Article
Biochemistry & Molecular Biology
Adrian Najer, Omar Rifaie-Graham, Jonathan Yeow, Christopher Adrianus, Mohamed Chami, Molly M. Stevens
Summary: Comparative studies on the interaction between polymersomes and blood plasma proteins provide key insights into the clinical translation of vesicular nanocarriers, highlighting the importance of such studies.
MACROMOLECULAR BIOSCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Subhankar Kundu, Subhadeep Das, Abhijit Patra
Summary: The properties and functions of non-covalent interaction-driven fluorescent supramolecular self-assembly depend greatly on their evolution dynamics. Electron microscopy, atomic force microscopy, and confocal laser scanning microscopy have been used to elucidate the formation of molecular self-assembly. However, some pertinent issues often impede the real-time analysis and exploration of the dynamics of molecular self-assembly processes. Fluorescence correlation spectroscopy and fluorescence lifetime imaging microscopy have recently been explored to unravel the physical picture of the in situ growth dynamics and stimuli-induced morphological transformation of luminescent self-assembled structures.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
Zhongbo Yang, Muyang Zhang, Dandan Li, Ligang Chen, Ailing Fu, Yanmei Liang, Huabin Wang
Summary: Imaging melanoma slices with THz wave-based systems revealed an artificial phenomenon that could lead to misinterpretation of experimental results. The phenomenon was found to be caused by frequency-dependent diffraction of the sample edge, as investigated experimentally and theoretically. This work is crucial for accurate interpretation of images obtained using THz-TDS technique.
BIOMEDICAL OPTICS EXPRESS
(2021)
Article
Food Science & Technology
Nelson Romano, Monica Marro, Maria Marsal, Pablo Loza-Alvarez, Andrea Gomez-Zavaglia
Summary: Dehydration of lactic acid bacteria for technological purposes leads to damage at multiple levels of bacterial cells. This study focused on how fructose-oligosaccharides (FOS) and sucrose protect Lactobacillus delbrueckii subsp. bulgaricus during vacuum-drying. Results showed that FOS and sucrose act as protective compounds, maintaining membrane integrity and preventing conformational changes in intracellular structures like DNA.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Chemistry, Analytical
Haijiao Xu, Mingjun Cai, Jing Gao, Yan Shi, Junling Chen, Qiang Wu, Jinrui Zhang, Junguang Jiang, Hongda Wang
Summary: This study investigates the effect of membrane protein density on membrane fusion by constructing different types of liposome vesicles and using dynamic fluorescence imaging. Results show that dense membrane proteins may hinder membrane fusion, laying the foundation for constructing quasi-native membrane fusion models in vitro.
Article
Biochemistry & Molecular Biology
Bjorn Paulson, Yeonhee Shin, Akimitsu Okamoto, Yeon-Mok Oh, Jun Ki Kim, Chan-Gi Pack
Summary: RNA plays a crucial role in cellular processes, making it essential to visualize and quantify its molecular dynamics directly in living cells to understand its metabolism. In this study, fluorescence correlation methods were utilized to demonstrate the visualization and quantification of interactions between ECHO probes targeting mRNA, showing changes in fluorescence brightness and diffusion coefficient upon binding. The uptake of ECHO probes in HeLa cells for mRNA detection provides new insights into intracellular mRNA metabolism.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Leigh A. Stoddart, Joelle Goulding, Stephen J. Briddon
Summary: Fluorescence correlation spectroscopy (FCS) is a quantitative spectroscopy technique that has the potential to study the function of membrane proteins, especially with the recent application of native extraction techniques. It can be routinely used in membrane protein drug discovery.
INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Mercedes Novo, Wajih Al-Soufi
Summary: Fluorescence Correlation Spectroscopy (FCS) is a powerful technique that provides comprehensive understanding of the thermodynamic, dynamic, and structural properties of supramolecular systems. By analyzing fluorescence correlation curves, FCS can reveal the diffusion term, which is related to the binding equilibrium constant and other thermodynamic properties of the supramolecular system. Whether in the fast-exchange dynamics or slow-exchange dynamics, FCS can infer the structural information of supramolecular complexes based on their diffusional properties.
