Review
Cardiac & Cardiovascular Systems
Camilla Paoletti, Valeria Chiono
Summary: Ischemic heart disease is a major cause of mortality worldwide, and current pharmacological approaches have not yet achieved cardiac regeneration. Direct reprogramming of fibroblasts into induced cardiomyocytes and the use of microRNAs for transfection show promising potential for myocardial regeneration. Further advancements in nanocarrier technology and 3D culture microenvironments are enhancing the efficiency of this approach for future clinical translation.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Review
Biochemistry & Molecular Biology
Yevgeniy Kim, Sanzhar Nurakhayev, Ayan Nurkesh, Zharylkasyn Zharkinbekov, Arman Saparov
Summary: Cardiovascular disease is the leading cause of mortality and morbidity globally, with myocardial infarction playing a significant role in its detrimental impact. Macrophages are important regulators in the inflammation and fibrosis following myocardial infarction, and therapeutic strategies should focus on utilizing their polarization towards anti-inflammatory or reparative phenotypes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Review
Medicine, Research & Experimental
Xuerui Chen, Liyun Zhu, Xu Wang, Junjie Xiao
Summary: This article introduces the application of hydrogels in cardiac repair following myocardial infarction (MI). Hydrogels with heart-specific mimicry and extracellular matrix (ECM)-like functionality can mechanically constrain the infarct and act as localized transport of bioactive ingredients to refresh the dysfunctional heart. The article also summarizes the advantages of heart-tailored architectures of hydrogels in cardiac repair and proposes challenges and prospects in the clinical translation of hydrogels for cardiac repair and regeneration after MI.
MOLECULAR PHARMACEUTICS
(2023)
Article
Oncology
Nikolaos Bekas, Martina Samiotaki, Maria Papathanasiou, Panagiotis Mokos, Athanasios Pseftogas, Konstantinos Xanthopoulos, Dimitris Thanos, George Mosialos, Dimitra Dafou
Summary: This study reveals the important regulatory role of CYLD in the early stages of somatic cell reprogramming. CYLD DUB deficiency reduces reprogramming efficiency and hinders the mesenchymal-to-epithelial transition (MET) process. These findings expand our understanding of early reprogramming mechanics and highlight the significance of CYLD as an extracellular matrix regulator.
Article
Chemistry, Multidisciplinary
Jayachandra Reddy Nakkala, Yuejun Yao, Zihe Zhai, Yiyuan Duan, Deteng Zhang, Zhengwei Mao, Linrong Lu, Changyou Gao
Summary: The itaconate derivative DMI can modulate cell metabolism by regulating inflammatory responses and promoting antioxidant reactions, thus enabling proper tissue repair.
Article
Cell Biology
Ah Reum Lee, Ji-Hoon Park, Sung Han Shim, Kwonho Hong, Hyeonwoo La, Kyung-Soon Park, Dong Ryul Lee
Summary: The study found that the activity of Rad 51 is lower in SCNT eggs and a significantly lower level of DSBs in SCNT embryos during reprogramming. Supplementation with RS-1 during the activation of SCNT embryos can increase RAD51 expression and DSB foci, thereby improving the efficiency of SCNT reprogramming. Through single-cell RNA-seq analysis, reactivation of genes and involvement of histone demethylase in reducing reprogramming-resistant regions were observed, which may contribute to overcoming developmental block and enhancing cell sources for regenerative medicine.
CELL PROLIFERATION
(2021)
Article
Multidisciplinary Sciences
Shingo Narita, Kazumasa Unno, Katsuhiro Kato, Yusuke Okuno, Yoshitaka Sato, Yusuke Tsumura, Yusuke Fujikawa, Yuuki Shimizu, Ryo Hayashida, Kazuhisa Kondo, Rei Shibata, Toyoaki Murohara
Summary: This study demonstrated the successful direct reprogramming of adult ADRCs into cardiomyocytes, and showed the therapeutic potential of these reprogrammed cells in treating acute myocardial infarction.
