Article
Cell Biology
Stefanie H. Korntner, Aniket Jana, Elizabeth Kinnard, Emily Leo, Timothy Beane, Xianmu Li, Rohit Sengupta, Lauren Becker, Catherine K. Kuo
Summary: Using chick embryos as a model, this study identified markers involved in limb tendon formation present in jaw tendons during embryonic development. Results suggest potential therapeutic approaches for CF tendon injuries and disorders by understanding the extracellular matrix development of jaw tendons.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Engineering, Biomedical
Madison E. Bates, Leia Troop, M. Ethan Brown, Jennifer L. Puetzer
Summary: The application of exogenous LOX protein significantly improves the mechanical performance of tissue engineered constructs, with timing of application being crucial. This study provides insights into the cellular regulation of LOX crosslinking.
ACTA BIOMATERIALIA
(2023)
Article
Biochemistry & Molecular Biology
Nataliya Migulina, Gavin Tjin, Alen Faiz, Theo Borghuis, Fenghua Zhao, Hans J. Kaper, Marit Metzlar, Eline van Dijk, Prashant K. Sharma, Wim Timens, Reinoud Gosens, Corry-Anke Brandsma, Janette K. Burgess
Summary: This study investigated the effects of smoking and COPD on LOs gene and protein expression in the airways and found that LOs alterations may be related to smoking and contribute to impaired airway function in COPD, providing potential novel targets for prevention or treatment of small airways changes in COPD.
Article
Biochemistry & Molecular Biology
Tamara Rosell-Garcia, Sergio Rivas-Munoz, Alain Colige, Fernando Rodriguez-Pascual
Summary: Members of the lysyl oxidase (LOX) family catalyze the oxidative deamination of lysine and hydroxylysine residues in collagen and elastin. Proteolysis by BMP1 and ADAMTS families regulates LOX enzymes, including LOXL1 associated with pelvic organ prolapse and pseudoexfoliation (PEX) glaucoma. This study reveals the molecular information of LOXL1 proteolysis by BMP1 and ADAMTS14, identifying the processing sites and suggesting complex regulation of LOXL1 function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Multidisciplinary
Jinwon Park, Jung Suk Kim, Geon Yang, Hobin Lee, Gayong Shim, Jaiwoo Lee, Yu Kyoung Oh
Summary: The researchers designed LOX-responsive nanoparticles to interact with the collagen matrix in the tumor microenvironment. In vitro experiments showed that these nanoparticles could bind to LOX-secreting CT26 cells, but this binding was blocked by a LOX inhibitor. In mouse experiments, the addition of the LOX inhibitor did not significantly change the antitumor efficacy of the nanoparticles. However, without the LOX inhibitor, a single administration of the nanoparticles provided sustained responsiveness to near-infrared irradiation and ablation of primary tumors.
JOURNAL OF CONTROLLED RELEASE
(2023)
Review
Biochemistry & Molecular Biology
Carolina Anazco, Janin Riedelsberger, Lorenzo Vega-Montoto, Armando Rojas
Summary: Collagen is a vital structural protein in mammals and is strengthened by complex enzymatic and non-enzymatic reactions, such as covalent cross-linking and glycation. This review article explores the amine oxidase-like activity of plant polyphenols and their potential as antiglycation agents. It also discusses the molecular mechanisms by which specific flavonoids impact or protect the normal collagen cross-linking process, with the aim of promoting the stabilization of highly organized collagen fibrils.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Thijs Koorman, Karin A. Jansen, Antoine Khalil, Peter D. Haughton, Daan Visser, Max A. K. Ratze, Wisse E. Haakma, Gabriele Sakalauskaite, Paul J. van Diest, Johan de Rooij, Patrick W. B. Derksen
Summary: The study reveals that increased collagen bundling promotes breast cancer invasion, while advanced glycation end product crosslinking prevents collective invasion. The collective invasion of ductal breast cancer cells depends on Lysyl oxidase-like 3 produced by tumor cells.
