Article
Chemistry, Multidisciplinary
Jessica Spackova, Ieva Goldberga, Rishit Yadav, Guillaume Cazals, Aurelien Lebrun, Pascal Verdie, Thomas-Xavier Metro, Danielle Laurencin
Summary: O-17 NMR spectroscopy is a powerful technique for studying biomolecules. This study presents cost-effective strategies for enriching amino acids and peptides with O-17 using mechanochemistry. The enriched samples showed high levels of enrichment with no loss of purity, making them suitable for future NMR studies.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Article
Chemistry, Multidisciplinary
Victoria Aladin, Arun K. Sreemantula, Thomas Biedenbaender, Alexander Marchanka, Bjoern Corzilius
Summary: An approach is presented to enhance NMR spectroscopic signals by site-specific enhancement of the protein-RNA binding site within a ribonucleoprotein (RNP) using dynamic nuclear polarization (DNP). This approach utilizes biochemical techniques for sparse isotope labeling and exploits the molecular dynamics of C-13-labeled methyl groups exclusively present in the protein, resulting in improved sensitivity and specificity for solid-state NMR structure determination of large biomolecules.
CHEMISTRY-A EUROPEAN JOURNAL
(2023)
Review
Biochemistry & Molecular Biology
Marie-Laure Fogeron, Lauriane Lecoq, Laura Cole, Matthias Harbers, Anja Bockmann
Summary: CFPS systems are versatile tools for basic research and product development, with WG-CFPS offering high yields for synthesizing complex eukaryotic proteins. The separation of translation reactions from cellular processes provides flexibility to adapt to protein needs. These systems have the potential to become widely used tools for recombinantly preparing challenging eukaryotic proteins.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Multidisciplinary Sciences
Sandra Bajana, Aneta Pankow, Kaili Liu, Martyna Michniowska, Joseph F. Urban, Wei R. Chen, Xiao-Hong Sun
Summary: The thymus plays a role in maintaining the homeostasis of ILC pools in tissues, especially in the absence of E protein transcription factors. ILC precursors can differentiate into various subsets in vitro, and in steady state, icCD3 epsilon(+) cells differentiate into diverse ILC subsets in different tissue environments. Helminth infection promotes their differentiation into functional ILC2s. This finding may help explain age-related differences in immune responses, as thymic activity is age-dependent.
Article
Multidisciplinary Sciences
Fan Lam, James Chu, Ji Sun Choi, Chang Cao, T. Kevin Hitchens, Scott K. Silverman, Zhi-Pei Liang, Ryan N. Dilger, Gene E. Robinson, King C. Li
Summary: This study presents a new method called epigenetic MRI (eMRI) that allows nondestructive measurement of global gene expression in the brain by directly imaging DNA methylation. The method uses C-13-enriched diets to label DNA through methionine metabolic pathways, and a C-13 magnetic resonance spectroscopic imaging technique to map the spatial distribution of labeled DNA. The study validated eMRI using pigs and discovered regional differences in global DNA methylation. The eMRI signal has the potential to enable new investigations of human brain function, behavior, and disease.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2022)
Article
Chemistry, Multidisciplinary
Amanda Pritzlaff, Guillaume Ferre, Emma Mulry, Ling Lin, Niloofar Gopal Pour, Daniel A. Savin, Michael E. Harris, Matthew T. Eddy
Summary: PEGylation is a promising method to improve protein stability, but the underlying mechanism is not well understood. This study used a structural and biophysical approach to investigate the effects of PEGylation on the carbohydrate recognition domain of human galectin-3. The results revealed the potential role of charged residues in PEG localization and provided insights for the rational design of conjugates.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Biochemistry & Molecular Biology
Tobias Stief, Lothar Gremer, Sonja Pribicevic, Delane F. Espinueva, Katharina Vormann, Ralf Biehl, Reinhard Jahn, Angel Perez-Lara, Nils -Alexander Lakomek
Summary: This study analyzed the conformation of SNAP25a in its monomeric form using NMR spectroscopy and SAXS experiments. The results showed that monomeric SNAP25a is a random coil structure with α-helices only in the N-terminal region. The study suggests that the N-terminal α-helices act as a nucleation site for SNARE complex formation.
