Article
Energy & Fuels
Eliane V. Barros, Lindamara M. Souza, Nata C. L. Madeira, Luiz S. Chinelatto, Juliana N. Bertelli, Rogerio M. Carvalho, Boniek G. Vaz, Rosineide C. Simas, Alvaro C. Neto, Valdemar Lacerda, Wanderson Romao
Summary: This study presents an alternative method for the specific isolation of ARN from calcium naphthenate deposits, by cleaning and converting the salts, successfully separating the ARN species, supported by high-resolution mass spectrometry techniques.
Article
Energy & Fuels
Abdul Gani Abdul Jameel, Awad B. S. Alquaity, Felipe Campuzano, Abdul-Hamid Emwas, Saumitra Saxena, S. Mani Sarathy, William L. Roberts
Summary: VR is a byproduct of crude oil vacuum distillation, primarily used for the production of syn-gas, hydrogen, heavy fuel oil for power generation and shipping. Due to its high sulfur content, desulfurization is necessary according to emission standards. Detailed molecular characterization of sulfur species in VR can be achieved using high-resolution FT-ICR MS, enabling classification and understanding of the sulfur structures present.
Article
Biochemistry & Molecular Biology
Anallely Lopez-Yerena, Anna Vallverdu-Queralt, Olga Jauregui, Xavier Garcia-Sala, Rosa M. Lamuela-Raventos, Elvira Escribano-Ferrer
Summary: This study investigated the distribution of oleocanthal and its metabolites in rat plasma and tissues after the ingestion of refined olive oil. Oleocanthal was mainly detected in the stomach and intestine samples, with concentrations decreasing over time. Ten metabolites were identified, with the small intestine and liver serving as the most important metabolizing organs. The main circulating metabolites were identified as OLC + OH + CH3 and OLC + H2O + glucuronic acid, which may contribute to the health benefits associated with the regular consumption of extra virgin olive oil. Additional research is needed to determine the concentrations and molecular structures of these metabolites in human plasma and tissues.
Article
Energy & Fuels
Fernanda E. Pinto, Victor R. Fonseca, Lindamara M. Souza, Luciana A. Terra, Sreedhar Subramanian, Sebastien Simon, Johan Sjoblom, Thieres M. Pereira, Valdemar Lacerda, Wanderson Romao
Summary: Negative ion-mode electrospray coupled with Fourier transform ion cyclotron resonance mass spectrometry allows for high resolution and accurate ionization of polar constituents. This study characterized different asphaltene subfractions obtained through precipitation and adsorption, estimating solubility parameters and revealing a higher number of polar species in certain subfractions with a more aromatic profile. The study also found that species containing multiple heteroatoms showed higher solubility parameters, impacting the solubility of subfractions.
Article
Chemistry, Analytical
Qiong Pan, Wenya Hu, Ding He, Chen He, Linzhou Zhang, Quan Shi
Summary: High-resolution mass spectrometry provides compositional information of dissolved organic matter through isotopic assignment, but multiple possible solutions often occur due to measurement deviation and resolving power limitation. To improve result accuracy in an automated manner, a machine-learning-based algorithm was developed, which showed a significant improvement in formula assignment compared to traditional methods.
Article
Geochemistry & Geophysics
Bin Jiang, Yankuan Tian, Zheng Zhai, Zhao-Wen Zhan, Yuhong Liao, Yan-Rong Zou, Ping'an Peng
Summary: The study found that heteroatomic compounds in bound bitumen are better proxies of the original biogenic sources and contain more aromatic structures than in heteroatomic compounds occurring in the free bitumen. Differences in the maturity, depositional environment, and diagenetic conditions significantly influenced the composition and distribution of heteroatomic compounds in free and bound bitumen from different source rocks.
ORGANIC GEOCHEMISTRY
(2021)
Article
Food Science & Technology
Daria Kucheriavaia, Dusan Velickovic, Nicholas Peraino, Apurva Lad, David J. Kennedy, Steven T. Haller, Judy A. Westrick, Dragan Isailovic
Summary: This study investigated the distribution of MC-LR in liver tissues of mice using MALDI-FT-ICR-MS imaging techniques. The results show that high-resolution MALDI-MS imaging can monitor the distribution of free microcystins in affected organs.
