Journal
JOURNAL OF BIOMOLECULAR NMR
Volume 57, Issue 4, Pages 327-331Publisher
SPRINGER
DOI: 10.1007/s10858-013-9796-9
Keywords
Isotope labeling; Protein NMR; Protein overexpression; Phenylalanine; Tyrosine
Categories
Funding
- FWF [P20549-N19, W-1221-B03]
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C-13-alpha-ketoacid metabolic precursors of phenylalanine and tyrosine effectively enter the metabolism of a protein overexpressing E. coli strain to label Phe- and Tyr-residues devoid of any cross-labelling. The methodology gives access to highly selective labelling patterns as valuable tools in protein NMR spectroscopy without the need of N-15-chiral amino acid synthesis using organic chemistry.
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