Article
Microbiology
Quan Zeng, Jiaqi Liu, Zhaoyang Li, Yucan Zhang, Shaopo Zu, Xueyan Ding, Honglei Zhang
Summary: This study demonstrates that the nonstructural protein 4B (NS4B) of Japanese encephalitis virus (JEV) suppresses interferon-beta production by targeting TLR3 and TRIF proteins, thereby interfering with the host's antiviral response.
VETERINARY MICROBIOLOGY
(2023)
Article
Microbiology
Jinchao Xing, Jiaqi Liang, Shukai Liu, Lihong Huang, Pingsheng Hu, Lele Liu, Ming Liao, Wenbao Qi
Summary: High-mobility group box 1 (HMGB1) plays a critical role in Japanese encephalitis virus (JEV) propagation by suppressing the MAPK pathway, which negatively regulates JEV infection. Knock-down or knock-out of HMGB1 enhances JEV replication, while over-expression of HMGB1 restricts JEV growth in cells. These findings suggest that targeting HMGB1 could be a potential strategy for developing novel drugs to combat JEV infection.
VETERINARY MICROBIOLOGY
(2021)
Article
Microbiology
Qiang Xu, Lihong Huang, Jinchao Xing, Jiahao Zhang, Huanan Li, Lele Liu, Chen Hu, Ming Liao, Jianbo Yue, Wenbao Qi
Summary: The Japanese encephalitis virus manipulates cellular secretory pathways and autophagy components to create an optimal environment for genome replication. The virus replicates in lysosomes, not autophagosomes, and utilizes autophagic components for intracellular growth, shedding light on potential antiviral targets.
VETERINARY MICROBIOLOGY
(2021)
Article
Veterinary Sciences
Huifang Lv, Zhifeng Peng, Bingxin Jia, Huiyuan Jing, Sufang Cao, Zhikun Xu, Wang Dong
Summary: This study used RNA-seq technology to investigate the functional role of CSFV NS4A during infection. The results showed that CSFV NS4A inhibits the expression of genes related to host immune and inflammatory response, and regulates cell adhesion, apoptosis, and autophagy.
BMC VETERINARY RESEARCH
(2022)
Article
Immunology
Qiu-Yan Zhang, Si-Qing Liu, Xiao-Dan Li, Jia-Qi Li, Ya-Nan Zhang, Cheng-Lin Deng, Hong-Lei Zhang, Xu-Fang Li, Chun-Xiao Fang, Feng-Xia Yang, Bo Zhang, Yi Xu, Han-Qing Ye
Summary: This study reveals the important roles of the stem-loop and dumbbell structures in JEV replication by constructing deletion mutants. Complete deletion of the stem-loop structure significantly reduces virus replication, while the mutant with a duplicated dumbbell structure acquired by replication in mammalian cells restores virus replication capability. However, this mutant shows high attenuation in mice.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Cell Biology
Andrea Mikulasova, Leah K. Gillespie, Rebecca L. Ambrose, Turgut E. Aktepe, Alice M. Trenerry, Susann Liebscher, Jason M. Mackenzie
Summary: Flavivirus replication is closely associated with re-organized cellular membranes, forming three distinct membranous organelles with specific functions. Mutation of a putative cholesterol recognition/interaction motif within a viral protein severely impairs virus replication and the formation of replication complexes. This study highlights the crucial role of this region within the viral protein in recruiting cellular and viral proteins to promote efficient virus replication on specialized membrane platforms.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Veterinary Sciences
Jinchao Xing, Youyue Zhang, Ziying Lin, Lele Liu, Qiang Xu, Jiaqi Liang, Zhaoxia Yuan, Cuiqin Huang, Ming Liao, Wenbao Qi
Summary: Japanese encephalitis virus (JEV) is an important pathogen causing fatal neurological disease, and the study found that mutations in the 3' untranslated region (3' UTR) of different JEV strains can affect viral proliferation and pathogenicity. These findings highlight the crucial role of the 3'UTR in JEV life cycle and provide valuable insights for the development of candidate vaccines and virus production.