Review
Cell Biology
Xingyu He, Jialiang Liang, Christian Paul, Wei Huang, Suchandrima Dutta, Yigang Wang
Summary: Continuous loss of cardiomyocytes is a fundamental characteristic of heart diseases, limiting the efficacy of treatments. Recent research has shown promising therapeutic methods for regenerative heart repair through cell reprogramming and direct cell lineage conversion. Studies on cellular and molecular pathways have also revealed essential mechanisms and potential applications of genomic or epigenetic editing in cellular reprogramming.
Review
Cell Biology
Young-Jae Nam
Summary: Loss of cardiac muscle after injury is replaced by fibrosis, and directly reprogramming fibroblasts into induced cardiomyocyte-like cells is an attractive regenerative approach. However, its translation into clinical practice still faces challenges.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Naixin Wang, Weiwei Wang, Xiaoqi Wang, Ge Mang, Jianfeng Chen, Xiangyu Yan, Zhonghua Tong, Qiannan Yang, Mengdi Wang, Liangqi Chen, Ping Sun, Yupeng Yang, Jingxuan Cui, Mian Yang, Yafei Zhang, Dongni Wang, Jian Wu, Maomao Zhang, Bo Yu
Summary: Histone lactylation promotes early remote activation of reparative transcriptional response in monocytes, which is essential for establishing immune homeostasis and timely activation of the cardiac repair process post-MI.
CIRCULATION RESEARCH
(2022)
Article
Engineering, Biomedical
Junnan Tang, Xiaolin Cui, Zenglei Zhang, Yanyan Xu, Jiacheng Guo, Bram G. Soliman, Yongzheng Lu, Zhen Qin, Qiguang Wang, Hu Zhang, Khoon S. Lim, Tim B. F. Woodfield, Jinying Zhang
Summary: This study proposes a non-injection method for delivering EVs onto the heart surface to treat MI by spraying a mixture of EVs, GelMA precursors, and photoinitiators. The physically entrapped EVs within the GelMA hydrogel network have an enhanced retention rate and are gradually released through diffusion and/or enzymatic degradation to exert a therapeutic effect in the heart tissue.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Cell Biology
Yifang Xie, Jiandong Liu, Li Qian
Summary: The adult human heart has limited regenerative capacity. Direct cardiac reprogramming, which converts cardiac fibroblast into functional cardiomyocyte-like cells, holds great promise for heart regeneration. Significant progress has been made in improving reprogramming efficiency and understanding the underlying molecular mechanisms.
SEMINARS IN CELL & DEVELOPMENTAL BIOLOGY
(2022)
Review
Oncology
Yanjie Guo, Weini Wu, Xueyi Yang, Xiaobing Fu
Summary: Cell dedifferentiation or reprogramming is a crucial process for animals to deal with injury and promote endogenous tissue repair. Chronic damage may lead to cell dedifferentiation or reprogramming and subsequently develop into cancer. Understanding the mechanisms of cell dedifferentiation or reprogramming has significant implications for wound repair, cancer treatment, and prevention.
MOLECULAR MEDICINE REPORTS
(2022)
Review
Cardiac & Cardiovascular Systems
Alessandra Maria Lodrini, Marie-Jose Goumans
Summary: Despite the advancement in interventional coronary reperfusion strategies, mortality from acute myocardial infarction remains high. MI leads to adverse remodelling in the affected myocardium, with cellular phenotypes like senescence, inflammation, and dedifferentiation playing key roles in the pathophysiology of the infarcted heart. Enhancing electric coupling between cardiomyocytes, eliminating senescent cells, and upregulating cellular processes like autophagy could potentially improve cardiac contractility post-MI.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Marie Gunthel, Karel van Duijvenboden, Dennis E. M. de Bakker, Ingeborg B. Hooijkaas, Jeroen Bakkers, Phil Barnett, Vincent M. Christoffels
Summary: Myocardial infarction leads to changes in gene expression and epigenetic states of border zone cardiomyocytes, which may play a crucial role in maintaining the metabolic switch in these cells.