Article
Biochemistry & Molecular Biology
Qiuchen Guo, Duanchen Sun, Alexander S. Barrett, Sonali Jindal, Nathan D. Pennock, Matthew W. Conklin, Zheng Xia, Elizabeth Mitchell, Ravikant Samatham, Naomi Mirza, Steven Jacques, Sheila Weinmann, Virginia F. Borges, Kirk C. Hansen, Pepper J. Schedin
Summary: Mammographically-detected breast density has an impact on breast cancer risk and progression, and the presence of fibrillar collagen plays a crucial role in breast density. This study investigated the physiological factors influencing collagen production in the breast. Through the analysis of gene expression in female rats, the study revealed a triphasic pattern of collagen gene regulation and reproductive state-dependent composition. The findings were confirmed in human breast tissue from premenopausal women. The study also found a correlation between the collagen gene signature and poor progression-free survival in breast cancer patients. These findings contribute to a better understanding of normal breast function, the etiology of breast density, and breast cancer risk and outcomes.
Article
Cell Biology
Yinxiu Chi, Yu Chen, Wang Jiang, Wenjie Huang, Mingxing Ouyang, Lei Liu, Yan Pan, Jingjing Li, Xiangping Qu, Huijun Liu, Chi Liu, Linhong Deng, Xiaoqun Qin, Yang Xiang
Summary: ITGB4 deficiency leads to increased lung tissue stiffness, impairing the adaptation of bronchial epithelial cells to substrate stiffness and potentially associated with bronchopulmonary dysplasia.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Multidisciplinary Sciences
Sharon D'Souza, Archana Padmanabhan Nair, Ganesh Ram Sahu, Tanuja Vaidya, Rohit Shetty, Pooja Khamar, Ritika Mullick, Sneha Gupta, Mor M. Dickman, Rudy M. M. A. Nuijts, Rajiv R. Mohan, Arkasubhra Ghosh, Swaminathan Sethu
Summary: The study found significant changes in immune cell subsets and inflammatory factor profile on the ocular surface of keratoconus (KC) patients, indicating a distinct immuno-inflammatory component in KC pathogenesis. These alterations may be related to disease severity, allergy, eye rubbing, providing an additional therapeutic target for KC management.
SCIENTIFIC REPORTS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Yujia Cui, Jing Xie, Linyi Cai, Demao Zhang, Jianxun Sun, Xuedong Zhou
Summary: This study suggests that berberine repairs bone destruction caused by apical periodontics by reducing osteoclast activity, promoting osteoblast differentiation, and inhibiting inflammatory response.
Article
Dermatology
Aniqa Chowdhury, Nasim Nosoudi, Saketh Karamched, Vaideesh Parasaram, Naren Vyavahare
Summary: The combination of polyphenols with short exposure to UVA light can increase extracellular matrix deposition of elastin and collagen, potentially improving skin properties.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2021)
Article
Orthopedics
Phong K. K. Nguyen, Kaitlyn Hall, Iverson Holt, Catherine K. K. Kuo
Summary: This study suggests that treatment with recombinant LOX (rLOX) has minimal effects on the morphology and behaviors of developing tendon cells. This indicates that future rLOX-based therapeutic strategies can enhance tendon mechanical properties without adverse effects on tendon cell morphology and behaviors.
JOURNAL OF ORTHOPAEDIC RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Phong K. Nguyen, Aniket Jana, Chi Huang, Alison Grafton, Iverson Holt, Michael Giacomelli, Catherine K. Kuo
Summary: Tendon mechanical properties are compromised in adult tendon injuries and connective tissue disorders. Currently, there is no effective treatment to restore native tendon mechanical properties. However, promoting LOX-mediated collagen crosslinking may be an effective method to enhance tendon mechanical properties without toxicity.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Michael Chute, Preetinder K. Aujla, Yingxi Li, Sayantan Jana, Pavel Zhabyeyev, Jaslyn Rasmuson, Caroline A. Owen, Thomas Abraham, Gavin Y. Oudit, Zamaneh Kassiri
Summary: ADAM15 plays an important role in heart disease, and its deficiency can lead to impaired scar formation after myocardial infarction, potentially related to collagen cross-linking and kinase expression.
