Article
Biology
Kirby L. Scott, Nishaka William, Jason P. Acker
Summary: This study evaluates the use of liposomes as a vehicle to internalize trehalose into hematopoietic stem cells (HSCs) and examines its cryoprotective efficacy. The results suggest that the presence of trehalose both inside and outside of the cells significantly improves cell survival.
Article
Physiology
Elizabeth E. Yokum, Matthew Wascher, David L. Goldstein, Carissa M. Krane
Summary: The freeze-tolerant anuran Dryophytes chrysoscelis mobilizes a cryoprotectant system to minimize damage induced by freezing and thawing, including elevated glycerol and glucose levels. Repeated freeze-thaw cycles can lead to glycerol accumulation and glycogen depletion, which delays postfreeze recovery of locomotor function. This research provides insights into the winter survival of D. chrysoscelis under climate change.
AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY
(2023)
Article
Hematology
Anita Howell, Tracey R. Turner, Adele Hansen, Larissa J. Lautner, Qilong Yi, Jason P. Acker
Summary: This study investigated the effect of processing method, additive solution, and storage duration on the quality of cryopreserved red cell concentrates (RCCs) manufactured using the Haemonetics ACP 215. The results suggest that dual centrifugation processing with AS-3 or ESOL additive solutions, with storage periods of up to 21 days both pre-processing and post-deglycerolization, is preferred.
Article
Biochemistry & Molecular Biology
Jing Zhang, Aakriti Gupta, Kenneth B. Storey
Summary: Wood frogs can survive subzero temperatures and confront oxidative stress during freeze/thaw, with Nrf2 playing a key role in antioxidant defenses. High glucose levels affect Nrf2 binding activity but do not stimulate Nrf2 dependent gene expression, with tissue-specific responses observed in antioxidant enzymes such as Prdx2. Our study enhances understanding of the mechanisms behind wood frog freezing survival.
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY B-BIOCHEMISTRY & MOLECULAR BIOLOGY
(2021)
Article
Chemistry, Physical
Xiaomin Xu, Yani Xu, Yuai Li, Min Li, Leilei Wang, Qiang Zhang, Bingjie Zhou, Qing Lin, Tao Gong, Xun Sun, Zhirong Zhang, Ling Zhang
Summary: In this study, a glucose-responsive system mimicking erythrocytes was designed by coupling glucose-responsive nanoparticles to red blood cells, allowing for long-term response to hyperglycemia and potential application in diabetes treatment.
Article
Biochemistry & Molecular Biology
Yu-Chieh Huang, Mei-Ling Cheng, Hsiang-Yu Tang, Chi-Yao Huang, Kuan-Ming Chen, Jong-Shyan Wang
Summary: This study explores the impact of eccentric cycling training (ECT) on erythrocyte antioxidative capacity and metabolism in sedentary males. The results indicate that ECT enhances antioxidative capacity, promotes oxygen release, and mitigates oxidative stress induced by exercise, suggesting the beneficial effects of ECT on overall health and performance.
Article
Chemistry, Applied
Jing-Ru He, Jing-Jing Zhu, Shou-Wei Yin, Xiao-Quan Yang
Summary: This study successfully prepared G/CMC nanoparticles for encapsulating phloretin, improving its stability and bioaccessibility. The nanoparticles showed strong resistance to unfavorable conditions and beneficial effects on human body.
FOOD HYDROCOLLOIDS
(2022)
Article
Endocrinology & Metabolism
Richard C. Wang, Eunice E. Lee, Nicole De Simone, Gauri Kathote, Sharon Primeaux, Adrian Avila, Dong-Min Yu, Mark Johnson, Levi B. Good, Vikram Jakkamsetti, Ravi Sarode, Alice Ann Holland, Juan M. Pascual
Summary: Red blood cells in the brain have close contact with capillary endothelium, providing a pathway for metabolic substrate transfer. Replacing deficient red cells in individuals with glucose transporter I deficiency through exchange transfusion can improve cognitive and quality-of-life measures.
