Article
Medicine, Research & Experimental
Travis Nemkov, Davide Stefanoni, Aarash Bordbar, Aaron Issaian, Bernhard O. Palsson, Larry J. Dumont, Ariel Hay, Anren Song, Yang Xia, Jasmina S. Redzic, Elan Z. Eisenmesser, James C. Zimring, Steve Kleinman, Kirk C. Hansen, Michael P. Busch, Angelo D'Alessandro
Summary: Computational models suggest that mature erythrocytes may be affected by common drugs. High-throughput drug screenings show that many drugs impact erythrocyte metabolism, with machine learning models accurately predicting these effects. Certain drugs like ranitidine can improve erythrocyte metabolism and storage quality, indicating potential for enhancing blood transfusion outcomes.
Article
Hematology
Madeleine Casimir, Martin Colard, Michael Dussiot, Camille Roussel, Anais Martinez, Carole Peyssonnaux, Patrick Mayeux, Samantha Benghiat, Sandra Manceau, Anne Francois, Nathalie Marin, Frederic Pene, Pierre A. Buffet, Olivier Hermine, Pascal Amireault
Summary: Red blood cell transfusion is an effective treatment for anemic patients, and the efficacy of transfusion is influenced by various factors. This study found a correlation between severe pre-transfusion anemia and a greater than expected increase in hemoglobin levels following transfusion. Additionally, the study suggests that EPO signaling plays a role in improving post-transfusion red blood cell recovery.
AMERICAN JOURNAL OF HEMATOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Tamir Tsohar, Shaul Beyth, Alexander Gural, Dan Arbell, Saul Yedgar, Gregory Barshtein
Summary: This study investigated the impact of non-physiological mechanical stress during surgery on red blood cell deformability. The findings showed that mechanical stress reduces cell deformability, increases the percentage of undeformable cells, and leads to hemolysis of rigid cells. The final outcome depends on the initial concentration of undeformable cells in the sample.
APPLIED SCIENCES-BASEL
(2022)
Article
Surgery
Kasiemobi E. Pulliam, Bernadin Joseph, Amy T. Makley, Charles C. Caldwell, Alex B. Lentsch, Michael D. Goodman, Timothy A. Pritts
Summary: The study showed that washing packed red blood cells during storage can decrease the development of red blood cell storage lesions, reducing microvesicle accumulation and cell-free hemoglobin, with no impact on red blood cell viability.
Article
Biochemistry & Molecular Biology
Kirill A. Kondratov, Alexander A. Artamonov, Vladimir Yu. Mikhailovskii, Anastasiya A. Velmiskina, Sergey V. Mosenko, Evgeniy A. Grigoryev, Anna Yu. Anisenkova, Yuri V. Nikitin, Svetlana V. Apalko, Natalya N. Sushentseva, Andrey M. Ivanov, Sergey G. Scherbak
Summary: This research aimed to investigate erythrocyte morphology abnormalities in COVID-19 patients and compare different poikilocyte types and erythrocyte size dispersion. The study found an increase in acanthocytes and a decrease in spherocytes among COVID-19 patients, as well as an overall increase in erythrocyte size.
Article
Physiology
Abdulrahman Alshalani, Boukje M. Beuger, Anita M. Tuip-de Boer, Robin van Bruggen, Jason P. Acker, Nicole P. Juffermans
Summary: Blood donor characteristics, such as sex, can affect the distribution of young and old red blood cells (RBCs) in circulation. This study found that young RBCs contain more reticulocytes and are associated with lower levels of endothelial activation markers compared to old RBCs. However, RBC subpopulations did not affect T cell proliferation or cytokine responses.
FRONTIERS IN PHYSIOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Annamaria Russo, Ester Tellone, Davide Barreca, Silvana Ficarra, Giuseppina Lagana
Summary: Certain diseases can affect the morphology and function of red blood cells, leading to hematological changes. Monitoring these changes is crucial for understanding the severity of the disease and preventing the progression to dangerous conditions.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Medicine, General & Internal
Piotr F. Czempik, Dawid Gierczak, Dawid Wilczek, Lukasz J. Krzych
Summary: The study analyzed the impact of red blood cell transfusion (RBCT) on blood lactate and the factors that may increase its concentration. It found that RBCT did not normalize mildly elevated lactate and common causes of elevated lactate had no impact on its concentration. Therefore, lactate may have a limited role as a physiologic RBCT trigger in non-bleeding severely anemic critically ill patients.