Article
Biochemical Research Methods
Fernando Rodriguez-Pascual, Tamara Rosell-Garcia
Summary: This review article provides a critical overview of current methods for detecting LOX activity and introduces some recent technologies that may revolutionize the approach to LOX catalysis.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Engineering, Biomedical
Yadileiny Portilla, Vladimir Mulens-Arias, Alberto Paradela, Antonio Ramos-Fernandez, Sonia Perez-Yague, M. Puerto Morales, Domingo F. Barber
Summary: This study investigated the intracellular transit of two magnetic nanoparticles (MNPs) with different surface coatings in mouse cell lines. The results showed that the coating type influenced the intracellular trafficking rate and the nature of the endolysosomes in macrophages. The MNPs with a specific coating had a slower transit rate and resulted in endolysosomes with more lytic enzymes and catalytic proteins in macrophages. These MNPs also induced more autophagic vesicles and enhanced the expression of iron metabolism-related genes and proteins.
Article
Biochemistry & Molecular Biology
Laura Guerrero, Bruno Sangro, Veronica Ambao, Jose Ignacio Granero, Antonio Ramos-Fernandez, Alberto Paradela, Fernando J. Corrales
Summary: Precision medicine aims to tailor treatments based on molecular features of diseases, with proteomics providing a way to identify disease-associated proteins. Informative biomarkers are urgently needed for early diagnosis of liver disease, and the systematic quantification of enzymes related to cellular pathways may serve as a promising biomarker for patient stratification.
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
(2022)
Article
Biochemical Research Methods
Nuria Colome, Joaquin Abian, Kerman Aloria, Jesus M. Arizmendi, Silvia Barcelo-Batllori, Sophie Braga-Lagache, Odile Burlet-Schiltz, Montse Carrascal, J. Ignacio Casal, Eduard Chicano-Galvez, Cristina Chiva, Luis Felipe Clemente, Felix Elortza, Josep M. Estanyol, Joaquin Fernandez-Irigoyen, Patricia Fernandez-Puente, Maria Jose Fidalgo, Carine Froment, Manuel Fuentes, Carlos Fuentes-Almagro, Marina Gay, Alexandre Hainard, Manfred Heller, Maria Luisa Hernandez, Nieves Ibarrola, Ibon Iloro, Thomas Kieselbach, Antonio Lario, Marie Locard-Paulet, Anabel Marina-Ramirez, Luna Martin, Esperanza Morato-Lopez, Javier Munoz, Rosana Navajas, M. Antonia Odena, Leticia Odriozola, Eliandre de Oliveira, Alberto Paradela, Carla Pasquarello, Vivian de los Rios, Cristina Ruiz-Romero, Eduard Sabido, Manuel Sanchez del Pino, Jaime Sancho, Enrique Santamaria, Christine Schaeffer-Reiss, Justine Schneider, Carolina de la Torre, M. Luz Valero, Marta Vilaseca, Shuai Wu, Linfeng Wu, Pilar Ximenez de Embun, Francesc Canals, Fernando J. Corrales
Summary: Global analysis of protein phosphorylation using mass spectrometry has become a powerful tool in biological and biomedical research, but it presents challenges such as low stoichiometry and the need for residue-specific information. The Proteomics Multicentric Experiment 11 (PME11) was launched to investigate the performance of phosphoprotein analysis protocols, with a reference sample distributed to 31 laboratories for evaluation. The results indicate the suitability of the PME11 reference sample for benchmarking and optimizing phosphoproteomics strategies while evaluating intra and inter laboratory performance.
JOURNAL OF PROTEOMICS
(2022)
Article
Biochemical Research Methods
Rosana Navajas, Antonio Ramos-Fernandez, Ignacio Herraiz, Alberto Galindo, Jose Luis Bartha, Fernando Corrales, Alberto Paradela
Summary: This study identified and validated differential exosomal proteins in liquid biopsy of pregnant women, opening up new possibilities for early detection of pre-eclampsia. In addition, the functional impact of the proteome composition of purified pre-eclamptic exosomes in target cells provides new information to better understand changes in embryo-maternal interactions and the pathogenesis of this diseas.
