Review
Biotechnology & Applied Microbiology
Saadet Alpdagtas, Ossi Turunen, Jarkko Valjakka, Baris Binay
Summary: In recent years, enzymatic methods have been proposed as a green and sustainable option for CO2 conversion, offering a potential win-win strategy for producing economically useful hydrogen storage materials. NAD(+)-dependent formate dehydrogenases (FDHs) are attractive catalysts for converting CO2 into fuels and renewable chemicals. However, there is limited attention on metal-independent, NAD(+)-dependent FDHs and their CO2 reduction activity so far.
CRITICAL REVIEWS IN BIOTECHNOLOGY
(2022)
Article
Engineering, Environmental
L. Szydlowski, J. Ehlich, I. Goryanin, G. Pasternak
Summary: The study compared the electroactive capabilities of microbial communities from four mine drainages using a newly designed 96-well-plate array of microbial fuel cells. It demonstrated that the 96-well MFC array is a suitable platform for high-throughput screening, selection, and enrichment of electroactive consortia. Environmental conditions and physical and chemical parameters were found to be crucial for developing an efficient electroactive community.
CHEMICAL ENGINEERING JOURNAL
(2022)
Article
Multidisciplinary Sciences
M. Jalal Uddin, Nabil H. Bhuiyan, Joon S. Shim
Summary: By integrating programmable and disposable microfluidic pump and valve on the device, we successfully transformed the traditional 96-well ELISA assay into a point-of-care testing device, achieving individualized ELISA testing for each patient. This device significantly reduced the consumption of reagents and assay time, greatly improving the detection sensitivity, making ELISA testing more efficient and convenient.
SCIENTIFIC REPORTS
(2021)
Article
Biotechnology & Applied Microbiology
Guanhua Huang, Yanyan Han, Wei Li, Zhen Xue, Dengling He, Huan He
Summary: In this study, a 96-hole air-flowing device (96HAFD) was developed for high-throughput screening of mutant Chlorella strains. The device was confirmed to provide a homogeneous air aeration cultivation environment, and showed consistent growth characteristics with other screening devices through linear regression analysis. Compared to flask and tube air-flowing devices, the 96HAFD demonstrated higher efficiency and reliability in fast screening of individual algal colony strains, as it adapted better to the aeration cultivation conditions and exhibited faster growth. Additionally, the 96HAFD had lower water loss per day, reducing negative effects and enhancing overall system stability.
BIOPROCESS AND BIOSYSTEMS ENGINEERING
(2022)
Article
Chemistry, Multidisciplinary
Giuseppe Pietricola, Lesly Chamorro, Micaela Castellino, Diego Maureira, Tonia Tommasi, Simelys Hernandez, Lorena Wilson, Debora Fino, Carminna Ottone
Summary: This study immobilized ADH and FDH on carbon-felt-based electrodes using aldehyde groups. The compatibility of this immobilization method with electrochemical applications was studied using the ADH bioelectrode. The electrochemical regeneration process of NAD(+) on the carbon felt surface was deeply studied at different electrical potentials. The effectiveness of NAD(+) regeneration was confirmed by electrochemical oxidation of ethanol catalyzed by ADH.
Article
Chemistry, Analytical
Theresa L. Pedersen, Ira J. Gray, John W. Newman
Summary: The goal of this research was to develop a high-throughput, cost-effective method for metabolic profiling of lipid mediators and hormones involved in the regulation of inflammation and energy metabolism. The method showed low intra- and inter-batch variability for most metabolites, but higher variability for serum oxylipins and some bile acids. Application to two cohorts of elderly individuals revealed routine detection of 86 metabolites and provided valuable insights for large cohort studies.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Simeng Shao, Yihan Wang, Zhaoyang Xie, Ruiyang Xu, Xilin Wan, Enpeng Wang, Zhongxi Wang, Shuying Liu, Changbao Chen, Hongmei Yang
Summary: A novel method using competitive binding configuration and UHPLC-Orbitrap-MS was developed to screen protein binders from complicated samples. Compounds with decreased peak areas in experimental group compared to control group were detected as binders. Factors affecting binding affinity and reproducibility were optimized. The method showed potential in analyzing complex samples, with differences and similarities in lectin PNA-binding capabilities of oligosaccharides between Radix ginseng cruda and American ginseng extracts revealed for the first time.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Biochemical Research Methods
Gerrit Haeger, Johannes Bongaerts, Petra Siegert
Summary: An improved and convenient ninhydrin assay for aminoacylase activity measurements was developed using the commercial EZ NinTM reagent. The addition of DMSO enhanced the solubility of Ruhemann's purple, and the use of a basic, aqueous buffer improved its stability. Furthermore, an acidic protocol for quantification of lysine was developed. The assay allows for parallel processing in a 96-well format.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Environmental Sciences
Elijah J. Petersen, Richard Uhl, Blaza Toman, John T. Elliott, Judy Strickland, James Truax, John Gordon
Summary: The Electrophilic Allergen Screening Assay (EASA) is a promising method for detecting the first key event in the adverse outcome pathway for skin sensitization. This study describes the redesign of EASA into a 96-well plate format that incorporates in-process control measurements to increase confidence in the assay results.
