Article
Chemistry, Multidisciplinary
Sreya Malayam Parambath, Ashley E. Williams, Leigh Anna Hunt, Dhanashree Selvan, Nathan Hammer, Saumen Chakraborty
Summary: This study reports the construction of a de novo-designed artificial hydrogenase (ArH) inspired by [NiFe] hydrogenases, which produces H-2 gas photocatalytically and shows bell-shaped pH-dependence on activity. Spectroscopic studies elucidate the reaction mechanism and a fine balance is found between solution acidity and electron transfer steps for maximizing H-2 production.
Article
Chemistry, Multidisciplinary
Oliver J. Knowles, Linus O. Johannissen, Giacomo E. M. Crisenza, Sam Hay, David Leys, David J. Procter
Summary: This study developed a new photochemical method for the synthesis of non-natural methionines from sulfides with alkenes in water, using inexpensive riboflavin as a photocatalyst. The method allows the two-step synthesis of novel methionine analogues and the selective modification of peptide side-chains to yield peptides with non-natural methionine residues.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Pascal Puellmann, Dominik Homann, Tobias A. Karl, Burkhard Koenig, Martin J. Weissenborn
Summary: Fungal unspecific peroxygenases (UPOs) have attracted attention as versatile oxyfunctionalization catalysts with impressive catalytic capabilities. However, their sensitivity towards co-substrate hydrogen peroxide poses a challenge. This study introduces the concept of light-dependent UPO biocatalysis with genetically encoded flavin-containing photosensitizer proteins for in situ hydrogen peroxide production, enabling efficient catalysis setups and the extraction of enantiomerically pure alcohol products.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Article
Chemistry, Physical
Stefano Mazzanti, Shaowen Cao, Katharina ten Brummelhuis, Antje Voelkel, Jagadish Khamrai, Dmitry I. Sharapa, Sol Youk, Tobias Heil, Nadezda V. Tarakina, Volker Strauss, Indrajit Ghosh, Burkhard Koening, Martin Oschatz, Markus Antonietti, Aleksandr Savateev
Summary: Carbon nitrides and flavins, two easily accessible transition metal-free, photoactive materials, are combined in a photocatalytic system to increase efficiency. The non-spherical core-shell composites show enhanced performance in CO2 reduction reactions.
APPLIED CATALYSIS B-ENVIRONMENTAL
(2021)
Article
Spectroscopy
Mariana Voicescu, Oana Craciunescu, Daniel G. Angelescu, Rodica Tatia, Lucia Moldovan
Summary: This study comprehensively investigated the spectroscopic characteristics, stability, antioxidant and cytotoxic properties of FMN and FAD in biomimetic systems. Results showed significant antioxidant activity and effects on cellular viability in lipid bilayers, highlighting the importance of flavins in redox reactions and cellular respiration processes.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Physical
Andreas Rutz, Chandan K. Das, Andrea Fasano, Jan Jaenecke, Shanika Yadav, Ulf-Peter Apfel, Vera Engelbrecht, Vincent Fourmond, Christophe Leger, Lars Schaefer, Thomas Happe
Summary: The active site of [FeFe]-hydrogenases degrades upon contact with oxygen, but the protein structure of a certain hydrogenase (CbA5H) allows its active site to be protected, reducing degradation caused by oxygen. By modifying the surface residue, the hydrogenase's resistance to oxygen can be increased.
Article
Chemistry, Inorganic & Nuclear
Bingqing Liu, Yasmine S. Zubi, Jared C. Lewis
Summary: Artificial metalloenzymes (ArMs) were constructed by linking Rh(iii) polypyridyl complexes into a prolyl oligopeptidase scaffold. The properties of the resulting ArMs were studied using spectroscopic methods, and these hybrid catalysts were found to catalyze inter/intra-molecular [2 + 2] photocycloaddition in aqueous solution. The importance of cofactor/scaffold interactions for selective ArM photocatalysis was demonstrated, and the potential for controlling stereoselectivity of EnT reactions by engineering substrate binding/activation motifs in POP was highlighted.
