Article
Immunology
Wei-Tong Hou, Chen-Rui Shen, Ji Peng, Li-Wen Jiang, Shi-Yu Guo, Xi-Ran Qiu, Yu Zhang, Hui Shen, Yuan-Ying Jiang, Mao-Mao An
Summary: In this study, researchers identified an antibody that could neutralize multiple toxins of Staphylococcus aureus. They screened for this all-in-one antibody using hybridoma fusion and found that it had significant pharmacodynamic effects in in vivo mouse models and in vitro experiments. The study also provided preliminary insights into the pharmacodynamics and mechanisms of this antibody.
JOURNAL OF INFECTIOUS DISEASES
(2023)
Article
Immunology
Gunjan Kak, Zachary Van Roy, Cortney E. E. Heim, Rachel W. W. Fallet, Wen Shi, Axel Roers, Bin Duan, Tammy Kielian
Summary: The study found that IL-10 derived from granulocytes plays a role in suppressing the clearance of Staphylococcus aureus during craniotomy infection, possibly by inhibiting neutrophil bactericidal activity and TNF production.
JOURNAL OF NEUROINFLAMMATION
(2023)
Article
Pharmacology & Pharmacy
Xiaoduo Li, Zhengcai Ma, Qin Tang, Zhenwei Gui, Biao Zhang, Guang Sun, Jingwei Li, Juan Li, Mengmeng Li, Xuegang Li, Hang Ma, Xiaoli Ye
Summary: This research uncovered the antimicrobial properties of a novel compound called 8-octyl berberine (OBBR) against Staphylococcus aureus. OBBR showed promising results with minimum inhibitory concentration (MIC) similar to levofloxacin, but with lower chances of inducing resistance. Mechanistically, OBBR induced morphological alterations in the bacteria, disrupted the bacterial cell wall and membrane, and inhibited the enzymatic activity of the protein MraY, ultimately inhibiting peptidoglycan synthesis. OBBR emerges as a potential alternative antibiotic against S. aureus with limited drug resistance development.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Immunology
Yanhao Zhang, Feng Yang, Dong Sun, Limin Xu, Yaojia Shi, Leilei Qin, Liqun Zhao, Liting Wang, Wei Sun, Hongri Wu, Dongshui Lu, Weijun Zhang, Ping Luo, Ping Cheng, Quanming Zou, Hao Zeng
Summary: This study reports the protective effects of a recombinant five-antigen S. aureus vaccine in an S. aureus infected bone defect model. The study found that the number of M2 macrophages increased significantly in the defect site and played a critical role in the healing process mediated by the vaccine.
CLINICAL IMMUNOLOGY
(2023)
Article
Microbiology
Linda Paetzold, Alexandra Stark, Felix Ritzmann, Carola Meier, Thomas Tschernig, Joerg Reichrath, Robert Bals, Markus Bischoff, Christoph Beisswenger
Summary: The IL-17C/IL-17RE axis plays a role in wound closure in infected wounds, but does not affect the elimination of S. aureus.
Article
Immunology
Chia-Hui Luo, Alan Chuan-Ying Lai, Ya-Jen Chang
Summary: Atopic dermatitis (AD) is an inflammatory skin disease caused by a disrupted skin barrier and dominated by type 2 immune responses. Staphylococcus aureus (S. aureus) infection poses a high risk to patients with AD. Interleukin-33 (IL-33) and butyrate have been implicated in the pathophysiology of AD, but their effects on AD aggravated by S. aureus infection are not well understood. In this study, we established an AD-like mouse model with epidermal barrier disruption and examined the effects of S. aureus and butyrate on IL-33 expression and immune response. We found that S. aureus infection exacerbated IL-33 release and skin inflammation, while butyrate attenuated S. aureus-aggravated skin inflammation through histone deacetylase 3 (HDAC3) inhibition. These findings suggest that butyrate may have a positive effect on controlling inflammatory skin conditions in AD aggravated by S. aureus infection.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Microbiology
Marina Garcia-Moreno, Paul M. Jordan, Kerstin Guenther, Therese Dau, Christian Fritzsch, Monika Vermes, Astrid Schoppa, Anita Ignatius, Britt Wildemann, Oliver Werz, Bettina Loeffler, Lorena Tuchscherr
Summary: This study investigated the interaction between Staphylococcus aureus and osteoblasts or osteocytes during infection. The results showed that osteoblasts can induce an antimicrobial response and eliminate intracellular S. aureus, while osteocytes recruit immune cells and enhance inflammation but have a poor capacity for clearing bacterial infection. Additionally, signals from osteocytes enhance intracellular bacterial clearance by osteoblasts.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Infectious Diseases
Yhu-Chering Huang, Chih-Jung Chen
Summary: The clindamycin susceptibility of MRSA in northern Taiwan significantly increased from 2008 to 2018, mainly due to the rise of USA300 strains. Whole-genome sequencing revealed that most circulating USA300 strains in Taiwan have been imported on multiple occasions.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2022)
Article
Genetics & Heredity
Anton Endutkin, Elena P. Panferova, Alexander E. Barmatov, Dmitry O. Zharkov
Summary: The study characterized the properties of putative GO system DNA glycosylases from Staphylococcus aureus, an important human opportunistic pathogen causing hospital infections. The findings suggest that S. aureus possesses an active GO system, which could possibly be targeted for sensitization of this pathogen to oxidative stress.