Article
Multidisciplinary Sciences
Yan Chen, Jennifer Gin, Ying Wang, Markus J. de Raad, Stephen R. Tan, Nathan D. Hillson, Trent J. Northen, Paul Adams, Christopher Petzold, Faiz ul-Hassan Nasim
Summary: Plate-based proteomic sample preparation is a useful method for processing a large number of samples in the biotechnology field. It can be applied to various microbes and does not require desalting column cleanup. The protocol provides a cost-effective and environmentally-friendly option and takes approximately 30 minutes to extract protein from 96 samples. It is suitable for rapid and low-variance sample preparation of hundreds of samples.
Article
Chemistry, Analytical
Adam Szmelter, Jason Jacob, David T. Eddington
Summary: Researchers have developed a device that can deliver 12 unique oxygen concentrations to cells in a 96-well plate, allowing for in-depth studies on the effects of varying oxygen concentrations on cell responses. This technology seamlessly integrates into biomedical research workflows, demonstrating the potential for increased throughput in oxygen studies.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Ren Dodge, William B. Ludington
Summary: This article presents a method for analyzing a large number of Drosophila melanogaster microbiomes. The method utilizes a 96-well plate system and an automated counting platform to efficiently and accurately quantify microbial abundance. The method is sensitive, accurate, and faster than traditional methods.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Article
Physiology
Maria Nikulina, Travis Nemkov, Angelo D'Alessandro, Peter Gaccione, Tatsuro Yoshida
Summary: This study examines the utility of deep 96-well plates for preliminary screenings of novel red blood cell storage solutions (ASs). The results show that the plate-stored RBCs have comparable ATP and hemolysis values to the typical values observed for 6-week storage of leukoreduced AS-3 RBCs in PVC bags under both normoxic and hypoxic/hypocapnic conditions.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Endocrinology & Metabolism
Julia M. Malinowska, Taina Palosaari, Jukka Sund, Donatella Carpi, Mounir Bouhifd, Ralf J. M. Weber, Maurice Whelan, Mark R. Viant
Summary: This study established and evaluated a high-throughput metabolomics workflow compatible with a 96-well HTS platform using 50,000 HepaRG hepatocytes per well. The method showed sufficient sensitivity and repeatability, making it suitable for metabolomics-driven toxicity testing to complement HTS assays.
Article
Biochemistry & Molecular Biology
Ting Zhang, Houjin Zhang
Summary: Environmental pollution caused by heavy metals such as copper is a significant issue. Finding strains with solid tolerance to copper is crucial for microbial treatment of heavy metal pollution. Traditional methods are time-consuming and cumbersome, while this study introduces a rapid and straightforward method using mediated extracellular electron transfer to sort copper-tolerant or copper-sensitive bacteria.