FRONTIERS IN VETERINARY SCIENCE
(2021)
Article
Multidisciplinary Sciences
Huan Liu, Dexi Liu
Summary: In this study, a novel SARS-CoV-2 viral vector was developed, allowing for investigation by the broader scientific community. The vector transduces target cells in an ACE2-dependent manner, and the incorporation of the VEEV self-amplification mechanism increases gene expression level and extends transgene expression time. Furthermore, the vector has significant applications in neutralizing antibody quantification and antiviral drug testing.
SCIENTIFIC REPORTS
(2023)
Article
Virology
Xiaochun Ma, Qiqi Xia, Ke Liu, Zhuanchang Wu, Chenxi Li, Changguang Xiao, Nihua Dong, Muddassar Hameed, Muhammad Naveed Anwar, Zongjie Li, Donghua Shao, Beibei Li, Yafeng Qiu, Jianchao Wei, Zhiyong Ma
Summary: In this study, it was found that palmitoylation of NS2A at the C221 residue is crucial for JEV replication and virulence. Blocking NS2A palmitoylation impaired JEV replication and attenuated its virulence. Furthermore, the mutation had no effect on NS2A's oligomerization and membrane-associated activities, but reduced protein stability and accelerated degradation through the ubiquitin-proteasome pathway.
JOURNAL OF VIROLOGY
(2023)
Article
Immunology
Wei Zhang, Miao Zeng, Bowen Jiang, Tong Lu, Jiaqi Guo, Tao Hu, Mingshu Wang, Renyong Jia, Dekang Zhu, Mafeng Liu, Xinxin Zhao, Qiao Yang, Ying Wu, Shaqiu Zhang, Xumin Ou, Yunya Liu, Ling Zhang, Yanling Yu, Leichang Pan, Anchun Cheng, Shun Chen
Summary: The study showed that overexpression of duck TMUV NS4B can competitively bind to STING, reducing TBK1 phosphorylation and inhibiting the IFN beta signaling pathway. Mutations in NS4B affected viral replication and the mortality of duck embryos to varying degrees. Targeting disabling viral strategies could be a new approach for attenuated flavivirus development.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Virology
Ya-Yun Liu, Xiao-Dong Liang, Chun-Chun Liu, Yan Cheng, Huan Chen, Abdul Sattar Baloch, Jin Zhang, Yun Young Go, Bin Zhou
Summary: The study reveals the crucial role of fatty acid synthase (FASN) in CSFV viral propagation, promoting virus proliferation by regulating lipid droplet biosynthesis and interacting with the replication complex in the endoplasmic reticulum (ER). FASN is recruited to CSFV replication sites and interacts with NS4B to regulate CSFV replication, which requires Rab18. This highlights the importance of de novo fatty acid synthesis in controlling the devastating CSFV virus.
JOURNAL OF VIROLOGY
(2021)
Review
Virology
Heidi Auerswald, Pierre-Olivier Maquart, Veronique Chevalier, Sebastien Boyer
Summary: This review summarizes the knowledge on the diversity of Japanese encephalitis virus (JEV) mosquito vector species, confirming 14 mosquito species with documented experimental vector competence for JEV. The study emphasizes the importance of conducting JEV vector competence research in local environments to achieve reliable data.
Article
Virology
Zhenjie Liang, Junhui Pan, Shengda Xie, Xingmiao Yang, Ruibing Cao
Summary: Japanese encephalitis virus (JEV) relies on hTIM-1 as an attachment factor, interacting with phosphatidylserine (PS) to promote viral infection. The study found that hTIM-1 directly interacts with JEV E protein and is co-internalized into cells with JEV during early infection stages, entering early and late endosomes. Additionally, the soluble ectodomain protein of hTIM-1 and hTIM-1-specific antibodies effectively inhibit JEV infection.