FRONTIERS IN CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Nikita Kundu, Taniya Sharma, Sarvpreet Kaur, Aman Kumar Mahto, Rikeshwer Prasad Dewangan, J. Shankaraswamy, Sarika Saxena
Summary: This study provides the first evidence of human telomeric G4 destabilization upon peptide binding, which will open new research avenues for treating DNA-related diseases.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2023)
Article
Environmental Sciences
Panitan Jutaporn, Rose M. Cory, Philip C. Singer, Orlando Coronell
Summary: This study evaluated the effectiveness of five pretreatment processes in reducing UF membrane fouling in water purification, with alum and ferric chloride coagulation found to be the most effective. The research also indicated that microbially-derived DOM is a major contributor to UF membrane fouling.
Article
Multidisciplinary Sciences
Rini Ravindran, Isabel O. L. Bacellar, Xavier Castellanos-Girouard, Haytham M. M. Wahba, Zhenghao Zhang, James G. G. Omichinski, Lydia Kisley, Stephen W. W. Michnick
Summary: Peroxisomes are responsible for the ss-oxidation of fatty acids and amino acids. Dysfunction of peroxisomes can lead to both rare and prevalent diseases. The import machinery of peroxisomal proteins, which shows similarities with chloroplasts, transports folded and large protein complexes into peroxisomes. Pex13 undergoes liquid-liquid phase separation with Pex5-cargo and plays a role in the transient protein transport channels.
Article
Chemistry, Physical
Suraj Verma, Namasivayam Dhenadhayalan, King-Chuen Lin
Summary: The effect of gradual cholesterol increase on the dynamics of DOPC and DPPC membranes was investigated using single-molecule fluorescence correlation spectroscopy. The results showed that the rigidity of DOPC membranes increased with increasing cholesterol concentration, while the rigidity of DPPC membranes decreased.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Review
Polymer Science
Sascha Schmitt, Lutz Nuhn, Matthias Barz, Hans-Jurgen Butt, Kaloian Koynov
Summary: The use of nanoparticles as carriers for therapeutic agents is promising, and fluorescence correlation spectroscopy is a versatile tool for characterization of these carriers.
MACROMOLECULAR RAPID COMMUNICATIONS
(2022)
Article
Biochemistry & Molecular Biology
Guglielmo Vesco, Marta Lualdi, Mauro Fasano, Luca Nardo, Tiziana Alberio
Summary: The study provides direct evidence of binding interaction between FcRn and fibrinogen at acidic pH, suggesting a protective mechanism for fibrinogen from degradation and regulation of its plasma levels. This interaction may also impact the levels of other FcRn binding partners, including clinically relevant plasma proteins.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Johtaro Yamamoto, Akira Sasaki
Summary: The study developed a fiber-optic-based fluorescence correlation spectroscopy system that can measure the diffusion dynamics and concentration of fluorescent molecules without the need for mechanical adjustment of the experimental setup. This system was successfully applied to measure the hydrodynamic diameters and concentrations of organic fluorescent dyes and fluorescently labeled proteins.
APPLIED SCIENCES-BASEL
(2021)
Article
Biochemistry & Molecular Biology
Stefanie Hackl, Xue Wen Ng, Danqin Lu, Thorsten Wohland, Christian F. W. Becker
Summary: This study focuses on the mechanism of PrPC conversion to PrPSc, revealing that PrP-GPI exhibits specific membrane accumulation and mobility, and suggests that inhibition of actin polymerization can prevent the diffusion of PrP-GPI, thus blocking the conversion of PrPC to PrPSc.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Review
Cell Biology
Ghulam Md Ashraf, Mahmoud Ahmed Ebada, Mohd Suhail, Ashraf Ali, Md Sahab Uddin, Anwar L. Bilgrami, Asma Perveen, Amjad Husain, Mohd Tarique, Abdul Hafeez, Athanasios Alexiou, Ausaf Ahmad, Rajnish Kumar, Naheed Banu, Agnieszka Najda, Amany A. Sayed, Ghadeer M. Albadrani, Mohamed M. Abdel-Daim, Ilaria Peluso, George E. Barreto
Summary: The brain is sexually dimorphic and responds differently to pharmacological treatments for neurological diseases in males and females. Sex differences play an important role in the precise diagnosis and therapeutic strategies for neurodegenerative diseases like Alzheimer's. Understanding the impact of sex on disease mechanisms and treatment outcomes is crucial for developing effective interventions.
