Journal
CHEMICAL COMMUNICATIONS
Volume 48, Issue 38, Pages 4618-4620Publisher
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2cc30779k
Keywords
-
Categories
Funding
- Science & Engineering Research Council of A*STAR, Singapore [0621010024]
Ask authors/readers for more resources
Directed evolution of a monooxygenase to achieve very high enantioselectivity for hydroxylation at non-activated carbon atoms is demonstrated for the first time, where a triple mutant of P450pyr hydroxylase is obtained via determination of enzyme structure, iterative saturation mutagenesis, and high-throughput screening with a MS-based ee assay to increase the product ee from 53% to 98% for the hydroxylation of N-benzyl pyrrolidine to (S)-N-benzyl 3-hydroxypyrrolidine.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available