Journal
CHEMICAL COMMUNICATIONS
Volume 46, Issue 1, Pages 76-78Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/b915612g
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Funding
- National Cancer Institute [1R01CA135294-01]
- Burroughs Wellcome Fund
- Stanford University National Cancer Institute Centers of Cancer Nanotechnology Excellence [1U54CA119367-01]
- Northwestern University National Cancer Institute Centers of Cancer Nanotechnology Excellence
- Korean Government [NRF-2009-352-D00098]
- National Security Science and Engineering Faculty Fellows Program award
- NATIONAL CANCER INSTITUTE [U54CA119367, R01CA135294, U54CA119341] Funding Source: NIH RePORTER
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This communication reports the use of click chemistry to site-specifically conjugate bioluminescent Renilla luciferase proteins to gold nanoparticles (Au NPs) for sensing protease activity. The bioluminescent emission from luciferase was efficiently quenched by Au NPs, but significantly recovered after the proteolytic cleavage.
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