Achieving Regio- and Enantioselectivity of P450-Catalyzed Oxidative CH Activation of Small Functionalized Molecules by Structure-Guided Directed Evolution

A Single Active Site Mutation Inverts Stereoselectivity of 16-Hydroxylation of Testosterone Catalyzed by Engineered Cytochrome P450 BM3

(2012) Harini Venkataraman et al.   CHEMBIOCHEM

Evolving P450pyr hydroxylase for highly enantioselective hydroxylation at non-activated carbon atom

(2012) Son Quang Pham et al.   CHEMICAL COMMUNICATIONS

Plasticity of CYP2B Enzymes: Structural and Solution Biophysical Methods

(2012) P. Ross Wilderman et al.   CURRENT DRUG METABOLISM

Tuning P450 Enzymes as Oxidation Catalysts

(2012) Rudi Fasan   ACS Catalysis

If CH Bonds Could Talk: Selective CH Bond Oxidation

(2011) Timothy Newhouse et al.   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Exploration of the cytochrome c oxidase pathway puzzle and examination of the origin of elusive mutational effects

(2011) Suman Chakrabarty et al.   BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS

Cytochromes P450 as useful biocatalysts: addressing the limitations

(2011) Elaine O'Reilly et al.   CHEMICAL COMMUNICATIONS

Application of Designed Enzymes in Organic Synthesis

(2011) Gernot A. Strohmeier et al.   CHEMICAL REVIEWS

P450BM3(CYP102A1): connecting the dots

(2011) Christopher J. C. Whitehouse et al.   CHEMICAL SOCIETY REVIEWS

Development of a Concise Synthesis of (−)-Oseltamivir (Tamiflu)

(2011) Barry M. Trost et al.   CHEMISTRY-A EUROPEAN JOURNAL

Cytochrome P450: taming a wild type enzyme

(2011) Sang Taek Jung et al.   CURRENT OPINION IN BIOTECHNOLOGY

P450 Fingerprinting Method for Rapid Discovery of Terpene Hydroxylating P450 Catalysts with Diversified Regioselectivity

(2011) Kaidong Zhang et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Alkane-oxidizing metalloenzymes in the carbon cycle

(2011) Rachel Narehood Austin et al.   Metallomics

Regio- and stereoselectivity of P450-catalysed hydroxylation of steroids controlled by laboratory evolution

(2011) Sabrina Kille et al.   Nature Chemistry

Cytochrome P450 monooxygenases: an update on perspectives for synthetic application

(2011) Vlada B. Urlacher et al.   TRENDS IN BIOTECHNOLOGY

Laboratory Evolution of Stereoselective Enzymes: A Prolific Source of Catalysts for Asymmetric Reactions

(2010) Manfred T. Reetz   ANGEWANDTE CHEMIE-INTERNATIONAL EDITION

Spectroscopic characterization of cytochrome P450 Compound I

(2010) Christiane Jung et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Interactions of cytochrome P450s with their ligands

(2010) Kip P. Conner et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Spectroscopic features of cytochrome P450 reaction intermediates

(2010) Abhinav Luthra et al.   ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS

Combinatorial Alanine Substitution Enables Rapid Optimization of Cytochrome P450BM3 for Selective Hydroxylation of Large Substrates

(2010) Jared C. Lewis et al.   CHEMBIOCHEM

Inverting the enantioselectivity of P450pyr monooxygenase by directed evolution

(2010) Weng Lin Tang et al.   CHEMICAL COMMUNICATIONS

Screening of a minimal enriched P450 BM3 mutant library for hydroxylation of cyclic and acyclic alkanes

(2010) Evelyne Weber et al.   CHEMICAL COMMUNICATIONS

Enzymatic functionalization of carbon–hydrogen bonds

(2010) Jared C. Lewis et al.   CHEMICAL SOCIETY REVIEWS

Efficient stereo- and regioselective hydroxylation of alkanes catalysed by a bulky polyoxometalate