JOURNAL OF MOLECULAR BIOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Koh Takeuchi, Imai Misaki, Yuji Tokunaga, Miwa Fujisaki, Hajime Kamoshida, Takeshi Takizawa, Hiroyuki Hanzawa, Ichio Shimada
Summary: By using solution NMR, the study compared the three-dimensional structures of cyclorasins 9A5 and 9A54, revealing that 9A5 has the ability to adopt an amphipathic structure in water, which is important for its cell permeabilization activity. On the other hand, 9A54 is unable to form this structure due to steric hindrance between bulky amino-acid sidechains at positions 2 and 3. It was also found that the bulkiness of sidechains at these positions negatively affects cell permeabilization activities.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Multidisciplinary Sciences
Jennifer Flechsler, Thomas Heimerl, Harald Huber, Reinhard Rachel, Ivan A. Berg
Summary: Prokaryotic cells are more complex than traditionally believed, with various microcompartments encapsulating metabolic enzymes or pathways. The hyperthermophilic Crenarchaeon Ignicoccus hospitalis possesses unique structures like two membranes and an unknown intermembrane compartment. The compartmentalization of metabolic pathways and information processes in this organism represents a unique example in the prokaryotic world.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Chemistry, Multidisciplinary
Giovanna Viola, Carlo Giorgio Barracchia, Roberto Tira, Francesca Parolini, Giulia Leo, Massimo Bellanda, Francesca Munari, Stefano Capaldi, Mariapina D'Onofrio, Michael Assfalg
Summary: Understanding the interactions between nanoparticles and proteins is crucial for their successful application in biology. This study reveals a new type of nano-bio interaction, where ultrasmall gold nanoparticles form stable multimolecular assemblies with intrinsically disordered proteins. This finding has important implications for the targeting of aberrant protein aggregation in cells.
Article
Biochemistry & Molecular Biology
Marlene C. S. Dal Colle, Giulio Fittolani, Martina Delbianco
Summary: NMR spectroscopy is vital for understanding the structure and dynamics of glycans. Synthetic probes with specific labels have simplified the complex NMR analysis of these compounds, providing insights into glycans' conformational aspects and their interaction with proteins.
Article
Chemistry, Multidisciplinary
Andrea Bertarello, Pierrick Berruyer, Markus Artelsmair, Charles S. Elmore, Sepideh Heydarkhan-Hagvall, Markus Schade, Elisabetta Chiarparin, Staffan Schantz, Lyndon Emsley
Summary: Determination of intracellular drug concentrations is crucial for understanding drug function and efficacy, but currently there is no experimental technique that satisfies the requirements of nondestructive measurement, no modification of drugs or targets, and detection of low concentrations. In this study, the use of magic-angle spinning dynamic nuclear polarization technique is proposed to fulfill these requirements, and successful estimation of intracellular drug amounts is demonstrated for the first time.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2022)
Article
Oncology
Elien Derveaux, Melvin Geubbelmans, Maarten Criel, Ingel Demedts, Ulrike Himpe, Kurt Tournoy, Piet Vercauter, Erik Johansson, Dirk Valkenborg, Karolien Vanhove, Liesbet Mesotten, Peter Adriaensens, Michiel Thomeer
Summary: The level of metabolic changes after complete NSCLC removal is currently unknown. By collecting multiple blood plasma samples before and after complete NSCLC removal and analyzing the plasma using NMR spectroscopy, metabolic changes can be detected. Significant changes in plasma metabolism after surgical tumor resection indicate absence of disease recurrence and can provide a good prognosis.