JOURNAL OF CEREBRAL BLOOD FLOW AND METABOLISM
(2023)
Article
Biochemistry & Molecular Biology
Wen Qin, Min Chu, Xiaoming Ma, Jie Pei, Lin Xiong, Pengjia Bao, Yongfu La, Chunnian Liang, Xian Guo, Xiaoyun Wu, Ping Yan
Summary: The changes in semen and cryodamage after cryopreservation negatively affect sperm function and motility. This study compared the proteomes of fresh and frozen-thawed yak sperm using iTRAQ combined with LC-MS/MS proteome approach. A total of 2064 proteins were identified, with 161 showing significant differences between fresh and frozen-thawed sperm. The differentially expressed proteins are mostly associated with spermatogenesis, metabolic pathways, and ATP synthesis. The results indicate that cryopreservation alters the proteome of yak sperm, potentially affecting its fertilization ability.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biology
Monika Nemcova, Veronika Seidlova, Jan Zukal, Heliana Dundarova, Sarka Bednarikova, Jiri Pikula
Summary: The evolution of heterothermy in bats enables them to survive in environments with variable temperatures by using torpor as a hibernation strategy. Cells derived from heterothermic bats can survive sub-zero temperatures and higher glucose levels improve their hibernation survival at extremely low temperatures. Other mechanisms may also contribute to cell survival at low temperatures.
JOURNAL OF THERMAL BIOLOGY
(2023)
Article
Cell Biology
Denisa Dzulova, Dylan Lawless, Gaetan G. Pinton, Nicole A. Renner, Daniel F. Schorderet
Summary: Zebrafish have extraordinary potential for regeneration in multiple organs. This study applied cryoinjury to the zebrafish eye and found that the retina, while undergoing limited tissue remodeling and regrowth, cannot completely recover and develops a persistent dysplasia. RNA sequencing analysis identified potential candidate genes and enriched processes. This zebrafish injury model may be useful for studying critical pathways for successful regeneration.
Article
Multidisciplinary Sciences
Phong D. Nguyen, Iris Gooijers, Giulia Campostrini, Arie O. Verkerk, Hessel Honkoop, Mara Bouwman, Dennis E. M. de Bakker, Tim Koopmans, Aryan Vink, Gerda E. M. Lamers, Avraham Shakked, Jonas Mars, Aat A. Mulder, Sonja Chocron, Kerstin Bartscherer, Eldad Tzahor, Christine L. Mummery, Teun P. de Boer, Milena Bellin, Jeroen Bakkers
Summary: Zebrafish hearts can regenerate by replacing damaged tissue with new cardiomyocytes. The cardiac dyad, a structure that regulates calcium handling and excitation-contraction coupling, plays a key role in the redifferentiation process.
Article
Fisheries
Linlin Li, Yongsheng Tian, Zhentong Li, Ziqi Li, Pengfei Duan, Xinyi Wang, Shuai Chen, Linna Wang, Qingbin Wang, Jieming Zhai
Summary: The study successfully preserved Cromileptes altivelis embryos using non-permeable cryoprotectants for the first time. Trehalose, sucrose, and lactose were found to be less toxic to the embryos compared to fructose and glucose. Combining sucrose and trehalose at a 1:1 ratio resulted in higher survival rates.
Article
Multidisciplinary Sciences
Yosuke Harada, Tomohisa Sujino, Kentaro Miyamoto, Ena Nomura, Yusuke Yoshimatsu, Shun Tanemoto, Satoko Umeda, Keiko Ono, Yohei Mikami, Nobuhiro Nakamoto, Kaoru Takabayashi, Naoki Hosoe, Haruhiko Ogata, Tuneo Ikenoue, Atsushi Hirao, Yoshiaki Kubota, Takanori Kanai
Summary: This study revealed the metabolic properties of intestinal intraepithelial lymphocytes (IELs) and found that the intraepithelial compartment becomes hypoxic in the presence of microbes. Induced CD4(+)CD8 alpha alpha+TCR beta(+) T cells (DPIELs) increase in this location and have lower oxygen and glucose consumption, as well as unique alterations in mitochondria.
Article
Nutrition & Dietetics
Victor Toro-Roman, Jesus Siquier-Coll, Ignacio Bartolome, Francisco J. Grijota, Diego Munoz, Marcos Maynar-Marino
Summary: This study analyzed the influence of physical training on zinc concentrations. The results showed that athletes who underwent regular physical training had elevated plasma zinc concentrations and reduced erythrocyte zinc concentrations.