JOURNAL OF CLINICAL MEDICINE
(2022)
Article
Cell Biology
Brian S. Ferguson, Leslie E. Neidert, Matthew J. Rogatzki, Keith R. Lohse, L. Bruce Gladden, Heidi A. Kluess
Summary: The study found no significant difference in the release of ATP from RBCs of different densities under hypoxic and normoxic conditions, and the release of ATP was linearly correlated with the release of Hb.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Evros Vassiliou, Renalison Farias-Pereira
Summary: Lipids play a crucial role in modulating macrophage functions through intricate signaling pathways. This review focuses on the relationship between lipid metabolism and macrophage functions, and explores potential molecular targets for macrophage-related diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Jeffrey M. Stolwijk, Jonathan A. Stefely, Mike T. Veling, Thomas J. van t Erve, Brett A. Wagner, Thomas J. Raife, Garry R. Buettner
Summary: The research revealed that GPx4 is an active enzyme in mature RBCs, protecting them from hemolysis during blood bank storage. It is correlated with lipid-anchored proteins and anti-correlated with lyso-phospholipids and complement system proteins.
Article
Biochemistry & Molecular Biology
Maciej Bosek, Tomasz Wybranowski, Marta Napiorkowska-Mastalerz, Jerzy Pyskir, Michal Cyrankiewicz, Malgorzata Pyskir, Marta Pilaczynska-Cemel, Alicja Szolna-Chodor, Mateusz Wrembel, Stefan Kruszewski, Grzegorz Przybylski
Summary: COVID-19 can lead to alterations in blood rheology, including an increase in red blood cell aggregation. This study investigates the cellular factors influencing these changes and suggests that erythrocyte aggregation can lead to the formation of denser aggregates, increasing the risk of thrombotic events. It also proposes that cellular aggregation factors may be responsible for thrombotic disorders observed long after infection.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Hematology
Tatsuro Yoshida, Emma McMahon, Harry Croxon, Andrew Dunham, Peter Gaccione, Babak Abbasi, Neil Beckman, Laurel Omert, Stephen Field, Allison Waters
Summary: Oxidative stress is a major factor in the development of storage lesion in red cell concentrates. Donated blood shows wide variability in oxygen saturation at the time of donation, with sex and processing method having minimal effects. A storage simulation model demonstrates a gradual increase in oxygen saturation over 6 weeks, while the oxygen exposure index increases at a faster rate.
Article
Veterinary Sciences
Barbara Steblaj, Jasmin Galli, Paul Torgerson, Annette Kutter
Summary: The aim of this retrospective study was to investigate the association between leukoreduction of packed red blood cell units and transfusion reactions in dogs. The results showed no correlation between leukoreduction and reduction in transfusion reactions or better outcomes.
FRONTIERS IN VETERINARY SCIENCE
(2023)
Article
Medicine, General & Internal
Rui Zhong, Xiaodong Wu, Zhijuan Liu, Zeng He, Xuejun Zhang, Jiaxin Liu, Ye Cao, Hong Wang
Summary: This study investigated the in vitro quality of suspended red blood cells (SRBCs) prepared from Tibetan residents with varying hemoglobin concentrations. The results showed a negative correlation between platelet count and hemoglobin concentration, while electrolyte and lactate, glucose, and 2,3-DPG levels showed positive or negative correlations with hemoglobin concentration. These findings have important implications for the study of storage lesions in high-altitude-induced SRBCs.