Article
Infectious Diseases
Mohammed S. Almuhayawi, Hattan S. Gattan, Mohammed H. Alruhaili, Mohanned Talal Alharbi, Mohammed K. Nagshabandi, Muyassar K. Tarabulsi, Saad M. Almuhayawi, Soad K. Al Jaouni, Samy Selim, Awadh Alanazi, Yasir Alruwaili, Osama Ahmed Faried, Islam Amin, Mohamed E. Elnosary
Summary: This study examined 58 patients with various types of ear infections in Sakaka, Al Jouf, Saudi Arabia and found that the most common bacteria causing ear infections were Staphylococcus aureus and Pseudomonas aeruginosa. All the bacteria strains isolated were found to have antibiotic resistance plasmids. The study concluded that vancomycin, linezolid, tigecycline, rifampin, and daptomycin are effective antibiotics against S. aureus and P. aeruginosa.
INFECTION AND DRUG RESISTANCE
(2023)
Article
Infectious Diseases
Jun Sung Hong, Dokyun Kim, Seok Hoon Jeong
Summary: This study evaluated the use of the IRBT (R) system for strain typing of S. aureus clinical isolates. The results showed that this system has the potential to rapidly discriminate strain types of S. aureus isolates.
Article
Pediatrics
Derrick Alexandre Fassbind, Raissa Queiroz Rezende, Cicero Armidio Gomes Dias, Fabrizio Motta
Summary: This study evaluated the impact of healthcare-associated and community-acquired methicillin-resistant Staphylococcus aureus (MRSA) infections in a pediatric hospital in southern Brazil. The prevalence of community-acquired MRSA infections was 46.1% and healthcare-associated MRSA infections was 8.1%, with no significant change over the study period. The study highlights the need to review initial protocols for severe staphylococcal infections based on local epidemiology.
FRONTIERS IN PEDIATRICS
(2023)
Article
Infectious Diseases
Francesc Escrihuela-Vidal, Achim J. Kaasch, Maja Von Cube, Siegbert Rieg, Winfried V. Kern, Harald Seifert, Kyoung-Ho Song, Chun-Hsing Liao, Robert Tilley, Hannah Gott, Matt Scarborough, Claire Gordon, Martin J. Llewelyn, Richard Kuehl, Laura Morata, Alex Soriano, Jonathan Edgeworth, Enrique Ruiz De Gopegui, Emmanuel Nsutebu, Jose Miguel Cisneros, Vance G. Fowler, Guy Thwaites, Joaquin Lopez-Contreras, Gavin Barlow, Hugo Guillermo Ternavasio-De La Vega, Jesus Rodriguez-Bano, Luis Eduardo Lopez-Cortes
Summary: This study analysed the adherence and impact of quality-of-care indicators (QCIs) in the management of Staphylococcus aureus bloodstream infection. The results showed that adherence to QCIs was associated with reduced 90-day mortality.
CLINICAL MICROBIOLOGY AND INFECTION
(2023)
Article
Infectious Diseases
Helle Brander Eriksen, Andreas Petersen, Michael Pedersen, Soren Overballe-Petersen, Anders Rhod Larsen, Barbara Juliane Holzknecht
Summary: This case report describes recurrent catheter-related bloodstream infections caused by Staphylococcus aureus, initially susceptible to penicillin but later developing resistance. Experimental findings showed that the loss of penicillin resistance correlated with the absence of the blaZ gene.
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
(2022)
Article
Microbiology
Chitra Subramanian, Matthew W. W. Frank, My-Kyung Yun, Charles O. Rock
Summary: Glycerol ester hydrolase (Geh) is a major secreted lipase in Staphylococcus aureus that plays a role in virulence. It is responsible for the formation and release of a15-LPG, a major membrane phospholipid derived from phosphatidylglycerol (PG). Geh hydrolyzes the 1-position acyl chain of PG to generate a15-LPG, which has important physiological roles in S. aureus membrane phospholipid turnover. The positional specificity of Geh is explained by its active site structure.
