4.5 Article

A New and Sensitive Catalytic Resonance Scattering Spectral Assay for the Detection of Laccase Activity Using H2O2-I--TDMAC System

Journal

CHINESE JOURNAL OF CHEMISTRY
Volume 29, Issue 4, Pages 787-792

Publisher

WILEY-V C H VERLAG GMBH
DOI: 10.1002/cjoc.201190159

Keywords

laccase; tetradecyl dimethylbenzyl ammonium chloride (TDMAC); association complex particle; catalytic resonance scattering assay

Funding

  1. National Natural Science Foundation of China [20865002, 20965002]
  2. Guangxi Normal University
  3. Ministry of Education [10012018]

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In pH 3.8 acetic acid-sodium acetate (HAC-NaAC) buffer solution, laccase exhibited a strong catalytic effect on the H2O2 oxidation of I- to form I-2, and I-2 combined with excess I- to form I-3 that reacted with cationic surfactants of tetradecyl dimethylbenzyl ammonium chloride (TDMAC) to produce the (TDMAC-I-3)(n) association complex particles, which exhibited a strong resonance scattering (RS) peak at 468 nm. Under the chosen conditions, as the concentration of laccase activity increased, the RS intensity at 468 nm (I-468 (nm)) increased linearly. The increased RS intensity Delta I-468 nm was linear to laccase activity in the range of 0.08-0.96 U/mL, with a regression equation of Delta I-468 (nm) = 88.8U-1.9, and a detection limit of 0.02 U/mL laccase. This proposed method was applied to detect laccase activity in waste water, with satisfactory results.

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