Article
Virology
Ming Jie Lim, Zhi Yang Loh, Hui Ling Yeo, Surya Pavan Yenamandra, Marcella Kong, Hao Yang Pang, Meng Han Lee, Mahathir Humaidi, Cliff Chua, Jane Griffiths, Lee Ching Ng, Hapuarachchige Chanditha Hapuarachchi, Diyar Mailepessov
Summary: This study reports the genetic characterization and first isolation of JEV from mosquito pools in Singapore. The findings indicate the presence of genotype I of JEV and suggest multiple introductions and potential establishment of an enzootic cycle in the local fauna.
Review
Virology
Baldeep Khare, Richard J. Kuhn
Summary: In the past three decades, multiple flaviviruses from different antigenic groups have spread geographically, leading to the co-circulation of multiple viruses within regions. The morphological heterogeneity of flaviviruses affects antibody recognition, virus neutralization, and infection control. Cross-reactivity among flaviviruses may result in either cross-protection or disease enhancement, although the molecular determinants and mechanisms driving these outcomes are still unclear.
Article
Virology
Xiao-Dan Li, Cheng-Lin Deng, Zhi-Ming Yuan, Han-Qing Ye, Bo Zhang
JOURNAL OF VIROLOGY
(2020)
Article
Biochemistry & Molecular Biology
Jieyu Yang, Xuping Jing, Wenfu Yi, Xiao-Dan Li, Chen Yao, Bo Zhang, Zhenhua Zheng, Hanzhong Wang, Peng Gong
Summary: Research on RNA-dependent RNA polymerases (RdRPs) in RNA viruses found that a region between RdRP catalytic motifs B and C showed host-related diversity. Substitutions in this region did not significantly affect RdRP catalytic activities but did not support virus proliferation.
NUCLEIC ACIDS RESEARCH
(2021)
Article
Microbiology
Minmin Zhou, Shaobo Wang, Jiao Guo, Yang Liu, Junyuan Cao, Xiaohao Lan, Xiaoying Jia, Bo Zhang, Gengfu Xiao, Wei Wang
Summary: PDGFR beta was identified as a potential therapeutic target against JEV infection, with the inhibitor imatinib showing protection against JEV-induced lethality by reducing viral load in the brain and preventing histopathological changes.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Biotechnology & Applied Microbiology
Qiu-Yan Zhang, Cheng-Lin Deng, Jing Liu, Jia-Qi Li, Hong-Qing Zhang, Na Li, Ya-Nan Zhang, Xiao-Dan Li, Bo Zhang, Yi Xu, Han-Qing Ye
Summary: An SARS-CoV-2 replicon was constructed for safe antiviral screening in BSL-2 laboratories, providing an effective platform for evaluating inhibitors and establishing a high-throughput screening assay. The replicon-based assay was validated using hit natural compounds from a previous cytopathic-effect-based HTS assay, demonstrating its potential for antiviral research without using live virus.
JOURNAL OF GENERAL VIROLOGY
(2021)
Article
Virology
Na Li, Zhen Wang, Rui Wang, Zhe-Rui Zhang, Ya-Nan Zhang, Cheng-Lin Deng, Bo Zhang, Lu-Qing Shang, Han-Qing Ye
Summary: Lycorine can inhibit CHIKV in cell culture without cytotoxicity and exhibits broad-spectrum anti-alphavirus activity at an early post-entry stage of CHIKV life cycle.
Article
Virology
Ya-Nan Zhang, Zhe-Rui Zhang, Na Li, Xin-Ru Pei, Xiao-Dan Li, Cheng-Lin Deng, Han-Qing Ye, Bo Zhang
Summary: This study successfully propagated Chikungunya virus in Vero cells through the construction of a chimeric VEEV-Delta C-CHIKV, significantly improving its yield. The passaged VEEV-Delta C-CHIKV demonstrated the safety and practicality of utilizing infectious capsidless viral particles of Chikungunya virus as a vaccine candidate.
