori, Elina I. Zuniga, Gene W. Yeo, John T. Chang
Summary: During microbial infection, CD8(+) T cells can differentiate into effector cells or memory cells to provide host defense. However, in the context of chronic infections and cancer, CD8(+) T cells can become exhausted and dysfunctional. The factors regulating the early differentiation of exhausted CD8(+) T cells remain incompletely understood.
Article
Multidisciplinary Sciences
Aaron T. Mayer, Derek R. Holman, Anav Sood, Utkarsh Tandon, Salil S. Bhate, Sunil Bodapati, Graham L. Barlow, Jeff Chang, Sarah Black, Erica C. Crenshaw, Alexander N. Koron, Sarah E. Streett, Sanjiv S. Gambhir, William J. Sandborn, Brigid S. Boland, Trevor Hastie, Robert Tibshirani, John T. Chang, Garry P. Nolan, Christian M. Schuerch, Stephan Rogalla
Summary: This study uses CODEX technology to create a tissue atlas of inflammation in UC patients and healthy individuals. The analysis reveals the association between cellular functional states and cellular neighborhoods, as well as the presence of resistant niches in UC patients with TNFi treatment. Additionally, the study explores the use of CNNs in predicting patient clinical variables and provides guidelines for reporting predictions in similar datasets.
Article
Gastroenterology & Hepatology
Carlos G. Gonzalez, Robert H. Mills, Melissa C. Kordahi, Marvic Carrillo-Terrazas, Henry Secaira-Morocho, Christella E. Widjaja, Matthew S. Tsai, Yash Mittal, Brian A. Yee, Fernando Vargas, Kelly Weldon, Julia M. Gauglitz, Clara Delaroque, Consuelo Sauceda, Leigh-Ana Rossitto, Gail Ackermann, Gregory Humphrey, Austin D. Swafford, Corey A. Siegel, Jay C. Buckey, Laura E. Raffals, Charlotte Sadler, Peter Lindholm, Kathleen M. Fisch, Mark Valaseck, Arief Suriawinata, Gene W. Yeo, Pradipta Ghosh, John T. Chang, Hiutung Chu, Pieter Dorrestein, Qiyun Zhu, Benoit Chassaing, Rob Knight, David J. Gonzalez, Parambir S. Dulai
Summary: This study investigated the molecular mechanisms of hyperbaric oxygen therapy (HBOT) for moderate-to-severe ulcerative colitis. The findings indicate that HBOT reduces neutrophil STAT3 and azurophilic granule activity in UC patients, and improves colitis activity by altering microbial composition and metabolism. The strain-level variations in A muciniphila in the intestinal microbiota may contribute to the nonresponse to HBOT.
CELLULAR AND MOLECULAR GASTROENTEROLOGY AND HEPATOLOGY
(2022)
Article
Medicine, Research & Experimental
Tatsuya Dokoshi, Jason S. Seidman, Kellen J. Cavagnero, Fengwu Li, Marc C. Liggins, Bryn C. Taylor, Jocelyn Olvera, Rob Knight, John T. Chang, Nita H. Salzman, Richard L. Gallo
Summary: This study revealed that intestinal inflammation can promote the differentiation of intestinal stromal fibroblasts into preadipocytes with innate antimicrobial activity, and this process may be exacerbated by skin inflammation in mice. The activation of hyaluronan catabolism during skin injury was found to alter colon fibroblast function and promote excessive reactive adipogenesis, linking skin and colon inflammation through the release of hyaluronan.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Immunology
Hao Sun, Frederic Lagarrigue, Hsin Wang, Zhichao Fan, Miguel Alejandro Lopez-Ramirez, John T. Chang, Mark H. Ginsberg
Summary: Integrin activation is crucial for lymphocyte trafficking and immune function. RIAM is necessary for conventional T cell integrin activation, but dispensable for regulatory T cell integrin activation and suppressive function. Inhibition of RIAM can prevent spontaneous Tconv cell-mediated autoimmune colitis while maintaining T reg cell trafficking and function.
JOURNAL OF EXPERIMENTAL MEDICINE
(2021)