Article
Immunology
Matthew M. Schaefers, Biyan Duan, Boaz Mizrahi, Roger Lu, Gally Reznor, Daniel S. Kohane, Gregory P. Priebe
Review
Infectious Diseases
Christina Merakou, Matthew M. Schaefers, Gregory P. Priebe
SURGICAL INFECTIONS
(2018)
Article
Microbiology
Laura C. C. Cook, Nilanjana Chatterjee, Yan Li, Jorge Andrade, Michael J. Federle, Zehava Eichenbaum
Review
Immunology
Lamar Thomas, Laura Cook
INFECTION AND IMMUNITY
(2020)
Review
Immunology
Matthew M. Schaefers
INFECTION AND IMMUNITY
(2020)
Article
Microbiology
Nilanjana Chatterjee, Laura C. C. Cook, Kristin V. Lyles, Hong Anh T. Nguyen, Darius J. Devlin, Lamar S. Thomas, Zehava Eichenbaum
JOURNAL OF BACTERIOLOGY
(2020)
Editorial Material
Immunology
Laura C. Cook
Summary: The M28 Streptococcus pyogenes strains are highly associated with life-threatening puerperal infections, with genome sequencing revealing a large mobile genetic element, RD2. This element, linked to the ability of M28 GAS to colonize the vaginal tract, has a serotype preference that researchers are trying to understand through gain-of-function mutants in different GAS serotypes.
INFECTION AND IMMUNITY
(2021)
Article
Microbiology
Matthew M. Schaefers, Benjamin X. Wang, Nicole M. Boisvert, Sarah J. Martini, Sarah L. Bonney, Christopher W. Marshall, Michael T. Laub, Vaughn S. Cooper, Gregory P. Priebe
Summary: Mutations in the fixL gene of BCC play a significant role in bacterial adaptation during chronic lung infection in CF patients, enhancing survival in macrophages and the murine lung environment at the expense of reduced fitness in soil.
Article
Multidisciplinary Sciences
Hattie Chung, Christina Merakou, Matthew M. Schaefers, Kelly B. Flett, Sarah Martini, Roger Lu, Jennifer A. Blumenthal, Shanice S. Webster, Ashley R. Cross, Roy Al Ahmar, Erin Halpin, Michelle Anderson, Nicholas S. Moore, Eric C. Snesrud, Hongwei D. Yu, Joanna B. Goldberg, George A. O'Toole, Patrick McGann, Jason A. Stam, Mary Hinkle, Alexander J. McAdam, Roy Kishony, Gregory P. Priebe
Summary: The evolution of antibiotic resistance in individual patients is not well understood when rapid antibiotic switching occurs. In this study, it was found that rare antibiotic resistance mutations can emerge and expand rapidly in response to antibiotic changes, while mutations conferring resistance to antibiotics not administered can diminish and even go to extinction. Deep genomic surveillance can inform patient-specific antibiotic cycling strategies to drive resistance mutations to extinction during early stages of infection.
NATURE COMMUNICATIONS
(2022)
Article
Immunology
Kristin V. Lyles, Lamar S. Thomas, Corbett Ouellette, Laura C. C. Cook, Zehava Eichenbaum
Summary: The study demonstrates the important role of HupZ protein in heme metabolism and host survival in Group A Streptococcus (GAS). HupZ can bind and degrade heme, but it does not participate in heme iron coordination. HupZ can also bind and partially degrade a fragment of heme c. A hupZ mutant strain shows reduced growth, increased sensitivity to heme toxicity, and decreased fitness in a murine colonization model.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Immunology
Mohammad Omar Faruk Shaikh, Matthew M. Schaefers, Christina Merakou, Marco DiBlasi, Sarah Bonney, Tiffany Liao, David Zurakowski, Margaret Kehl, David E. Tabor, Antonio DiGiandomenico, Gregory P. Priebe
Summary: In this study, the combination vaccine of PopB and OprF/I for P. aeruginosa was evaluated. The results showed that the combination vaccine induced a strong immune response and enhanced protection against acute lethal P. aeruginosa pneumonia.
INFECTION AND IMMUNITY
(2022)
Article
Microbiology
Lamar S. Thomas, Laura C. Cook
Summary: Neonatal GBS disease is a major health concern associated with maternal vaginal colonization. Vaccine development has been challenging, but the addition of a protein conjugate may enhance immunogenicity and coverage across GBS serotypes.
Article
Immunology
Lamar S. Thomas, Nicholas A. Faiola, Emily Canessa, Yetrib Hathout, Laura C. Cook
Summary: This study evaluates the prevalence and relevance of BvaP repeated domains in GBS vaginal colonization. In silico analysis reveals that the number of repeats in the BvaP protein is not associated with GBS serotype, isolation site, or host. Experimental findings suggest that a smaller number of repeats leads to decreased bacterial chain length, but adherence to vaginal epithelial cells can be complemented using BvaP with different numbers of repeats. Further research will focus on understanding the host immune response to BvaP in vivo and the dependence of vaginal carriage or host response on the BvaP repeated domains.
INFECTION AND IMMUNITY
(2023)
Article
Chemistry, Multidisciplinary
Mehdi Tahernia, Ellie Plotkin-Kaye, Maedeh Mohammadifar, Yang Gao, Melissa R. Oefelein, Laura C. Cook, Seokheun Choi
Meeting Abstract
Pediatrics
S. Bonney, L. J. Caverly, G. Priebe, M. M. Schaefers
PEDIATRIC PULMONOLOGY
(2020)