JOURNAL OF CARDIOVASCULAR DEVELOPMENT AND DISEASE
(2021)
Article
Cell & Tissue Engineering
Grace L. Johnson, Timothy D. Henry, Thomas J. Povsic, Douglas W. Losordo, Ross F. Garberich, Larissa Stanberry, Craig E. Strauss, Jay H. Traverse
STEM CELLS TRANSLATIONAL MEDICINE
(2020)
Article
Medicine, Research & Experimental
Chengming Fan, Yasin Oduk, Meng Zhao, Xi Lou, Yawen Tang, Danielle Pretorius, Mani T. Valarmathi, Gregory P. Walcott, Jinfu Yang, Philippe Menasche, Prasanna Krishnamurthy, Wuqiang Zhu, Jianyi Zhang
Review
Cell Biology
Mallikarjun Patil, Sarojini Singh, John Henderson, Prasanna Krishnamurthy
Summary: COVID-19, caused by SARS-CoV-2, has become a global pandemic leading to severe health consequences, particularly cardiac injuries, with limited understanding of its pathogenesis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2021)
Article
Cell Biology
Ling Gao, Lu Wang, Yuhua Wei, Prasanna Krishnamurthy, Gregory P. Walcott, Philippe Menasche, Jianyi Zhang
SCIENCE TRANSLATIONAL MEDICINE
(2020)
Review
Cell Biology
Jayavardini Vasanthan, Narasimman Gurusamy, Sheeja Rajasingh, Vinoth Sigamani, Shivaani Kirankumar, Edwin L. Thomas, Johnson Rajasingh
Summary: Mesenchymal stem cells (MSCs) are multipotent cells with the potential for tissue regeneration, can be easily isolated and cultured in vitro, and have been proven to be valuable for therapy. Quality control parameters, mechanisms of action, and applications in regenerative medicine and tissue engineering are important aspects to consider when utilizing MSCs.
Article
Oncology
Narasimman Gurusamy, Sheeja Rajasingh, Vinoth Sigamani, Reshma Rajasingh, Dona Greta Isai, Andras Czirok, Douglas Bittel, Johnson Rajasingh
Summary: Noonan syndrome (NS) is a dominant autosomal genetic disorder associated with mutations in various genes, including cardiac defects. Reprogramming cardiac fibroblasts from NS patients into induced pluripotent stem cells (iPSCs) revealed a heterozygous single nucleotide variation in the SOS1 gene. Further differentiation of these iPSCs into induced cardiomyocytes showed significantly reduced cardiac genes expression, indicating potential for modeling NS pathologic mechanisms and developing novel drugs for cardiomyopathy.
EXPERIMENTAL CELL RESEARCH
(2021)
Article
Cardiac & Cardiovascular Systems
Hien Luong, Sarojini Singh, Mallikarjun Patil, Prasanna Krishnamurthy
Summary: This study investigated cardiac structural and functional alterations as well as changes in myocardial glycosaminoglycan profile in mice exhibiting depression-like behavior. It found that depression in mice was associated with decreased BDNF protein expression, increased IL-6 mRNA expression, and structural changes in the heart. The study highlights the need for further research on the relationship between cardiac GAGs biology and myocardial remodeling underlying cardiovascular complications in patients with MDD.