OXIDATIVE MEDICINE AND CELLULAR LONGEVITY
(2021)
Article
Multidisciplinary Sciences
Jagadish Sankaran, Harikrushnan Balasubramanian, Wai Hoh Tang, Xue Wen Ng, Adrian Rollin, Thorsten Wohland
Summary: This study presents an experimental strategy that optimizes spatial and temporal resolution simultaneously by implementing a GPU-supported, camera-based measurement strategy. The results demonstrate the importance of pixel-wise cross-correlation of parameters obtained from different techniques on the same data set for robust physicochemical parameter estimation and providing unique biological knowledge.
NATURE COMMUNICATIONS
(2021)
Article
Cell Biology
Xuchao Lyu, Jia Wang, Jianqin Wang, Ye-Sheng Yin, Yun Zhu, Lin-Lin Li, Shuangru Huang, Shuang Peng, Boxin Xue, Rongyu Liao, Shi-Qiang Wang, Mian Long, Thorsten Wohland, Boon Tin Chua, Yujie Sun, Pilong Li, Xiao-Wei Chen, Li Xu, Feng-Jung Chen, Peng Li
Summary: Cidec forms a gel-like condensation at LD-LD contact sites through phase separation, promoting lipid droplet fusion by generating lipid-permeable fusion plates that are geometrically constrained by donor LDs. These Cidec condensates are unevenly distributed on the fusion plate, forming stochastic sub-compartments representing unique lipid passageways during LD fusion.
DEVELOPMENTAL CELL
(2021)
Article
Multidisciplinary Sciences
Nirmalya Bag, Alice Wagenknecht-Wiesner, Allan Lee, Sophia M. Shi, David A. Holowka, Barbara A. Baird
Summary: This study evaluated the relative contributions and functional importance of different types of interactions leading to suprathreshold phosphorylation of Ag-crosslinked IgE-FceRI in live rat basophilic leukemia mast cells. The results establish a synergy of lipid-based, protein-based, and steric interactions underlying functional TM signaling in mast cells.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Pediatrics
Shahida A. Khan, Ghazi A. Damanhouri, Tahir J. Ahmed, Saeed H. Halawani, Ashraf Ali, Ahmad Makki, Sarah A. Khan
Summary: This study investigated the changes in oxidative stress and inflammatory parameters in children with sickle cell disease through supplementation with Omega-3 fatty acids. The results showed that Omega-3 fatty acids can significantly reduce inflammation and increase antioxidant defense.
JORNAL DE PEDIATRIA
(2022)
Article
Cell Biology
Alex G. Batrouni, Nirmalya Bag, Henry T. Phan, Barbara A. Baird, Jeremy M. Baskin
Summary: Phosphatidylinositol 4-kinase III alpha (PI4KIII alpha) is responsible for generating phosphatidylinositol (4)-phosphate at the plasma membrane, and the differential palmitoylation patterns of its anchor protein EFR3B control its distribution and phosphoinositide homeostasis.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Anjali Gupta, Danqin Lu, Harikrushnan Balasubramanian, Zhang Chi, Thorsten Wohland
Summary: The localization of membrane proteins within microdomains containing cholesterol and sphingolipids is crucial for cell signaling and function. However, these membrane domains are too small and dynamic to be observed with current super-resolution techniques. This study presents a simple approach to identify the phase preference of membrane-associated molecules in individual live cells within a short time. The method involves using a membrane fluidizer to induce clustering and stabilize phase separation, allowing the identification of associated molecules using routine microscopy.
JOURNAL OF LIPID RESEARCH
(2022)
Article
Chemistry, Physical
Nirmalya Bag, Erwin London, David A. Holowka, Barbara A. Baird
Summary: This article introduces the important role of phase separation in the plasma membrane and discusses the influence of transbilayer coupling on phase separation. The impact of these interactions can be quantitatively evaluated using experimental strategies and high-resolution biophysical tools, leading to the discovery of novel principles of lipid-mediated phase separation.
