Review
Cell Biology
Jocelynda Salvador, M. Luisa Iruela-Arispe
Summary: Vascular cells are constantly influenced by physical forces associated with heart activities and vessel geometry, which regulate the transcriptional program and phenotype of these cells in different regions of the vascular tree. The nucleus plays a critical role as a mechanosensor, and maintaining nuclear integrity is essential for proper vascular function and stability.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Mehwish Arshad, Mean Ghim, Yumnah Mohamied, Spencer J. Sherwin, Peter D. Weinberg
Summary: The study rigorously tested the alignment of arterial endothelial cells with wall shear stress (WSS) vector, finding that at high mean WSS magnitudes, cells aligned to minimize shear across their long axis instead of aligning with the mean WSS vector. This suggests that other aspects of endothelial behavior attributed to WSS metric may need re-examination, and the evolution of a mechanism to minimize transverse WSS could have detrimental effects on cells and play a role in atherogenesis.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2021)
Article
Biophysics
Andrea Alonso, Alessandra Ebben, Mahsa Dabagh
Summary: The objective of this study is to demonstrate how mechanical signaling in subcellular organelles of endothelial cells (ECs) is affected by changes in blood flow profiles and arterial wall properties. The results of the study indicate that arterial stiffening and different flow profiles alter mechanotransduction in the intra/inter-cellular organelles of ECs, leading to changes in stress transmission.
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2023)
Article
Hematology
Dongyang Jiang, Hao Liu, Guofu Zhu, Xiankai Li, Linlin Fan, Faxue Zhao, Chong Xu, Shumin Wang, Yara Rose, Jordan Rhen, Ze Yu, Yiheng Yin, Yuling Gu, Xiangbin Xu, Edward A. Fisher, Junbo Ge, Yawei Xu, Jinjiang Pang
Summary: In this study, researchers identified the proatherosclerotic effect of endothelial PHACTR1, contrary to macrophage PHACTR1. They demonstrated that global or endothelial cell specific Phactr1 deficiency can significantly inhibit atherosclerosis. Further research revealed that PHACTR1 functions as a PPARγ transcriptional corepressor by binding to PPARγ, which regulates endothelial activation and atherosclerosis. The findings suggest that endothelial PHACTR1 is a potential therapeutic target for atherosclerosis treatment.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2023)
Review
Cell Biology
Hui Lin Zhou, Xi Zhuo Jiang, Yiannis Ventikos
Summary: This review summarizes the research on different blood fluid patterns and fluid shear stress in the circulation system, as well as the interactions between blood flow and endothelial cells. It highlights the role of the endothelial glycocalyx in maintaining endothelial health and discusses strategies for restoring damaged glycocalyx. This review provides a new perspective on the role of blood flow in regulating endothelial functionality.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Review
Cell Biology
Mohammad Hamrangsekachaee, Ke Wen, Sidi A. Bencherif, Eno E. Ebong
Summary: Endothelium health is crucial for regulating physiological vascular functions. Dysfunction of endothelial cells (ECs) is associated with various vascular diseases, especially atherosclerosis and cardiovascular diseases. This review discusses mechanotransduction events mediated by ECs, the involved subcellular components, and potential pathways. It also reviews current research efforts using in vivo animal models and in vitro biomimetic models, along with recommendations for future modeling approaches to aid the development of novel therapies targeting atherosclerosis and related cardiovascular diseases.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Cell Biology
Matthew Rickman, Mean Ghim, Kuin Pang, Ana Cristina von Huelsen Rocha, Elena M. Drudi, Macia Sureda-Vives, Nicolas Ayoub, Virginia Tajadura-Ortega, Sarah J. George, Peter D. Weinberg, Christina M. Warboys
Summary: Multidirectional or disturbed flow promotes endothelial dysfunction and early atherogenesis. This study revealed the involvement of Wnt signaling in flow-mediated endothelial dysfunction. The expression of Frizzled-4 increased in ECs exposed to disturbed flow, with the knockdown of R-spondin-3 abolishing this increase. Disturbed flow also led to nuclear localization and activation of D-catenin, dependent on Frizzled-4 and R-spondin-3. Inhibition of D-catenin, Frizzled-4, or R-spondin-3, as well as WNT5A signaling, reduced the expression of pro-inflammatory genes in ECs exposed to disturbed flow. Inhibition of the canonical Wnt pathway had no effect. Inhibiting D-catenin also reduced endothelial paracellular permeability through altered junctional and focal adhesion organization and cytoskeletal remodeling.
