Article
Chemistry, Organic
Jiali Wang, Wubin Yao, Dandan Hu, Xinxin Qi, Jun-Qi Zhang, Hongjun Ren
Summary: A regioselective hydroboration/hydrolysis of epoxides with pinacolborane catalyzed by NaOH/BEt3 was achieved, providing secondary alcohols with exclusive selectivity and good to excellent yields. The stereochemistry of epoxides was well retained, and the corresponding secondary alcohols exhibited high optical activity under alkaline catalysis.
EUROPEAN JOURNAL OF ORGANIC CHEMISTRY
(2022)
Article
Chemistry, Inorganic & Nuclear
Gurunath Sahu, Sushree Aradhana Patra, Pratikshya Das Pattanayak, Werner Kaminsky, Rupam Dinda
Summary: In this study, four new oxidovanadium complexes were synthesized and characterized. These complexes showed interactions with DNA and BSA, and exhibited anticancer activity. Among them, complex 2 showed the highest anticancer activity against HT-29 cell line.
INORGANIC CHEMISTRY
(2023)
Article
Chemistry, Organic
Banothu Rammurthy, Swamy Peraka, Amrutham Vasu, Gajula Krishna Sai, Yennamaneni Divya Rohini, Nama Narender
Summary: A novel metal-free catalytic one-pot approach has been developed for the esterification of aralkyl ketones with alcohols under open-air atmospheric conditions. The method allows for efficient and selective synthesis of alkyl benzoates, demonstrating promising potential for scale-up applications.
ASIAN JOURNAL OF ORGANIC CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Shantanu Sen, Prerana Singh, Narendra Kumar Mishra, Subramaniam Ganesh, Sri Sivakumar, Sandeep Verma
Summary: Repeated insulin injections may lead to insulin aggregation and amyloidosis, which enhances immunogenicity, affects cell permeability, and induces apoptosis. Small molecule inhibitors designed to interfere with peptide interfacial interactions can effectively interrupt this process.
BIOORGANIC CHEMISTRY
(2021)
Article
Agriculture, Multidisciplinary
Mingkai Zhang, Ling Zhu, Hui Zhang, Xingguo Wang, Gangcheng Wu, Xiguang Qi
Summary: Currently, there is no suitable cell model to evaluate the insulinotropic effects of DPP-4 inhibitory peptides (DPP-4IPs) mediated by active GLP-1. This study used a 2D and dual-layered coculture cell model to investigate the transepithelial transport of IPYWTY and its in situ insulinotropic effects. The results showed that IPYWTY was absorbed intact and degraded during transportation, while indirectly increasing insulin levels through the stimulation of active GLP-1 secretion and DPP-4 inhibition in the coculture cell model.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Pernille Baumann Toft, Hiroaki Yashiro, Derek M. Erion, Matthew Paul Gillum, Fredrik Backhed, Tulika Arora
Summary: Depletion of gut microbiota affects dietary protein metabolism and regulates GLP-1-mediated intestinal transit, playing an important role in host physiology.
Article
Chemistry, Multidisciplinary
Leonardo F. Serafim, Vindi M. Jayasinghe-Arachchige, Lukun Wang, Parth Rathee, Jiawen Yang, N. Sreerag Moorkkannur, Rajeev Prabhakar
Summary: The selective hydrolysis of stable bonds by bio-inspired metal-based catalysts is important in various applications. Designing efficient enzyme mimics for these reactions is challenging and requires understanding chemical factors that influence catalyst activities. This study discusses the roles of catalyst-substrate complexation, non-covalent interactions, and the electronic nature of the metal ion, ligand environment, and nucleophile in various metallohydrolases and their synthetic analogues. The results will enhance the fundamental understanding of hydrolytic reactions and advance the development of computational methods for designing more efficient catalysts.