JOURNAL OF PHYSICAL CHEMISTRY B
(2022)
Article
Biophysics
Daniel Y. K. Aik, Thorsten Wohland
Summary: Modern EMCCD and sCMOS cameras have the capability to read out fluorescence data with single-molecule sensitivity at high frame rates. This paper presents a direct camera-read-out tool that allows access to the data in real time, simplifying alignment procedures and speeding up fluorescence experiments in microscopy.
BIOPHYSICAL JOURNAL
(2022)
Article
Biochemistry & Molecular Biology
Sarala Neomi Tantirimudalige, Palur Venkata Raghuvamsi, Kamal Kant Sharma, Jonathan Chua Wei Bao, Ganesh S. Anand, Thorsten Wohland
Summary: This study investigates the role of the cell surface molecule GM1a in dengue virus (DENV) infection. The researchers discovered that GM1a interacts with DENV, leading to increased infectivity and movement of the virus on cell surfaces. These findings suggest that GM1a functions as a coreceptor/attachment factor for DENV during infection in mammalian systems.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Ashraf Ali, Antonia Kreitlow, Madeleine Ploetz, Giovanni Normanno, Amir Abdulmawjood
Summary: A rapid and direct detection method for Thunnus albacares was developed and validated in this study, which showed high specificity and sensitivity, making it a valuable monitoring tool for food authenticity control.
Article
Biophysics
Harikrushnan Balasubramanian, Jagadish Sankaran, Shambhavi Pandey, Corinna Jie Hui Goh, Thorsten Wohland
Summary: This study investigates the oligomerization state of EGFR in cells and the factors influencing it. The results show that cholesterol, sphingolipids, and GM3-EGFR interactions can promote EGFR oligomerization, while the cytoskeleton does not have a significant effect. Moreover, mutations in the kinase and juxtamembrane domains of EGFR lead to a decrease in oligomerization, and EGFR phosphorylation is dependent on oligomerization.
BIOPHYSICAL JOURNAL
(2022)
Article
Chemistry, Multidisciplinary
Jacob A. Erstling, Nirmalya Bag, Thomas C. Gardinier, Ferdinand F. E. Kohle, Naedum Domnwachukwu, Scott D. Butler, Teresa Kao, Kai Ma, Melik Z. Turker, Grant B. Feuer, Rachel Lee, Nada Naguib, James F. Tallman, Henry F. Malarkey, Lieihn Tsaur, William L. Moore, Dana V. Chapman, Tangi Aubert, Saurabh Mehta, Richard A. Cerione, Robert S. Weiss, Barbara A. Baird, Ulrich B. Wiesner
Summary: The study examines the interactions of fluorescent and poly(ethylene glycol)-coated (PEGylated) core-shell silica nanoparticles with biological barriers during oral delivery. It finds that ultra-small nanoparticles below 10 nm diameter passively pass through tight junctions, while particles above 20 nm diameter are actively transported through cells. Additionally, the in vivo experiments in mice demonstrate successful passage through the body and renal clearance without protein corona formation, suggesting the nanoparticles as viable candidates for oral administration.
ADVANCED MATERIALS
(2023)
Review
Biology
Jagadish Sankaran, Thorsten Wohland
Summary: The Mini Review discusses recent developments in fluorescence correlation spectroscopy (FCS) and its combinations with super-resolution imaging and machine learning. FCS is a single molecule sensitive tool for quantitatively measuring biomolecular dynamics and interactions. Recent advancements in biology, computation, and detection technology enable real-time FCS experiments with multiplexed detection even in vivo.
COMMUNICATIONS BIOLOGY
(2023)
Review
Chemistry, Multidisciplinary
Dan He, Yawen Li, Fuwen Zhao, Yuze Lin
Summary: High trap density in organic solar cells leads to localized charge carriers and reduced carrier lifetime, limiting device efficiency. This feature article summarizes the recent advances of trap suppression by material design and device engineering.