JOURNAL OF CELL SCIENCE
(2023)
Review
Medicine, Research & Experimental
Hongxin Cheng, Wen Zhong, Lu Wang, Qing Zhang, Xiaoqing Ma, Yang Wang, Shiqi Wang, Chengqi He, Quan Wei, Chenying Fu
Summary: Different blood flow patterns in arteries alter the phenotype of vascular endothelial cells, affecting their functions and directly contributing to the occurrence of early atherosclerosis lesions. Oscillating shear stress (OSS) in curved or bifurcated arteries promotes the transformation of endothelial cells into pro-inflammatory phenotypes, leading to increased inflammation, oxidative stress, mitochondrial dysfunction, metabolic abnormalities, and enhanced endothelial permeability, thus promoting atherosclerosis progression. In contrast, stable laminar shear stress (LSS) in straight arteries facilitates the transformation of endothelial cells into an anti-inflammatory phenotype, improving their function and inhibiting atherosclerotic progression. This article outlines the relationship between vascular endothelial cell functions and different forms of fluid shear stress in atherosclerosis and explores potential therapeutic approaches targeting intracellular mechanical transductions.
BIOMEDICINE & PHARMACOTHERAPY
(2023)
Review
Geriatrics & Gerontology
Danyuan Huang, Shuo Chen, Ding Xiong, Han Wang, Li Zhu, Yuanyuan Wei, Yuyu Li, Shujuan Zou
Summary: Cells need to sense and adapt to mechanical changes in their environment. Mitochondrial dynamics and the cytoskeleton play crucial roles in this process. However, the mechanisms underlying the integration of mechanosensing, mechanotransduction, and metabolic reprogramming remain poorly understood.
Article
Multidisciplinary Sciences
Claire Leclech, David Gonzalez-Rodriguez, Aurelien Villedieu, Thevy Lok, Anne-Marie Deplanche, Abdul I. Barakat
Summary: The emergence of a specific pattern of collective cell migration in unconfined endothelial monolayers on microgroove substrates has been demonstrated. This pattern exhibits periodic antiparallel cell streams, which are dependent on intact cell-cell junctions and sensitive to groove depth.
NATURE COMMUNICATIONS
(2022)
Article
Engineering, Biomedical
Mark Colasurdo, Elisa B. Nieves, Marc A. Fernandez-Yague, Christian Franck, Andres J. Garcia
Summary: Cell-extracellular matrix forces play a crucial role in regulating various physiological and pathological processes. However, the study of mechanobiology in three-dimensional systems is still limited. In this study, a platform integrating a synthetic hydrogel system with 3D traction force microscopy was developed to evaluate deformation and force responses within synthetic microenvironments. The findings shed light on the contributions of adhesive peptide density and polymer density, as well as matrix stiffness, to 3D force generation by fibroblasts. Rho-kinase-dependent contractility and vinculin expression were also found to be essential for significant 3D force generation. This research provides valuable insights into mechanobiology and how cells sense and transmit forces in three dimensions.
Article
Biochemistry & Molecular Biology
Ahmad Chehaitly, Anne-Laure Guihot, Coralyne Proux, Linda Grimaud, Jade Aurriere, Benoit Legouriellec, Jordan Rivron, Emilie Vessieres, Clement Tetaud, Antonio Zorzano, Vincent Procaccio, Francoise Joubaud, Pascal Reynier, Guy Lenaers, Laurent Loufrani, Daniel Henrion
Summary: The reduction in mitochondrial fusion in mouse endothelial cells was found to impair the dilator response to shear stress, leading to excessive superoxide production and promoting greater atherosclerosis development.
Article
Cell Biology
Guozhang He, Shuang Kan, Shaohua Xu, Xuchen Sun, Rong Li, Wei Shu, Ming Chen
Summary: In this study, the researchers found that the expression of LXN in ECs is significantly influenced by laminar shear stress, and LXN knockdown can lead to changes in cell morphology and cytoskeleton remodeling, improving vascular damage and atherosclerosis in mice. This provides new strategies and drug targets for the treatment of vascular diseases.
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2021)
Article
Multidisciplinary Sciences
Lydia Djenoune, Mohammed Mahamdeh, Thai V. Truong, Christopher T. Nguyen, Scott E. Fraser, Martina Brueckner, Jonathon Howard, Shiaulou Yuan
Summary: The breaking of bilateral symmetry in most vertebrates relies on the motile cilia of the embryonic left-right organizer (LRO), which generate fluid flow. This study demonstrated that the immotile LRO cilia serve as mechanosensors for shear force in zebrafish. Mechanical force applied to immotile LRO cilia activated intraciliary calcium transients, requiring the cation channel Polycystin-2. The study also showed that mechanical force applied to LRO cilia could rescue and reverse cardiac situs in zebrafish lacking motile cilia.