CHEMICAL COMMUNICATIONS
(2023)
Article
Plant Sciences
Xiangxiang Zhang, Yuliang Han, Xiao Han, Siqi Zhang, Liming Xiong, Tao Chen
Summary: In this study, a loss-of-function mutant dig8 was obtained through EMS mutation, which exhibited a phenotype of few lateral roots and pale green leaves. By map-based cloning, the DIG8 gene was located on AT3G62910, with a point mutation leading to amino acid substitution in the functional release factor domain. Through various experimental approaches, including yeast-two-hybrid and BiFC, it was confirmed that DIG8 protein is located in the chloroplast and interacts with ribosome-related proteins. Further analysis revealed that abnormal thylakoid stack formation and chloroplast dysfunction in the dig8 mutant led to increased ROS activity, resulting in callose deposition and decreased permeability of plasmodesmata. Local sugar supplementation partially alleviated the growth retardation phenotype of the mutant. These findings provide insights into the role of chloroplast peptide chain release factor in plant growth under ROS stress.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Jiamin Wu, Natalie Jones, Nojoud A. L. Fayez, Po-Han Chao, Angeline Wu, Daniele Ribeiro de Araujo, Elham Rouhollahi, Analisa Jia, Shyh-Dar Li
Summary: We propose a non-parenteral delivery method for proteins using protamine, an FDA-approved peptide. Protamine promotes actin tubulation and rearrangement, enhancing intracellular protein delivery compared to poly(arginine)(8) (R8). Protamine directs proteins to the nuclei with minimal lysosomal uptake, in contrast to R8-mediated delivery. Intranasal delivery of insulin mixed with protamine effectively reduces blood glucose levels in diabetic mice for up to 6 hours, similar to subcutaneously injected insulin.
JOURNAL OF CONTROLLED RELEASE
(2023)
Article
Biochemistry & Molecular Biology
Wenjuan Ni, Peiyu Zhang, Liangkun Long, Shaojun Ding
Summary: This study explored a combined strategy of protein engineering and linker-mediated co-immobilization to improve the catalytic efficiency and robustness of an artificial cascade system. The engineered TtCCO variant displayed higher activity and thermostability, and the co-immobilized TtCCO2/BaPAD system achieved a high conversion yield of ferulic acid into vanillin.
PROCESS BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Xia-Fei Wang, Zhe Chen, Xu-Bo Wang, Jin Xu, Peng Chen, Hui Ye
Summary: Research on the neuropeptide natalisin (NTL) in the common cutworm moth showed that NTL has the highest expression level in the adult stage, with similar expression patterns between the larval and pupal stages. Knocking down the expression of NTL significantly affected the reproductive behavior and output of adults.
SCIENTIFIC REPORTS
(2021)
Article
Agriculture, Multidisciplinary
Yayun Jiang, Chaochuan Zheng, Ming Jin, Ruolan Zhou, Qiaoli Wu, Fuyuan Huang, Yongliang Lou, Laibao Zheng
Summary: We developed a novel CRISPR/Cas12a mediated strand displacement/hybridization chain reaction (CSDHCR) nucleic acid assay for colorimetric detection of foodborne pathogens. The CSDHCR biosensor demonstrated satisfactory linear detection and high sensitivity for nucleic acid targets in the range of 10 fM to 1 nM, with a limit of detection of 4.54 fM. The method also showed promising specificity and sensitivity for the detection of Vibrio vulnificus, a foodborne pathogen, making it a potential alternative for ultrasensitive and visual detection of nucleic acids and foodborne pathogens.
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2023)
Article
Multidisciplinary Sciences
Tanya Clements-Decker, Marina Rautenbach, Wilma van Rensburg, Sehaam Khan, Marietjie Stander, Wesaal Khan
Summary: Secondary metabolic profiling using UPLC-MSE and molecular networking identified new stephensiolides and glucosamine derivatives produced by Serratia marcescens NP10. Genome mining revealed specific gene clusters related to these metabolites, corroborating the experimental data. Some of these compounds exhibited antibacterial activity and were found to disrupt the membrane integrity of Staphylococcus aureus.
SCIENTIFIC REPORTS
(2023)
Article
Chemistry, Multidisciplinary
Santosh Bashyal, Jo-Eun Seo, Young Wook Choi, Sangkil Lee
Summary: A novel nanocarrier platform utilizing hydrophobic ion-pairing greatly enhances intestinal absorption of oral peptide drugs. Evaluation showed that C1 outperforms C2 in oral efficacy and potential, with increased pharmacological availability. Studies suggest that HIP-nanocomplexes are effective for peptide delivery with improved intestinal absorption.
JOURNAL OF CONTROLLED RELEASE
(2021)
Article
Chemistry, Inorganic & Nuclear
Yen Jea Lee, Yujeong Kim, Haesol Kim, Jieun Choi, Ga Hee Noh, Kug-Seung Lee, Junseong Lee, Chang Hyuck Choi, Sun Hee Kim, Jiwon Seo
Summary: Replacing the imidazole moiety in the ATCUN structure with thiazole and oxazole enhances the activity of Cu(II)-ATCUN complexes and promotes the rate of ROS-mediated DNA cleavage. This study presents a new strategy for the design of peptide ligands with potential applications in the development of ROS-mediated metallodrugs.
INORGANIC CHEMISTRY
(2023)