Article
Meteorology & Atmospheric Sciences
Yaling Zeng, Yanli Ning, Zhenxing Shen, Leiming Zhang, Tian Zhang, Yali Lei, Qian Zhang, Guohui Li, Hongmei Xu, Steven Sai Hang Ho, Junji Cao
Summary: Brown Carbon (BrC) molecules are known for absorbing light in specific wavelengths, with nitrogen-containing organic molecules identified as critical components of light-absorbing matters in both winter and summer seasons. The top BrC molecules were found to be responsible for the majority of absorbances in both seasons, with different relationships between nitrogen and sulfur compounds identified in winter and summer processes.
JOURNAL OF GEOPHYSICAL RESEARCH-ATMOSPHERES
(2021)
Article
Agricultural Engineering
Konstantin Moiseenko, Olga A. Glazunova, Olga S. Savinova, Daria V. Vasina, Alexander Ya Zherebker, Natalia A. Kulikova, Evgeny N. Nikolaev, Tatiana Fedorova
Summary: The study found that during the modification of kraft lignin by Trametes hirsuta fungus, the fungus tended to degrade more reduced compounds and produce more oxidized compounds as cultivation progressed. The secretion of ligninolytic peroxidases by the fungus changed in a cascade manner, with the later secreted peroxidases showing higher substrate affinity for phenolic compounds and acting in a more specialized manner compared to the early secreted ones.
BIORESOURCE TECHNOLOGY
(2021)
Article
Green & Sustainable Science & Technology
Dongdong Ge, Shouqiang Huang, Jiehong Cheng, Ying Han, Yuhui Wang, Yanting Dong, Jinwen Hu, Guobiao Li, Haiping Yuan, Nanwen Zhu
Summary: The PFS/O-3 process enhances sludge dewaterability through ozonation and polyferric sulfate catalysis, while also removing heavy metals and polycyclic aromatic hydrocarbons. It shows great potential and practical value in sludge treatment.
JOURNAL OF CLEANER PRODUCTION
(2022)
Article
Energy & Fuels
Hanjing Zhang, Sumei Li
Summary: This study characterized saline lacustrine immature oils and freshwater lacustrine mature oils using positive-ion electrospray ionization FT-ICR MS. The results showed a genetic relationship between organosulfur compounds (OSCs) and their precursors, and certain ratios were found to be sensitive indicators of organofacies. Newly developed maturity indices accurately assessed the thermal maturity of lower maturity oils.
Article
Biochemical Research Methods
Jihyeon Lee, Min Sun Kim, Hyun Sik Kim, Yoong-Kee Choe, Soo Gyeong Cho, Eun Mee Goh, Jeongkwon Kim
Summary: This study comprehensively characterized the adduct ions of RDX and HMX using ultrahigh resolution and accuracy 15-T FT-ICR MS, and discovered new adduct ions. Different types of adduct ions were observed using different ionization sources. The identification of the [M + NO2](-) adduct ion in the ESI-MS analysis of RDX and HMX is a novel finding reported for the first time.
JOURNAL OF MASS SPECTROMETRY
(2021)
Article
Geochemistry & Geophysics
Yankuan Tian, Bin Jiang, Jian Chen, Zhao-Wen Zhan, Chao Yang, Yan-Rong Zou, Ping'an Peng
Summary: This study investigated the released heteroatomic compounds from asphaltene-bound fractions of crude oils and their compositions and distributions provide insights into the origins and processes of hydrocarbon accumulation.
ORGANIC GEOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Steffen Lippold, Steffen Lippold, Raashina Thavarajah, Raashina Thavarajah, Dietmar Reusch, Dietmar Reusch, Manfred Wuhrer, Manfred Wuhrer, Simone Nicolardi, Simone Nicolardi
Summary: Recombinant human erythropoietin (EPO) is a complex therapeutic glycoprotein with several glycosylation sites, and the analysis of EPO glycosylation is crucial for its safety and efficacy. Various mass spectrometry methods are used to study EPO glycosylation, with ESI MS providing comprehensive insights into intact EPO glycoform heterogeneity.
ANALYTICA CHIMICA ACTA
(2021)
Article
Energy & Fuels
Chen He, Wei-Ming Chen, Chun-Mao Chen, Quan Shi
Summary: In this study, mass spectrometry was used to analyze the molecular composition of dissolved organic matter (DOM) in refinery wastewater during the treatment process. Different ionization modes were employed to characterize the DOM molecules, and significant differences in heteroatom composition, DOM constituents, and chemical properties were observed. The transformation characteristics and mechanisms of various organic compounds in refinery wastewater were determined, providing guidance for the design and optimization of wastewater treatment technologies.