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
(2021)
Article
Cardiac & Cardiovascular Systems
Prabhat Ranjan, Rajesh Kumari, Sumanta Kumar Goswami, Jing Li, Harish Pal, Zainab Suleiman, Zhongjian Cheng, Prasanna Krishnamurthy, Raj Kishore, Suresh Kumar Verma
Summary: This study highlights the crucial role of miR-200a-3p enriched in activated fibroblast-derived exosomes in regulating endothelial cell biology and function.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
John Henderson, Praveen K. Dubey, Mallikarjun Patil, Sarojini Singh, Shubham Dubey, Rajasekaran Namakkal Soorappan, Ramaswamy Kannappan, Palaniappan Sethu, Gangjian Qin, Jianyi Zhang, Prasanna Krishnamurthy
Summary: The study revealed that miR-377 plays a significant role in DOX-induced cardiomyocyte cell death. Inhibition of miR-377 in cell lines can protect against DOX-induced cell death and oxidative stress. However, in vivo administration of AAV9-anti-miR-377 leads to accelerated mortality and enhanced myocardial remodeling in DOX-treated mice.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Mallikarjun Patil, Sherin Saheera, Praveen K. Dubey, Asher Kahn-Krell, Prem Kumar Govindappa, Sarojini Singh, Sultan Tousif, Qinkun Zhang, Hind Lal, Jianyi Zhang, Gangjian Qin, Prasanna Krishnamurthy
Summary: The study demonstrates that mesenchymal stem cell-derived exosomes enhance macrophage engulfment of apoptotic cardiac cells, leading to improved cardiac repair and function after injury.
CIRCULATION RESEARCH
(2021)
Article
Cell Biology
Sarojini Singh, John Henderson, Mallikarjun Patil, Praveen K. Dubey, Shubham Dubey, Ramaswamy Kannappan, Jianyi Zhang, Prasanna Krishnamurthy
Summary: The study revealed that miR-181c-5p plays a crucial role in macrophage phagocytosis, potentially regulating cytoskeletal arrangement to improve the efficiency of cell ingestion. Targeting macrophage miR-181c-5p in injured tissues could lead to better clearance of dead cells and more effective resolution of inflammation.
INFLAMMATION RESEARCH
(2022)
Article
Cell Biology
Shubham Dubey, Praveen K. Dubey, Channakeshava S. Umeshappa, Yohannes T. Ghebre, Prasanna Krishnamurthy
Summary: Pathological fibrosis contributes to the progression of various diseases. This study identified the role of the RUNX1 gene in idiopathic pulmonary fibrosis (IPF) and demonstrated that increased expression of RUNX1 promotes the differentiation of fibroblasts into collagen-synthesizing myofibroblasts, leading to lung fibrosis. The study suggests that targeting RUNX1 could potentially limit the progression of organ fibrosis.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Cell Biology
Praveen K. Dubey, Mallikarjun Patil, Sarojini Singh, Shubham Dubey, Paras Ahuja, Suresh Kumar Verma, Prasanna Krishnamurthy
Summary: Endotoxemia-induced m6A methylation in the myocardium is associated with increased expression of inflammatory cytokine genes and reduced cardiac function, suggesting a potential role of m6A methylation in regulating myocardial inflammation and dysfunction during endotoxemia.
MOLECULAR AND CELLULAR BIOCHEMISTRY
(2022)
Article
Cell Biology
Mona Agrawal, Pratheepa Kumari Rasiah, Amandeep Bajwa, Johnson Rajasingh, Rajashekhar Gangaraju
Summary: This study demonstrates that induced MSCs can transfer mitochondria to immune cells to suppress effector T cells and enhance differentiation into regulatory T cells, leading to a decrease in inflammation and reduction in ischemia-reperfusion injury-induced retinal degeneration in vivo.
Review
Cell Biology
Talan Tran, Claudia Cruz, Anthony Chan, Salma Awad, Johnson Rajasingh, Richard Deth, Narasimman Gurusamy
Summary: This review focuses on the importance and potential roles of MSC-derived lncRNAs in cardiac regeneration. Understanding the molecular mechanisms of lncRNAs involved in cardiac repair and regeneration may contribute to the development of novel therapeutic strategies for cardiovascular diseases.