DALTON TRANSACTIONS
(2023)
Article
Environmental Sciences
Kwena Yvonne Pete, John Kabuba, Benton Otieno, Aoyi Ochieng
Summary: A nanocomposite photocatalyst consisting of titanium dioxide supported on multiwalled carbon nanotubes was successfully prepared and used for the treatment of tetracycline-contaminated wastewater. The properties of the composite were optimized by varying the amount of titanium dioxide. The composite exhibited enhanced photocatalytic activity and followed overall pseudo-second-order kinetics and Langmuir adsorption isotherm.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2023)
Article
Chemistry, Multidisciplinary
Ramona Jurgeleit, Benjamin Grimm-Lebsanft, Benedikt Maria Floeser, Melissa Teubner, Soren Buchenau, Laura Senft, Jonas Hoffmann, Maria Naumova, Christian Naether, Ivana Ivanovic-Burmazovic, Michael Ruebhausen, Felix Tuczek
Summary: The study demonstrates that low-temperature oxygenation of copper complexes results in the formation of unique tetranuclear mixed-valent peroxo compounds, which promote O-O bond homolysis and catalytic activity, showing efficient catalytic activity towards organic substrates.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Physical
Sven T. Stripp
Summary: Earth-abundant transition metals play essential roles in cellular gas metabolism, forming gas-processing metalloenzymes that catalyze redox reactions. Biophysicists use techniques like Fourier-transform infrared spectroscopy to study the reaction principles of GPMs. Infrared spectroscopy provides information on catalytic cofactors, amino acid residues, and protein structural changes.
Article
Chemistry, Multidisciplinary
Christian Lorent, Vladimir Pelmenschikov, Stefan Frielingsdorf, Janna Schoknecht, Giorgio Caserta, Yoshitaka Yoda, Hongxin Wang, Kenji Tamasaku, Oliver Lenz, Stephen P. Cramer, Marius Horch, Lars Lauterbach, Ingo Zebger
Summary: A new experimental setup was developed to study metalloenzymes in detail, allowing the controlled preparation and characterization of catalytic intermediates using various spectroscopic techniques. By investigating two O-2-tolerant [NiFe]-hydrogenases as model systems, detailed insights into the catalytic mechanisms and vibrational fingerprints were revealed. Additionally, the study highlighted the importance of complementing X-ray crystallographic data with spectroscopic analyses.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Multidisciplinary
Jiang-Pei Yuan, Zong-Jie Guan, Heng-Yu Lin, Bing Yan, Kang-Kai Liu, Hong-Cai Zhou, Yu Fang
Summary: Mimicking the active site and substrate binding cavity of enzymes, PCCs can achieve specificity in catalytic reactions. PCC-6-M (M = Zn/Ni/Co) exhibit distinct catalytic activities, which are significant for studying the regulation of ROS generation by supramolecular catalysts.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2023)
Review
Physics, Applied
Uwe Bergmann, Jan Kern, Robert W. Schoenlein, Philippe Wernet, Vittal K. Yachandra, Junko Yano
Summary: X-ray lasers, with their tunable, intense and short pulses, offer unprecedented capabilities in studying molecular catalysts and metalloenzymes. The development of XFELs allows for powerful new probes of electronic structure dynamics, advancing our understanding of metalloenzymes. With more XFEL facilities starting to operate, new capabilities are expected in the future for real-time observation of metalloenzymes and catalysts in solution.
NATURE REVIEWS PHYSICS
(2021)
Article
Materials Science, Ceramics
Eriko Maeda, Rebecca S. Welch, Collin J. Wilkinson, John C. Mauro
Summary: The surface nucleation rates of glasses in the anorthite compositional family were predicted using molecular dynamics and the Toy Landscape Model. The addition of sodium to the base composition was tested for more complex compositions. The results were validated experimentally and computationally. This approach can guide commercial glass development more efficiently.
JOURNAL OF NON-CRYSTALLINE SOLIDS
(2023)
Article
Engineering, Environmental
Mohamed Fouad, Mohamed Gar Alalm, Hisham Kh El-Etriby, Daria Camilla Boffito, Shinichi Ookawara, Teruhisa Ohno, Manabu Fujii
Summary: The study utilized ruthenium to enhance the photocatalytic activity of a WO3/ZrO2 composite, resulting in successful disinfection of raw surface waters collected from the Nile Delta region in Egypt. The Ru/WO3/ZrO2 composite showed superior bacterial deactivation compared to the benchmark S-TiO2, with good resistance against inhibition by natural organic matter, indicating potential practical applications.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biochemistry & Molecular Biology
Nathalie Lejal, Sandrine Truchet, Edna Bechor, Edwige Bouguyon, Vijay Khedkar, Nicolas Bertho, Jasmina Vidic, Pierre Adenot, Stephanie Solier, Edgar Pick, Anny Slama-Schwok
