Article
Genetics & Heredity
Fatin Ahmad Rizal, Kok Lian Ho, Abdul Rahman Omar, Wen Siang Tan, Abdul Razak Mariatulqabtiah, Munir Iqbal
Summary: This study compared the molecular markers of a locally isolated LPAI AIV strain H5N2 from Malaysia with reference HPAI strains, revealing distinct characteristics of the Malaysian strain and its potential role in the epidemiological research on circulating AIV in poultry.
Article
Veterinary Sciences
Amanda L. Skarlupka, Ted M. Ross
Summary: Research has shown that raw animal sera can inhibit a range of influenza virus neuraminidase (NA), but the breadth and effectiveness of inhibition depend on the source of sera. Despite different sources of sera exhibiting varying levels of inhibition against different influenza viruses, further studies are needed to ensure consistency of scientific methods in order to support the development of broadly protective influenza virus vaccines.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Virology
Lorlane Le Targa, Houmadi Hikmat, Celine Boschi, Bernard La Scola, Philippe Colson
Summary: This study aimed to assess the association between genetic mutations in influenza viruses and false-negative results of qPCR detection. Analysis of 624 A/H3N2 influenza virus genomes revealed widespread presence of A37T/C and C161T mutations, as well as other mutations, in qPCR-positive samples.
Article
Biotechnology & Applied Microbiology
Daniel Lindhorst, Marc S. Halfon
Summary: The overlap between activity-based and chromatin-based definitions of transcriptional enhancers is limited, suggesting the need for new approaches to define enhancers. The study reveals the poor agreement among existing methods and highlights the importance of reconciling the discrepancies in enhancer definition.
Article
Cell Biology
De-Jian Liu, Cui-Cui Liu, Xiu-Qin Zhong, Xuan Wu, Hui-Hui Zhang, Shang-Wen Lu, Zhuo-Ling Shen, Wen-Wen Song, Shi-Long Zhao, You-Song Peng, He-Ping Zheng, Mu-Yang Wan, Yao-Qing Chen, Lei Deng
Summary: Neuraminidase is identified as a crucial element in the development of a universal influenza vaccine. Inducing neuraminidase-specific broadly protective antibodies through vaccinations is difficult. In this study, conserved peptides from the globular head domains of neuraminidase were selected to focus the immune response and enhance cross-protections. The peptide-based sequential immunization strategy proved to be effective in inducing targeted and cross-protective antibody response, providing insights for designing universal vaccines against highly variable pathogens.
Article
Biochemistry & Molecular Biology
Masa Kenda, Jan Vegelj, Barbara Herlah, Andrej Perdih, Premysl Mladenka, Marija Sollner Dolenc
Summary: The study evaluated the inhibition of firefly luciferase by isoflavonoids, finding that most of these compounds have varying degrees of inhibition on firefly luciferase but not on Renilla luciferase. This suggests the importance of reporter-enzyme inhibition when studying the effects of such compounds, and the in vitro assay used can help exclude false-positives due to reporter gene inhibition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Busra Kaya, Ferdane Danisman Kalindemirtas, Onur Ertik, Refiye Yanardag, Serap Erdem Kuruca, Bahri Ulkueseven
Summary: New thiosemicarbazone-based zinc(II) complexes were synthesized and their cytotoxicity on A375 malignant melanoma cells as well as the effect on normal cells was studied. The complex with a 3-methoxy-salicylidene moiety exhibited high cytotoxicity against A375 cancer cells, surpassing the effectiveness of cisplatin. The complexes were also found to inhibit neuraminidase enzyme, which might contribute to their cytotoxicity.
CHEMICO-BIOLOGICAL INTERACTIONS
(2022)
Article
Pharmacology & Pharmacy
Abdul Bari Shah, Aizhamal Baiseitova, Jeong Ho Kim, Yong Hyun Lee, Ki Hun Park
Summary: This study reveals the neuraminidase inhibitory potential of metabolites from Helminthostachys zeylanica, which can also inhibit biofilm formation, leading to potential applications in microbial disease treatment.