JOURNAL OF THE INTERNATIONAL SOCIETY OF SPORTS NUTRITION
(2022)
Article
Hematology
Stacy M. Endres-Dighe, Yuelong Guo, Tamir Kanias, Marion Lanteri, Mars Stone, Bryan Spencer, Ritchard G. Cable, Joseph E. Kiss, Steve Kleinman, Mark T. Gladwin, Donald J. Brambilla, Pam D'Andrea, Darrell J. Triulzi, Alan E. Mast, Grier P. Page, Andmichael P. Busch, A. E. Mast, J. L. Gottschall, W. Bialkowski, L. Anderson, J. Miller, A. Hall, Z. Udee, V. Johnson, D. J. Triulzi, J. E. Kiss, P. A. D'Andrea, E. L. Murphy, A. M. Guiltinan, R. G. Cable, B. R. Spencer, S. T. Johnson, D. J. Brambilla, M. T. Sullivan, S. M. Endres, G. P. Page, Y. Guo, N. Haywood, D. Ringer, B. C. Siege, M. P. Busch, M. C. Lanteri, M. Stone, S. Keating, T. Kania, M. Gladwin, S. H. Kleinman, S. A. Glynn, K. B. Malkin, A. M. Cristman
Article
Hematology
Marion C. Lanteri, Tamir Kanias, Sheila Keating, Mars Stone, Yuelong Guo, Grier P. Page, Donald J. Brambilla, Stacy M. Endres-Dighe, Alan E. Mast, Walter Bialkowski, Pam D'Andrea, Ritchard G. Cable, Bryan R. Spencer, Darrell J. Triulzi, Edward L. Murphy, Steven Kleinman, Mark T. Gladwin, Michael P. Busch, A. E. Mast, J. L. Gottschall, W. Bialkowski, L. Anderson, J. Miller, A. Hall, Z. Udee, V. Johnson, D. J. Triulzi, J. E. Kiss, P. A. D'Andrea, E. L. Murphy, A. M. Guiltinan, R. G. Cable, B. R. Spencer, S. T. Johnson, D. J. Brambilla, M. T. Sullivan, S. M. Endres-Dighe, G. P. Page, Y. Guo, N. Haywood, D. Ringer, B. C. Siege, M. P. Busch, M. C. Lanteri, M. Stone, S. Keating, T. Kanias, M. Gladwin, S. H. Kleinman, S. A. Glynn, K. B. Malkin, A. M. Cristman
Article
Hematology
Tamir Kanias, Mars Stone, Grier P. Page, Yuelong Guo, Stacy M. Endres-Dighe, Marion C. Lanteri, Bryan R. Spencer, Ritchard G. Cable, Darrell J. Triulzi, Joseph E. Kiss, Edward L. Murphy, Steve Kleinman, Mark T. Gladwin, Michael P. Busch, Alan E. Mast, A. E. Mast, J. L. Gottschall, W. Bialkowski, L. Anderson, J. Miller, A. Hall, Z. Udee, V. Johnson, D. J. Triulzi, J. E. Kiss, P. A. D'Andrea, E. L. Murphy, A. M. Guiltinan, R. G. Cable, B. R. Spencer, S. T. Johnson, D. J. Brambilla, M. T. Sullivan, S. M. Endres, G. P. Page, Y. Guo, N. Haywood, D. Ringer, B. C. Siege, M. P. Busch, M. C. Lanteri, M. Stone, S. Keating, T. Kanias, M. Gladwin, S. H. Kleinman, S. A. Glynn, K. B. Malkin, A. M. Cristman
Article
Hematology
Mars Stone, Sheila M. Keating, Tamir Kanias, Marion C. Lanteri, Mila Lebedeva, Derek Sinchar, Dylan Hampton, Adam Jakub, Val Rychka, Greg Brewer, Sonia Bakkour, Nelly Gefter, Karla Murcia, Grier P. Page, Stacy Endres-Dighe, Walter Bialkowski, Xiaoyun Fu, Jim Zimring, Thomas J. Raife, Steve Kleinman, Mark T. Gladwin, Michael P. Busch, A. E. Mast, J. L. Gottschall, W. Bialkowski, L. Anderson, J. Miller, A. Hall, Z. Udee, V. Johnson, D. J. Triulzi, J. E. Kiss, P. A. D'Andrea, E. L. Murphy, A. M. Guiltinan, R. G. Cable, B. R. Spencer, S. T. Johnson, D. J. Brambilla, M. T. Sullivan, S. M. Endres, G. P. Page, Y. Guo, N. Haywood, D. Ringer, B. C. Siege, M. P. Busch, M. C. Lanteri, M. Stone, S. Keating, T. Kanias, M. Gladwin, S. H. Kleinman, S. A. Glynn, K. B. Malkin, A. M. Cristman
Article
Hematology
Robert A. DeSimone, Colleen Plimier, Catherine Lee, Tamir Kanias, Melissa M. Cushing, Bruce S. Sachais, Steven Kleinman, Michael P. Busch, Nareg H. Roubinian
Article
Hematology
Angelo D'Alessandro, Xiaoyun Fu, Julie A. Reisz, Tamir Kanias, Grier P. Page, Mars Stone, Steve Kleinman, James C. Zimring, Michael Busch