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2018)
Article
Chemistry, Inorganic & Nuclear
Nassim Ben Brahim, Melanie Poggi, Jean-Christophe Lambry, Nairn Bel Haj Mohamed, Raft Ben Chaabane, Michel Negrerie
INORGANIC CHEMISTRY
(2018)
Article
Chemistry, Medicinal
Sebastien Dilly, Aurelien Fotso Fotso, Nathalie Lejal, Gloria Zedda, Mohamad Chebbo, Fryad Rahman, Simon Companys, Helene C. Bertrand, Jasmina Vidic, Magali Noiray, Marie-Christine Alessi, Bogdan Tarus, Stephane Quideau, Beatrice Riteau, Anny Slama-Schwok
JOURNAL OF MEDICINAL CHEMISTRY
(2018)
Article
Biochemistry & Molecular Biology
Rahima Chennoufi, Aimeric Cabrie, Nhi Ha Nguyen, Nicolas Bogliotti, Francoise Simon, Bertrand Cinquin, Patrick Tauc, Jean-Luc Boucher, Anny Slama-Schwok, Juan Xie, Eric Deprez
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS
(2019)
Article
Biochemistry & Molecular Biology
Sebastien Dilly, Linda J. Roman, Nicolas Bogliotti, Juan Xie, Eric Deprez, Anny Slama-Schwok
Article
Biochemistry & Molecular Biology
Olivier Terrier, Sebastien Dilly, Andres Pizzorno, Dominika Chalupska, Jana Humpolickova, Evzen Boura, Francis Berenbaum, Stephane Quideau, Bruno Lina, Bruno Feve, Frederic Adnet, Michele Sabbah, Manuel Rosa-Calatrava, Vincent Marechal, Julien Henri, Anny Slama-Schwok
Summary: The study investigates the efficacy of naproxen as an antiviral drug against SARS-CoV-2, finding that it can inhibit viral replication in infected cells and protect respiratory epithelium from damage. Compared to other tested drugs, naproxen combines antiviral and anti-inflammatory properties.
Article
Biochemistry & Molecular Biology
Bo Zhuang, Lipsa Nag, Pierre Sournia, Anastasia Croitoru, Rivo Ramodiharilafy, Jean-Christophe Lambry, Hannu Myllykallio, Alexey Aleksandrov, Ursula Liebl, Marten H. Vos
Summary: Quenching of flavin fluorescence by electron transfer from neighboring aromatic residues plays a significant role in flavoproteins, and can be used as a probe for the active site configuration and dynamics. Modified amino acid residues in variants can lead to changes in the temporal characteristics, indicating heterogeneous configurations of electron donor-acceptor pairs.
PHOTOCHEMICAL & PHOTOBIOLOGICAL SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Raoul Manuel, Michelle de Souza Lima, Sebastien Dilly, Sylvain Daunay, Patricia Abbe, Elodie Pramil, Stephanie Solier, Fabienne Guillaumond, Sarah-Simha Tubiana, Alexandre Escargueil, Joao Antonio Pegas Henriques, Nathalie Ferrand, Irene Erdelmeier, Jean-Luc Boucher, Gildas Bertho, Israel Agranat, Stephane Rocchi, Michele Sabbah, Anny Slama Schwok
Summary: Research on specific inhibition of NADPH oxidases (NOX) and NO-synthases (NOS) has attracted great pharmacological interest due to their association with redox stress in tumor cells. The study demonstrated that isolated 2'- and 3'-phosphate isomers of the NADPH analogue NS1 improved the specificity of inhibition by distinguishing between isomeric derivatives. The 2'-phosphate isomer of NS1 showed more pronounced effects on NOS and NOX-dependent physiological responses, with docking and molecular dynamics simulations explaining the specificity at the NADPH site of NOX and NOS.
Article
Hematology
Grigoris T. Gerotziafas, Mariella Catalano, Yiannis Theodorou, Patrick Van Dreden, Vincent Marechal, Alex C. Spyropoulos, Charles Carter, Nusrat Jabeen, Job Harenberg, Ismail Elalamy, Anna Falanga, Jawed Fareed, Petros Agathaggelou, Darko Antic, Pier Luigi Antignani, Manuel Monreal Bosch, Benjamin Brenner, Vladimir Chekhonin, Mary-Paula Colgan, Meletios-Athanasios Dimopoulos, Jim Douketis, Essam Abo Elnazar, Katalin Farkas, Bahare Fazeli, Gerry Fowkes, Yongquan Gu, Joseph Gligorov, Mark A. Ligocki, Tishya Indran, Meganathan Kannan, Bulent Kantarcioglu, Abdoul Aziz Kasse, Kostantinos Konstantinidis, Fabio Leivano, Joseph Lewis, Alexander Makatsariya, P. Massamba Mbaye, Isabelle Mahe, Irina Panovska-Stavridis, Dan-Mircea Olinic, Chryssa Papageorgiou, Zsolt Pecsvarady, Sergio Pillon, Eduardo Ramacciotti, Hikmat Abdel-Razeq, Michele Sabbah, Mouna Sassi, Gerit Schernthaner, Fakiha Siddiqui, Jin Shiomura, Anny Slama-Schwok, Jean Claude Wautrecht, Alfonso Tafur, Ali Taher, Peter Klein-Wegel, Zenguo Zhai, Tazi Mezalek Zoubida
Summary: One year after the declaration of the COVID-19 pandemic, humanity continues to face a devastating public health crisis. Non-pharmacological interventions and mass vaccination programs offer hope for ending the pandemic, but concerns remain about new viral strains and vaccine effectiveness. A balanced strategy focusing on prevention, early diagnosis, and medical care is needed to effectively manage the outbreaks and preserve global health.