Review
Biochemistry & Molecular Biology
Katharina Urschel, Miyuki Tauchi, Stephan Achenbach, Barbara Dietel
Summary: Atherosclerosis development is influenced not only by high cholesterol but also by hemodynamic forces that affect endothelial cell function. Studying the impact of wall shear stress can help understand the mechanisms of atherosclerosis and prevent related heart diseases.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Meeting Abstract
Biophysics
Lionel Guillou, Avin Babataheri, Pierre-Henri Puech, Abdul Barakat, Julien Husson
BIOPHYSICAL JOURNAL
(2016)
Article
Biophysics
David Gonzalez-Rodriguez, Lionel Guillou, Francois Cornat, Julie Lafaurie-Janvore, Avin Babataheri, Emmanuel de Langre, Abdul I. Barakat, Julien Husson
BIOPHYSICAL JOURNAL
(2016)
Article
Peripheral Vascular Disease
Edouard Lecarpentier, Anthony Atallah, Jean Guibourdenche, Marylise Hebert-Schuster, Sarah Vieillefosse, Audrey Chissey, Bassam Haddad, Guillaume Pidoux, Daniele Evain-Brion, Abdul Barakat, Thierry Fournier, Vassilis Tsatsaris
Article
Multidisciplinary Sciences
Elizabeth E. Antoine, Francois P. Cornat, Abdul I. Barakat
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2016)
Article
Cell Biology
Lionel Guillou, Avin Babataheri, Michael Saitakis, Armelle Bohineust, Stephanie Dogniaux, Claire Hivroz, Abdul I. Barakat, Julien Husson
MOLECULAR BIOLOGY OF THE CELL
(2016)
Article
Cell Biology
Anna Sawicka, Avin Babataheri, Stephanie Dogniaux, Abdul I. Barakat, David Gonzalez-Rodriguez, Claire Hivroz, Julien Husson
MOLECULAR BIOLOGY OF THE CELL
(2017)
Article
Multidisciplinary Sciences
Anne-Clemence Vion, Marouane Kheloufi, Adel Hammoutene, Johanne Poisson, Juliette Lasselin, Cecile Devue, Isabelle Pic, Nicolas Dupont, Johanna Busse, Konstantin Stark, Julie Lafaurie-Janvore, Abdul I. Barakat, Xavier Loyer, Michele Souyri, Benoit Viollet, Pierre Julia, Alain Tedgui, Patrice Codogno, Chantal M. Boulanger, Pierre-Emmanuel Rautou
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2017)
Article
Cardiac & Cardiovascular Systems
Kazuyuki Yahagi, Yi Yang, Sho Torii, Johanne Mensah, Roseann M. White, Marion Mathieu, Erica Pacheco, Masataka Nakano, Abdul Barakat, Tahmer Sharkawi, Michel Vert, Michael Joner, Aloke V. Finn, Renu Virmani, Antoine Lafont
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2017)
Editorial Material
Cell Biology
Marouane Kheloufi, Anne-Clemence Vion, Adel Hammoutene, Johanne Poisson, Juliette Lasselin, Cecile Devue, Isabelle Pic, Nicolas Dupont, Johanna Busse, Konstantin Stark, Julie Lafaurie-Janvore, Abdul I. Barakat, Xavier Loyer, Michele Souyri, Benoit Viollet, Pierre Julia, Alain Tedgui, Patrice Codogno, Chantal M. Boulanger, Pierre-Emmanuel Rautou
Article
Mathematics, Applied
Ezio Di Costanzo, Abdul I. Barakat, Giuseppe Pontrelli
ZAMM-ZEITSCHRIFT FUR ANGEWANDTE MATHEMATIK UND MECHANIK
(2018)
Article
Multidisciplinary Sciences
V. Chandran Suja, A. I. Barakat
SCIENTIFIC REPORTS
(2018)
Article
Biophysics
Hengdi Zhang, Zaiyi Shen, Brenna Hogan, Abdul Barakat, Chaouqi Misbah
BIOPHYSICAL JOURNAL
(2018)
Article
Mathematics, Applied
Ezio Di Costanzo, Abdul I. Barakat, Giuseppe Pontrelli
ZAMM-ZEITSCHRIFT FUR ANGEWANDTE MATHEMATIK UND MECHANIK
(2018)
Article
Biophysics
Brenna Hogan, Zaiyi Shen, Hengdi Zhang, Chaouqi Misbah, Abdul I. Barakat
BIOMECHANICS AND MODELING IN MECHANOBIOLOGY
(2019)
Article
Multidisciplinary Sciences
C. F. Natale, J. Lafaurie-Janvore, M. Ventre, A. Babataheri, A. Barakat
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2019)