FRONTIERS IN MEDICINE
(2023)
Letter
Hematology
Micaela Kalani Roy, Rebecca Burgoyne Wilkerson, Keisha Alexander, Natalie J. Nokoff, Melanie Cree-Green, Angelo D'Alessandro
Article
Critical Care Medicine
Margot DeBot, Christopher Erickson, Marguerite Kelher, Terry R. Schaid, Ernest E. Moore, Angela Sauaia, Alexis Cralley, Ian LaCroix, Angelo D'Alessandro, Kirk Hansen, Mitchell J. Cohen, Christopher C. Silliman, Julia Coleman
Summary: By studying the sex differences in platelets and cryoprecipitate coagulation factors, it was found that blood products from female donors are more effective in improving coagulation dysfunction compared to male blood products. Additionally, female-donor cryoprecipitate contains more factor V and factor XIII, and several proteins with potential immunological significance. These sex differences may influence how transfusions modulate thromboinflammation caused by trauma, suggesting the potential role of sex-specific transfusion guidelines in hemostatic resuscitation.
JOURNAL OF TRAUMA AND ACUTE CARE SURGERY
(2023)
Article
Critical Care Medicine
Terry R. Schaid, Ian LaCroix, Kirk C. Hansen, Angelo D'Alessandro, Ernest E. Moore, Angela Sauaia, Monika Dzieciatkowska, Margot DeBot, Alexis L. Cralley, Otto Thielen, William Hallas, Christopher Erickson, Sanchayita Mitra, Anirban Banerjee, Kenneth Jones, Christopher C. Silliman, Mitchell J. Cohen
Summary: This study identified the proteomic signature of trauma-induced NETosis and revealed that NETosis may mediate organ dysfunction following severe injury. SerpinB1 was identified as a major component of this NET protein profile and could serve as an early marker of excessive NETosis after injury.
JOURNAL OF TRAUMA AND ACUTE CARE SURGERY
(2023)
Article
Hematology
Angelo D'Alessandro, S. Mehdi Nouraie, Yingze Zhang, Francesca Cendali, Fabia Gamboni, Julie A. Reisz, Xu Zhang, Kyle W. Bartsch, Matthew D. Galbraith, Victor R. Gordeuk, Mark T. Gladwin
Summary: This study evaluates the red blood cell metabolism of 587 sickle cell disease patients and finds significant metabolic alterations in RBC acylcarnitines, pyruvate, sphingosine 1-phosphate, creatinine, kynurenine, and urate in Hb SS genotype compared to AA and SC RBCs. Surprisingly, the metabolism of SC RBCs is dramatically different from SS RBCs, with all glycolytic intermediates significantly elevated. These findings provide insights into the metabolic signatures and potential underlying mechanisms in sickle cell disease.
AMERICAN JOURNAL OF HEMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Ji Young Lee, Reece P. Stevens, Viktoriya V. Pastukh, Viktor M. Pastukh, Natalya Kozhukhar, Mikhail F. Alexeyev, Julie A. Reisz, David Nerguizian, Angelo D'Alessandro, Anna Koloteva, Meredith S. Gwin, Justin T. Roberts, Glen M. Borchert, Brant M. Wagener, Jean-Franc Comma Ois Pittet, Brian B. Graham, Kurt R. Stenmark, Troy Stevens
Summary: Pulmonary microvascular endothelial cells prefer glucose over fructose for glycolysis, and this study found that the enzyme PFKFB3 inhibits fructose metabolism in these cells. PFKFB3 knockout led to increased survival and enhanced fructose utilization in fructose-rich medium. The study also demonstrated that pneumonia is associated with increased fructose levels in the lung. These findings suggest that PFKFB3 is a key regulator of glucose versus fructose use in glycolysis in pulmonary microvascular endothelial cells.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2023)
Article
Oncology
Miles Piper, Maureen Hoen, Laurel B. Darragh, Michael W. Knitz, Diemmy Nguyen, Jacob Gadwa, Greta Durini, Idil Karakoc, Abby Grier, Brooke Neupert, Benjamin Van Court, Khalid N. M. Abdelazeem, Justin Yu, Nicholas A. Olimpo, Sophia Corbo, Richard Blake Ross, Tiffany T. Pham, Molishree Joshi, Ross M. Kedl, Anthony J. Saviola, Maria Amann, Pablo Umana, Laura Codarri Deak, Christian Klein, Angelo D'Alessandro, Sana D. Karam
Summary: In PDAC patients, the response to RT involves changes in IL-2Rb, IL-2Rg, and IL-2Ra expression. PD1-IL2v, a PD-1-targeted IL-2 variant immunocytokine, enhances tumor-specific T cell activation and reduces Treg suppression. In PDAC mouse models, PD1-IL2v treatment improves survival, increases tumor-infiltrating CD8+ T cells, and activates antigen-specific CD8+ T cells, especially when combined with single-dose RT.
