Efficiency of γ-oryzanol against the complex Fusarium graminearum growth and mycotoxins production

The ability of gamma-oryzanol against Fusarium graminearum growth and mycotoxins production were assessed and compared with the antioxidant properties in order to improve its application in food chain, beyond of the conventional. The gamma-oryzanol extracted from rice bran and characterized by HPLC-UV and GG-FD. Its antifungal in vitro, effect on complex F. graminearum CQ244 (halo diameter, ergosterol and glucosamine production) and mycotoxin production (deoxinivalenol, nivalenol and zearalenone) were determined by an adapted method adapted. The antifungal activity of gamma-oryzanol was compared with azoxystrobin and trifloxystrobin. MIC and specific inhibitory effects (%inhibition mu g gamma-oryzanol(-1)) were also estimated. The antioxidant activity of the gamma-oryzanol (DPPH, FRAP, ABTS and enzymatic inhibition) was compared with the gallic acid. Considering the MIC (1, 2 mg.g(-1)) specific inhibitory effects on fungal growth (0.045%.mu g) and mycotoxin (DON 0.001%. mu g and NIV 0.002%. ug). Therefore gamma-oryzanol is also a potential natural antifungal that is able to difficult the synthesis of fungal cell wall.