CLINICAL PROTEOMICS
(2022)
Article
Biochemistry & Molecular Biology
Tamara Rosell-Garcia, Sergio Rivas-Munoz, Alain Colige, Fernando Rodriguez-Pascual
Summary: Members of the lysyl oxidase (LOX) family catalyze the oxidative deamination of lysine and hydroxylysine residues in collagen and elastin. Proteolysis by BMP1 and ADAMTS families regulates LOX enzymes, including LOXL1 associated with pelvic organ prolapse and pseudoexfoliation (PEX) glaucoma. This study reveals the molecular information of LOXL1 proteolysis by BMP1 and ADAMTS14, identifying the processing sites and suggesting complex regulation of LOXL1 function.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Fatima Milhano Santos, Joana Mesquita, Joao Paulo Castro-de-Sousa, Sergio Ciordia, Alberto Paradela, Candida Teixeira Tomaz
Summary: Oxidative stress refers to the imbalance between pro-oxidants and antioxidants, leading to various retinal disorders. While the human body has defense mechanisms to counteract autoxidation, it may not always be sufficient to prevent cellular damage. Modern lifestyles and environmental factors contribute to increased chemical exposure and stress induction, further exacerbating oxidative stress.
Article
Plant Sciences
Salvador Gonzalez-Gordo, Marta Rodriguez-Ruiz, Alberto Paradela, Antonio Ramos-Fernandez, Francisco J. Corpas, Jose M. Palma
Summary: The physiological process of fruit ripening is closely related to the role of mitochondrial proteome, especially in mitochondrial electron transport chain and tricarboxylic acid cycle. Proteomic analysis revealed the key genes and pathways involved in fruit ripening. Moreover, the synthesis of antioxidant ascorbate in the mitochondrial proteome also plays a crucial role in fruit ripening.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Review
Biochemistry & Molecular Biology
Laura Guerrero, Alberto Paradela, Fernando J. Corrales
Summary: Liver diseases, including liver cancer, have a high mortality rate and the incidence is increasing. Risk factors for liver diseases include alcohol consumption, obesity, diabetes, viral infection, and genetic determinants. The reprogramming of one-carbon metabolism in liver diseases and monitoring the enzymes involved may be useful for stratification of liver disease patients and precision medicine strategies.
Article
Biology
Nesrine Tifoun, Mourad Bekhouche, Jose M. de las Heras, Arnaud Guillaume, Sylvina Bouleau, Isabelle Guenal, Bernard Mignotte, Nathalie Le Floch
Summary: This study identified potential molecular partners of SFXN1, a mitochondrial protein, including proteins associated with neurological disorders. In silico analysis revealed biological processes linked to mitochondrial organization, electron transport chains, and transmembrane transport. Further investigation is needed to understand the significance of these interactions.
Review
Cell Biology
Miguel A. Ortega, Oscar Fraile-Martinez, Cielo Garcia-Montero, Alberto Paradela, Maria Asuncion Sanchez-Gil, Sonia Rodriguez-Martin, Juan A. De Leon-Luis, Claude Pereda-Cerquella, Julia Bujan, Luis G. Guijarro, Melchor Alvarez-Mon, Natalio Garcia-Honduvilla
Summary: The human placenta plays multiple roles in pregnancy and has a significant impact on both maternal and fetal well-being. Extracellular vesicles (EVs) are crucial for the development and functioning of the placenta, as well as for communication between the mother and fetus. These EVs have been identified as potential biomarkers for various obstetric pathologies.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Nanoscience & Nanotechnology
Yadileiny Portilla, Vladimir Mulens-Arias, Neus Daviu, Alberto Paradela, Sonia Perez-Yague, Domingo F. Barber
Summary: When iron oxide nanoparticles (IONPs) come into contact with biological fluids, they form protein corona complexes that vary in composition based on the origin of the serum. These complexes have an impact on the interaction between IONPs and macrophages, ultimately affecting the immune system.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Review
Biochemistry & Molecular Biology
Marianne Leveque, Mourad Bekhouche, Jean-Christophe Farges, Audrey Aussel, Kadiatou Sy, Raphael Richert, Maxime Ducret
Summary: The success of regenerative endodontic procedures relies on various parameters including the nature of biomolecules or chemical compounds, hydrogel concentration, solubility of bioactive molecules, and apex size. The lack of knowledge regarding these parameters hinders the prediction of clinical outcomes and suggests the need for further research and development of mathematical models to predict the bioactivity of endodontic hydrogel.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)