THROMBOSIS AND HAEMOSTASIS
(2021)
Article
Biochemistry & Molecular Biology
Nicolas G. Biteau, Vincent Roy, Cyril Nicolas, Hubert F. Becker, Jean-Christophe Lambry, Hannu Myllykallio, Luigi A. Agrofoglio
Summary: The newly discovered FDTS is considered an attractive target for antibacterial agents against multi-resistant pathogens. A novel series of pyrido[1,2-e]purine-2,4(1H,3H)-dione analogues were synthesized and their structure-activity relationships studied, with compound 23a showing the highest inhibition.
Article
Biochemistry & Molecular Biology
Julien Henri, Laetitia Minder, Kevin Mohanasundaram, Sebastien Dilly, Anne Goupil-Lamy, Carmelo Di Primo, Anny Slama Schwok
Summary: This study identified new ligands of the nucleoprotein N of SARS-CoV-2 by in silico screening, using a refined Alphafold model of N and molecular dynamic simulations. The ligands, neuropeptides like substance P and enkephalin, impeded RNA binding to N and potentially altered the physiological roles of these neuropeptides. It was suggested that N may link viral replication and long COVID-19 symptoms, and targeted antivirals against N could improve patients' health.
Article
Biochemistry & Molecular Biology
Stephanie Solier, Michele Mondini, Lydia Meziani, Arnaud Jacquel, Catherine Lacout, Tom Vanden Berghe, Yvon Jule, Jean-Claude Martinou, Gerard Pierron, Julie Riviere, Marc Deloger, Corinne Dupuy, Anny Slama-Schwok, Nathalie Droin, Peter Vandenabeele, Patrick Auberger, Eric Deutsch, Jamel El-Benna, Pham My-Chan Dang, Eric Solary
Summary: Circulating monocytes are recruited to damaged tissues to generate macrophages that modulate disease progression. Activation of caspase-3 and caspase-7, located near mitochondria, is involved in the generation of monocyte-derived macrophages stimulated by Colony-stimulating factor-1 (CSF-1). A non-conventional pathway that involves caspases and activates NOX2 is responsible for CSF1-driven monocyte differentiation, and targeting this pathway may have therapeutic implications for modulating macrophage polarization in damaged tissues.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Jonathan Filee, Hubert f F. Becker, Lucille Mellottee, Rima Zein Eddine, Zhihui Li, Wenlu Yin, Jean-Christophe Lambry, Ursula Liebl, Hannu Myllykallio
Summary: Asgard archaea are the closest known relatives of eukaryotes. The study suggests that eukaryotic and Asgard thymidylate synthases may have a bacterial origin, and lateral transfer of bacterial genes has shaped the metabolism of Asgard archaea. The capacity of eukaryotic cells to duplicate their genetic material is a combination of archaeal and bacterial characteristics, and recent prevalent lateral gene transfer from bacteria has influenced the metabolism of Asgard archaea.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Sebastien Dilly, Miguel Romero, Stephanie Solier, Olivier Feron, Chantal Dessy, Anny Slama Schwok
Summary: ROS in cancer cells play a crucial role in regulating cell death, stemness maintenance, and metabolic reprogramming, which are associated with resistance to chemo/immunotherapy. This paper introduces new inhibitors of NADPH oxidase (NOX), which is important in the tumor microenvironment. One of the inhibitors, called Nanoshutter-1 (NS1), decreases tumor-promoting M2 macrophages in human blood monocytes. NS1 disrupts the NOX2 complex in the macrophages' membrane and mitochondria, leading to vasodilation of blood vessels and potential reduction of hypoxia. By targeting specific cells, NOX2-specific inhibitors could provide precision therapies.
Meeting Abstract
Respiratory System
Thong Hua-Huy, Carole Nicco, Jean-Luc Boucher, Hai Vu-Ngoc, Estelle Tran-Van-Hoi, Frederic Batteux, Anny Slama-Schwok, Anh Tuan Dinh-Xuan
EUROPEAN RESPIRATORY JOURNAL
(2018)