Article
Immunology
Adam C. Levine, Yuriko Fukuta, Moises A. Huaman, Jiangda Ou, Barry R. Meisenberg, Bela Patel, James H. Paxton, Daniel F. Hanley, Bart J. A. Rijnders, Arvind Gharbharan, Casper Rokx, Jaap Jan Zwaginga, Andrea Alemany, Oriol Mitja, Dan Ouchi, Pere Millat-Martinez, Valerie Durkalski-Mauldin, Frederick K. Korley, Larry J. Dumont, Clifton W. Callaway, Romina Libster, Gonzalo Perez Marc, Diego Wappner, Ignacio Esteban, Fernando Polack, David J. Sullivan
Summary: COVID-19 convalescent plasma treatment can reduce the risk of hospitalization, especially when administered within 5 days of symptom onset and with higher antibody titers.
CLINICAL INFECTIOUS DISEASES
(2023)
Article
Biochemistry & Molecular Biology
Giulia Unali, Giovanni Crivicich, Isabel Pagani, Monah Abou-Alezz, Filippo Folchini, Erika Valeri, Vittoria Matafora, Julie A. Reisz, Anna Maria Sole Giordano, Ivan Cuccovillo, Giacomo M. Butta, Lorena Donnici, Angelo D'Alessandro, Raffaele De Francesco, Lara Manganaro, Davide Cittaro, Ivan Merelli, Carolina Petrillo, Angela Bachi, Elisa Vicenzi, Anna Kajaste-Rudnitski
Summary: The interferon-induced transmembrane proteins (IFITM) inhibit endocytic viral entry by binding to phosphatidylinositol 3,4,5-trisphosphate (PIP3), which plays a key role in endosomal antiviral immunity. The level of PIP3 is closely correlated with the potency of endosomal IFITM restriction, and exogenous PIP3 enhances the inhibition of endocytic viruses. These findings elucidate cell-compartment-specific antiviral mechanisms and have potential relevance for the development of broadly acting antiviral strategies.
Article
Biochemistry & Molecular Biology
Sachin Kumar, Jeffrey D. Vassallo, Kalpana J. Nattamai, Aishlin Hassan, Rebekah Karns, Angelika Vollmer, Karin Soller, Vadim Sakk, Mehmet Sacma, Travis Nemkov, Angelo D'Alessandro, Hartmut Geiger
Summary: It has been found that culturing hematopoietic stem cells (HSCs) at a pH of 6.9 for 2 days can maintain their potential, unlike cultivation at the commonly used pH of 7.4. These HSCs maintained at pH 6.9 exhibit smaller size, lower metabolic activity, weaker proliferation, and enhanced reconstitution ability upon transplantation compared to those cultivated at pH 7.4. This is due to the attenuation of the polyamine pathway in HSCs cultured at pH 6.9. Inhibiting the polyamine pathway in HSCs cultured at pH 7.4 with DFMO mimics the phenotypes and potential of HSCs cultured at pH 6.9. Exposing HSCs to a pH of 6.9 ex vivo positively regulates their function by reducing polyamines. These findings have the potential to improve short-term cultivation protocols for HSC transplantation and gene therapy interventions.
Article
Multidisciplinary Sciences
Jacqueline A. Turner, Malia A. Fredrickson, Marc D'Antonio, Elizabeth Katsnelson, Morgan MacBeth, Robert Van Gulick, Tugs-Saikhan Chimed, Martin McCarter, Angelo D'Alessandro, William A. Robinson, Kasey L. Couts, Roberta Pelanda, Jared Klarquist, Richard P. Tobin, Raul M. Torres
Summary: Lysophosphatidic acid (LPA) increases in concentration in various cancer types and has an impact on CD8 T cell metabolism, phenotype, and effector functions. Plasma concentrations of LPA can predict the response to immunotherapy.