FRONTIERS IN PHARMACOLOGY
(2022)
Article
Pharmacology & Pharmacy
Yanni Lai, Tiantian Han, Shaofeng Zhan, Yong Jiang, Xiaohong Liu, Geng Li
Summary: Isoimperatorin, a natural compound, exhibits broad-spectrum antiviral activity against various influenza virus strains, especially showing effective inhibition during the later stages of the virus replication cycle. It also exerts inhibitory effects on neuraminidase-mediated virus release, making it a potential agent for influenza A virus prevention and treatment.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Daniel Musikant, Romina Higa, Cristina E. Rodriguez, Martin M. Edreira, Oscar Campetella, Alicia Jawerbaum, Maria S. Leguizam
Summary: In infections caused by Trypanosoma cruzi, different strains of the parasite can modulate the levels of circulating matrix metalloproteinases (MMPs), affecting their activity in blood. Furthermore, the virulence factor trans-sialidase is involved in regulating MMP-2 activity by removing sialic acid residues from cell surfaces and triggering specific signaling pathways. This novel pathogenic mechanism may be exploited by other pathogens expressing similar sialidases to regulate the MMP network.
Article
Biochemistry & Molecular Biology
Seyed Arad Moghadasi, Sofia N. Moraes, Reuben S. Harris
Summary: The study developed a two-color flow cytometry-based assay that enables sensitive quantification of DUB activity and inhibition in living cells, providing a potential tool for studying DUB biology in a cellular context and aiding in inhibitor discovery and development.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Review
Endocrinology & Metabolism
Digne Tembely, Auberi Henry, Laetitia Vanalderwiert, Kevin Toussaint, Amar Bennasroune, Sebastien Blaise, Herve Sartelet, Stephane Jaisson, Celine Gales, Laurent Martiny, Laurent Duca, Beatrice Romier-Crouzet, Pascal Maurice
Summary: The incidence of cardiovascular diseases is increasing due to the aging population, which has a major influence on the vascular tree. Aging leads to changes in the morphology and function of the arterial wall, as well as remodeling of the vascular extracellular matrix. The release of elastin-derived peptides (EDPs) from elastin degradation products is a characteristic feature of aged conduit arteries. These EDPs not only affect the mechanical properties of arteries but also modulate the development and progression of various vascular and metabolic diseases. The elastin receptor complex (ERC), which contains elastin-binding protein, protective protein/cathepsin A, and neuraminidase-1 (NEU1), plays a crucial role in mediating the biological effects of these peptides. This review provides an overview of the consequences of aging on the vasculature and the release of EDPs, discusses the composition and signaling pathways of the ERC, and explores the pharmacological strategies targeting ERC activation. Additionally, it highlights the emerging regulatory functions of the ERC through desialylation of membrane glycoproteins by NEU1 and its potential implication in receptor transactivation.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Microbiology
Tatiana M. T. Rezende, Gabriella Macera, Leo Heyndrickx, Johan Michiels, Sandra Coppens, Hendrik Jan Thibaut, Kai Dallmeier, Marjan Van Esbroeck, Johan Neyts, Kevin K. Arien, Koen Bartholomeeusen
Summary: This article reports the validation of a new reporter cell line, Hec1a-IFNB-Luc, for the inhibition studies of various flaviviruses relevant to human pathology. The cell line showed high responsiveness to all tested flaviviruses, including dengue virus serotypes. It is comparable to clinically applied assays in measuring neutralizing antibody activity and assessing the antiviral activities of small-molecule compounds.
MICROBIOLOGY SPECTRUM
(2023)
Article
Cardiac & Cardiovascular Systems
Qian-Qian Chen, Gaoxiang Ma, Jin-Feng Liu, Yuan-Yuan Cai, Jun-Yuan Zhang, Ting-Ting Wei, An Pan, Shujun Jiang, Yibei Xiao, Pingxi Xiao, Jiangping Song, Ping Li, Lei Zhang, Lian-Wen Qi
Summary: This study identifies NEU1 as a critical driver of cardiac hypertrophy and inhibition of NEU1 opens up a new field of treatment for cardiovascular diseases. Furthermore, anti-influenza drugs zanamivir and oseltamivir effectively inhibit mammalian NEU1, suggesting new indications for cardio-protection.
EUROPEAN HEART JOURNAL
(2021)
Article
Microbiology
David C. Bibby, Michael Savanovic, Jinrong Zhang, Alessandro Torelli, Rienk E. Jeeninga, Luc Gagnon, Shannon L. Harris
Summary: The study assesses the reproducibility and consistency of the hemagglutination inhibition (HI) assay in accredited laboratories, and finds that with standardization and a common protocol, the assay can provide similar results between different laboratories.