Article
Oncology
Volundur Hafstao, Rolf Sokilde, Jari Hakkinen, Malin Larsson, Johan Vallon-Christersson, Carlos Rovira, Helena Persson
Summary: Research has found that fusion transcripts involving miRNA host genes in breast tumors are non-random, with overrepresentation of highly expressed genes in cancer pathways. Additionally, many miRNAs were upregulated in samples with host gene fusions, including established onco-genic miRNAs. This suggests that miRNA host fusions may act as "driver" mutations in tumors.
INTERNATIONAL JOURNAL OF CANCER
(2022)
Article
Urology & Nephrology
Sigrid Carlsson, Rebecka Arnsrud Godtman, Carl-Gustav Pihl, Andrew Vickers, Hans Lilja, Jonas Hugosson, Marianne Mansson
Summary: This study aimed to investigate the relationship between the age at first prostate-specific antigen (PSA) screening and the reduction in prostate cancer mortality risk. The results showed that starting screening at a younger age was associated with a greater reduction in prostate cancer mortality risk. It is recommended that PSA screening should start no later than age 55.
Correction
Urology & Nephrology
Sigrid Carlsson, Rebecka Arnsrud Godtman, Carl-Gustav Pihl, Andrew Vickers, Hans Lilja, Jonas Hugosson, Marianne Mansson
Article
Medicine, Research & Experimental
Friederike Praus, Axel Kuenstner, Thorben Sauer, Michael Kohl, Katharina Kern, Steffen Deichmann, Akos Vegvari, Tobias Keck, Hauke Busch, Jens K. K. Habermann, Timo Gemoll
Summary: This study integrated genomic, transcriptomic, and proteomic analyses to identify significant differences in expression during colorectal cancer progression. The results highlight the importance of tumor heterogeneity and individual differences for clinical studies, and may accelerate the implementation of precision oncology in the future.
JOURNAL OF TRANSLATIONAL MEDICINE
(2023)
Article
Infectious Diseases
Rafael Cena-Diez, Aswathy Narayanan, Shilpa Ray, Maarten van de Klundert, Jimmy E. Rodriguez, Johan Nilvebrant, Per-Ake Nygren, Akos Vegvari, Robert van Domselaar, Anders Sonnerborg
Summary: WC-am, a dipeptide, has been found to be increased in elite controllers, who can control HIV-1 infection. This study aimed to evaluate the anti-HIV-1 activity and mechanism of action of WC-am. The results showed that WC-am binds to the CD4 binding pocket of HIV-1 gp120, blocking its binding to host cell receptors, and inhibits HIV-1 replication by affecting reverse transcription.
INTERNATIONAL JOURNAL OF ANTIMICROBIAL AGENTS
(2023)
Article
Immunology
Yu-Ching Su, Mahendar Kadari, Megan L. L. Straw, Martina Janouskova, Sandra Jonsson, Oskar Thofte, Farshid Jalalvand, Erika Matuschek, Linda Sandblad, Akos Vegvari, Roman A. A. Zubarev, Kristian Riesbeck
Summary: Non-typeable Haemophilus influenzae (NTHi) is a Gram-negative human pathogen that causes various airway diseases. The outer membrane protein P5 contributes to bacterial serum resistance and also plays a critical role in maintaining bacterial outer membrane integrity and protein composition.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2023)
Article
Immunology
Abdul Muktadir Shafi, Akos Vegvari, Roman A. Zubarev, Carlos Penha-Goncalves
Summary: This study reveals the important role of the STING-INFb-CXCL10 pathway in brain microvascular endothelial cells (BECs) in the development of cerebral malaria (CM). Activation of this pathway leads to immunoproteasome activation, enhanced antigen presentation, and impairment of endothelial barrier function. These findings provide insights into the pathogenesis of CM and its lethality.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Medicine, Research & Experimental
Huqiao Luo, Vilma Urbonaviciute, Amir Ata Saei, Hezheng Lyu, Massimiliano Gaetani, Akos Vegvari, Yanpeng Li, Roman A. Zubarev, Rikard Holmdahl
Summary: Mutations in NCF1 gene that cause low production of ROS are strongly linked to systemic lupus erythematosus. The deficiency of ROS in plasmacytoid dendritic cells (pDCs) exacerbates lupus development by promoting pDC accumulation in multiple organs, elevating IFN-alpha levels, and activating IFN-stimulated genes. Mechanistic studies show that ROS deficiency enhances pDC generation and migration, which further augment type I IFN responses. Restoring NOX2-derived ROS specifically in pDCs protects against lupus by suppressing these pathways.