(2010) Keigo Kamata et al.   Nature Chemistry

Rational Design of a Minimal and Highly Enriched CYP102A1 Mutant Library with Improved Regio-, Stereo- and Chemoselectivity

(2009) Alexander Seifert et al.   CHEMBIOCHEM

Hydrocarbon Hydroxylation by Cytochrome P450 Enzymes

(2009) Paul R. Ortiz de Montellano   CHEMICAL REVIEWS

Advances in generating functional diversity for directed protein evolution

(2009) Amol V Shivange et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

A Highly Reactive P450 Model Compound I

(2009) Seth R. Bell et al.   JOURNAL OF THE AMERICAN CHEMICAL SOCIETY

Directed evolution drives the next generation of biocatalysts

(2009) Nicholas J Turner   Nature Chemical Biology

Enzymatic assembly of DNA molecules up to several hundred kilobases

(2009) Daniel G Gibson et al.   NATURE METHODS

Measurement of cytochrome P450 and NADPH–cytochrome P450 reductase

(2009) F Peter Guengerich et al.   Nature Protocols

Exploring protein fitness landscapes by directed evolution

(2009) Philip A. Romero et al.   NATURE REVIEWS MOLECULAR CELL BIOLOGY

Enzyme engineering for enantioselectivity: from trial-and-error to rational design?

(2009) Linda G. Otten et al.   TRENDS IN BIOTECHNOLOGY

Protein Design by Directed Evolution

(2008) Christian Jäckel et al.   Annual Review of Biophysics

Improved PCR method for the creation of saturation mutagenesis libraries in directed evolution: application to difficult-to-amplify templates

(2008) Joaquin Sanchis et al.   APPLIED MICROBIOLOGY AND BIOTECHNOLOGY

Crystal Structure of Inhibitor-Bound P450BM-3 Reveals Open Conformation of Substrate Access Channel†,‡

(2008) Donovan C. Haines et al.   BIOCHEMISTRY

Theoretical and Experimental Evaluation of a CYP106A2 Low Homology Model and Production of Mutants with Changed Activity and Selectivity of Hydroxylation

(2008) Michael Lisurek et al.   CHEMBIOCHEM

Constructing and Analyzing the Fitness Landscape of an Experimental Evolutionary Process

(2008) Manfred T. Reetz et al.   CHEMBIOCHEM

Addressing the Numbers Problem in Directed Evolution

(2008) Manfred T. Reetz et al.   CHEMBIOCHEM

Advances in laboratory evolution of enzymes

(2008) Shimon Bershtein et al.   CURRENT OPINION IN CHEMICAL BIOLOGY

Altering the regioselectivity of the subterminal fatty acid hydroxylase P450 BM-3 towards γ- and δ-positions

(2008) Matthias Dietrich et al.   JOURNAL OF BIOTECHNOLOGY

Parallel kinetic resolution of tert-butyl (RS)-3-oxy-substituted cyclopent-1-ene-carboxylates for the asymmetric synthesis of 3-oxy-substituted cispentacin and transpentacin derivatives

(2008) Yimon Aye et al.   ORGANIC & BIOMOLECULAR CHEMISTRY

The Role of the Conserved Threonine in P450BM3 Oxygen Activation: Substrate-Determined Hydroxylation Activity of the Thr268Ala Mutant

(2007) Max J. Cryle et al.   CHEMBIOCHEM

Application of drug metabolising mutants of cytochrome P450 BM3 (CYP102A1) as biocatalysts for the generation of reactive metabolites

(2007) Micaela C. Damsten et al.   CHEMICO-BIOLOGICAL INTERACTIONS

Asymmetric Synthetic Access to the Hetisine Alkaloids: Total Synthesis of (+)-Nominine

(2007) Kevin M. Peese et al.   CHEMISTRY-A EUROPEAN JOURNAL