CHEMICAL COMMUNICATIONS
(2024)
Review
Chemistry, Multidisciplinary
Olivier Monfort, Arshitha Madhusudhan, Martin Motola
Summary: This article presents the advantages of coupling photocatalysis and Fenton-based processes for environmental remediation and discusses their synergy. It critically examines the simultaneous triggering of these two processes and provides insights into research approaches. The study aims to enhance understanding of this complex process and highlight the potential integration of new catalysts in current wastewater treatment technology.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Manideepa Dhar, Chittaranjan Mishra, Avijit Das, Uttam Manna
Summary: An approach of 'polymerization of monomers in its aggregated form' has been introduced to tailor the water wettability of fibrous and porous substrates, achieving hydrophobicity to superhydrophobicity transition, as well as patterned wettability. This facile chemical method provides a durable coating with adjustable and patterned wettability for various potential applications.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Saswat Ranjan Bhoi, Chhanda Debnath, Shikha Gandhi
Summary: In this article, a novel catalytic reaction using Pd(0) and Bronsted acid is reported for the synthesis of biologically important imidazolidinone-fused N-heterocycles through redox-neutral intramolecular N-allylation of indoles and pyrroles with alkynes. This atom-economical method is applicable to a wide range of substrates and eliminates the need for leaving groups or oxidizing agents commonly used in traditional allylation reactions.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Longkun Chen, Mingshuai Zhang, Meichen Liu, Zhuoyuan Liu, Yuetong Qiu, Zhilai Zhang, Fuchao Yu, Jiuzhong Huang
Summary: In this study, an efficient Rh(III)-catalyzed selective mono- and dual-C-H bond functionalization/cyclization with iodonium ylide as the sole coupling partner was demonstrated. Fused benzodiazepine skeletons were obtained in excellent yields, providing an improved approach to dual C-H unsymmetrical functionalization.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Alice Kennett, Sven Epple, Gabriella van der Valk, Irene Georgiou, Evelyne Gout, Romain R. Vives, Angela J. Russell
Summary: This study presents the design and synthesis of sulfated disaccharide inhibitors based on IdoA(2S)-GlcNS(6S), which showed potent inhibition of Sulf-2. The results suggest that IdoA(2S)-GlcNS(6S) is the shortest fragment size required for effective inhibition of Sulfs, and a trisulfated disaccharide is identified as the minimal fragment size of heparan sulfate for effective endosulfatase inhibition.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Mauro Garbini, Andrea Brunetti, Riccardo Pedrazzani, Magda Monari, Massimo Marcaccio, Giulio Bertuzzi, Marco Bandini
Summary: The self-adaptability of galvanostatic electrolysis was found to be helpful in a multistage chemo- and diastereoselective electrochemically promoted cyclodimerization of chalcones. Through a series of reductive events, densely functionalized cyclopentanes with five contiguous stereocenters were obtained (25 examples, yields up to 95%, dr values up to >20:1). Further experimental and electrochemical investigations indicated the crucial role of dynamic kinetic resolution of the aldol intermediate in the reaction mechanism.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Dhananjay S. Nipate, Neha Meena, Prakash N. Swami, Krishnan Rangan, Anil Kumar
Summary: In this study, the synthesis of functionalized benzo[a]phenazines and indazolo[2,3-a]quinolines was achieved through Rh(III)-catalyzed oxidative annulation of 2-arylquinoxalines and 2-aryl-2H-indazoles with allyl alcohols. The method showed a wide substrate scope, excellent functional group tolerance, good to high yields of annulated products, and the capability for scaled-up synthesis. A tentative mechanism of the annulation reaction was proposed based on a preliminary mechanistic investigation.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Ruonan Zhang, Abudukadi Tudi, Xia Yang, Xuping Wang, Zhihua Yang, Shujuan Han, Shilie Pan
Summary: A new lead tellurium borate material with a unique fundamental building block [B4O10] was synthesized and found to exhibit high birefringence. The structure-property relationship was discussed using first-principles calculations.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Amresh Kumar Singh, Baishali Thakurta, Anupam Giri, Monalisa Pal
Summary: This study introduces three key concepts for the quick synthesis of large-scale high-quality 2D materials films and their utilization in device fabrication.
CHEMICAL COMMUNICATIONS
(2024)
Article
Chemistry, Multidisciplinary
Reina Hagiwara, Shun Nishimura, Kosuke Okeyoshi
Summary: A copolymer-conjugated nanocatalytic system has been developed to improve electron transfer and enhance photoinduced H2 generation.
CHEMICAL COMMUNICATIONS
(2024)