JOURNAL OF VIROLOGY
(2022)
Article
Immunology
Na Li, Xiao-Ling Chen, Qi Li, Zhe-Rui Zhang, Cheng-Lin Deng, Bo Zhang, Xiao-Dan Li, Han-Qing Ye
Summary: This study establishes a more convenient and efficient method for rapid screening of inhibitors blocking syncytium formation using VEEV-SARS-CoV-2-S-eGFP replicating cells. The assay can be performed in a BSL-2 laboratory without manipulation of live SARS-CoV-2.
EMERGING MICROBES & INFECTIONS
(2022)
Article
Pharmacology & Pharmacy
Na Li, Cheng-Lin Deng, Qi Li, Xiao-Ling Chen, Bo Zhang, Han-Qing Ye
Summary: High titers of replication-defective ZIKV with NS1 deletion (ZIKV-Delta NS1) were generated using the NS1 transcomplementation system. ZIKV-Delta NS1 vaccine induced antibody responses and provided protection against ZIKV infection in mice. Moreover, ZIKV-Delta NS1 vaccination attenuated vertical transmission during pregnancy. These findings highlight the potential of the NS1 trans-complementation system for developing flavivirus vaccines.
ANTIVIRAL RESEARCH
(2023)
Correction
Virology
Xiao-Dan Li, Cheng-Lin Deng, Zhi-Ming Yuan, Han-Qing Ye, Bo Zhang
JOURNAL OF VIROLOGY
(2023)
Article
Virology
Yu-Jia Shi, Jia-Qi Li, Hong-Qing Zhang, Cheng-Lin Deng, Qin-Xuan Zhu, Bo Zhang, Xiao-Dan Li
Summary: This article describes the establishment of a high throughput screening (HTS) platform based on a recombinant Semliki Forest virus (SFV) for studying antiviral drugs against mosquito-borne alphaviruses. By using the SFV-eGFP reporter virus, a 96-well format HTS assay was successfully established and optimized, allowing rapid screening of broad-spectrum alphavirus inhibitors.
Letter
Biochemistry & Molecular Biology
Yuanyuan Li, Yanan Zhang, Yu Zhou, Yan Li, Jiao Xu, Yuanbao Ai, Lei Xu, Xiuli Xiao, Bo Zhang, Jing Jin
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2023)
Letter
Biochemistry & Molecular Biology
Hong-Qing Zhang, Ya-Nan Zhang, Zhe-Rui Zhang, Xiao-Ling Chen, Yan-Yan Hu, Yu-Jia Shi, Jing Wang, Cheng-lin Deng, Bo Zhang, Xiao-Dan Li, Han-Qing Ye
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2022)
Letter
Biochemistry & Molecular Biology
Jianxia Ou, Yanan Zhang, Yongmei Wang, Zherui Zhang, Hongping Wei, Junping Yu, Qi Wang, Guifeng Wang, Bo Zhang, Chunhe Wang
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2022)
Article
Biochemistry & Molecular Biology
Jia-Qi Li, Zhe-Rui Zhang, Hong-Qing Zhang, Ya-Nan Zhang, Xiang-Yue Zeng, Qiu-Yan Zhang, Cheng-Lin Deng, Xiao-Dan Li, Bo Zhang, Han-Qing Ye
Summary: The study demonstrated a safe and cost-effective method to prevent SARS-CoV-2 infection by expressing neutralizing antibodies in the lungs. Through in vitro and in vivo assays, it was shown that self-replicating mRNA could efficiently block SARS-CoV-2 infection with reduced viral titer and tissue damage in the lung with the help of lung target delivery using the VRP system.
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2021)
Letter
Biochemistry & Molecular Biology
Zhe-Rui Zhang, Ya-Nan Zhang, Xiao-Dan Li, Hong-Qing Zhang, Shu-Qi Xiao, Fei Deng, Zhi-Ming Yuan, Han-Qing Ye, Bo Zhang
SIGNAL TRANSDUCTION AND TARGETED THERAPY
(2020)