Article
Engineering, Biomedical
Milan Finn Wesseler, Nayere Taebnia, Sean Harrison, Sonia Youhanna, Lena C. Preiss, Aurino M. Kemas, Akos Vegvari, Jaroslav Mokry, Gareth J. Sullivan, Volker M. Lauschke, Niels B. Larsen
Summary: This study presents an accurate liver microphysiological system (MPS) created through engineering of 3D printed hydrogel chips with synthetic vasculature channels. The MPS allows the long-term culture of human liver cells and demonstrates improved molecular phenotypes compared to other 3D culture methods. The platform also successfully generates physiologically relevant oxygen gradients and shows zonation-specific toxicity patterns.
ACTA BIOMATERIALIA
(2023)
Article
Biochemical Research Methods
Voelundur Hafstao, Jari Hakkinen, Helena Persson
Summary: This article presents a method for validating fusion transcripts detected by RNA sequencing in matched whole-genome sequencing data. The pipeline uses discordant read pairs and soft-clipped read alignments to identify supported fusion events and determine genomic breakpoints. The method is faster and more sensitive than commonly used structural variant detection software BreakDancer and Manta.
BMC BIOINFORMATICS
(2023)
Article
Biochemistry & Molecular Biology
Christian M. Beusch, Oscar E. Simonson, Johan O. Wedin, Pierre Sabatier, Ulrika Felldin, Sandeep Kadekar, Cecilia Osterholm, Akos Vegvari, Roman A. Zubarev, Karin Fromell, Bo Nilson, Stefan James, Elisabeth Stahle, Karl-Henrik Grinnemo, Sergey Rodin
Summary: By comparing the extracellular matrix proteins in valve tissues from patients with isolated aortic valve degeneration, the study identified differences in the molecular cues and aetiologies between patients with tricuspid and bicuspid aortic valves, suggesting the need for different treatments and providing insights into the molecular basis of the condition.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Article
Dentistry, Oral Surgery & Medicine
Alfredo Torres, M. Angelica Michea, Akos Vegvari, Marion Arce, Alicia Morales, Elias Lanyon, Marcela Alcota, Camila Fuentes, Rolando Vernal, Mauricio Budini, Roman A. Zubarev, Fermin E. Gonzalez
Summary: The study aimed to explore the qualitative and quantitative differences in gingival crevicular fluid (GCF) proteome of periodontitis patients during disease progression. It was found that the GCF proteome of progressive periodontitis was associated with metabolic processes, immune response, and cellular stress, while the proteome of stable periodontitis was mainly related to wound repair, cell death regulation, and autophagy.
JOURNAL OF PERIODONTAL RESEARCH
(2023)
Article
Biochemical Research Methods
Akos Vegvari, Xuepei Zhang, Roman A. Zubarev
Summary: This study successfully detected proteins from individual Escherichia coli bacteria for the first time, with validation of the findings through comparison with other samples and bulk proteomics data.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2023)
Article
Neurosciences
Robert D. Hawkins, Lennart Brodin, Elvar Theodorsson, Akos Vegvari, Eric R. Kandel, Tomas Hokfelt
Summary: Neuropeptides are widely used as neurotransmitters in both vertebrates and invertebrates, but the complexity of the vertebrate nervous system has hindered the understanding of their functions as transmitters. The marine mollusk Aplysia, with its simpler nervous system and large identified neurons, offers advantages for studying neuropeptides' roles in behavior. By observing immunoreactivity in individual neurons in adult Aplysia, we have discovered potential unknown peptides involved in behavior. Furthermore, our studies have revealed a high degree of colocalization of different neuropeptides in individual neurons, suggesting complex interactions and functions.
Article
Biotechnology & Applied Microbiology
Volundur Hafstao, Jari Hakkinen, Malin Larsson, Johan Staaf, Johan Vallon-Christersson, Helena Persson
Summary: A process for validating fusion transcripts and a machine learning classifier were developed to improve identification accuracy, extending the detection of potential targetable kinase fusions. Machine learning can be utilized for clinically relevant fusion events identification for targeted therapy, with the generated dataset serving as a valuable resource for fusion transcript detection algorithm development and evaluation.