BIOELECTROCHEMISTRY
(2022)
Article
Peripheral Vascular Disease
Lauriane Janssen, Laura Dupont, Mourad Bekhouche, Agnes Noel, Cedric Leduc, Marianne Voz, Bernard Peers, Didier Cataldo, Suneel S. Apte, Johanne Dubail, Alain Colige
Article
Biochemistry & Molecular Biology
Mourad Bekhouche, Alain Colige
Article
Biochemistry & Molecular Biology
Melissa L. Chow, Laura Troussicot, Marie Martin, Bastien Doumeche, Florence Guilliere, Jean-Marc Lancelin
Article
Biophysics
Cloe Desmet, Christophe A. Marquette, Loic J. Blum, Bastien Doumeche
BIOSENSORS & BIOELECTRONICS
(2016)
Article
Biochemistry & Molecular Biology
Mourad Bekhouche, Cedric Leduc, Laura Dupont, Lauriane Janssen, Frederic Delolme, Sandrine Vadon-Le Goff, Nicolas Smargiasso, Dominique Baiwir, Gabriel Mazzucchelli, Isabelle Zanella-Cleon, Johanne Dubail, Edwin De Pauw, Betty Nusgens, David J. S. Hulmes, Catherine Moali, Alain Colige
Article
Electrochemistry
Chloe Aymard, Celia Bonaventura, Robert Henkens, Christine Mousty, Laurence Hecquet, Franck Charmantray, Loic J. Blum, Bastien Doumeche
Article
Chemistry, Analytical
Chloe M. G. Aymard, Matilte Halma, Arnaud Comte, Christine Mousty, Vanessa Prevot, Laurence Hecquet, Franck Charmantray, Loic J. Blum, Bastien Doumeche
ANALYTICAL CHEMISTRY
(2018)
Article
Chemistry, Organic
Dimitri Fayolle, Nathalie Berthet, Bastien Doumeche, Olivier Renaudet, Peter Strazewski, Michele Fiore
BEILSTEIN JOURNAL OF ORGANIC CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Tamara Rosell-Garcia, Alberto Paradela, Gema Bravo, Laura Dupont, Mourad Bekhouche, Alain Colige, Fernando Rodriguez-Pascual
JOURNAL OF BIOLOGICAL CHEMISTRY
(2019)
Article
Biochemistry & Molecular Biology
Rene Buchet, Camille Tribes, Valentine Rouaix, Bastien Doumeche, Michele Fiore, Yuqing Wu, David Magne, Saida Mebarek
Summary: The study found that high levels of ATP released by cells near atherosclerosis plaques generate inorganic phosphate (Pi) instead of the mineralization inhibitor PPi, which may exacerbate plaque calcification.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biology
Nesrine Tifoun, Mourad Bekhouche, Jose M. de las Heras, Arnaud Guillaume, Sylvina Bouleau, Isabelle Guenal, Bernard Mignotte, Nathalie Le Floch
Summary: This study identified potential molecular partners of SFXN1, a mitochondrial protein, including proteins associated with neurological disorders. In silico analysis revealed biological processes linked to mitochondrial organization, electron transport chains, and transmembrane transport. Further investigation is needed to understand the significance of these interactions.
Review
Biochemistry & Molecular Biology
Marianne Leveque, Mourad Bekhouche, Jean-Christophe Farges, Audrey Aussel, Kadiatou Sy, Raphael Richert, Maxime Ducret
Summary: The success of regenerative endodontic procedures relies on various parameters including the nature of biomolecules or chemical compounds, hydrogel concentration, solubility of bioactive molecules, and apex size. The lack of knowledge regarding these parameters hinders the prediction of clinical outcomes and suggests the need for further research and development of mathematical models to predict the bioactivity of endodontic hydrogel.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Microbiology
Flavien Maucourt, Bastien Doumeche, David Chapulliot, Laurent Vallon, Sylvie Nazaret, Laurence Fraissinet-Tachet
Summary: This study identified microbial species capable of transforming polychlorinated biphenyls (PCBs) and investigated their ligninolytic enzyme activities. The activities of oxidase and peroxidase, particularly enhanced in the presence of PCBs, were found to potentially contribute to the transformation process.
Article
Materials Science, Biomaterials
M. Bekhouche, M. Bolon, F. Charriaud, M. Lamrayah, D. Da Costa, C. Primard, A. Costantini, M. Pasdeloup, S. Gobert, F. Mallein-Gerin, B. Verrier, M. Ducret, J-C Farges
JOURNAL OF MATERIALS CHEMISTRY B
(2020)
Article
Biophysics
Matilte Halma, Bastien Doumeche, Laurence Hecquet, Vanessa Prevot, Christine Mousty, Franck Charmantray
BIOSENSORS & BIOELECTRONICS
(2017)
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)