Article
Hematology
Lorenzo Bertolone, Micaela Kalani Roy, Ariel M. Hay, Evan J. Morrison, Davide Stefanoni, Xiaoyun Fu, Tamir Kanias, Steve Kleinman, Larry J. Dumont, Mars Stone, Travis Nemkov, Michael P. Busch, James C. Zimring, Angelo D'Alessandro
Article
Hematology
Kelsey Hazegh, Marjorie D. Bravo, Hany Kamel, Larry Dumont, Tamir Kanias
Article
Hematology
Davide Stefanoni, Xiaoyun Fu, Julie A. Reisz, Tamir Kanias, Travis Nemkov, Grier P. Page, Larry Dumont, Nareg Roubinian, Mars Stone, Steve Kleinman, Michael Busch, James C. Zimring, Angelo D'Alessandro
Article
Hematology
Keisha Alexander, Kelsey Hazegh, Fang Fang, Derek Sinchar, Joseph E. Kiss, Grier P. Page, Angelo D'Alessandro, Tamir Kanias
Summary: The study revealed significant differences in metabolism and hemolysis susceptibility in stored red blood cells from patients receiving testosterone replacement therapy, showing higher antioxidant pathway activation and enhanced hemolysis susceptibility.
Article
Hematology
Kelsey Hazegh, Bradley D. Anawalt, Larry J. Dumont, Tamir Kanias
Summary: This study found that blood from donors receiving testosterone replacement therapy have significantly higher concentrations of free and total testosterone compared to controls. The concentrations of free testosterone in RBC units were higher, and it is suggested to avoid blood collections soon after testosterone dosing.
Article
Hematology
Albert D. Donnenberg, Daniel B. Kim-Shapiro, Tamir Kanias, Linda R. Moore, Joseph E. Kiss, Janet S. S. Lee, Zeyu Xiong, Ling Wang, Darrell J. Triulzi, Mark T. Gladwin
Summary: By labeling and tracking BioRBC, the study found that fresh and aged BioRBC have similar clearance kinetics, which may be due to extensive washing during biotinylation. This finding suggests that washing and potential RBC culling may extend the storage life of RBC products.
Article
Hematology
Marc Cloutier, Fabrice Cognasse, Ana Paula Hitomi Yokoyama, Kelsey Hazegh, Olga Mykhailova, Mackenzie Brandon-Coatham, Hind Hamzeh-Cognasse, Jose Mauro Kutner, Jason P. Acker, Tamir Kanias
Summary: This study analyzed blood donors at the extremes of the age spectrum and found that teenage donors have higher red blood cell concentration, while older donors are more susceptible to oxidative hemolysis. These findings suggest that age-specific changes in red blood cell antioxidant capacity and physical characteristics may impact the storage and transfusion effectiveness of red blood cells.
Article
Medicine, Research & Experimental
Grier P. Page, Tamir Kanias, Yuelong J. Guo, Marion C. Lanteri, Xu Zhang, Alan E. Mast, Ritchard G. Cable, Bryan R. Spencer, Joseph E. Kiss, Fang Fang, Stacy M. Endres-Dighe, Donald Brambilla, Mehdi Nouraie, Victor R. Gordeuk, Steve Kleinman, Michael P. Busch, Mark T. Gladwin
Summary: Genome-wide association study identified genetic variants in erythrocyte structural and functional genes that influence hemolytic stress during transfusion and disease. These variants may impact erythrocyte recovery after transfusion and modulate disease severity in hemolytic diseases such as sickle cell disease and malaria.
JOURNAL OF CLINICAL INVESTIGATION
(2021)
Article
Hematology
Angelo D'Alessandro, Julie A. Reisz, Yingze Zhang, Sarah Gehrke, Keisha Alexander, Tamir Kanias, Darrell J. Triulzi, Chenell Donadee, Suchitra Barge, Jessica Badlam, Shilpa Jain, Michael G. Risbano, Mark T. Gladwin