Article
Chemistry, Multidisciplinary
Gogulan Karunanithy, Harold W. Mackenzie, D. Flemming Hansen
Summary: The study introduces a method of virtual decoupling using deep neural networks, which enhances the resolution of nuclear magnetic resonance experiments and reduces the phase cycles required for experiments. This approach successfully decouples various protein NMR spectra, offering a flexible and effective solution where traditional methods may fail.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Veronika Slachtova, Simona Bellova, Agustina La-Venia, Juraj Galeta, Martin Dracinsky, Karel Chalupsky, Alexandra Dvorakova, Helena Mertlikova-Kaiserova, Peter Rukovansky, Rastislav Dzijak, Milan Vrabel
Summary: The development of reagents selective in complex biological media is a significant challenge. We demonstrate that N1-alkylation of 1,2,4-triazines produces more reactive triazinium salts when reacting with strained alkynes compared to the parent 1,2,4-triazines. These salts facilitate efficient modification of peptides and proteins through a powerful bioorthogonal ligation. The positively charged N1-alkyl triazinium salts possess favorable cell permeability and outperform analogous 1,2,4,5-tetrazines for intracellular fluorescent labeling applications. These new ionic heterodienes exhibit high reactivity, stability, synthetic accessibility, and improved water solubility, making them valuable additions to the repertoire of modern bioorthogonal reagents.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Biochemistry & Molecular Biology
Julia Schroeghuber, Tomas Sara, Marilena Bisaccia, Walther Schmid, Robert Konrat, Roman J. Lichtenecker
Article
Chemistry, Multidisciplinary
Kirtikumar B. Jadhav, Roman J. Lichtenecker, Anke Bullach, Bhubaneswar Mandal, Hans-Dieter Arndt
CHEMISTRY-A EUROPEAN JOURNAL
(2015)
Article
Biochemistry & Molecular Biology
Julia Schoerghuber, Leonhard Geist, Marilena Bisaccia, Frederik Weber, Robert Konrat, Roman J. Lichtenecker
JOURNAL OF BIOMOLECULAR NMR
(2017)
Article
Biochemistry & Molecular Biology
Julia Schoerghuber, Leonhard Geist, Gerald Platzer, Michael Feichtinger, Marilena Bisaccia, Lukas Scheibelberger, Frederik Weber, Robert Konrat, Roman J. Lichtenecker
JOURNAL OF BIOMOLECULAR NMR
(2018)
Article
Biochemistry & Molecular Biology
Roman J. Lichtenecker, Bernhard Ellinger, Hong-Mei Han, Kirtikumar B. Jadhav, Sascha Baumann, Oliwia Makarewicz, Markus Grabenbauer, Hans-Dieter Arndt
Article
Biochemistry & Molecular Biology
Andreas Schedlbauer, Renate Auer, Karin Ledolter, Martin Tollinger, Karin Kloiber, Roman Lichtenecker, Simon Ruedisser, Ulrich Hommel, Walther Schmid, Robert Konrat, Georg Kontaxis
JOURNAL OF BIOMOLECULAR NMR
(2008)
Article
Biochemistry & Molecular Biology
Roman J. Lichtenecker, Katharina Weinhaeupl, Lukas Reuther, Julia Schoerghuber, Walther Schmid, Robert Konrat
JOURNAL OF BIOMOLECULAR NMR
(2013)
Article
Biochemistry & Molecular Biology
Roman J. Lichtenecker, Katharina Weinhaeupl, Walther Schmid, Robert Konrat
JOURNAL OF BIOMOLECULAR NMR
(2013)
Article
Chemistry, Organic
R. J. Lichtenecker
ORGANIC & BIOMOLECULAR CHEMISTRY
(2014)
Article
Biochemistry & Molecular Biology
Kirtikumar B. Jadhav, Claudia Stein, Oliwia Makarewicz, Gabriele Pradel, Roman J. Lichtenecker, Holger Sack, Stefan H. Heinemann, Hans-Dieter Arndt
BIOORGANIC & MEDICINAL CHEMISTRY
(2017)
Article
Biochemistry & Molecular Biology
Julia Schoerghuber, Leonhard Geist, Gerald Platzer, Robert Konrat, Roman J. Lichtenecker
Article
Biochemistry & Molecular Biology
Martin Tollinger, Karin Kloiber, Bianka Agoston, Cornelia Dorigoni, Roman Lichtenecker, Walther Schmid, Robert Konrat
Article
Chemistry, Inorganic & Nuclear
E Reisner, VB Arion, M Fátima, CG da Silva, R Lichtenecker, A Eichinger, BK Keppler, VY Kukushkin, AJL Pombeiro
INORGANIC CHEMISTRY
(2004)
Article
Chemistry, Multidisciplinary
R Lichtenecker, ML Ludwiczek, W Schmid, R Konrat
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2004)