NATURE COMMUNICATIONS
(2023)
Article
Physiology
Francesca I. Cendali, Travis Nemkov, Christina Lisk, Ian S. Lacroix, Seyed-Mehdi Nouraie, Yingze Zhang, Victor R. Gordeuk, Paul W. Buehler, David Irwin, Angelo D'Alessandro
Summary: This study investigated the exercise intolerance and metabolic changes in sickle cell disease patients using a mouse model and clinical cases. The results identified specific metabolites, including carboxylic acids, sphingosine 1-phosphate, and acylcarnitine, that were associated with reduced exercise tolerance in both mouse models and patients. These findings have important implications for understanding the mechanisms of exercise intolerance and developing preventive and therapeutic strategies for sickle cell disease.
FRONTIERS IN PHYSIOLOGY
(2023)
Article
Endocrinology & Metabolism
Shanlee M. Davis, Rhianna Urban, Angelo D'Alessandro, Julie A. Reisz, Christine L. Chan, Megan Kelsey, Susan Howell, Nicole Tartaglia, Philip Zeitler, Peter Baker Ii
Summary: Conditions related to cardiometabolic disease, including metabolic syndrome and type 2 diabetes, are common among men with Klinefelter syndrome (KS). The molecular mechanisms underlying this aberrant metabolism in KS are largely unknown. This study found that the plasma metabolome profile in adolescent males with KS is distinctly different from that in males without KS, suggesting differences in mitochondrial β-oxidation.
ENDOCRINE CONNECTIONS
(2023)
Article
Cell Biology
Eric. L. L. Bolf, Thomas. C. C. Beadnell, Madison. M. M. Rose, Angelo D'Alessandro, Travis Nemkov, Kirk. C. C. Hansen, Rebecca. E. E. Schweppe
Summary: Thyroid cancer is a common endocrine neoplasm with a poor prognosis for patients with metastatic disease or resistance to radioactive iodine. This study examines the effects of kinase inhibitors on thyroid cancer cells and reveals significant alterations in metabolite profiles, particularly in glycolysis, the TCA cycle, and amino acid levels. The study also highlights the accumulation of the tumor-suppressive metabolite 2-oxoglutarate and its potential in reducing thyroid cancer cell viability. These findings emphasize the importance of understanding how therapeutics reprogram metabolic processes and cancer cell behavior.
Article
Developmental Biology
Hiroko Kawakami, Katherine Q. Chen, Ruizhi Zhang, Matthew P. Pappas, Abigail Bailey, Julie A. Reisz, Dylan Corcoran, Ryuichi Nishinakamura, Angelo D'Alessandro, Yasuhiko Kawakami
Summary: Recent studies have shown that regulation of cellular metabolism, especially glycolysis and pathways branching from glycolysis, plays a crucial role in vertebrate embryo development. The transcription factor Sall4 is highly expressed in undifferentiated cells in developing mouse embryos and is involved in the regulation of glycolytic metabolism. Knockout experiments demonstrate that Sall4 restricts glycolysis in limb buds and is important for hindlimb patterning.
DEVELOPMENTAL BIOLOGY
(2023)
Article
Nutrition & Dietetics
Christopher Y. Kim, Hannah J. Larsen, Steven L. Spitalnik, Eldad A. Hod, Richard O. Francis, Krystalyn E. Hudson, Dominique E. Gordy, Elizabeth F. Stone, Sandy Peltier, Pascal Amireault, Angelo D'Alessandro, James C. Zimring, Paul W. Buehler, Xiaoyun Fu, Tiffany Thomas
Summary: High-dose fish oil intake reduces the quality of red blood cells, while low-dose fish oil improves the filterability of fresh red blood cells and reduces lipid peroxidation of stored red blood cells. However, low-dose fish oil does not improve the post-transfusion recovery capacity of stored red blood cells.